Real-time monitoring of nitric oxide and blood flow during ischemia-reperfusion in the rat testis
In the present study, we attempted to clarify the role of nitric oxide (NO) and its release during the ischemia-reperfusion rat testis. Eight-week-old male Sprague-Dawley rats were divided into seven groups: age-matched control rats, ischemia (30 minutes)-reperfusion (30 minutes) rats without NG-nit...
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| Veröffentlicht in: | Molecular and cellular biochemistry 2006-06, Vol.286 (1-2), p.139-145 |
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| creator | Kono, Tomoharu Saito, Motoaki Kinoshita, Yukako Satoh, Itaru Shinbori, Chiko Satoh, Keisuke |
| description | In the present study, we attempted to clarify the role of nitric oxide (NO) and its release during the ischemia-reperfusion rat testis. Eight-week-old male Sprague-Dawley rats were divided into seven groups: age-matched control rats, ischemia (30 minutes)-reperfusion (30 minutes) rats without NG-nitro-L-arginine methyl ester (L-NAME) and L-arginine (L-Arg) treatment, ischemia (30 minutes)-reperfusion (30 minutes) rats treated with L-NAME (10, 30, and 100 mg/kg), ischemia-reperfusion rats treated with L-Arg (10 and 30 mg/kg). Sixty minutes prior to induction of ischemia, L-NAME or L-Arg was administrated intraperitoneally. Real-time monitoring of blood flow and NO release were measured simultaneously with a laser Doppler flowmeter and an NO-selective electrode, respectively. NO2-NO3 and malonaldehyde (MDA) concentrations were measured in the experimental testes. Furthermore, we investigated possible morphological changes in the testis. Clamping of the testicular artery decreased blood flow to 5-20% of the basal level measured before clamping. Immediately following clipping of the artery, NO release rapidly increased. After removing the clip, NO release gradually returned to the basal level. This phenomenon was enhanced by treatment with L-Arg and inhibited by treatment with L-NAME. NO2-NO3 concentrations were increased by treatment with L-Arg and decreased by treatment with L-NAME, while MDA concentrations were increased by treatment with L-NAME and were decreased by treatment with L-Arg. In histological studies, the ischemia-reperfusion caused infiltration of leukocytes and a rupture of microvessels in the testis. Our data suggest that NO has cytoprotective effects on ischemia-reperfusion injury in the rat testis. |
| doi_str_mv | 10.1007/s11010-005-9105-3 |
| format | Article |
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Eight-week-old male Sprague-Dawley rats were divided into seven groups: age-matched control rats, ischemia (30 minutes)-reperfusion (30 minutes) rats without NG-nitro-L-arginine methyl ester (L-NAME) and L-arginine (L-Arg) treatment, ischemia (30 minutes)-reperfusion (30 minutes) rats treated with L-NAME (10, 30, and 100 mg/kg), ischemia-reperfusion rats treated with L-Arg (10 and 30 mg/kg). Sixty minutes prior to induction of ischemia, L-NAME or L-Arg was administrated intraperitoneally. Real-time monitoring of blood flow and NO release were measured simultaneously with a laser Doppler flowmeter and an NO-selective electrode, respectively. NO2-NO3 and malonaldehyde (MDA) concentrations were measured in the experimental testes. Furthermore, we investigated possible morphological changes in the testis. Clamping of the testicular artery decreased blood flow to 5-20% of the basal level measured before clamping. Immediately following clipping of the artery, NO release rapidly increased. After removing the clip, NO release gradually returned to the basal level. This phenomenon was enhanced by treatment with L-Arg and inhibited by treatment with L-NAME. NO2-NO3 concentrations were increased by treatment with L-Arg and decreased by treatment with L-NAME, while MDA concentrations were increased by treatment with L-NAME and were decreased by treatment with L-Arg. In histological studies, the ischemia-reperfusion caused infiltration of leukocytes and a rupture of microvessels in the testis. Our data suggest that NO has cytoprotective effects on ischemia-reperfusion injury in the rat testis.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-005-9105-3</identifier><identifier>PMID: 16496212</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Animals ; Arginine - administration & dosage ; Arginine - pharmacology ; Blood Flow Velocity - drug effects ; Blood Flow Velocity - physiology ; Enzyme Inhibitors - administration & dosage ; Enzyme Inhibitors - pharmacology ; Ischemia ; Laser-Doppler Flowmetry ; Male ; Malondialdehyde - metabolism ; Monitoring, Physiologic - methods ; NG-Nitroarginine Methyl Ester - administration & dosage ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitrates - metabolism ; Nitric oxide ; Nitric Oxide - secretion ; Nitric Oxide Synthase - antagonists & inhibitors ; Nitrites - metabolism ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - physiopathology ; Rodents ; Testis - blood supply ; Testis - drug effects ; Testis - metabolism ; Time Factors</subject><ispartof>Molecular and cellular biochemistry, 2006-06, Vol.286 (1-2), p.139-145</ispartof><rights>Springer Science+Business Media, Inc. 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-ee57cefea7c3d0b66597dad41887c07fdc5eb845e8ab6f0fa252fec6dbf0bf123</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16496212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kono, Tomoharu</creatorcontrib><creatorcontrib>Saito, Motoaki</creatorcontrib><creatorcontrib>Kinoshita, Yukako</creatorcontrib><creatorcontrib>Satoh, Itaru</creatorcontrib><creatorcontrib>Shinbori, Chiko</creatorcontrib><creatorcontrib>Satoh, Keisuke</creatorcontrib><title>Real-time monitoring of nitric oxide and blood flow during ischemia-reperfusion in the rat testis</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>In the present study, we attempted to clarify the role of nitric oxide (NO) and its release during the ischemia-reperfusion rat testis. Eight-week-old male Sprague-Dawley rats were divided into seven groups: age-matched control rats, ischemia (30 minutes)-reperfusion (30 minutes) rats without NG-nitro-L-arginine methyl ester (L-NAME) and L-arginine (L-Arg) treatment, ischemia (30 minutes)-reperfusion (30 minutes) rats treated with L-NAME (10, 30, and 100 mg/kg), ischemia-reperfusion rats treated with L-Arg (10 and 30 mg/kg). Sixty minutes prior to induction of ischemia, L-NAME or L-Arg was administrated intraperitoneally. Real-time monitoring of blood flow and NO release were measured simultaneously with a laser Doppler flowmeter and an NO-selective electrode, respectively. NO2-NO3 and malonaldehyde (MDA) concentrations were measured in the experimental testes. Furthermore, we investigated possible morphological changes in the testis. Clamping of the testicular artery decreased blood flow to 5-20% of the basal level measured before clamping. Immediately following clipping of the artery, NO release rapidly increased. After removing the clip, NO release gradually returned to the basal level. This phenomenon was enhanced by treatment with L-Arg and inhibited by treatment with L-NAME. NO2-NO3 concentrations were increased by treatment with L-Arg and decreased by treatment with L-NAME, while MDA concentrations were increased by treatment with L-NAME and were decreased by treatment with L-Arg. In histological studies, the ischemia-reperfusion caused infiltration of leukocytes and a rupture of microvessels in the testis. Our data suggest that NO has cytoprotective effects on ischemia-reperfusion injury in the rat testis.</description><subject>Animals</subject><subject>Arginine - administration & dosage</subject><subject>Arginine - pharmacology</subject><subject>Blood Flow Velocity - drug effects</subject><subject>Blood Flow Velocity - physiology</subject><subject>Enzyme Inhibitors - administration & dosage</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Ischemia</subject><subject>Laser-Doppler Flowmetry</subject><subject>Male</subject><subject>Malondialdehyde - metabolism</subject><subject>Monitoring, Physiologic - methods</subject><subject>NG-Nitroarginine Methyl Ester - administration & dosage</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitrates - metabolism</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - secretion</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Nitrites - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - physiopathology</subject><subject>Rodents</subject><subject>Testis - blood supply</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Time Factors</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkMGKFDEQhoMo7rj6AF4keNhbtKozSbqPsugqLAjinkM6qbhZujtj0o3u25txBgQvVXX4qurnY-w1wjsEMO8rIiAIACUGbEU-YTtURor9gMNTtgMJIHo05oK9qPUBGgyIz9kF6v2gO-x2zH0jN4k1zcTnvKQ1l7T84DnyNpfkef6dAnG3BD5OOQcep_yLh-0vlaq_pzk5UehAJW415YWnha_3xItb-Up1TfUlexbdVOnVuV-yu08fv19_Frdfb75cf7gVXg7dKoiU8RTJGS8DjFqrwQQX9tj3xoOJwSsa-72i3o06QnSd6iJ5HcYIY8ROXrKr091DyT-39trOLSBNk1sob9VqM3RKqiP49j_wIW9ladmsUbqDDrVqEJ4gX3KthaI9lDS78mgR7FG-Pcm3Tb49yrey7bw5H97GmcK_jbNt-QdIUYE9</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Kono, Tomoharu</creator><creator>Saito, Motoaki</creator><creator>Kinoshita, Yukako</creator><creator>Satoh, Itaru</creator><creator>Shinbori, Chiko</creator><creator>Satoh, Keisuke</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Real-time monitoring of nitric oxide and blood flow during ischemia-reperfusion in the rat testis</title><author>Kono, Tomoharu ; Saito, Motoaki ; Kinoshita, Yukako ; Satoh, Itaru ; Shinbori, Chiko ; Satoh, Keisuke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-ee57cefea7c3d0b66597dad41887c07fdc5eb845e8ab6f0fa252fec6dbf0bf123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Arginine - 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Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kono, Tomoharu</au><au>Saito, Motoaki</au><au>Kinoshita, Yukako</au><au>Satoh, Itaru</au><au>Shinbori, Chiko</au><au>Satoh, Keisuke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real-time monitoring of nitric oxide and blood flow during ischemia-reperfusion in the rat testis</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>286</volume><issue>1-2</issue><spage>139</spage><epage>145</epage><pages>139-145</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>In the present study, we attempted to clarify the role of nitric oxide (NO) and its release during the ischemia-reperfusion rat testis. Eight-week-old male Sprague-Dawley rats were divided into seven groups: age-matched control rats, ischemia (30 minutes)-reperfusion (30 minutes) rats without NG-nitro-L-arginine methyl ester (L-NAME) and L-arginine (L-Arg) treatment, ischemia (30 minutes)-reperfusion (30 minutes) rats treated with L-NAME (10, 30, and 100 mg/kg), ischemia-reperfusion rats treated with L-Arg (10 and 30 mg/kg). Sixty minutes prior to induction of ischemia, L-NAME or L-Arg was administrated intraperitoneally. Real-time monitoring of blood flow and NO release were measured simultaneously with a laser Doppler flowmeter and an NO-selective electrode, respectively. NO2-NO3 and malonaldehyde (MDA) concentrations were measured in the experimental testes. Furthermore, we investigated possible morphological changes in the testis. Clamping of the testicular artery decreased blood flow to 5-20% of the basal level measured before clamping. Immediately following clipping of the artery, NO release rapidly increased. After removing the clip, NO release gradually returned to the basal level. This phenomenon was enhanced by treatment with L-Arg and inhibited by treatment with L-NAME. NO2-NO3 concentrations were increased by treatment with L-Arg and decreased by treatment with L-NAME, while MDA concentrations were increased by treatment with L-NAME and were decreased by treatment with L-Arg. In histological studies, the ischemia-reperfusion caused infiltration of leukocytes and a rupture of microvessels in the testis. Our data suggest that NO has cytoprotective effects on ischemia-reperfusion injury in the rat testis.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>16496212</pmid><doi>10.1007/s11010-005-9105-3</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Arginine - administration & dosage Arginine - pharmacology Blood Flow Velocity - drug effects Blood Flow Velocity - physiology Enzyme Inhibitors - administration & dosage Enzyme Inhibitors - pharmacology Ischemia Laser-Doppler Flowmetry Male Malondialdehyde - metabolism Monitoring, Physiologic - methods NG-Nitroarginine Methyl Ester - administration & dosage NG-Nitroarginine Methyl Ester - pharmacology Nitrates - metabolism Nitric oxide Nitric Oxide - secretion Nitric Oxide Synthase - antagonists & inhibitors Nitrites - metabolism Rats Rats, Sprague-Dawley Reperfusion Injury - physiopathology Rodents Testis - blood supply Testis - drug effects Testis - metabolism Time Factors |
| title | Real-time monitoring of nitric oxide and blood flow during ischemia-reperfusion in the rat testis |
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