Type II restriction modification systems of Prevotella bryantii TC1-1 and Prevotella ruminicola 23 strains and their effect on the efficiency of DNA introduction via electroporation

The restriction endonucleases PbrTI and Pru2I, isoschizomers of Sau3AI and HaeIII, were partially purified and characterized from anaerobic rumen bacteria Prevotella bryantii TC1-1 and Prevotella ruminicola 23, respectively. These are the first type II restriction endonucleases discovered in strains...

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Veröffentlicht in:	FEMS microbiology letters 2005-06, Vol.247 (2), p.177-183
Hauptverfasser:	Accetto, Tomaž, Peterka, Matjaž, Avguštin, Gorazd
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Base Sequence Biological and medical sciences Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - isolation & purification Deoxyribonucleases, Type II Site-Specific - metabolism Deoxyribonucleic acid DNA DNA - metabolism E coli Edetic Acid - pharmacology Electroporation Electrotransformation Enzyme Inhibitors - pharmacology Ethylenediaminetetraacetic acids Fundamental and applied biological sciences. Psychology Gene transfer Genetic Vectors - genetics Genetic Vectors - metabolism Genetics Inserts Isoschizomers Microbiology Microorganisms Pbr TI PbrTI Plasmids Prevotella Prevotella - enzymology Pru 2I Pru2I Reaction products Rumen Strains (organisms) Substrate Specificity Transformation, Bacterial Type II restriction endonuclease
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