Specific triplex binding capacity of mixed base sequence duplex nucleic acids used for single-nucleotide polymorphism detection
Specific base recognition and binding between native double-stranded DNA (dsDNA) and complementary single-stranded DNA (ssDNA) of mixed base sequence is presented. Third-strand binding, facilitated and stabilized by a DNA intercalator, YOYO-1, occurs within 5 min at room temperature. This triplex bi...
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| Veröffentlicht in: | Genetic testing 2005-06, Vol.9 (2), p.111-120 |
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| container_title | Genetic testing |
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| creator | Daksis, Jasmine I Erikson, Glen H |
| description | Specific base recognition and binding between native double-stranded DNA (dsDNA) and complementary single-stranded DNA (ssDNA) of mixed base sequence is presented. Third-strand binding, facilitated and stabilized by a DNA intercalator, YOYO-1, occurs within 5 min at room temperature. This triplex binding capability has been used to develop a homogeneous assay that accurately detects 1-, 2-, or 3-bp mutations or deletions in the dsDNA target. Every type of 1-bp mismatch can be identified. The assay can reliably distinguish homozygous from heterozygous polymerase chain reaction (PCR)-amplified genomic dsDNA, thus providing a highly sensitive clinical diagnostic assay. |
| doi_str_mv | 10.1089/gte.2005.9.111 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1090-6576 |
| ispartof | Genetic testing, 2005-06, Vol.9 (2), p.111-120 |
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| language | eng |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Base Pairing Benzoxazoles Chromatography, High Pressure Liquid DNA DNA, Single-Stranded Fluorescent Dyes Intercalating Agents Mutation Polymorphism, Single Nucleotide Quinolinium Compounds Spectrometry, Fluorescence Time Factors |
| title | Specific triplex binding capacity of mixed base sequence duplex nucleic acids used for single-nucleotide polymorphism detection |
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