Bone marrow–derived keratinocytes are not detected in normal skin and only rarely detected in wounded skin in two different murine models
Because the ability of bone marrow–derived cells (BMDCs) to repopulate tissues and the possible mechanisms of repopulation remain controversial, we used two distinct murine models to determine whether BMDCs can repopulate epidermal keratinocytes during either steady-state homeostasis or after tissue...
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| Veröffentlicht in: | Experimental hematology 2006-05, Vol.34 (5), p.672-679 |
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| creator | Fan, Qingyuan Yee, Carole Lee Ohyama, Manabu Tock, Christine Zhang, Guofeng Darling, Thomas N. Vogel, Jonathan C. |
| description | Because the ability of bone marrow–derived cells (BMDCs) to repopulate tissues and the possible mechanisms of repopulation remain controversial, we used two distinct murine models to determine whether BMDCs can repopulate epidermal keratinocytes during either steady-state homeostasis or after tissue injury.
The accessibility of skin keratinocytes makes it an excellent tissue to assess BMDC repopulation. In the two murine models, BMDCs from either male homologous B6, 129S Rosa26 mice that constitutively express ß-galactosidase or male hemizygote C57 BL/6-Tg(ACTbEGFP)1Osb/J mice expressing enhanced green fluorescent protein were transplanted via tail vein injection into control lethally irradiated (9.5 Gy) congenic female recipients and the percentage of keratinocytes derived from the transplanted BMDCs, both with and without wounding, was carefully determined.
Analysis of bone marrow, thymus, spleen, and lymph nodes confirmed complete engraftment of donor BMDCs 6 months post–bone marrow transplantation. However, during steady-state homeostasis, bone marrow–derived keratinocytes could not be detected in the epidermis. In a skin wound-healing model, the epidermis contained only rare bone marrow–derived keratinocytes ( |
| doi_str_mv | 10.1016/j.exphem.2006.02.002 |
| format | Article |
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The accessibility of skin keratinocytes makes it an excellent tissue to assess BMDC repopulation. In the two murine models, BMDCs from either male homologous B6, 129S Rosa26 mice that constitutively express ß-galactosidase or male hemizygote C57 BL/6-Tg(ACTbEGFP)1Osb/J mice expressing enhanced green fluorescent protein were transplanted via tail vein injection into control lethally irradiated (9.5 Gy) congenic female recipients and the percentage of keratinocytes derived from the transplanted BMDCs, both with and without wounding, was carefully determined.
Analysis of bone marrow, thymus, spleen, and lymph nodes confirmed complete engraftment of donor BMDCs 6 months post–bone marrow transplantation. However, during steady-state homeostasis, bone marrow–derived keratinocytes could not be detected in the epidermis. In a skin wound-healing model, the epidermis contained only rare bone marrow–derived keratinocytes (<0.0001%) but did contain scattered bone marrow–derived Langerhans cells.
These results suggest that BMDCs do not significantly contribute to steady-state epidermal homeostasis and are not required or responsible for providing keratinocyte stem cells and keratinocyte repopulation following skin injury.</description><identifier>ISSN: 0301-472X</identifier><identifier>EISSN: 1873-2399</identifier><identifier>DOI: 10.1016/j.exphem.2006.02.002</identifier><identifier>PMID: 16647573</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Bone Marrow Cells - cytology ; Cell Fusion ; In Situ Hybridization, Fluorescence ; Keratinocytes - cytology ; Mice ; Microscopy, Electron, Transmission ; Models, Animal ; Skin - pathology ; Wound Healing ; X Chromosome ; Y Chromosome</subject><ispartof>Experimental hematology, 2006-05, Vol.34 (5), p.672-679</ispartof><rights>2006 International Society for Experimental Hematology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-8ca90920c52e73220efc95bb8b52bf7ed10a2ca75cc7055d90f029b347a7e46f3</citedby><cites>FETCH-LOGICAL-c406t-8ca90920c52e73220efc95bb8b52bf7ed10a2ca75cc7055d90f029b347a7e46f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0301472X06000634$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16647573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fan, Qingyuan</creatorcontrib><creatorcontrib>Yee, Carole Lee</creatorcontrib><creatorcontrib>Ohyama, Manabu</creatorcontrib><creatorcontrib>Tock, Christine</creatorcontrib><creatorcontrib>Zhang, Guofeng</creatorcontrib><creatorcontrib>Darling, Thomas N.</creatorcontrib><creatorcontrib>Vogel, Jonathan C.</creatorcontrib><title>Bone marrow–derived keratinocytes are not detected in normal skin and only rarely detected in wounded skin in two different murine models</title><title>Experimental hematology</title><addtitle>Exp Hematol</addtitle><description>Because the ability of bone marrow–derived cells (BMDCs) to repopulate tissues and the possible mechanisms of repopulation remain controversial, we used two distinct murine models to determine whether BMDCs can repopulate epidermal keratinocytes during either steady-state homeostasis or after tissue injury.
The accessibility of skin keratinocytes makes it an excellent tissue to assess BMDC repopulation. In the two murine models, BMDCs from either male homologous B6, 129S Rosa26 mice that constitutively express ß-galactosidase or male hemizygote C57 BL/6-Tg(ACTbEGFP)1Osb/J mice expressing enhanced green fluorescent protein were transplanted via tail vein injection into control lethally irradiated (9.5 Gy) congenic female recipients and the percentage of keratinocytes derived from the transplanted BMDCs, both with and without wounding, was carefully determined.
Analysis of bone marrow, thymus, spleen, and lymph nodes confirmed complete engraftment of donor BMDCs 6 months post–bone marrow transplantation. However, during steady-state homeostasis, bone marrow–derived keratinocytes could not be detected in the epidermis. In a skin wound-healing model, the epidermis contained only rare bone marrow–derived keratinocytes (<0.0001%) but did contain scattered bone marrow–derived Langerhans cells.
These results suggest that BMDCs do not significantly contribute to steady-state epidermal homeostasis and are not required or responsible for providing keratinocyte stem cells and keratinocyte repopulation following skin injury.</description><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>Cell Fusion</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Keratinocytes - cytology</subject><subject>Mice</subject><subject>Microscopy, Electron, Transmission</subject><subject>Models, Animal</subject><subject>Skin - pathology</subject><subject>Wound Healing</subject><subject>X Chromosome</subject><subject>Y Chromosome</subject><issn>0301-472X</issn><issn>1873-2399</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoMo7rj6D0Ry8tZtJf2RzkXQxS9Y8KLgLaSTasxsdzIm6Z2dm3eP_kN_iRlnQE9CoKrIU1XU-xLylEHNgPUvtjXe7b7iUnOAvgZeA_B7ZMMG0VS8kfI-2UADrGoF_3JBHqW0BYCuk_CQXLC-b0Unmg358Tp4pIuOMex_ff9pMbpbtPQGo87OB3PImKiOSH3I1GJGk8u386WOi55puim59pYGPx9oLGQJ_3L7sHpb0j9geXkfqHXThBF9pssa3XF_sDinx-TBpOeET87xknx---bT1fvq-uO7D1evrivTQp-rwWgJkoPpOIqGc8DJyG4ch7Hj4yTQMtDcaNEZI8rBVsIEXI5NK7TAtp-aS_L8NHcXw7cVU1aLSwbnWXsMa1K9kEwOfChgewJNDClFnNQuuqLVQTFQRxPUVp1MUEcTFHBVTChtz87z13FB-7fprHoBXp6AcjTeOowqGYfeoHWxCKdscP_f8Buy-J6L</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Fan, Qingyuan</creator><creator>Yee, Carole Lee</creator><creator>Ohyama, Manabu</creator><creator>Tock, Christine</creator><creator>Zhang, Guofeng</creator><creator>Darling, Thomas N.</creator><creator>Vogel, Jonathan C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>Bone marrow–derived keratinocytes are not detected in normal skin and only rarely detected in wounded skin in two different murine models</title><author>Fan, Qingyuan ; Yee, Carole Lee ; Ohyama, Manabu ; Tock, Christine ; Zhang, Guofeng ; Darling, Thomas N. ; Vogel, Jonathan C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-8ca90920c52e73220efc95bb8b52bf7ed10a2ca75cc7055d90f029b347a7e46f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Bone Marrow Cells - cytology</topic><topic>Cell Fusion</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Keratinocytes - cytology</topic><topic>Mice</topic><topic>Microscopy, Electron, Transmission</topic><topic>Models, Animal</topic><topic>Skin - pathology</topic><topic>Wound Healing</topic><topic>X Chromosome</topic><topic>Y Chromosome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, Qingyuan</creatorcontrib><creatorcontrib>Yee, Carole Lee</creatorcontrib><creatorcontrib>Ohyama, Manabu</creatorcontrib><creatorcontrib>Tock, Christine</creatorcontrib><creatorcontrib>Zhang, Guofeng</creatorcontrib><creatorcontrib>Darling, Thomas N.</creatorcontrib><creatorcontrib>Vogel, Jonathan C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental hematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, Qingyuan</au><au>Yee, Carole Lee</au><au>Ohyama, Manabu</au><au>Tock, Christine</au><au>Zhang, Guofeng</au><au>Darling, Thomas N.</au><au>Vogel, Jonathan C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone marrow–derived keratinocytes are not detected in normal skin and only rarely detected in wounded skin in two different murine models</atitle><jtitle>Experimental hematology</jtitle><addtitle>Exp Hematol</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>34</volume><issue>5</issue><spage>672</spage><epage>679</epage><pages>672-679</pages><issn>0301-472X</issn><eissn>1873-2399</eissn><abstract>Because the ability of bone marrow–derived cells (BMDCs) to repopulate tissues and the possible mechanisms of repopulation remain controversial, we used two distinct murine models to determine whether BMDCs can repopulate epidermal keratinocytes during either steady-state homeostasis or after tissue injury.
The accessibility of skin keratinocytes makes it an excellent tissue to assess BMDC repopulation. In the two murine models, BMDCs from either male homologous B6, 129S Rosa26 mice that constitutively express ß-galactosidase or male hemizygote C57 BL/6-Tg(ACTbEGFP)1Osb/J mice expressing enhanced green fluorescent protein were transplanted via tail vein injection into control lethally irradiated (9.5 Gy) congenic female recipients and the percentage of keratinocytes derived from the transplanted BMDCs, both with and without wounding, was carefully determined.
Analysis of bone marrow, thymus, spleen, and lymph nodes confirmed complete engraftment of donor BMDCs 6 months post–bone marrow transplantation. However, during steady-state homeostasis, bone marrow–derived keratinocytes could not be detected in the epidermis. In a skin wound-healing model, the epidermis contained only rare bone marrow–derived keratinocytes (<0.0001%) but did contain scattered bone marrow–derived Langerhans cells.
These results suggest that BMDCs do not significantly contribute to steady-state epidermal homeostasis and are not required or responsible for providing keratinocyte stem cells and keratinocyte repopulation following skin injury.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>16647573</pmid><doi>10.1016/j.exphem.2006.02.002</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bone Marrow Cells - cytology Cell Fusion In Situ Hybridization, Fluorescence Keratinocytes - cytology Mice Microscopy, Electron, Transmission Models, Animal Skin - pathology Wound Healing X Chromosome Y Chromosome |
| title | Bone marrow–derived keratinocytes are not detected in normal skin and only rarely detected in wounded skin in two different murine models |
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