Single-step purification of lipase from Burkholderia multivorans using polypropylene matrix

Lipase from Burkholderia multivorans was purified with high yields directly from fermentation broth by a single-step purification protocol involving adsorption and desorption. The crude enzyme (lyophilized powder) from B. multivorans was loaded on Accurel (Membrana, Germany), a polypropylene matrix,...

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Veröffentlicht in:	Applied microbiology and biotechnology 2005-06, Vol.67 (5), p.648-653
Hauptverfasser:	GUPTA, Namita, RATHI, Pooja, SINGH, Rajni, VINEET KUMAR GOSWAMI, GUPTA, Rani
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Butanol Acetone Adsorption Ambient temperature Biological and medical sciences Biotechnology Burkholderia - enzymology Desorption Electrophoresis, Polyacrylamide Gel Enzyme engineering Enzymes Fermentation Fractional Precipitation Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Improved methods for extraction and purification of enzymes Lipase - isolation & purification Matrix Methods. Procedures. Technologies Mucor - enzymology Octoxynol Polypropylene Polypropylenes Pseudomonas - enzymology Studies Time Factors
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