Ultra-high sensitivity multi-photon detection imaging in proteomics analyses

We report on the use of 125I and 131I labeling and of new, multicolor, multi‐photon detection (MPD) methods to routinely and quantitatively detect protein spots on two‐dimensional gel electrophoresis plates in the zeptomole to attomole range. We demonstrate that the MPD methodology can be used to de...

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Veröffentlicht in:	Proteomics (Weinheim) 2005-06, Vol.5 (9), p.2322-2330
Hauptverfasser:	Kleiner, Oliver, Price, David A., Ossetrova, Natasha, Osetrov, Sergei, Volkovitsky, Peter, Drukier, Andrzej K., Godovac-Zimmermann, Jasminka
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Color Electrophoresis, Gel, Two-Dimensional Fundamental and applied biological sciences. Psychology HeLa Cells Humans Image Processing, Computer-Assisted Imaging Iodine Radioisotopes Miscellaneous Multi-photon detection Phosphoproteins - analysis Photons Proteins Proteome - analysis Streptavidin - chemistry Two-dimensional gel electrophoresis Ultra-high sensitivity
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