Coexpression of Actin-Related Protein 2 and Wiskott-Aldrich Syndrome Family Verproline-Homologous Protein 2 in Adenocarcinoma of the Lung
Purpose: Highly invasive and metastatic cancer cells, such as adenocarcinoma of the lung cells, form irregular protrusions by assembling a branched network of actin filaments. In mammalian cells, the actin-related protein 2 and 3 (Arp2/3) complex initiates actin assembly to form lamellipodial protru...
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| Veröffentlicht in: | Clinical cancer research 2006-04, Vol.12 (8), p.2449-2454 |
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| creator | SEMBA, Seitaro IWAYA, Keiichi MATSUBAYASHI, Jun SERIZAWA, Hiromi KATABA, Hiroaki HIRANO, Takashi KATO, Harubumi MATSUOKA, Takeshi MUKAI, Kiyoshi |
| description | Purpose: Highly invasive and metastatic cancer cells, such as adenocarcinoma of the lung cells, form irregular protrusions by assembling
a branched network of actin filaments. In mammalian cells, the actin-related protein 2 and 3 (Arp2/3) complex initiates actin
assembly to form lamellipodial protrusions by binding to Wiskott-Aldrich syndrome (WASP)/WASP family verproline-homologous
protein 2 (WAVE2). In this study, colocalization of Arp2 and WAVE2 in adenocarcinoma of the lung was investigated to elucidate
its prognostic value.
Experimental Design: Immunohistochemical staining of Arp2 and WAVE2 was done on mirror sections of 115 adenocarcinomas of the lung from pathologic
stage IA to IIIA classes. Kaplan-Meier disease-free survival and overall survival curves were analyzed to determine the prognostic
significance of the coexpression of Arp2 and WAVE2.
Results: Immunoreactivity for both Arp2 and WAVE2 was detected in the same cancer cells in 78 (67.8%) of the 115 lung cancer specimens.
The proportion of cancer cells expressing both Arp2 and WAVE2 was significantly higher in cases with lymph-node metastasis
( P = 0.0046), and significantly lower in bronchioloalveolar carcinomas ( P < 0.0001). The patients whose cancer cells coexpressed them had a shorter disease-free survival time ( P < 0.0001) and overall survival time ( P < 0.0001). Multivariate Cox regression analysis revealed that coexpression of Arp2 and WAVE2 is an independent risk factor
for tumor recurrence.
Conclusions: Coexpression of Arp2 and WAVE2 is correlated with poorer patient outcome, and may be involved in the mechanism of cancer
metastasis. |
| doi_str_mv | 10.1158/1078-0432.CCR-05-2566 |
| format | Article |
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a branched network of actin filaments. In mammalian cells, the actin-related protein 2 and 3 (Arp2/3) complex initiates actin
assembly to form lamellipodial protrusions by binding to Wiskott-Aldrich syndrome (WASP)/WASP family verproline-homologous
protein 2 (WAVE2). In this study, colocalization of Arp2 and WAVE2 in adenocarcinoma of the lung was investigated to elucidate
its prognostic value.
Experimental Design: Immunohistochemical staining of Arp2 and WAVE2 was done on mirror sections of 115 adenocarcinomas of the lung from pathologic
stage IA to IIIA classes. Kaplan-Meier disease-free survival and overall survival curves were analyzed to determine the prognostic
significance of the coexpression of Arp2 and WAVE2.
Results: Immunoreactivity for both Arp2 and WAVE2 was detected in the same cancer cells in 78 (67.8%) of the 115 lung cancer specimens.
The proportion of cancer cells expressing both Arp2 and WAVE2 was significantly higher in cases with lymph-node metastasis
( P = 0.0046), and significantly lower in bronchioloalveolar carcinomas ( P < 0.0001). The patients whose cancer cells coexpressed them had a shorter disease-free survival time ( P < 0.0001) and overall survival time ( P < 0.0001). Multivariate Cox regression analysis revealed that coexpression of Arp2 and WAVE2 is an independent risk factor
for tumor recurrence.
Conclusions: Coexpression of Arp2 and WAVE2 is correlated with poorer patient outcome, and may be involved in the mechanism of cancer
metastasis.</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>DOI: 10.1158/1078-0432.CCR-05-2566</identifier><identifier>PMID: 16638851</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Actin-Related Protein 2 - biosynthesis ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Aged ; Antineoplastic agents ; Arp2/3 complex ; Biological and medical sciences ; Disease-Free Survival ; Female ; Hematologic and hematopoietic diseases ; Humans ; Hymenoptera ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunohistochemistry ; Immunopathology ; lamellipodium ; lung adenocarcinoma ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Male ; Medical sciences ; metastasis ; Multivariate Analysis ; Neoplasm Staging ; Pharmacology. Drug treatments ; Platelet diseases and coagulopathies ; Pneumology ; Prognosis ; Survival Analysis ; Tumors of the respiratory system and mediastinum ; WAVE2 ; Wiskott-Aldrich Syndrome Protein Family - biosynthesis</subject><ispartof>Clinical cancer research, 2006-04, Vol.12 (8), p.2449-2454</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c618t-5590ac30ad60de287d0bd949ec683510a92583d4d4c0c24b50fb03610d2f1a173</citedby><cites>FETCH-LOGICAL-c618t-5590ac30ad60de287d0bd949ec683510a92583d4d4c0c24b50fb03610d2f1a173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3343,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17733108$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16638851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SEMBA, Seitaro</creatorcontrib><creatorcontrib>IWAYA, Keiichi</creatorcontrib><creatorcontrib>MATSUBAYASHI, Jun</creatorcontrib><creatorcontrib>SERIZAWA, Hiromi</creatorcontrib><creatorcontrib>KATABA, Hiroaki</creatorcontrib><creatorcontrib>HIRANO, Takashi</creatorcontrib><creatorcontrib>KATO, Harubumi</creatorcontrib><creatorcontrib>MATSUOKA, Takeshi</creatorcontrib><creatorcontrib>MUKAI, Kiyoshi</creatorcontrib><title>Coexpression of Actin-Related Protein 2 and Wiskott-Aldrich Syndrome Family Verproline-Homologous Protein 2 in Adenocarcinoma of the Lung</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>Purpose: Highly invasive and metastatic cancer cells, such as adenocarcinoma of the lung cells, form irregular protrusions by assembling
a branched network of actin filaments. In mammalian cells, the actin-related protein 2 and 3 (Arp2/3) complex initiates actin
assembly to form lamellipodial protrusions by binding to Wiskott-Aldrich syndrome (WASP)/WASP family verproline-homologous
protein 2 (WAVE2). In this study, colocalization of Arp2 and WAVE2 in adenocarcinoma of the lung was investigated to elucidate
its prognostic value.
Experimental Design: Immunohistochemical staining of Arp2 and WAVE2 was done on mirror sections of 115 adenocarcinomas of the lung from pathologic
stage IA to IIIA classes. Kaplan-Meier disease-free survival and overall survival curves were analyzed to determine the prognostic
significance of the coexpression of Arp2 and WAVE2.
Results: Immunoreactivity for both Arp2 and WAVE2 was detected in the same cancer cells in 78 (67.8%) of the 115 lung cancer specimens.
The proportion of cancer cells expressing both Arp2 and WAVE2 was significantly higher in cases with lymph-node metastasis
( P = 0.0046), and significantly lower in bronchioloalveolar carcinomas ( P < 0.0001). The patients whose cancer cells coexpressed them had a shorter disease-free survival time ( P < 0.0001) and overall survival time ( P < 0.0001). Multivariate Cox regression analysis revealed that coexpression of Arp2 and WAVE2 is an independent risk factor
for tumor recurrence.
Conclusions: Coexpression of Arp2 and WAVE2 is correlated with poorer patient outcome, and may be involved in the mechanism of cancer
metastasis.</description><subject>Actin-Related Protein 2 - biosynthesis</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Aged</subject><subject>Antineoplastic agents</subject><subject>Arp2/3 complex</subject><subject>Biological and medical sciences</subject><subject>Disease-Free Survival</subject><subject>Female</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Hymenoptera</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunohistochemistry</subject><subject>Immunopathology</subject><subject>lamellipodium</subject><subject>lung adenocarcinoma</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>metastasis</subject><subject>Multivariate Analysis</subject><subject>Neoplasm Staging</subject><subject>Pharmacology. Drug treatments</subject><subject>Platelet diseases and coagulopathies</subject><subject>Pneumology</subject><subject>Prognosis</subject><subject>Survival Analysis</subject><subject>Tumors of the respiratory system and mediastinum</subject><subject>WAVE2</subject><subject>Wiskott-Aldrich Syndrome Protein Family - biosynthesis</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRSMEYh7wCSBvQLPx4EfsOMtWxDBILYGG19Jy25WOIbF77LSgP4G_xlE3GnZsyrU4VbfkU1UvKLmmVKg3lDQKk5qz6667w0RgJqR8VJ1TIRrMmRSPS_-XOasucv5OCK0pqZ9WZ1RKrpSg59XvLsKvXYKcfQwo9mhlZx_wHYxmBoc-pjiDD4ghExz65vOPOM94Nbrk7YA-HYJLcQJ0YyY_HtBXSLsURx8A38YpjnEb9_mfHaWsHIRoTbI-xMksgfMAaL0P22fVk96MGZ6f3svqy83bz90tXn94975brbGVVM1YiJYYy4lxkjhgqnFk49q6BSsVF5SYlgnFXe1qSyyrN4L0G8IlJY711NCGX1avj3vLqfd7yLOefLYwjiZAOVfLpi08Z_8FaUNr1XBeQHEEbYo5J-j1LvnJpIOmRC-y9CJCLyJ0kaWJ0IusMvfyFLDfTOAepk52CvDqBJhszdgnE6zPD1xT0ilRhbs6coPfDj99Am0LCalohfLVg6ZMK83quuV_AM1Uq4o</recordid><startdate>20060415</startdate><enddate>20060415</enddate><creator>SEMBA, Seitaro</creator><creator>IWAYA, Keiichi</creator><creator>MATSUBAYASHI, Jun</creator><creator>SERIZAWA, Hiromi</creator><creator>KATABA, Hiroaki</creator><creator>HIRANO, Takashi</creator><creator>KATO, Harubumi</creator><creator>MATSUOKA, Takeshi</creator><creator>MUKAI, Kiyoshi</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20060415</creationdate><title>Coexpression of Actin-Related Protein 2 and Wiskott-Aldrich Syndrome Family Verproline-Homologous Protein 2 in Adenocarcinoma of the Lung</title><author>SEMBA, Seitaro ; IWAYA, Keiichi ; MATSUBAYASHI, Jun ; SERIZAWA, Hiromi ; KATABA, Hiroaki ; HIRANO, Takashi ; KATO, Harubumi ; MATSUOKA, Takeshi ; MUKAI, Kiyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c618t-5590ac30ad60de287d0bd949ec683510a92583d4d4c0c24b50fb03610d2f1a173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Actin-Related Protein 2 - biosynthesis</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Aged</topic><topic>Antineoplastic agents</topic><topic>Arp2/3 complex</topic><topic>Biological and medical sciences</topic><topic>Disease-Free Survival</topic><topic>Female</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Hymenoptera</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunohistochemistry</topic><topic>Immunopathology</topic><topic>lamellipodium</topic><topic>lung adenocarcinoma</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>metastasis</topic><topic>Multivariate Analysis</topic><topic>Neoplasm Staging</topic><topic>Pharmacology. Drug treatments</topic><topic>Platelet diseases and coagulopathies</topic><topic>Pneumology</topic><topic>Prognosis</topic><topic>Survival Analysis</topic><topic>Tumors of the respiratory system and mediastinum</topic><topic>WAVE2</topic><topic>Wiskott-Aldrich Syndrome Protein Family - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SEMBA, Seitaro</creatorcontrib><creatorcontrib>IWAYA, Keiichi</creatorcontrib><creatorcontrib>MATSUBAYASHI, Jun</creatorcontrib><creatorcontrib>SERIZAWA, Hiromi</creatorcontrib><creatorcontrib>KATABA, Hiroaki</creatorcontrib><creatorcontrib>HIRANO, Takashi</creatorcontrib><creatorcontrib>KATO, Harubumi</creatorcontrib><creatorcontrib>MATSUOKA, Takeshi</creatorcontrib><creatorcontrib>MUKAI, Kiyoshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SEMBA, Seitaro</au><au>IWAYA, Keiichi</au><au>MATSUBAYASHI, Jun</au><au>SERIZAWA, Hiromi</au><au>KATABA, Hiroaki</au><au>HIRANO, Takashi</au><au>KATO, Harubumi</au><au>MATSUOKA, Takeshi</au><au>MUKAI, Kiyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coexpression of Actin-Related Protein 2 and Wiskott-Aldrich Syndrome Family Verproline-Homologous Protein 2 in Adenocarcinoma of the Lung</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>2006-04-15</date><risdate>2006</risdate><volume>12</volume><issue>8</issue><spage>2449</spage><epage>2454</epage><pages>2449-2454</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><abstract>Purpose: Highly invasive and metastatic cancer cells, such as adenocarcinoma of the lung cells, form irregular protrusions by assembling
a branched network of actin filaments. In mammalian cells, the actin-related protein 2 and 3 (Arp2/3) complex initiates actin
assembly to form lamellipodial protrusions by binding to Wiskott-Aldrich syndrome (WASP)/WASP family verproline-homologous
protein 2 (WAVE2). In this study, colocalization of Arp2 and WAVE2 in adenocarcinoma of the lung was investigated to elucidate
its prognostic value.
Experimental Design: Immunohistochemical staining of Arp2 and WAVE2 was done on mirror sections of 115 adenocarcinomas of the lung from pathologic
stage IA to IIIA classes. Kaplan-Meier disease-free survival and overall survival curves were analyzed to determine the prognostic
significance of the coexpression of Arp2 and WAVE2.
Results: Immunoreactivity for both Arp2 and WAVE2 was detected in the same cancer cells in 78 (67.8%) of the 115 lung cancer specimens.
The proportion of cancer cells expressing both Arp2 and WAVE2 was significantly higher in cases with lymph-node metastasis
( P = 0.0046), and significantly lower in bronchioloalveolar carcinomas ( P < 0.0001). The patients whose cancer cells coexpressed them had a shorter disease-free survival time ( P < 0.0001) and overall survival time ( P < 0.0001). Multivariate Cox regression analysis revealed that coexpression of Arp2 and WAVE2 is an independent risk factor
for tumor recurrence.
Conclusions: Coexpression of Arp2 and WAVE2 is correlated with poorer patient outcome, and may be involved in the mechanism of cancer
metastasis.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>16638851</pmid><doi>10.1158/1078-0432.CCR-05-2566</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical cancer research, 2006-04, Vol.12 (8), p.2449-2454 |
| issn | 1078-0432 1557-3265 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67903632 |
| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Actin-Related Protein 2 - biosynthesis Adenocarcinoma - metabolism Adenocarcinoma - pathology Aged Antineoplastic agents Arp2/3 complex Biological and medical sciences Disease-Free Survival Female Hematologic and hematopoietic diseases Humans Hymenoptera Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunohistochemistry Immunopathology lamellipodium lung adenocarcinoma Lung Neoplasms - metabolism Lung Neoplasms - pathology Male Medical sciences metastasis Multivariate Analysis Neoplasm Staging Pharmacology. Drug treatments Platelet diseases and coagulopathies Pneumology Prognosis Survival Analysis Tumors of the respiratory system and mediastinum WAVE2 Wiskott-Aldrich Syndrome Protein Family - biosynthesis |
| title | Coexpression of Actin-Related Protein 2 and Wiskott-Aldrich Syndrome Family Verproline-Homologous Protein 2 in Adenocarcinoma of the Lung |
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