Influence of ultrasound operating parameters on ultrasound-induced thrombolysis in vitro
The effect of operating parameters on the thrombolytic potency of ultrasound (US) is important for potential therapeutic applications, but is not fully understood. Fresh human whole-blood thrombi were exposed in vitro to focused US from a diagnostic transducer driven by an impulse generator via an a...
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Veröffentlicht in: | Ultrasound in medicine & biology 2005-06, Vol.31 (6), p.841-847 |
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creator | Schäfer, Simon Kliner, Stefan Klinghammer, Lutz Kaarmann, Hans Lucic, Ivan Nixdorff, Uwe Rosenschein, Uri Daniel, Werner G. Flachskampf, Frank A. |
description | The effect of operating parameters on the thrombolytic potency of ultrasound (US) is important for potential therapeutic applications, but is not fully understood. Fresh human whole-blood thrombi were exposed
in vitro to focused US from a diagnostic transducer driven by an impulse generator
via an amplifier to vary duration (10 to 60 min), intensity (7 to 90 W/cm
2), frequency (2 to 4.5 MHz), pulsed wave duty cycle (1:5 to 1:100 and continuous wave mode) and pulse length (100 to 400 μs). Segments of thrombi (498 ± 73 mg) were submersed and insonated in saline solution. Thrombolytic efficiency was expressed as percentage loss of mass compared with controls (noninsonified thrombi). Ultrasound exposure achieved a significantly higher thrombolysis than no US, 56 ± 16 % vs. 29 ± 11 % (
n = 232,
p < 10
−6). There was an exponential saturation-type correlation with duration of insonation (r
2 = 0.64) and intensity (r
2 = 0.97), an inverse correlation with US frequency at matched intensities (r
2 = 0.76,
p < 10
−5), a logarithmic relationship with duty cycle in pulsed mode (r
2 = 0.86) and a modest direct effect of pulse length (r
2 = 0.57,
p < 10
−5). Thus, thrombolytic efficiency of US depends directly on duration, intensity, duty cycle and pulse length and inversely, on frequency. (E-mail:
frank.flachskampf@rzmail.uni-erlangen.de) |
doi_str_mv | 10.1016/j.ultrasmedbio.2005.03.005 |
format | Article |
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in vitro to focused US from a diagnostic transducer driven by an impulse generator
via an amplifier to vary duration (10 to 60 min), intensity (7 to 90 W/cm
2), frequency (2 to 4.5 MHz), pulsed wave duty cycle (1:5 to 1:100 and continuous wave mode) and pulse length (100 to 400 μs). Segments of thrombi (498 ± 73 mg) were submersed and insonated in saline solution. Thrombolytic efficiency was expressed as percentage loss of mass compared with controls (noninsonified thrombi). Ultrasound exposure achieved a significantly higher thrombolysis than no US, 56 ± 16 % vs. 29 ± 11 % (
n = 232,
p < 10
−6). There was an exponential saturation-type correlation with duration of insonation (r
2 = 0.64) and intensity (r
2 = 0.97), an inverse correlation with US frequency at matched intensities (r
2 = 0.76,
p < 10
−5), a logarithmic relationship with duty cycle in pulsed mode (r
2 = 0.86) and a modest direct effect of pulse length (r
2 = 0.57,
p < 10
−5). Thus, thrombolytic efficiency of US depends directly on duration, intensity, duty cycle and pulse length and inversely, on frequency. (E-mail:
frank.flachskampf@rzmail.uni-erlangen.de)</description><identifier>ISSN: 0301-5629</identifier><identifier>EISSN: 1879-291X</identifier><identifier>DOI: 10.1016/j.ultrasmedbio.2005.03.005</identifier><identifier>PMID: 15936499</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Case-Control Studies ; Cavitation ; Humans ; Operating parameters ; Thrombolytic Therapy - methods ; Thrombosis - therapy ; Ultrasonic Therapy - methods ; Ultrasonography, Interventional ; Ultrasound thrombolysis</subject><ispartof>Ultrasound in medicine & biology, 2005-06, Vol.31 (6), p.841-847</ispartof><rights>2005 World Federation for Ultrasound in Medicine & Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-886fcee0c5891bb3154ccaee3d3f1e3c438e513ee76208f1608964e8cd0473653</citedby><cites>FETCH-LOGICAL-c409t-886fcee0c5891bb3154ccaee3d3f1e3c438e513ee76208f1608964e8cd0473653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0301562905001237$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15936499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schäfer, Simon</creatorcontrib><creatorcontrib>Kliner, Stefan</creatorcontrib><creatorcontrib>Klinghammer, Lutz</creatorcontrib><creatorcontrib>Kaarmann, Hans</creatorcontrib><creatorcontrib>Lucic, Ivan</creatorcontrib><creatorcontrib>Nixdorff, Uwe</creatorcontrib><creatorcontrib>Rosenschein, Uri</creatorcontrib><creatorcontrib>Daniel, Werner G.</creatorcontrib><creatorcontrib>Flachskampf, Frank A.</creatorcontrib><title>Influence of ultrasound operating parameters on ultrasound-induced thrombolysis in vitro</title><title>Ultrasound in medicine & biology</title><addtitle>Ultrasound Med Biol</addtitle><description>The effect of operating parameters on the thrombolytic potency of ultrasound (US) is important for potential therapeutic applications, but is not fully understood. Fresh human whole-blood thrombi were exposed
in vitro to focused US from a diagnostic transducer driven by an impulse generator
via an amplifier to vary duration (10 to 60 min), intensity (7 to 90 W/cm
2), frequency (2 to 4.5 MHz), pulsed wave duty cycle (1:5 to 1:100 and continuous wave mode) and pulse length (100 to 400 μs). Segments of thrombi (498 ± 73 mg) were submersed and insonated in saline solution. Thrombolytic efficiency was expressed as percentage loss of mass compared with controls (noninsonified thrombi). Ultrasound exposure achieved a significantly higher thrombolysis than no US, 56 ± 16 % vs. 29 ± 11 % (
n = 232,
p < 10
−6). There was an exponential saturation-type correlation with duration of insonation (r
2 = 0.64) and intensity (r
2 = 0.97), an inverse correlation with US frequency at matched intensities (r
2 = 0.76,
p < 10
−5), a logarithmic relationship with duty cycle in pulsed mode (r
2 = 0.86) and a modest direct effect of pulse length (r
2 = 0.57,
p < 10
−5). Thus, thrombolytic efficiency of US depends directly on duration, intensity, duty cycle and pulse length and inversely, on frequency. (E-mail:
frank.flachskampf@rzmail.uni-erlangen.de)</description><subject>Case-Control Studies</subject><subject>Cavitation</subject><subject>Humans</subject><subject>Operating parameters</subject><subject>Thrombolytic Therapy - methods</subject><subject>Thrombosis - therapy</subject><subject>Ultrasonic Therapy - methods</subject><subject>Ultrasonography, Interventional</subject><subject>Ultrasound thrombolysis</subject><issn>0301-5629</issn><issn>1879-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9Lw0AUxBdRbK1-BQkevCW-zSa7WW9S_xUKXhR6W5LNi25JsnU3KfTbm5KCvelpDu8382CGkBsKEQXK79ZRX3cu9w2WhbFRDJBGwKJBTsiUZkKGsaSrUzIFBjRMeSwn5ML7NQAIzsQ5mdBUMp5IOSWrRVvVPbYaA1sFY67t2zKwG3R5Z9rPYJO7vMEOnQ9se4SEpi17jWXQfTnbFLbeeeMD0wZb0zl7Sc6qvPZ4ddAZ-Xh-ep-_hsu3l8X8YRnqBGQXZhmvNCLoNJO0KBhNE61zRFayiiLTCcswpQxR8BiyinLIJE8w0yUkgvGUzcjtmLtx9rtH36nGeI11nbdoe6-4kMBiAX-CVDAR03SfeD-C2lnvHVZq40yTu52ioPYDqLU6HkDtB1DA1CCD-frwpS-G86_10PgAPI4ADqVsDTrltdn3XxqHulOlNf_58wPZkKA_</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Schäfer, Simon</creator><creator>Kliner, Stefan</creator><creator>Klinghammer, Lutz</creator><creator>Kaarmann, Hans</creator><creator>Lucic, Ivan</creator><creator>Nixdorff, Uwe</creator><creator>Rosenschein, Uri</creator><creator>Daniel, Werner G.</creator><creator>Flachskampf, Frank A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Influence of ultrasound operating parameters on ultrasound-induced thrombolysis in vitro</title><author>Schäfer, Simon ; Kliner, Stefan ; Klinghammer, Lutz ; Kaarmann, Hans ; Lucic, Ivan ; Nixdorff, Uwe ; Rosenschein, Uri ; Daniel, Werner G. ; Flachskampf, Frank A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-886fcee0c5891bb3154ccaee3d3f1e3c438e513ee76208f1608964e8cd0473653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Case-Control Studies</topic><topic>Cavitation</topic><topic>Humans</topic><topic>Operating parameters</topic><topic>Thrombolytic Therapy - methods</topic><topic>Thrombosis - therapy</topic><topic>Ultrasonic Therapy - methods</topic><topic>Ultrasonography, Interventional</topic><topic>Ultrasound thrombolysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schäfer, Simon</creatorcontrib><creatorcontrib>Kliner, Stefan</creatorcontrib><creatorcontrib>Klinghammer, Lutz</creatorcontrib><creatorcontrib>Kaarmann, Hans</creatorcontrib><creatorcontrib>Lucic, Ivan</creatorcontrib><creatorcontrib>Nixdorff, Uwe</creatorcontrib><creatorcontrib>Rosenschein, Uri</creatorcontrib><creatorcontrib>Daniel, Werner G.</creatorcontrib><creatorcontrib>Flachskampf, Frank A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Ultrasound in medicine & biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schäfer, Simon</au><au>Kliner, Stefan</au><au>Klinghammer, Lutz</au><au>Kaarmann, Hans</au><au>Lucic, Ivan</au><au>Nixdorff, Uwe</au><au>Rosenschein, Uri</au><au>Daniel, Werner G.</au><au>Flachskampf, Frank A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of ultrasound operating parameters on ultrasound-induced thrombolysis in vitro</atitle><jtitle>Ultrasound in medicine & biology</jtitle><addtitle>Ultrasound Med Biol</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>31</volume><issue>6</issue><spage>841</spage><epage>847</epage><pages>841-847</pages><issn>0301-5629</issn><eissn>1879-291X</eissn><abstract>The effect of operating parameters on the thrombolytic potency of ultrasound (US) is important for potential therapeutic applications, but is not fully understood. Fresh human whole-blood thrombi were exposed
in vitro to focused US from a diagnostic transducer driven by an impulse generator
via an amplifier to vary duration (10 to 60 min), intensity (7 to 90 W/cm
2), frequency (2 to 4.5 MHz), pulsed wave duty cycle (1:5 to 1:100 and continuous wave mode) and pulse length (100 to 400 μs). Segments of thrombi (498 ± 73 mg) were submersed and insonated in saline solution. Thrombolytic efficiency was expressed as percentage loss of mass compared with controls (noninsonified thrombi). Ultrasound exposure achieved a significantly higher thrombolysis than no US, 56 ± 16 % vs. 29 ± 11 % (
n = 232,
p < 10
−6). There was an exponential saturation-type correlation with duration of insonation (r
2 = 0.64) and intensity (r
2 = 0.97), an inverse correlation with US frequency at matched intensities (r
2 = 0.76,
p < 10
−5), a logarithmic relationship with duty cycle in pulsed mode (r
2 = 0.86) and a modest direct effect of pulse length (r
2 = 0.57,
p < 10
−5). Thus, thrombolytic efficiency of US depends directly on duration, intensity, duty cycle and pulse length and inversely, on frequency. (E-mail:
frank.flachskampf@rzmail.uni-erlangen.de)</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>15936499</pmid><doi>10.1016/j.ultrasmedbio.2005.03.005</doi><tpages>7</tpages></addata></record> |
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subjects | Case-Control Studies Cavitation Humans Operating parameters Thrombolytic Therapy - methods Thrombosis - therapy Ultrasonic Therapy - methods Ultrasonography, Interventional Ultrasound thrombolysis |
title | Influence of ultrasound operating parameters on ultrasound-induced thrombolysis in vitro |
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