Sensitivity to chilling of medaka ( Oryzias latipes) embryos at various developmental stages

As an essential step toward cryopreservation of fish embryos, we examined the chilling sensitivity of medaka ( Oryzias latipes) embryos at various developmental stages. Embryos at the 2–4 cell, 8–16 cell, morula, blastula, and early gastrula stages were suspended in Hanks solution. They were chilled...

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Veröffentlicht in:	Theriogenology 2005-07, Vol.64 (1), p.112-122
Hauptverfasser:	Valdez, Delgado M., Miyamoto, Akira, Hara, Takao, Edashige, Keisuke, Kasai, Magosaburo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chilling injury Cold Temperature Developmental stage Embryo Embryo Culture Techniques - veterinary embryogenesis Embryonic Development embryonic mortality Gastrula Medaka Oryzias - embryology Refrigeration Time Factors Tissue Preservation - veterinary
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