Effect of human chorionic gonadotrophin supplementation during different culture periods on in vitro maturation of canine oocytes

The IVM of canine oocytes is characterized by low rates of metaphase II. The objective of this study was to evaluate the effects of hCG on meiotic development of canine oocytes for culture periods up to 96 h. Oocytes were collected after ovariohysterectomy. Only oocytes >110 μm in diameter, with...

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Veröffentlicht in:	Theriogenology 2005-07, Vol.64 (1), p.1-11
Hauptverfasser:	De los Reyes, Monica, Lange, Johanna de, Miranda, Pedro, Palominos, Jaime, Barros, Claudio
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Canine Cell Culture Techniques Chorionic Gonadotropin - administration & dosage Culture Media cumulus oophorus Dogs duration Female Fluorescent Dyes hCG Humans In vitro maturation Meiosis Microscopy, Fluorescence Oocytes Oocytes - cytology Ovarian Follicle - cytology Propidium
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