Birth of mice after in vitro fertilization using C57BL/6 sperm transported within epididymides at refrigerated temperatures

The transportation of cryopreserved spermatozoa is an economical, efficient, and safe method for the distribution of mouse strains from one facility to another. However, spermatozoa from some strains, including C57BL/6 (B6), are very sensitive to freezing and thawing and frequently fail to fertilize...

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Veröffentlicht in:	Theriogenology 2005-07, Vol.64 (1), p.135-143
Hauptverfasser:	Mochida, K., Ohkawa, M., Inoue, K., Valdez, D.M., Kasai, M., Ogura, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	animal genetic resources Animals Cold Temperature Cryopreservation Embryo Culture Techniques - methods Embryo Culture Techniques - veterinary Embryo Transfer - veterinary Epididymis Epididymis - cytology Female Fertilization in Vitro - veterinary IVF laboratory animals Male Mice Mice, Inbred C57BL Mouse Pregnancy Semen Preservation - methods Semen Preservation - veterinary Specimen Handling - methods Specimen Handling - veterinary Spermatozoa storage temperature strain differences strains Transportation
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