Gene transfer using the mature form of VEGF-D reduces neointimal thickening through nitric oxide-dependent mechanism
Gene transfer to the vessel wall using vascular endothelial growth factors (VEGFs) has shown therapeutic potential for the treatment of restenosis. In this study, we evaluated the effect of catheter-mediated adenoviral (Ad) gene transfer of the mature form of VEGF-D (VEGF-D ΔNΔC ) in balloon-denuded...
Gespeichert in:
| Veröffentlicht in: | Gene therapy 2005-06, Vol.12 (12), p.980-987 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 987 |
|---|---|
| container_issue | 12 |
| container_start_page | 980 |
| container_title | Gene therapy |
| container_volume | 12 |
| creator | Rutanen, J Turunen, A-M Teittinen, M Rissanen, T T Heikura, T Koponen, J K Gruchala, M Inkala, M Jauhiainen, S Hiltunen, M O Turunen, M P Stacker, S A Achen, M G Ylä-Herttuala, S |
| description | Gene transfer to the vessel wall using vascular endothelial growth factors (VEGFs) has shown therapeutic potential for the treatment of restenosis. In this study, we evaluated the effect of catheter-mediated adenoviral (Ad) gene transfer of the mature form of VEGF-D (VEGF-D
ΔNΔC
) in balloon-denuded cholesterol-fed rabbit aorta. AdLacZ was used as a control. Transduced VEGF-D
ΔNΔC
mRNA was detectable in the arterial wall with RT-PCR at 6, 14 and 28 days. Gene transfer efficiency as detected with X-gal staining 6 days after the AdLacZ transduction was 1.91±1.32% in intima. AdVEGF-D
ΔNΔC
gene transfer led to 52% reduction in intima/media ratio (I/M) as compared to the AdLacZ controls at 14 days time point. At 6 days there were no differences in I/M, but the number of macrophages in the vessel wall was 85% lower in the AdVEGF-D
ΔNΔC
group as compared to the controls. The therapeutic effect was no longer detectable 28 days after the gene transfer. The therapeutic effect of VEGF-D
ΔNΔC
was nitric oxide (NO)-dependent as the feeding of NO synthase inhibitor,
L
-NAME, blocked the reduction in intimal thickening. It is concluded that AdVEGF-D
ΔNΔC
gene transfer reduces intimal thickening and macrophage influx into the vessel wall in balloon-denuded rabbit aortas. |
| doi_str_mv | 10.1038/sj.gt.3302489 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_67899256</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A182812014</galeid><sourcerecordid>A182812014</sourcerecordid><originalsourceid>FETCH-LOGICAL-c617t-e1519defee2c7b396e2bfe58489607f1174be6577e443459e9ee1e9db6c83d0f3</originalsourceid><addsrcrecordid>eNqF0suL1DAYAPAiijuuHr1KUFzw0DFp0zyOy7o7LiwIvq6hk37pZGyTMUlh_e_NMIVxZEVyKKS_L8n3KIqXBC8JrsX7uF32aVnXuKJCPioWhHJWNpRVj4sFlkyWnFTirHgW4xZjTLmonhZnpOGNxEQsirQCByiF1kUDAU3Ruh6lDaCxTVMAZHwYkTfo-_XqpvyAAnSThogceOuSHdshY6t_gDvEBT_1G-RsClYjf287KDvYgevAJTSC3rTOxvF58cS0Q4QX8_e8-HZz_fXqY3n3aXV7dXlXakZ4KoE0RHZgACrN17VkUK0NNCInyjA3hHC6BtZwDpTWtJEgAQjIbs20qDts6vPi4nDuLvifE8SkRhs1DEOb3z9FxbiQsmrYfyHhDZWU1Rm--Qtu_RRcTkJVjFJGcxf26vU_FRGMZ9gcj-rbAZR1xucu6P296pKISpAKE5rV8gGVVwej1d6BsXn_JODdSUA2Ce5T304xqtsvn0_txR92A-2QNtEPU7LexVNYHqAOPsYARu1Cbn74pQhW-zFUcav6pOYxzP7VXIBpPUJ31PPcZfB2Bm3U7WDy_Gkbj44Jvq_UMf2Yf7kewrGSD9_8G2fA8Y8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>218674465</pqid></control><display><type>article</type><title>Gene transfer using the mature form of VEGF-D reduces neointimal thickening through nitric oxide-dependent mechanism</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>SpringerLink Journals - AutoHoldings</source><creator>Rutanen, J ; Turunen, A-M ; Teittinen, M ; Rissanen, T T ; Heikura, T ; Koponen, J K ; Gruchala, M ; Inkala, M ; Jauhiainen, S ; Hiltunen, M O ; Turunen, M P ; Stacker, S A ; Achen, M G ; Ylä-Herttuala, S</creator><creatorcontrib>Rutanen, J ; Turunen, A-M ; Teittinen, M ; Rissanen, T T ; Heikura, T ; Koponen, J K ; Gruchala, M ; Inkala, M ; Jauhiainen, S ; Hiltunen, M O ; Turunen, M P ; Stacker, S A ; Achen, M G ; Ylä-Herttuala, S</creatorcontrib><description>Gene transfer to the vessel wall using vascular endothelial growth factors (VEGFs) has shown therapeutic potential for the treatment of restenosis. In this study, we evaluated the effect of catheter-mediated adenoviral (Ad) gene transfer of the mature form of VEGF-D (VEGF-D
ΔNΔC
) in balloon-denuded cholesterol-fed rabbit aorta. AdLacZ was used as a control. Transduced VEGF-D
ΔNΔC
mRNA was detectable in the arterial wall with RT-PCR at 6, 14 and 28 days. Gene transfer efficiency as detected with X-gal staining 6 days after the AdLacZ transduction was 1.91±1.32% in intima. AdVEGF-D
ΔNΔC
gene transfer led to 52% reduction in intima/media ratio (I/M) as compared to the AdLacZ controls at 14 days time point. At 6 days there were no differences in I/M, but the number of macrophages in the vessel wall was 85% lower in the AdVEGF-D
ΔNΔC
group as compared to the controls. The therapeutic effect was no longer detectable 28 days after the gene transfer. The therapeutic effect of VEGF-D
ΔNΔC
was nitric oxide (NO)-dependent as the feeding of NO synthase inhibitor,
L
-NAME, blocked the reduction in intimal thickening. It is concluded that AdVEGF-D
ΔNΔC
gene transfer reduces intimal thickening and macrophage influx into the vessel wall in balloon-denuded rabbit aortas.</description><identifier>ISSN: 0969-7128</identifier><identifier>EISSN: 1476-5462</identifier><identifier>DOI: 10.1038/sj.gt.3302489</identifier><identifier>PMID: 15759018</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Adenoviridae - genetics ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Aorta ; Aortic Diseases - metabolism ; Aortic Diseases - pathology ; Aortic Diseases - therapy ; Applied cell therapy and gene therapy ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Biotechnology ; Care and treatment ; Catheterization ; Catheters ; Cell Biology ; Cholesterol ; Constriction, Pathologic - therapy ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Therapy ; Gene transfer ; Genetic Therapy - methods ; Genetic Vectors - administration & dosage ; Growth factors ; Health aspects ; Health. Pharmaceutical industry ; Human Genetics ; Industrial applications and implications. Economical aspects ; Macrophages ; Medical sciences ; mRNA ; Nanotechnology ; Neovascularization, Pathologic ; NG-Nitroarginine methyl ester ; Nitric oxide ; Nitric Oxide - metabolism ; Nitric-oxide synthase ; Physiological aspects ; Polymerase chain reaction ; Rabbits ; Recurrence ; research-article ; Restenosis ; Reverse Transcriptase Polymerase Chain Reaction ; Risk factors ; Stenosis ; Transduction, Genetic - methods ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Tunica Intima - metabolism ; Tunica Intima - pathology ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor D - genetics ; Vascular Endothelial Growth Factor D - metabolism</subject><ispartof>Gene therapy, 2005-06, Vol.12 (12), p.980-987</ispartof><rights>Springer Nature Limited 2005</rights><rights>2005 INIST-CNRS</rights><rights>COPYRIGHT 2005 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jun 2005</rights><rights>Nature Publishing Group 2005.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c617t-e1519defee2c7b396e2bfe58489607f1174be6577e443459e9ee1e9db6c83d0f3</citedby><cites>FETCH-LOGICAL-c617t-e1519defee2c7b396e2bfe58489607f1174be6577e443459e9ee1e9db6c83d0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/sj.gt.3302489$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/sj.gt.3302489$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16872186$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15759018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rutanen, J</creatorcontrib><creatorcontrib>Turunen, A-M</creatorcontrib><creatorcontrib>Teittinen, M</creatorcontrib><creatorcontrib>Rissanen, T T</creatorcontrib><creatorcontrib>Heikura, T</creatorcontrib><creatorcontrib>Koponen, J K</creatorcontrib><creatorcontrib>Gruchala, M</creatorcontrib><creatorcontrib>Inkala, M</creatorcontrib><creatorcontrib>Jauhiainen, S</creatorcontrib><creatorcontrib>Hiltunen, M O</creatorcontrib><creatorcontrib>Turunen, M P</creatorcontrib><creatorcontrib>Stacker, S A</creatorcontrib><creatorcontrib>Achen, M G</creatorcontrib><creatorcontrib>Ylä-Herttuala, S</creatorcontrib><title>Gene transfer using the mature form of VEGF-D reduces neointimal thickening through nitric oxide-dependent mechanism</title><title>Gene therapy</title><addtitle>Gene Ther</addtitle><addtitle>Gene Ther</addtitle><description>Gene transfer to the vessel wall using vascular endothelial growth factors (VEGFs) has shown therapeutic potential for the treatment of restenosis. In this study, we evaluated the effect of catheter-mediated adenoviral (Ad) gene transfer of the mature form of VEGF-D (VEGF-D
ΔNΔC
) in balloon-denuded cholesterol-fed rabbit aorta. AdLacZ was used as a control. Transduced VEGF-D
ΔNΔC
mRNA was detectable in the arterial wall with RT-PCR at 6, 14 and 28 days. Gene transfer efficiency as detected with X-gal staining 6 days after the AdLacZ transduction was 1.91±1.32% in intima. AdVEGF-D
ΔNΔC
gene transfer led to 52% reduction in intima/media ratio (I/M) as compared to the AdLacZ controls at 14 days time point. At 6 days there were no differences in I/M, but the number of macrophages in the vessel wall was 85% lower in the AdVEGF-D
ΔNΔC
group as compared to the controls. The therapeutic effect was no longer detectable 28 days after the gene transfer. The therapeutic effect of VEGF-D
ΔNΔC
was nitric oxide (NO)-dependent as the feeding of NO synthase inhibitor,
L
-NAME, blocked the reduction in intimal thickening. It is concluded that AdVEGF-D
ΔNΔC
gene transfer reduces intimal thickening and macrophage influx into the vessel wall in balloon-denuded rabbit aortas.</description><subject>Adenoviridae - genetics</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Aorta</subject><subject>Aortic Diseases - metabolism</subject><subject>Aortic Diseases - pathology</subject><subject>Aortic Diseases - therapy</subject><subject>Applied cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Care and treatment</subject><subject>Catheterization</subject><subject>Catheters</subject><subject>Cell Biology</subject><subject>Cholesterol</subject><subject>Constriction, Pathologic - therapy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Therapy</subject><subject>Gene transfer</subject><subject>Genetic Therapy - methods</subject><subject>Genetic Vectors - administration & dosage</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Health. Pharmaceutical industry</subject><subject>Human Genetics</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Macrophages</subject><subject>Medical sciences</subject><subject>mRNA</subject><subject>Nanotechnology</subject><subject>Neovascularization, Pathologic</subject><subject>NG-Nitroarginine methyl ester</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitric-oxide synthase</subject><subject>Physiological aspects</subject><subject>Polymerase chain reaction</subject><subject>Rabbits</subject><subject>Recurrence</subject><subject>research-article</subject><subject>Restenosis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Risk factors</subject><subject>Stenosis</subject><subject>Transduction, Genetic - methods</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><subject>Tunica Intima - metabolism</subject><subject>Tunica Intima - pathology</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor D - genetics</subject><subject>Vascular Endothelial Growth Factor D - metabolism</subject><issn>0969-7128</issn><issn>1476-5462</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0suL1DAYAPAiijuuHr1KUFzw0DFp0zyOy7o7LiwIvq6hk37pZGyTMUlh_e_NMIVxZEVyKKS_L8n3KIqXBC8JrsX7uF32aVnXuKJCPioWhHJWNpRVj4sFlkyWnFTirHgW4xZjTLmonhZnpOGNxEQsirQCByiF1kUDAU3Ruh6lDaCxTVMAZHwYkTfo-_XqpvyAAnSThogceOuSHdshY6t_gDvEBT_1G-RsClYjf287KDvYgevAJTSC3rTOxvF58cS0Q4QX8_e8-HZz_fXqY3n3aXV7dXlXakZ4KoE0RHZgACrN17VkUK0NNCInyjA3hHC6BtZwDpTWtJEgAQjIbs20qDts6vPi4nDuLvifE8SkRhs1DEOb3z9FxbiQsmrYfyHhDZWU1Rm--Qtu_RRcTkJVjFJGcxf26vU_FRGMZ9gcj-rbAZR1xucu6P296pKISpAKE5rV8gGVVwej1d6BsXn_JODdSUA2Ce5T304xqtsvn0_txR92A-2QNtEPU7LexVNYHqAOPsYARu1Cbn74pQhW-zFUcav6pOYxzP7VXIBpPUJ31PPcZfB2Bm3U7WDy_Gkbj44Jvq_UMf2Yf7kewrGSD9_8G2fA8Y8</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Rutanen, J</creator><creator>Turunen, A-M</creator><creator>Teittinen, M</creator><creator>Rissanen, T T</creator><creator>Heikura, T</creator><creator>Koponen, J K</creator><creator>Gruchala, M</creator><creator>Inkala, M</creator><creator>Jauhiainen, S</creator><creator>Hiltunen, M O</creator><creator>Turunen, M P</creator><creator>Stacker, S A</creator><creator>Achen, M G</creator><creator>Ylä-Herttuala, S</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Gene transfer using the mature form of VEGF-D reduces neointimal thickening through nitric oxide-dependent mechanism</title><author>Rutanen, J ; Turunen, A-M ; Teittinen, M ; Rissanen, T T ; Heikura, T ; Koponen, J K ; Gruchala, M ; Inkala, M ; Jauhiainen, S ; Hiltunen, M O ; Turunen, M P ; Stacker, S A ; Achen, M G ; Ylä-Herttuala, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c617t-e1519defee2c7b396e2bfe58489607f1174be6577e443459e9ee1e9db6c83d0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenoviridae - genetics</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Aorta</topic><topic>Aortic Diseases - metabolism</topic><topic>Aortic Diseases - pathology</topic><topic>Aortic Diseases - therapy</topic><topic>Applied cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Care and treatment</topic><topic>Catheterization</topic><topic>Catheters</topic><topic>Cell Biology</topic><topic>Cholesterol</topic><topic>Constriction, Pathologic - therapy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Therapy</topic><topic>Gene transfer</topic><topic>Genetic Therapy - methods</topic><topic>Genetic Vectors - administration & dosage</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>Health. Pharmaceutical industry</topic><topic>Human Genetics</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Macrophages</topic><topic>Medical sciences</topic><topic>mRNA</topic><topic>Nanotechnology</topic><topic>Neovascularization, Pathologic</topic><topic>NG-Nitroarginine methyl ester</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitric-oxide synthase</topic><topic>Physiological aspects</topic><topic>Polymerase chain reaction</topic><topic>Rabbits</topic><topic>Recurrence</topic><topic>research-article</topic><topic>Restenosis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Risk factors</topic><topic>Stenosis</topic><topic>Transduction, Genetic - methods</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><topic>Tunica Intima - metabolism</topic><topic>Tunica Intima - pathology</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor D - genetics</topic><topic>Vascular Endothelial Growth Factor D - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rutanen, J</creatorcontrib><creatorcontrib>Turunen, A-M</creatorcontrib><creatorcontrib>Teittinen, M</creatorcontrib><creatorcontrib>Rissanen, T T</creatorcontrib><creatorcontrib>Heikura, T</creatorcontrib><creatorcontrib>Koponen, J K</creatorcontrib><creatorcontrib>Gruchala, M</creatorcontrib><creatorcontrib>Inkala, M</creatorcontrib><creatorcontrib>Jauhiainen, S</creatorcontrib><creatorcontrib>Hiltunen, M O</creatorcontrib><creatorcontrib>Turunen, M P</creatorcontrib><creatorcontrib>Stacker, S A</creatorcontrib><creatorcontrib>Achen, M G</creatorcontrib><creatorcontrib>Ylä-Herttuala, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rutanen, J</au><au>Turunen, A-M</au><au>Teittinen, M</au><au>Rissanen, T T</au><au>Heikura, T</au><au>Koponen, J K</au><au>Gruchala, M</au><au>Inkala, M</au><au>Jauhiainen, S</au><au>Hiltunen, M O</au><au>Turunen, M P</au><au>Stacker, S A</au><au>Achen, M G</au><au>Ylä-Herttuala, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene transfer using the mature form of VEGF-D reduces neointimal thickening through nitric oxide-dependent mechanism</atitle><jtitle>Gene therapy</jtitle><stitle>Gene Ther</stitle><addtitle>Gene Ther</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>12</volume><issue>12</issue><spage>980</spage><epage>987</epage><pages>980-987</pages><issn>0969-7128</issn><eissn>1476-5462</eissn><abstract>Gene transfer to the vessel wall using vascular endothelial growth factors (VEGFs) has shown therapeutic potential for the treatment of restenosis. In this study, we evaluated the effect of catheter-mediated adenoviral (Ad) gene transfer of the mature form of VEGF-D (VEGF-D
ΔNΔC
) in balloon-denuded cholesterol-fed rabbit aorta. AdLacZ was used as a control. Transduced VEGF-D
ΔNΔC
mRNA was detectable in the arterial wall with RT-PCR at 6, 14 and 28 days. Gene transfer efficiency as detected with X-gal staining 6 days after the AdLacZ transduction was 1.91±1.32% in intima. AdVEGF-D
ΔNΔC
gene transfer led to 52% reduction in intima/media ratio (I/M) as compared to the AdLacZ controls at 14 days time point. At 6 days there were no differences in I/M, but the number of macrophages in the vessel wall was 85% lower in the AdVEGF-D
ΔNΔC
group as compared to the controls. The therapeutic effect was no longer detectable 28 days after the gene transfer. The therapeutic effect of VEGF-D
ΔNΔC
was nitric oxide (NO)-dependent as the feeding of NO synthase inhibitor,
L
-NAME, blocked the reduction in intimal thickening. It is concluded that AdVEGF-D
ΔNΔC
gene transfer reduces intimal thickening and macrophage influx into the vessel wall in balloon-denuded rabbit aortas.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>15759018</pmid><doi>10.1038/sj.gt.3302489</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0969-7128 |
| ispartof | Gene therapy, 2005-06, Vol.12 (12), p.980-987 |
| issn | 0969-7128 1476-5462 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67899256 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; SpringerLink Journals - AutoHoldings |
| subjects | Adenoviridae - genetics Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Aorta Aortic Diseases - metabolism Aortic Diseases - pathology Aortic Diseases - therapy Applied cell therapy and gene therapy Biological and medical sciences Biomedical and Life Sciences Biomedicine Biotechnology Care and treatment Catheterization Catheters Cell Biology Cholesterol Constriction, Pathologic - therapy Fundamental and applied biological sciences. Psychology Gene Expression Gene Therapy Gene transfer Genetic Therapy - methods Genetic Vectors - administration & dosage Growth factors Health aspects Health. Pharmaceutical industry Human Genetics Industrial applications and implications. Economical aspects Macrophages Medical sciences mRNA Nanotechnology Neovascularization, Pathologic NG-Nitroarginine methyl ester Nitric oxide Nitric Oxide - metabolism Nitric-oxide synthase Physiological aspects Polymerase chain reaction Rabbits Recurrence research-article Restenosis Reverse Transcriptase Polymerase Chain Reaction Risk factors Stenosis Transduction, Genetic - methods Transfusions. Complications. Transfusion reactions. Cell and gene therapy Tunica Intima - metabolism Tunica Intima - pathology Vascular endothelial growth factor Vascular Endothelial Growth Factor D - genetics Vascular Endothelial Growth Factor D - metabolism |
| title | Gene transfer using the mature form of VEGF-D reduces neointimal thickening through nitric oxide-dependent mechanism |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T12%3A45%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Gene%20transfer%20using%20the%20mature%20form%20of%20VEGF-D%20reduces%20neointimal%20thickening%20through%20nitric%20oxide-dependent%20mechanism&rft.jtitle=Gene%20therapy&rft.au=Rutanen,%20J&rft.date=2005-06-01&rft.volume=12&rft.issue=12&rft.spage=980&rft.epage=987&rft.pages=980-987&rft.issn=0969-7128&rft.eissn=1476-5462&rft_id=info:doi/10.1038/sj.gt.3302489&rft_dat=%3Cgale_proqu%3EA182812014%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=218674465&rft_id=info:pmid/15759018&rft_galeid=A182812014&rfr_iscdi=true |