Expression Profile and Clonality of T-Cell Receptor Beta Variable Genes in Normal Human Endometrium
Problem In spite of their key immunological role, αβ+ T cells residing in endometrium have not been extensively explored. We analyzed here expression profile of TCRBV genes in normal human endometrium compared with peripheral blood. Methods Samples were taken from normal reproductive women. RT‐PCR u...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2006-05, Vol.55 (5), p.349-359 |
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| container_title | American journal of reproductive immunology (1989) |
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| creator | Dokouhaki, Pouneh Moghadam, Rosa Akbariasbagh, Firoozeh Zarnani, Amirhassan Novin, Marefat Ghaffari Razavi, Alireza Jeddi-Tehrani, Mahmood |
| description | Problem
In spite of their key immunological role, αβ+ T cells residing in endometrium have not been extensively explored. We analyzed here expression profile of TCRBV genes in normal human endometrium compared with peripheral blood.
Methods
Samples were taken from normal reproductive women. RT‐PCR using BV‐gene specific primers was performed on blood and endometrial samples. After blotting, hybridization with radiolabelled probe and autoradiography, relative expression of each TCRBV family was determined. Clonal expansions of the over‐expressed genes were assessed by CDR3 length polymorphism.
Results
Only one gene (TCRBV7) was expressed significantly and two other genes marginally more in the endometrium compared with blood. All three TCRBV genes examined showed a rather restricted pattern in the endometrium in contrast to polyclonal patterns in the blood.
Conclusion
Our results stress the similarities between T cells residing in different mucosal tissues and provide a basis for future investigations about endometrial T cells and their antigen specificities in gynecological problems. |
| doi_str_mv | 10.1111/j.1600-0897.2006.00375.x |
| format | Article |
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In spite of their key immunological role, αβ+ T cells residing in endometrium have not been extensively explored. We analyzed here expression profile of TCRBV genes in normal human endometrium compared with peripheral blood.
Methods
Samples were taken from normal reproductive women. RT‐PCR using BV‐gene specific primers was performed on blood and endometrial samples. After blotting, hybridization with radiolabelled probe and autoradiography, relative expression of each TCRBV family was determined. Clonal expansions of the over‐expressed genes were assessed by CDR3 length polymorphism.
Results
Only one gene (TCRBV7) was expressed significantly and two other genes marginally more in the endometrium compared with blood. All three TCRBV genes examined showed a rather restricted pattern in the endometrium in contrast to polyclonal patterns in the blood.
Conclusion
Our results stress the similarities between T cells residing in different mucosal tissues and provide a basis for future investigations about endometrial T cells and their antigen specificities in gynecological problems.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.1600-0897.2006.00375.x</identifier><identifier>PMID: 16635209</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Base Sequence ; DNA, Complementary - genetics ; Endometrium ; Endometrium - cytology ; Endometrium - immunology ; Female ; Gene Expression Profiling ; Genes, T-Cell Receptor beta ; Humans ; immunology ; Polymorphism, Genetic ; T cell receptor ; T-cell ; T-Lymphocytes - immunology ; TCRBV</subject><ispartof>American journal of reproductive immunology (1989), 2006-05, Vol.55 (5), p.349-359</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4355-18e43d1bd18e2002f47d485164bfc48bb650906f2f80f3d84d7986694434db183</citedby><cites>FETCH-LOGICAL-c4355-18e43d1bd18e2002f47d485164bfc48bb650906f2f80f3d84d7986694434db183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0897.2006.00375.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0897.2006.00375.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16635209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dokouhaki, Pouneh</creatorcontrib><creatorcontrib>Moghadam, Rosa</creatorcontrib><creatorcontrib>Akbariasbagh, Firoozeh</creatorcontrib><creatorcontrib>Zarnani, Amirhassan</creatorcontrib><creatorcontrib>Novin, Marefat Ghaffari</creatorcontrib><creatorcontrib>Razavi, Alireza</creatorcontrib><creatorcontrib>Jeddi-Tehrani, Mahmood</creatorcontrib><title>Expression Profile and Clonality of T-Cell Receptor Beta Variable Genes in Normal Human Endometrium</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem
In spite of their key immunological role, αβ+ T cells residing in endometrium have not been extensively explored. We analyzed here expression profile of TCRBV genes in normal human endometrium compared with peripheral blood.
Methods
Samples were taken from normal reproductive women. RT‐PCR using BV‐gene specific primers was performed on blood and endometrial samples. After blotting, hybridization with radiolabelled probe and autoradiography, relative expression of each TCRBV family was determined. Clonal expansions of the over‐expressed genes were assessed by CDR3 length polymorphism.
Results
Only one gene (TCRBV7) was expressed significantly and two other genes marginally more in the endometrium compared with blood. All three TCRBV genes examined showed a rather restricted pattern in the endometrium in contrast to polyclonal patterns in the blood.
Conclusion
Our results stress the similarities between T cells residing in different mucosal tissues and provide a basis for future investigations about endometrial T cells and their antigen specificities in gynecological problems.</description><subject>Adult</subject><subject>Base Sequence</subject><subject>DNA, Complementary - genetics</subject><subject>Endometrium</subject><subject>Endometrium - cytology</subject><subject>Endometrium - immunology</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Genes, T-Cell Receptor beta</subject><subject>Humans</subject><subject>immunology</subject><subject>Polymorphism, Genetic</subject><subject>T cell receptor</subject><subject>T-cell</subject><subject>T-Lymphocytes - immunology</subject><subject>TCRBV</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv3CAUhVHVqkmT_IWKVXd2L-ZhLHWTjuaRKkofymOJsAHJU9tMwVZm_n1xZ5QuWzZcwXcu3HMQwgRyktbHbU4EQAayKvMCQOQAtOT5_hU6f7l4nWpgIisZyDP0LsYtQDqn5Vt0RoSgvIDqHDXL_S7YGFs_4G_Bu7azWA8GLzo_6K4dD9g7fJ8tbNfhH7axu9EH_NmOGj_q0Oo64Ws72IjbAd_50OsOb6ZeD3g5GN_bMbRTf4neON1Fe3XaL9DDanm_2GS3X9c3i-vbrGGU84xIy6ghtUlFGqpwrDRMciJY7Rom61pwqEC4wklw1EhmykoKUTFGmamJpBfow7HvLvhfk42j6tvYpJ_rwfopKlEmWzhn_wRJSSThokigPIJN8DEG69QutL0OB0VAzUmorZoNV7Phak5C_UlC7ZP0_emNqe6t-Ss8WZ-AT0fgOXl--O_G6vrLTSqSPDvK2zja_Ytch59pzpl8ulsrWH1fFcXTRq3ob_Y2pHo</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Dokouhaki, Pouneh</creator><creator>Moghadam, Rosa</creator><creator>Akbariasbagh, Firoozeh</creator><creator>Zarnani, Amirhassan</creator><creator>Novin, Marefat Ghaffari</creator><creator>Razavi, Alireza</creator><creator>Jeddi-Tehrani, Mahmood</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200605</creationdate><title>Expression Profile and Clonality of T-Cell Receptor Beta Variable Genes in Normal Human Endometrium</title><author>Dokouhaki, Pouneh ; Moghadam, Rosa ; Akbariasbagh, Firoozeh ; Zarnani, Amirhassan ; Novin, Marefat Ghaffari ; Razavi, Alireza ; Jeddi-Tehrani, Mahmood</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4355-18e43d1bd18e2002f47d485164bfc48bb650906f2f80f3d84d7986694434db183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Base Sequence</topic><topic>DNA, Complementary - genetics</topic><topic>Endometrium</topic><topic>Endometrium - cytology</topic><topic>Endometrium - immunology</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Genes, T-Cell Receptor beta</topic><topic>Humans</topic><topic>immunology</topic><topic>Polymorphism, Genetic</topic><topic>T cell receptor</topic><topic>T-cell</topic><topic>T-Lymphocytes - immunology</topic><topic>TCRBV</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dokouhaki, Pouneh</creatorcontrib><creatorcontrib>Moghadam, Rosa</creatorcontrib><creatorcontrib>Akbariasbagh, Firoozeh</creatorcontrib><creatorcontrib>Zarnani, Amirhassan</creatorcontrib><creatorcontrib>Novin, Marefat Ghaffari</creatorcontrib><creatorcontrib>Razavi, Alireza</creatorcontrib><creatorcontrib>Jeddi-Tehrani, Mahmood</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dokouhaki, Pouneh</au><au>Moghadam, Rosa</au><au>Akbariasbagh, Firoozeh</au><au>Zarnani, Amirhassan</au><au>Novin, Marefat Ghaffari</au><au>Razavi, Alireza</au><au>Jeddi-Tehrani, Mahmood</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression Profile and Clonality of T-Cell Receptor Beta Variable Genes in Normal Human Endometrium</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2006-05</date><risdate>2006</risdate><volume>55</volume><issue>5</issue><spage>349</spage><epage>359</epage><pages>349-359</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem
In spite of their key immunological role, αβ+ T cells residing in endometrium have not been extensively explored. We analyzed here expression profile of TCRBV genes in normal human endometrium compared with peripheral blood.
Methods
Samples were taken from normal reproductive women. RT‐PCR using BV‐gene specific primers was performed on blood and endometrial samples. After blotting, hybridization with radiolabelled probe and autoradiography, relative expression of each TCRBV family was determined. Clonal expansions of the over‐expressed genes were assessed by CDR3 length polymorphism.
Results
Only one gene (TCRBV7) was expressed significantly and two other genes marginally more in the endometrium compared with blood. All three TCRBV genes examined showed a rather restricted pattern in the endometrium in contrast to polyclonal patterns in the blood.
Conclusion
Our results stress the similarities between T cells residing in different mucosal tissues and provide a basis for future investigations about endometrial T cells and their antigen specificities in gynecological problems.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16635209</pmid><doi>10.1111/j.1600-0897.2006.00375.x</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1046-7408 |
| ispartof | American journal of reproductive immunology (1989), 2006-05, Vol.55 (5), p.349-359 |
| issn | 1046-7408 1600-0897 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67897554 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adult Base Sequence DNA, Complementary - genetics Endometrium Endometrium - cytology Endometrium - immunology Female Gene Expression Profiling Genes, T-Cell Receptor beta Humans immunology Polymorphism, Genetic T cell receptor T-cell T-Lymphocytes - immunology TCRBV |
| title | Expression Profile and Clonality of T-Cell Receptor Beta Variable Genes in Normal Human Endometrium |
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