Comparison of Vaginal Cytokine Collection Methods
Objective The objective of our study was to correlate the interleukin‐6 (IL‐6) concentrations detected in patient‐collected specimens with provider‐collected specimens and compare the reproducibility of the methods. Study design All enrolled participants underwent pelvic examination with collection...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2006-05, Vol.55 (5), p.315-320 |
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| container_title | American journal of reproductive immunology (1989) |
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| creator | Faro, Constance J. Hollier, Lisa M. Bishop, Karen |
| description | Objective
The objective of our study was to correlate the interleukin‐6 (IL‐6) concentrations detected in patient‐collected specimens with provider‐collected specimens and compare the reproducibility of the methods.
Study design
All enrolled participants underwent pelvic examination with collection of cytokine samples by the provider and also collected samples themselves using vaginal swabs. The order of sample collection was randomly assigned. All samples were frozen at −80°C for batch analysis. A commercial enzyme‐linked immunosorbent assay was used to determine the concentrations of IL‐6 in all samples.
Results
IL‐6 concentrations from wicks and swabs were correlated in a linear fashion (r = 0.67, P |
| doi_str_mv | 10.1111/j.1600-0897.2006.00365.x |
| format | Article |
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The objective of our study was to correlate the interleukin‐6 (IL‐6) concentrations detected in patient‐collected specimens with provider‐collected specimens and compare the reproducibility of the methods.
Study design
All enrolled participants underwent pelvic examination with collection of cytokine samples by the provider and also collected samples themselves using vaginal swabs. The order of sample collection was randomly assigned. All samples were frozen at −80°C for batch analysis. A commercial enzyme‐linked immunosorbent assay was used to determine the concentrations of IL‐6 in all samples.
Results
IL‐6 concentrations from wicks and swabs were correlated in a linear fashion (r = 0.67, P < 0.001). IL‐6 concentrations in the two swabs (r = 0.94, P < 0.001) and the two wicks (r = 0.71, P < 0.001) were correlated in a linear fashion, although there was more variability in wick specimens.
Conclusion
IL‐6 concentrations can be reproducibly measured using either method. The ease of patient swab collection and the correlation with provider‐collected specimens may make frequent assessment of the vaginal cytokine environment more acceptable to patients.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.1600-0897.2006.00365.x</identifier><identifier>PMID: 16635205</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Cytokines ; Cytokines - isolation & purification ; Female ; Humans ; Inflammation Mediators - analysis ; Inflammation Mediators - isolation & purification ; interleukin-6 ; Interleukin-6 - analysis ; Interleukin-6 - isolation & purification ; Prospective Studies ; Reproducibility of Results ; Specimen Handling - methods ; Specimen Handling - statistics & numerical data ; vagina ; Vagina - immunology ; Vaginal Smears - methods ; Vaginal Smears - statistics & numerical data</subject><ispartof>American journal of reproductive immunology (1989), 2006-05, Vol.55 (5), p.315-320</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4355-b451b869e3e305a1fa2da52d2669b61804711805d5f450cf6ae0fa6327b832e13</citedby><cites>FETCH-LOGICAL-c4355-b451b869e3e305a1fa2da52d2669b61804711805d5f450cf6ae0fa6327b832e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0897.2006.00365.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0897.2006.00365.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16635205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Faro, Constance J.</creatorcontrib><creatorcontrib>Hollier, Lisa M.</creatorcontrib><creatorcontrib>Bishop, Karen</creatorcontrib><title>Comparison of Vaginal Cytokine Collection Methods</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Objective
The objective of our study was to correlate the interleukin‐6 (IL‐6) concentrations detected in patient‐collected specimens with provider‐collected specimens and compare the reproducibility of the methods.
Study design
All enrolled participants underwent pelvic examination with collection of cytokine samples by the provider and also collected samples themselves using vaginal swabs. The order of sample collection was randomly assigned. All samples were frozen at −80°C for batch analysis. A commercial enzyme‐linked immunosorbent assay was used to determine the concentrations of IL‐6 in all samples.
Results
IL‐6 concentrations from wicks and swabs were correlated in a linear fashion (r = 0.67, P < 0.001). IL‐6 concentrations in the two swabs (r = 0.94, P < 0.001) and the two wicks (r = 0.71, P < 0.001) were correlated in a linear fashion, although there was more variability in wick specimens.
Conclusion
IL‐6 concentrations can be reproducibly measured using either method. The ease of patient swab collection and the correlation with provider‐collected specimens may make frequent assessment of the vaginal cytokine environment more acceptable to patients.</description><subject>Cytokines</subject><subject>Cytokines - isolation & purification</subject><subject>Female</subject><subject>Humans</subject><subject>Inflammation Mediators - analysis</subject><subject>Inflammation Mediators - isolation & purification</subject><subject>interleukin-6</subject><subject>Interleukin-6 - analysis</subject><subject>Interleukin-6 - isolation & purification</subject><subject>Prospective Studies</subject><subject>Reproducibility of Results</subject><subject>Specimen Handling - methods</subject><subject>Specimen Handling - statistics & numerical data</subject><subject>vagina</subject><subject>Vagina - immunology</subject><subject>Vaginal Smears - methods</subject><subject>Vaginal Smears - statistics & numerical data</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1PwjAUhhujEUT_gtmVd5un6_pB4g1ZFCGoaPy4bLqt08GguI4I_97OEbzUXrQnPc97mj4IeRgC7NblLMAMwAfR50EIwAIAwmiwOUDdfePQ1RAxn0cgOujE2hmAuyf8GHUwY4SGQLsIx2axUlVhzdIzufeq3oulKr14W5t5sdRebMpSp3Xh2ne6_jCZPUVHuSqtPtudPfRyc_0c3_qTh-EoHkz8NCKU-klEcSJYXxNNgCqcqzBTNMxCxvoJwwIijt1OM5pHFNKcKQ25YiTkiSChxqSHLtq5q8p8rrWt5aKwqS5LtdRmbSXjos8I43-CmGOBKQgHihZMK2NtpXO5qoqFqrYSg2y8ypls9MlGn2y8yh-vcuOi57s31slCZ7_BnUgHXLXAV1Hq7b8Hy8F45AoX99t4YWu92cdVNXf_JJzKt_uhnE6fJpzxRzkm3w4XkyU</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Faro, Constance J.</creator><creator>Hollier, Lisa M.</creator><creator>Bishop, Karen</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200605</creationdate><title>Comparison of Vaginal Cytokine Collection Methods</title><author>Faro, Constance J. ; Hollier, Lisa M. ; Bishop, Karen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4355-b451b869e3e305a1fa2da52d2669b61804711805d5f450cf6ae0fa6327b832e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Cytokines</topic><topic>Cytokines - isolation & purification</topic><topic>Female</topic><topic>Humans</topic><topic>Inflammation Mediators - analysis</topic><topic>Inflammation Mediators - isolation & purification</topic><topic>interleukin-6</topic><topic>Interleukin-6 - analysis</topic><topic>Interleukin-6 - isolation & purification</topic><topic>Prospective Studies</topic><topic>Reproducibility of Results</topic><topic>Specimen Handling - methods</topic><topic>Specimen Handling - statistics & numerical data</topic><topic>vagina</topic><topic>Vagina - immunology</topic><topic>Vaginal Smears - methods</topic><topic>Vaginal Smears - statistics & numerical data</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Faro, Constance J.</creatorcontrib><creatorcontrib>Hollier, Lisa M.</creatorcontrib><creatorcontrib>Bishop, Karen</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Faro, Constance J.</au><au>Hollier, Lisa M.</au><au>Bishop, Karen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Vaginal Cytokine Collection Methods</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2006-05</date><risdate>2006</risdate><volume>55</volume><issue>5</issue><spage>315</spage><epage>320</epage><pages>315-320</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Objective
The objective of our study was to correlate the interleukin‐6 (IL‐6) concentrations detected in patient‐collected specimens with provider‐collected specimens and compare the reproducibility of the methods.
Study design
All enrolled participants underwent pelvic examination with collection of cytokine samples by the provider and also collected samples themselves using vaginal swabs. The order of sample collection was randomly assigned. All samples were frozen at −80°C for batch analysis. A commercial enzyme‐linked immunosorbent assay was used to determine the concentrations of IL‐6 in all samples.
Results
IL‐6 concentrations from wicks and swabs were correlated in a linear fashion (r = 0.67, P < 0.001). IL‐6 concentrations in the two swabs (r = 0.94, P < 0.001) and the two wicks (r = 0.71, P < 0.001) were correlated in a linear fashion, although there was more variability in wick specimens.
Conclusion
IL‐6 concentrations can be reproducibly measured using either method. The ease of patient swab collection and the correlation with provider‐collected specimens may make frequent assessment of the vaginal cytokine environment more acceptable to patients.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16635205</pmid><doi>10.1111/j.1600-0897.2006.00365.x</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 1046-7408 |
| ispartof | American journal of reproductive immunology (1989), 2006-05, Vol.55 (5), p.315-320 |
| issn | 1046-7408 1600-0897 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Cytokines Cytokines - isolation & purification Female Humans Inflammation Mediators - analysis Inflammation Mediators - isolation & purification interleukin-6 Interleukin-6 - analysis Interleukin-6 - isolation & purification Prospective Studies Reproducibility of Results Specimen Handling - methods Specimen Handling - statistics & numerical data vagina Vagina - immunology Vaginal Smears - methods Vaginal Smears - statistics & numerical data |
| title | Comparison of Vaginal Cytokine Collection Methods |
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