Quantitative imaging by confocal scanning fluorescence microscopy of protein crystallization via liquid-liquid phase separation

Metastable states such as liquid–liquid phase separation, aggregation and gelation can affect protein crystallization but their positive or negative effects are only partially understood. In this work, mixtures of PEG (MW 10 kDa) and a large model protein, glucose isomerase (MW 173 kDa), have been s...

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Veröffentlicht in:	Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2005-06, Vol.61 (6), p.819-825
Hauptverfasser:	Vivarès, Denis, Kaler, Eric W., Lenhoff, Abraham M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldose-Ketose Isomerases - chemistry Bacterial Proteins - chemistry crystal growth metastable states Microscopy, Confocal - methods nucleation phase diagram polymorphism Space life sciences Streptomyces - enzymology
Online-Zugang:	Volltext
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