Genetic and Phenotypic Characterization of Haemophilus influenzae Type b Isolated from Children with Meningitis and Their Family Members in Vietnam
To investigate Haemophilus influenzae type b (Hib) infection in Vietnamese children under the age of 5 years, cerebrospinal fluid (CSF) samples from patients with meningitis were screened for Hib, and isolates were subjected to evaluation of susceptibility to 12 antibiotics, biotyping, and genotypin...
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| Veröffentlicht in: | Japanese Journal of Infectious Diseases 2006/04/28, Vol.59(2), pp.111-116 |
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| creator | Huong, Phan L. T. Thi, Ngo T. Anh, Dang D. Huong, Vu T. T. Minh, Le N. Canh, Tran Q. Matsuoka, Mayumi Kamachi, Kazunari Yamazaki, Tsutomu Arakawa, Yoshichika Sasaki, Tsuguo |
| description | To investigate Haemophilus influenzae type b (Hib) infection in Vietnamese children under the age of 5 years, cerebrospinal fluid (CSF) samples from patients with meningitis were screened for Hib, and isolates were subjected to evaluation of susceptibility to 12 antibiotics, biotyping, and genotyping with pulsed-field gel electrophoresis (PFGE). The major biotype was type II (68.3%), followed by type I (22.8%). Among 79 Hib isolates, 45 (57%) were β-lactamase-producing and ampicillin-resistant (44 and 1 isolates produced TEM-1- and ROB-1-type β-lactamases, respectively), and 34 isolates (43%) were β-lactamase-nonproducing and ampicillin-sensitive. No β-lactamase-nonproducing and ampicillin-resistant isolates were found. The PFGE patterns of Hib isolates were highly divergent, but most could be classified into three clusters. We also investigated Hib colonization in household contacts of patients, and found that Hib isolates from the CSF of patients and from nasopharyngeal cavities of household contacts showed the same PFGE patterns. This observation suggested that household contacts of patients are a possible reservoir of Hib. |
| doi_str_mv | 10.7883/yoken.JJID.2006.111 |
| format | Article |
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T. ; Thi, Ngo T. ; Anh, Dang D. ; Huong, Vu T. T. ; Minh, Le N. ; Canh, Tran Q. ; Matsuoka, Mayumi ; Kamachi, Kazunari ; Yamazaki, Tsutomu ; Arakawa, Yoshichika ; Sasaki, Tsuguo</creator><creatorcontrib>Huong, Phan L. T. ; Thi, Ngo T. ; Anh, Dang D. ; Huong, Vu T. T. ; Minh, Le N. ; Canh, Tran Q. ; Matsuoka, Mayumi ; Kamachi, Kazunari ; Yamazaki, Tsutomu ; Arakawa, Yoshichika ; Sasaki, Tsuguo</creatorcontrib><description>To investigate Haemophilus influenzae type b (Hib) infection in Vietnamese children under the age of 5 years, cerebrospinal fluid (CSF) samples from patients with meningitis were screened for Hib, and isolates were subjected to evaluation of susceptibility to 12 antibiotics, biotyping, and genotyping with pulsed-field gel electrophoresis (PFGE). The major biotype was type II (68.3%), followed by type I (22.8%). Among 79 Hib isolates, 45 (57%) were β-lactamase-producing and ampicillin-resistant (44 and 1 isolates produced TEM-1- and ROB-1-type β-lactamases, respectively), and 34 isolates (43%) were β-lactamase-nonproducing and ampicillin-sensitive. No β-lactamase-nonproducing and ampicillin-resistant isolates were found. The PFGE patterns of Hib isolates were highly divergent, but most could be classified into three clusters. We also investigated Hib colonization in household contacts of patients, and found that Hib isolates from the CSF of patients and from nasopharyngeal cavities of household contacts showed the same PFGE patterns. This observation suggested that household contacts of patients are a possible reservoir of Hib.</description><identifier>ISSN: 1344-6304</identifier><identifier>EISSN: 1884-2836</identifier><identifier>DOI: 10.7883/yoken.JJID.2006.111</identifier><identifier>PMID: 16632911</identifier><language>eng</language><publisher>Japan: National Institute of Infectious Diseases</publisher><subject>Adolescent ; Adult ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - therapeutic use ; Cerebrospinal Fluid - microbiology ; Child ; Child, Preschool ; Cluster Analysis ; Disease Reservoirs - microbiology ; Dose-Response Relationship, Drug ; Drug Resistance, Bacterial ; Electrophoresis, Gel, Pulsed-Field ; Female ; Haemophilus influenzae type b - classification ; Haemophilus influenzae type b - drug effects ; Haemophilus influenzae type b - genetics ; Haemophilus influenzae type b - isolation & purification ; Humans ; Infant ; Male ; Meningitis, Haemophilus - diagnosis ; Meningitis, Haemophilus - drug therapy ; Meningitis, Haemophilus - microbiology ; Microbial Sensitivity Tests ; Phenotype ; Phylogeny ; Treatment Outcome ; Vietnam</subject><ispartof>Japanese Journal of Infectious Diseases, 2006/04/28, Vol.59(2), pp.111-116</ispartof><rights>2006 Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-36ae17e1f04005c5dc82f129ded1fcde2bb49af6b037719749a23b0c4dda59c83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16632911$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huong, Phan L. T.</creatorcontrib><creatorcontrib>Thi, Ngo T.</creatorcontrib><creatorcontrib>Anh, Dang D.</creatorcontrib><creatorcontrib>Huong, Vu T. T.</creatorcontrib><creatorcontrib>Minh, Le N.</creatorcontrib><creatorcontrib>Canh, Tran Q.</creatorcontrib><creatorcontrib>Matsuoka, Mayumi</creatorcontrib><creatorcontrib>Kamachi, Kazunari</creatorcontrib><creatorcontrib>Yamazaki, Tsutomu</creatorcontrib><creatorcontrib>Arakawa, Yoshichika</creatorcontrib><creatorcontrib>Sasaki, Tsuguo</creatorcontrib><title>Genetic and Phenotypic Characterization of Haemophilus influenzae Type b Isolated from Children with Meningitis and Their Family Members in Vietnam</title><title>Japanese Journal of Infectious Diseases</title><addtitle>Jpn J Infect Dis</addtitle><description>To investigate Haemophilus influenzae type b (Hib) infection in Vietnamese children under the age of 5 years, cerebrospinal fluid (CSF) samples from patients with meningitis were screened for Hib, and isolates were subjected to evaluation of susceptibility to 12 antibiotics, biotyping, and genotyping with pulsed-field gel electrophoresis (PFGE). The major biotype was type II (68.3%), followed by type I (22.8%). Among 79 Hib isolates, 45 (57%) were β-lactamase-producing and ampicillin-resistant (44 and 1 isolates produced TEM-1- and ROB-1-type β-lactamases, respectively), and 34 isolates (43%) were β-lactamase-nonproducing and ampicillin-sensitive. No β-lactamase-nonproducing and ampicillin-resistant isolates were found. The PFGE patterns of Hib isolates were highly divergent, but most could be classified into three clusters. We also investigated Hib colonization in household contacts of patients, and found that Hib isolates from the CSF of patients and from nasopharyngeal cavities of household contacts showed the same PFGE patterns. This observation suggested that household contacts of patients are a possible reservoir of Hib.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Cerebrospinal Fluid - microbiology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cluster Analysis</subject><subject>Disease Reservoirs - microbiology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Resistance, Bacterial</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Female</subject><subject>Haemophilus influenzae type b - classification</subject><subject>Haemophilus influenzae type b - drug effects</subject><subject>Haemophilus influenzae type b - genetics</subject><subject>Haemophilus influenzae type b - isolation & purification</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Meningitis, Haemophilus - diagnosis</subject><subject>Meningitis, Haemophilus - drug therapy</subject><subject>Meningitis, Haemophilus - microbiology</subject><subject>Microbial Sensitivity Tests</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Treatment Outcome</subject><subject>Vietnam</subject><issn>1344-6304</issn><issn>1884-2836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1u1DAYRSNERUvpEyAhr9hl8E_-vEQDbadqBYuhW8uxvzQuiR1sRyh9DV4YpzMqYmP7k8-9lnyy7D3Bm7pp2KfF_QS7ubnZfdlQjKsNIeRVdkaapshpw6rX6cyKIq8YLk6ztyE8YkzLkuA32SmpKkY5IWfZnyuwEI1C0mr0vQfr4jKlcdtLL1UEb55kNM4i16FrCaObejPMARnbDTPYJwlov0yAWrQLbpARNOq8G1PeDNqDRb9N7NEdWGMfTDTh-Z19D8ajSzmaYUl3Ywt-bUT3BqKV47vspJNDgIvjfp79uPy6317nt9-udtvPt7kqcRFzVkkgNZAOFxiXqtSqoR2hXIMmndJA27bgsqtazOqa8DoNlLVYFVrLkquGnWcfD72Td79mCFGMJigYBmnBzUFUdcMJ5yyB7AAq70Lw0InJm1H6RRAsVhfi2YVYXYjVhUguUurDsX5uR9D_MsfPT8DuADyGKB_gBZA-CRngWFpyQdflv_IXRiVPAiz7C-twpFk</recordid><startdate>20060428</startdate><enddate>20060428</enddate><creator>Huong, Phan L. 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T.</creatorcontrib><creatorcontrib>Thi, Ngo T.</creatorcontrib><creatorcontrib>Anh, Dang D.</creatorcontrib><creatorcontrib>Huong, Vu T. T.</creatorcontrib><creatorcontrib>Minh, Le N.</creatorcontrib><creatorcontrib>Canh, Tran Q.</creatorcontrib><creatorcontrib>Matsuoka, Mayumi</creatorcontrib><creatorcontrib>Kamachi, Kazunari</creatorcontrib><creatorcontrib>Yamazaki, Tsutomu</creatorcontrib><creatorcontrib>Arakawa, Yoshichika</creatorcontrib><creatorcontrib>Sasaki, Tsuguo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese Journal of Infectious Diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huong, Phan L. T.</au><au>Thi, Ngo T.</au><au>Anh, Dang D.</au><au>Huong, Vu T. T.</au><au>Minh, Le N.</au><au>Canh, Tran Q.</au><au>Matsuoka, Mayumi</au><au>Kamachi, Kazunari</au><au>Yamazaki, Tsutomu</au><au>Arakawa, Yoshichika</au><au>Sasaki, Tsuguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic and Phenotypic Characterization of Haemophilus influenzae Type b Isolated from Children with Meningitis and Their Family Members in Vietnam</atitle><jtitle>Japanese Journal of Infectious Diseases</jtitle><addtitle>Jpn J Infect Dis</addtitle><date>2006-04-28</date><risdate>2006</risdate><volume>59</volume><issue>2</issue><spage>111</spage><epage>116</epage><pages>111-116</pages><artnum>JJID.2006.111</artnum><issn>1344-6304</issn><eissn>1884-2836</eissn><abstract>To investigate Haemophilus influenzae type b (Hib) infection in Vietnamese children under the age of 5 years, cerebrospinal fluid (CSF) samples from patients with meningitis were screened for Hib, and isolates were subjected to evaluation of susceptibility to 12 antibiotics, biotyping, and genotyping with pulsed-field gel electrophoresis (PFGE). The major biotype was type II (68.3%), followed by type I (22.8%). Among 79 Hib isolates, 45 (57%) were β-lactamase-producing and ampicillin-resistant (44 and 1 isolates produced TEM-1- and ROB-1-type β-lactamases, respectively), and 34 isolates (43%) were β-lactamase-nonproducing and ampicillin-sensitive. No β-lactamase-nonproducing and ampicillin-resistant isolates were found. The PFGE patterns of Hib isolates were highly divergent, but most could be classified into three clusters. We also investigated Hib colonization in household contacts of patients, and found that Hib isolates from the CSF of patients and from nasopharyngeal cavities of household contacts showed the same PFGE patterns. This observation suggested that household contacts of patients are a possible reservoir of Hib.</abstract><cop>Japan</cop><pub>National Institute of Infectious Diseases</pub><pmid>16632911</pmid><doi>10.7883/yoken.JJID.2006.111</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Japanese Journal of Infectious Diseases, 2006/04/28, Vol.59(2), pp.111-116 |
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| source | Freely Accessible Japanese Titles (ERDB Project); J-STAGE (Free - Japanese); MEDLINE; EZB Electronic Journals Library |
| subjects | Adolescent Adult Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Cerebrospinal Fluid - microbiology Child Child, Preschool Cluster Analysis Disease Reservoirs - microbiology Dose-Response Relationship, Drug Drug Resistance, Bacterial Electrophoresis, Gel, Pulsed-Field Female Haemophilus influenzae type b - classification Haemophilus influenzae type b - drug effects Haemophilus influenzae type b - genetics Haemophilus influenzae type b - isolation & purification Humans Infant Male Meningitis, Haemophilus - diagnosis Meningitis, Haemophilus - drug therapy Meningitis, Haemophilus - microbiology Microbial Sensitivity Tests Phenotype Phylogeny Treatment Outcome Vietnam |
| title | Genetic and Phenotypic Characterization of Haemophilus influenzae Type b Isolated from Children with Meningitis and Their Family Members in Vietnam |
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