Structural organization of astrocytes in the rat hippocampus in the post-ischemic period
The aim of the present work was to study the location and structural organization of astrocytes in the rat hippocampus, which contain immunoreactive glial fibrillary acid protein (GFAP) after ischemic damage to the brain after intracerebroventricular administration of the neuroprotective agent creat...
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| Veröffentlicht in: | Neuroscience and behavioral physiology 2005-05, Vol.35 (4), p.389-392 |
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| creator | Korzhevskii, D E Otellin, V A Grigor'ev, I P Kostkin, V B Polenov, S A Lentsman, M V Balestrino, M |
| description | The aim of the present work was to study the location and structural organization of astrocytes in the rat hippocampus, which contain immunoreactive glial fibrillary acid protein (GFAP) after ischemic damage to the brain after intracerebroventricular administration of the neuroprotective agent creatine and without treatment. Light microscopy and immunocytochemical methods were used to study the brains of 26 adult male Sprague-Dawley (Koltushi) rats, some of which were subjected to total cerebral ischemia (12 min) under anesthesia with subsequent reperfusion (seven days). Creatine was given to 11 animals intracerebroventricularly using an osmotic pump (Alzet Osmotic Mini-Pump). The results showed that GFAP-immunoreactive hippocampal astrocytes were concentrated in two main zones (the stratum lacunosum-moleculare of field CA1 and the stratum polymorphae of the dentate fascia). The neuroprotective effect of creatine had the result that moderate ischemic damage to the hippocampus did not lead to changes in the zones containing activated astrocytes. The redistribution of GFAP-positive astrocytes in the post-ischemic period was associated with loss of pyramidal neurons in cytoarchitectonic field CA1. Complete loss of pyramidal neurons in this area of the hippocampus leads to a qualitatively new level of astrocyte activation--proliferation. |
| doi_str_mv | 10.1007/s11055-005-0038-9 |
| format | Article |
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Light microscopy and immunocytochemical methods were used to study the brains of 26 adult male Sprague-Dawley (Koltushi) rats, some of which were subjected to total cerebral ischemia (12 min) under anesthesia with subsequent reperfusion (seven days). Creatine was given to 11 animals intracerebroventricularly using an osmotic pump (Alzet Osmotic Mini-Pump). The results showed that GFAP-immunoreactive hippocampal astrocytes were concentrated in two main zones (the stratum lacunosum-moleculare of field CA1 and the stratum polymorphae of the dentate fascia). The neuroprotective effect of creatine had the result that moderate ischemic damage to the hippocampus did not lead to changes in the zones containing activated astrocytes. The redistribution of GFAP-positive astrocytes in the post-ischemic period was associated with loss of pyramidal neurons in cytoarchitectonic field CA1. Complete loss of pyramidal neurons in this area of the hippocampus leads to a qualitatively new level of astrocyte activation--proliferation.</description><identifier>ISSN: 0097-0549</identifier><identifier>EISSN: 1573-899X</identifier><identifier>DOI: 10.1007/s11055-005-0038-9</identifier><identifier>PMID: 15929566</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Animals ; Astrocytes - drug effects ; Astrocytes - metabolism ; Astrocytes - pathology ; Cell Count - methods ; Creatine - administration & dosage ; Glial Fibrillary Acidic Protein - metabolism ; Hippocampus - drug effects ; Hippocampus - metabolism ; Hippocampus - pathology ; Immunohistochemistry - methods ; Injections, Intraventricular - methods ; Ischemia - drug therapy ; Ischemia - metabolism ; Ischemia - pathology ; Male ; Neuroprotective Agents - administration & dosage ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Neuroscience and behavioral physiology, 2005-05, Vol.35 (4), p.389-392</ispartof><rights>Springer Science+Business Media, Inc. 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-e2c01c53a3176611de66b9e50c3ad096643f1a85e8f13a1f4027043666a638443</citedby><cites>FETCH-LOGICAL-c272t-e2c01c53a3176611de66b9e50c3ad096643f1a85e8f13a1f4027043666a638443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15929566$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Korzhevskii, D E</creatorcontrib><creatorcontrib>Otellin, V A</creatorcontrib><creatorcontrib>Grigor'ev, I P</creatorcontrib><creatorcontrib>Kostkin, V B</creatorcontrib><creatorcontrib>Polenov, S A</creatorcontrib><creatorcontrib>Lentsman, M V</creatorcontrib><creatorcontrib>Balestrino, M</creatorcontrib><title>Structural organization of astrocytes in the rat hippocampus in the post-ischemic period</title><title>Neuroscience and behavioral physiology</title><addtitle>Neurosci Behav Physiol</addtitle><description>The aim of the present work was to study the location and structural organization of astrocytes in the rat hippocampus, which contain immunoreactive glial fibrillary acid protein (GFAP) after ischemic damage to the brain after intracerebroventricular administration of the neuroprotective agent creatine and without treatment. 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Complete loss of pyramidal neurons in this area of the hippocampus leads to a qualitatively new level of astrocyte activation--proliferation.</description><subject>Animals</subject><subject>Astrocytes - drug effects</subject><subject>Astrocytes - metabolism</subject><subject>Astrocytes - pathology</subject><subject>Cell Count - methods</subject><subject>Creatine - administration & dosage</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - pathology</subject><subject>Immunohistochemistry - methods</subject><subject>Injections, Intraventricular - methods</subject><subject>Ischemia - drug therapy</subject><subject>Ischemia - metabolism</subject><subject>Ischemia - pathology</subject><subject>Male</subject><subject>Neuroprotective Agents - administration & dosage</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0097-0549</issn><issn>1573-899X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1KAzEURoMotlYfwI0MLtyN3ptMkslSxD8ouFChu5CmGTulMxmTzKI-vVNaFNy4CIHkfB_3cgg5R7hGAHkTEYHzHGB7WJmrAzJGLlleKjU7JGMAJXPghRqRkxhXMGRkCcdkhFxRxYUYk9lrCr1NfTDrzIcP09ZfJtW-zXyVmZiCt5vkYla3WVq6LJiULeuu89Y0Xf_z3PmY8jrapWtqm3Uu1H5xSo4qs47ubH9PyPvD_dvdUz59eXy-u53mlkqackctoOXMMJRCIC6cEHPlOFhmFqCEKFiFpuSurJAZrAqgEgomhDCClUXBJuRq19sF_9m7mHQzTOLWa9M630ctZKkAkf8LomQKKaUDePkHXPk-tMMSevtd0KLEAcIdZIOPMbhKd6FuTNhoBL2Vo3dy9CBHb-VoNWQu9sX9vHGL38TeBvsGvZyJUA</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>Korzhevskii, D E</creator><creator>Otellin, V A</creator><creator>Grigor'ev, I P</creator><creator>Kostkin, V B</creator><creator>Polenov, S A</creator><creator>Lentsman, M V</creator><creator>Balestrino, M</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>200505</creationdate><title>Structural organization of astrocytes in the rat hippocampus in the post-ischemic period</title><author>Korzhevskii, D E ; Otellin, V A ; Grigor'ev, I P ; Kostkin, V B ; Polenov, S A ; Lentsman, M V ; Balestrino, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-e2c01c53a3176611de66b9e50c3ad096643f1a85e8f13a1f4027043666a638443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Astrocytes - 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| subjects | Animals Astrocytes - drug effects Astrocytes - metabolism Astrocytes - pathology Cell Count - methods Creatine - administration & dosage Glial Fibrillary Acidic Protein - metabolism Hippocampus - drug effects Hippocampus - metabolism Hippocampus - pathology Immunohistochemistry - methods Injections, Intraventricular - methods Ischemia - drug therapy Ischemia - metabolism Ischemia - pathology Male Neuroprotective Agents - administration & dosage Rats Rats, Sprague-Dawley |
| title | Structural organization of astrocytes in the rat hippocampus in the post-ischemic period |
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