DNA Shuttling Between Plasmid Vectors and a Genome Vector: Systematic Conversion and Preservation of DNA Libraries Using the Bacillus subtilis Genome (BGM) Vector
The combined use of the contemporary vector systems, the bacterial artificial chromosome (BAC) vector and the Bacillus subtilis genome (BGM) vector, makes possible the handling of giant-length DNA (above 100 kb). Our newly constructed BGM vector efficiently integrated DNA prepared in the BAC vector....
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| Veröffentlicht in: | Journal of molecular biology 2005-06, Vol.349 (5), p.1036-1044 |
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| container_issue | 5 |
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| container_title | Journal of molecular biology |
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| creator | Kaneko, Shinya Akioka, Manami Tsuge, Kenji Itaya, Mitsuhiro |
| description | The combined use of the contemporary vector systems, the bacterial artificial chromosome (BAC) vector and the
Bacillus subtilis genome (BGM) vector, makes possible the handling of giant-length DNA (above 100
kb). Our newly constructed BGM vector efficiently integrated DNA prepared in the BAC vector. A BAC library comprised of 18 independent clones prepared from mitochondrial DNA (mtDNA) of
Arabidopsis thaliana was converted to a parallel BGM library using the new BGM vector. The effectiveness of the combined use of the vector systems was confirmed by the stable recovery of all 18 DNAs as BAC clones from the respective BGM clones. We show that DNA in BGM was stably preserved at room temperature after spore formation of the host
B.
subtilis
. Rapid and stable shuttling between
Escherichia
coli
and the
B.
subtilis
host, combined with spore-mediated DNA storage, may facilitate the long-term and low-cost preservation and the transportation of DNA resources. |
| doi_str_mv | 10.1016/j.jmb.2005.04.041 |
| format | Article |
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Bacillus subtilis genome (BGM) vector, makes possible the handling of giant-length DNA (above 100
kb). Our newly constructed BGM vector efficiently integrated DNA prepared in the BAC vector. A BAC library comprised of 18 independent clones prepared from mitochondrial DNA (mtDNA) of
Arabidopsis thaliana was converted to a parallel BGM library using the new BGM vector. The effectiveness of the combined use of the vector systems was confirmed by the stable recovery of all 18 DNAs as BAC clones from the respective BGM clones. We show that DNA in BGM was stably preserved at room temperature after spore formation of the host
B.
subtilis
. Rapid and stable shuttling between
Escherichia
coli
and the
B.
subtilis
host, combined with spore-mediated DNA storage, may facilitate the long-term and low-cost preservation and the transportation of DNA resources.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/j.jmb.2005.04.041</identifier><identifier>PMID: 15913652</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Arabidopsis - genetics ; Arabidopsis thaliana ; Bacillus subtilis ; Bacillus subtilis - genetics ; Chromosomes, Artificial, Bacterial ; DNA, Mitochondrial - genetics ; Escherichia coli - genetics ; Gene Library ; Genetic Vectors ; genome vector ; Genome, Bacterial ; Plasmids ; recombination ; recombinational transfer ; spore formation ; Spores, Bacterial ; transformation</subject><ispartof>Journal of molecular biology, 2005-06, Vol.349 (5), p.1036-1044</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-7a8b5f48bb307461ce88335fadfdad5d08ae00b99eeef630809323d2a48026f63</citedby><cites>FETCH-LOGICAL-c479t-7a8b5f48bb307461ce88335fadfdad5d08ae00b99eeef630809323d2a48026f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jmb.2005.04.041$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15913652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaneko, Shinya</creatorcontrib><creatorcontrib>Akioka, Manami</creatorcontrib><creatorcontrib>Tsuge, Kenji</creatorcontrib><creatorcontrib>Itaya, Mitsuhiro</creatorcontrib><title>DNA Shuttling Between Plasmid Vectors and a Genome Vector: Systematic Conversion and Preservation of DNA Libraries Using the Bacillus subtilis Genome (BGM) Vector</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>The combined use of the contemporary vector systems, the bacterial artificial chromosome (BAC) vector and the
Bacillus subtilis genome (BGM) vector, makes possible the handling of giant-length DNA (above 100
kb). Our newly constructed BGM vector efficiently integrated DNA prepared in the BAC vector. A BAC library comprised of 18 independent clones prepared from mitochondrial DNA (mtDNA) of
Arabidopsis thaliana was converted to a parallel BGM library using the new BGM vector. The effectiveness of the combined use of the vector systems was confirmed by the stable recovery of all 18 DNAs as BAC clones from the respective BGM clones. We show that DNA in BGM was stably preserved at room temperature after spore formation of the host
B.
subtilis
. Rapid and stable shuttling between
Escherichia
coli
and the
B.
subtilis
host, combined with spore-mediated DNA storage, may facilitate the long-term and low-cost preservation and the transportation of DNA resources.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - genetics</subject><subject>Chromosomes, Artificial, Bacterial</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Gene Library</subject><subject>Genetic Vectors</subject><subject>genome vector</subject><subject>Genome, Bacterial</subject><subject>Plasmids</subject><subject>recombination</subject><subject>recombinational transfer</subject><subject>spore formation</subject><subject>Spores, Bacterial</subject><subject>transformation</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EokvhB3BBPiE4ZBnHiePAqbuFBWmBSqVcLSeeUK-SuNjOov6d_lK8bBA3Ko1k6fmb96R5hDxnsGTAxJvdcjc0yxygXEKRhj0gCwayzqTg8iFZAOR5lksuTsiTEHaQQF7Ix-SElTXjoswX5O78yxm9vJ5i7O34g64w_kIc6UWvw2AN_Y5tdD5QPRqq6QZHN-AsvqWXtyHioKNt6dqNe_TBuvEPeuExoN-nryS4jh5Ctrbx2lsM9CocouI10pVubd9PgYapiba34W_Eq9Xm8-s56Cl51Ok-4LP5PSVXH95_W3_Mtl83n9Zn26wtqjpmlZZN2RWyaThUhWAtSsl52WnTGW1KA1IjQFPXiNgJDhJqnnOT60JCLpJySl4efW-8-zlhiGqwocW-1yO6KShRSVmL8n6Q1RJEkczvBStelEJAAtkRbL0LwWOnbrwdtL9VDNSharVTqWp1qFpBkYalnRez-dQMaP5tzN0m4N0RwHS0vUWvQmtxbNFYnw6rjLP_sf8NHyq6Nw</recordid><startdate>20050624</startdate><enddate>20050624</enddate><creator>Kaneko, Shinya</creator><creator>Akioka, Manami</creator><creator>Tsuge, Kenji</creator><creator>Itaya, Mitsuhiro</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050624</creationdate><title>DNA Shuttling Between Plasmid Vectors and a Genome Vector: Systematic Conversion and Preservation of DNA Libraries Using the Bacillus subtilis Genome (BGM) Vector</title><author>Kaneko, Shinya ; Akioka, Manami ; Tsuge, Kenji ; Itaya, Mitsuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-7a8b5f48bb307461ce88335fadfdad5d08ae00b99eeef630809323d2a48026f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis thaliana</topic><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - genetics</topic><topic>Chromosomes, Artificial, Bacterial</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Gene Library</topic><topic>Genetic Vectors</topic><topic>genome vector</topic><topic>Genome, Bacterial</topic><topic>Plasmids</topic><topic>recombination</topic><topic>recombinational transfer</topic><topic>spore formation</topic><topic>Spores, Bacterial</topic><topic>transformation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaneko, Shinya</creatorcontrib><creatorcontrib>Akioka, Manami</creatorcontrib><creatorcontrib>Tsuge, Kenji</creatorcontrib><creatorcontrib>Itaya, Mitsuhiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaneko, Shinya</au><au>Akioka, Manami</au><au>Tsuge, Kenji</au><au>Itaya, Mitsuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Shuttling Between Plasmid Vectors and a Genome Vector: Systematic Conversion and Preservation of DNA Libraries Using the Bacillus subtilis Genome (BGM) Vector</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>2005-06-24</date><risdate>2005</risdate><volume>349</volume><issue>5</issue><spage>1036</spage><epage>1044</epage><pages>1036-1044</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>The combined use of the contemporary vector systems, the bacterial artificial chromosome (BAC) vector and the
Bacillus subtilis genome (BGM) vector, makes possible the handling of giant-length DNA (above 100
kb). Our newly constructed BGM vector efficiently integrated DNA prepared in the BAC vector. A BAC library comprised of 18 independent clones prepared from mitochondrial DNA (mtDNA) of
Arabidopsis thaliana was converted to a parallel BGM library using the new BGM vector. The effectiveness of the combined use of the vector systems was confirmed by the stable recovery of all 18 DNAs as BAC clones from the respective BGM clones. We show that DNA in BGM was stably preserved at room temperature after spore formation of the host
B.
subtilis
. Rapid and stable shuttling between
Escherichia
coli
and the
B.
subtilis
host, combined with spore-mediated DNA storage, may facilitate the long-term and low-cost preservation and the transportation of DNA resources.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15913652</pmid><doi>10.1016/j.jmb.2005.04.041</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of molecular biology, 2005-06, Vol.349 (5), p.1036-1044 |
| issn | 0022-2836 1089-8638 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Arabidopsis - genetics Arabidopsis thaliana Bacillus subtilis Bacillus subtilis - genetics Chromosomes, Artificial, Bacterial DNA, Mitochondrial - genetics Escherichia coli - genetics Gene Library Genetic Vectors genome vector Genome, Bacterial Plasmids recombination recombinational transfer spore formation Spores, Bacterial transformation |
| title | DNA Shuttling Between Plasmid Vectors and a Genome Vector: Systematic Conversion and Preservation of DNA Libraries Using the Bacillus subtilis Genome (BGM) Vector |
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