Detection of Fragmented Genomic DNA by PCR-Free Piezoelectric Sensing Using a Denaturation Approach
Label-free and real-time DNA sequence detection in PCR-amplified DNA samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of seq...
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| Veröffentlicht in: | Journal of the American Chemical Society 2005-06, Vol.127 (22), p.7966-7967 |
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| container_end_page | 7967 |
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| container_issue | 22 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 127 |
| creator | Minunni, Maria Tombelli, Sara Fonti, Jessica Spiriti, Maria M. Mascini, Marco Bogani, Patrizia Buiatti, Marcello |
| description | Label-free and real-time DNA sequence detection in PCR-amplified DNA samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of sequences in nonamplified genomic DNA is described. The system relies on real-time and label-free detection of the hybridization reaction between an immobilized probe and the complementary sequence in solution. The DNA probe is immobilized on the sensing surface (10 MHz quartz crystals), while the complementary sequence is present in the genomic DNA, previously fragmented with restriction enzymes. |
| doi_str_mv | 10.1021/ja051345q |
| format | Article |
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On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of sequences in nonamplified genomic DNA is described. The system relies on real-time and label-free detection of the hybridization reaction between an immobilized probe and the complementary sequence in solution. The DNA probe is immobilized on the sensing surface (10 MHz quartz crystals), while the complementary sequence is present in the genomic DNA, previously fragmented with restriction enzymes.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja051345q</identifier><identifier>PMID: 15926792</identifier><identifier>CODEN: JACSAT</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological and medical sciences ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; DNA, Plant - analysis ; DNA, Plant - chemistry ; DNA, Plant - genetics ; DNA, Single-Stranded - analysis ; DNA, Single-Stranded - chemistry ; Electrochemistry - methods ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. 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Am. Chem. Soc</addtitle><description>Label-free and real-time DNA sequence detection in PCR-amplified DNA samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of sequences in nonamplified genomic DNA is described. The system relies on real-time and label-free detection of the hybridization reaction between an immobilized probe and the complementary sequence in solution. The DNA probe is immobilized on the sensing surface (10 MHz quartz crystals), while the complementary sequence is present in the genomic DNA, previously fragmented with restriction enzymes.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>DNA, Plant - analysis</subject><subject>DNA, Plant - chemistry</subject><subject>DNA, Plant - genetics</subject><subject>DNA, Single-Stranded - analysis</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>Electrochemistry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Nicotiana - genetics</subject><subject>Nucleic Acid Denaturation</subject><subject>Oligonucleotides - chemistry</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Various methods and equipments</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMFu1DAQhi0EokvhwAsgX0DiEGo7cewctxu2IJV21W0lbtbEmZQsibO1E6nt0-N2V90LlxmN5pt_Zn5CPnL2jTPBTzbAJE8zefeKzLgULJFc5K_JjDEmEqXz9Ii8C2ETy0xo_pYccVmIXBViRmyJI9qxHRwdGrr0cNujG7GmZ-iGvrW0vJjT6oGuFlfJ0iPSVYuPA3ZxxsfuGl1o3S29eY5AS3QwTh6eBefbrR_A_nlP3jTQBfywz8fkZvn9evEjOb88-7mYnyeQcTEmjZUg8tqitrbQKmZhRSW0UiChUhJqsLUSQqU6_sFEDQ1XRcagYBmzlUiPyZedblx7N2EYTd8Gi10HDocpmFxprTnjEfy6A60fQvDYmK1ve_APhjPz5Kh5cTSyn_aiU9VjfSD3Fkbg8x6AYKFrPDjbhgOXa5nrgkUu2XFtGPH-pQ_-bzwsVdJcr9Zmzcvfp1l5ZX4ddMEGsxkm76J3_znwHxWxmGQ</recordid><startdate>20050608</startdate><enddate>20050608</enddate><creator>Minunni, Maria</creator><creator>Tombelli, Sara</creator><creator>Fonti, Jessica</creator><creator>Spiriti, Maria M.</creator><creator>Mascini, Marco</creator><creator>Bogani, Patrizia</creator><creator>Buiatti, Marcello</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050608</creationdate><title>Detection of Fragmented Genomic DNA by PCR-Free Piezoelectric Sensing Using a Denaturation Approach</title><author>Minunni, Maria ; Tombelli, Sara ; Fonti, Jessica ; Spiriti, Maria M. ; Mascini, Marco ; Bogani, Patrizia ; Buiatti, Marcello</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a412t-fc5a26dce8cc987ce82c2b2877a5ab75adacd72273804202daf17940a9040cb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>DNA, Plant - analysis</topic><topic>DNA, Plant - chemistry</topic><topic>DNA, Plant - genetics</topic><topic>DNA, Single-Stranded - analysis</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>Electrochemistry - methods</topic><topic>Fundamental and applied biological sciences. 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Am. Chem. Soc</addtitle><date>2005-06-08</date><risdate>2005</risdate><volume>127</volume><issue>22</issue><spage>7966</spage><epage>7967</epage><pages>7966-7967</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><coden>JACSAT</coden><abstract>Label-free and real-time DNA sequence detection in PCR-amplified DNA samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of sequences in nonamplified genomic DNA is described. The system relies on real-time and label-free detection of the hybridization reaction between an immobilized probe and the complementary sequence in solution. 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| subjects | Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology DNA, Plant - analysis DNA, Plant - chemistry DNA, Plant - genetics DNA, Single-Stranded - analysis DNA, Single-Stranded - chemistry Electrochemistry - methods Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Nicotiana - genetics Nucleic Acid Denaturation Oligonucleotides - chemistry Polymerase Chain Reaction - methods Various methods and equipments |
| title | Detection of Fragmented Genomic DNA by PCR-Free Piezoelectric Sensing Using a Denaturation Approach |
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