Nuclear Pores Form de Novo from Both Sides of the Nuclear Envelope
Nuclear pore complexes are multiprotein channels that span the double lipid bilayer of the nuclear envelope. How new pores are inserted into the intact nuclear envelope of proliferating and differentiating eukaryotic cells is unknown. We found that the Nup107-160 complex was incorporated into assemb...
Gespeichert in:
| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2006-04, Vol.312 (5772), p.440-443 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 443 |
|---|---|
| container_issue | 5772 |
| container_start_page | 440 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 312 |
| creator | D'Angelo, Maximiliano A Anderson, Daniel J Richard, Erin Hetzer, Martin W |
| description | Nuclear pore complexes are multiprotein channels that span the double lipid bilayer of the nuclear envelope. How new pores are inserted into the intact nuclear envelope of proliferating and differentiating eukaryotic cells is unknown. We found that the Nup107-160 complex was incorporated into assembly sites in the nuclear envelope from both the nucleoplasmic and the cytoplasmic sides. Nuclear pore insertion required the generation of Ran guanosine triphosphate in the nuclear and cytoplasmic compartments. Newly formed nuclear pore complexes did not contain structural components of preexisting pores, suggesting that they can form de novo. |
| doi_str_mv | 10.1126/science.1124196 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_67884570</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>3845890</jstor_id><sourcerecordid>3845890</sourcerecordid><originalsourceid>FETCH-LOGICAL-c555t-42fbf313f9db2b82f5fe92f3a94b3c9c490592004e3081b3c4ac95d74e8fcaa33</originalsourceid><addsrcrecordid>eNqFkUtr3DAUhUVpSCaPdTelNYVk5-TqZUnLzJBpAyEppFkLWZYaD7Y1kexA_301jGmgm6yEdL57uDoHoU8YLjEm1VWyrRus210YVtUHtMCgeKkI0I9oAUCrUoLgR-g4pQ1A1hQ9REe4qogQjC_Q8n6ynTOx-BmiS8U6xL5oXHEfXkPhY-iLZRifi8e2yWLwxfictXniZnh1Xdi6U3TgTZfc2XyeoKf1za_Vj_Lu4fvt6vqutJzzsWTE155i6lVTk1oSz71TxFOjWE2tskwBz3sDcxQkzk_MWMUbwZz01hhKT9DF3ncbw8vk0qj7NlnXdWZwYUq6ElIyLuBdkEjgUonqXRALTCmGHfjtP3ATpjjk32qCKZecEZKhqz1kY0gpOq-3se1N_KMx6F1bem5Lz23liS-z7VT3rnnj53oycD4DJlnT-WgG26Y3TghOCGGZ-7znNmkM8Z9Ocx5S7QL5upe9Cdr8jtni6ZEApoBBQM6d_gWrC644</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213585422</pqid></control><display><type>article</type><title>Nuclear Pores Form de Novo from Both Sides of the Nuclear Envelope</title><source>American Association for the Advancement of Science</source><source>Jstor Complete Legacy</source><source>MEDLINE</source><creator>D'Angelo, Maximiliano A ; Anderson, Daniel J ; Richard, Erin ; Hetzer, Martin W</creator><creatorcontrib>D'Angelo, Maximiliano A ; Anderson, Daniel J ; Richard, Erin ; Hetzer, Martin W</creatorcontrib><description>Nuclear pore complexes are multiprotein channels that span the double lipid bilayer of the nuclear envelope. How new pores are inserted into the intact nuclear envelope of proliferating and differentiating eukaryotic cells is unknown. We found that the Nup107-160 complex was incorporated into assembly sites in the nuclear envelope from both the nucleoplasmic and the cytoplasmic sides. Nuclear pore insertion required the generation of Ran guanosine triphosphate in the nuclear and cytoplasmic compartments. Newly formed nuclear pore complexes did not contain structural components of preexisting pores, suggesting that they can form de novo.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1124196</identifier><identifier>PMID: 16627745</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Active Transport, Cell Nucleus ; Animals ; beta Karyopherins - metabolism ; beta Karyopherins - pharmacology ; Biological and medical sciences ; Cell lines ; Cell membranes. Ionic channels. Membrane pores ; Cell structures and functions ; Cell-Free System ; Cytoplasm - metabolism ; Cytosol ; Egtazic Acid - analogs & derivatives ; Egtazic Acid - pharmacology ; Eukaryotes ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Guanosine Triphosphate - metabolism ; HeLa Cells ; Humans ; Image Processing, Computer-Assisted ; Karyopherins ; Lipids ; Microscopy ; Microscopy, Confocal ; Molecular and cellular biology ; Nuclear Envelope - metabolism ; Nuclear Envelope - ultrastructure ; Nuclear membrane ; Nuclear pore ; Nuclear Pore - metabolism ; Nuclear Pore - ultrastructure ; Nuclear pore complex proteins ; Nuclear Pore Complex Proteins - metabolism ; Proteins ; ran GTP-Binding Protein - metabolism ; Recombinant Proteins - metabolism ; Signal detection ; Spermatozoa ; Wheat Germ Agglutinins - metabolism ; Wheat Germ Agglutinins - pharmacology ; Xenopus</subject><ispartof>Science (American Association for the Advancement of Science), 2006-04, Vol.312 (5772), p.440-443</ispartof><rights>Copyright 2006 American Association for the Advancement of Science</rights><rights>2006 INIST-CNRS</rights><rights>Copyright American Association for the Advancement of Science Apr 21, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c555t-42fbf313f9db2b82f5fe92f3a94b3c9c490592004e3081b3c4ac95d74e8fcaa33</citedby><cites>FETCH-LOGICAL-c555t-42fbf313f9db2b82f5fe92f3a94b3c9c490592004e3081b3c4ac95d74e8fcaa33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3845890$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3845890$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17752224$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16627745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>D'Angelo, Maximiliano A</creatorcontrib><creatorcontrib>Anderson, Daniel J</creatorcontrib><creatorcontrib>Richard, Erin</creatorcontrib><creatorcontrib>Hetzer, Martin W</creatorcontrib><title>Nuclear Pores Form de Novo from Both Sides of the Nuclear Envelope</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Nuclear pore complexes are multiprotein channels that span the double lipid bilayer of the nuclear envelope. How new pores are inserted into the intact nuclear envelope of proliferating and differentiating eukaryotic cells is unknown. We found that the Nup107-160 complex was incorporated into assembly sites in the nuclear envelope from both the nucleoplasmic and the cytoplasmic sides. Nuclear pore insertion required the generation of Ran guanosine triphosphate in the nuclear and cytoplasmic compartments. Newly formed nuclear pore complexes did not contain structural components of preexisting pores, suggesting that they can form de novo.</description><subject>Active Transport, Cell Nucleus</subject><subject>Animals</subject><subject>beta Karyopherins - metabolism</subject><subject>beta Karyopherins - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell lines</subject><subject>Cell membranes. Ionic channels. Membrane pores</subject><subject>Cell structures and functions</subject><subject>Cell-Free System</subject><subject>Cytoplasm - metabolism</subject><subject>Cytosol</subject><subject>Egtazic Acid - analogs & derivatives</subject><subject>Egtazic Acid - pharmacology</subject><subject>Eukaryotes</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guanosine Triphosphate - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted</subject><subject>Karyopherins</subject><subject>Lipids</subject><subject>Microscopy</subject><subject>Microscopy, Confocal</subject><subject>Molecular and cellular biology</subject><subject>Nuclear Envelope - metabolism</subject><subject>Nuclear Envelope - ultrastructure</subject><subject>Nuclear membrane</subject><subject>Nuclear pore</subject><subject>Nuclear Pore - metabolism</subject><subject>Nuclear Pore - ultrastructure</subject><subject>Nuclear pore complex proteins</subject><subject>Nuclear Pore Complex Proteins - metabolism</subject><subject>Proteins</subject><subject>ran GTP-Binding Protein - metabolism</subject><subject>Recombinant Proteins - metabolism</subject><subject>Signal detection</subject><subject>Spermatozoa</subject><subject>Wheat Germ Agglutinins - metabolism</subject><subject>Wheat Germ Agglutinins - pharmacology</subject><subject>Xenopus</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtr3DAUhUVpSCaPdTelNYVk5-TqZUnLzJBpAyEppFkLWZYaD7Y1kexA_301jGmgm6yEdL57uDoHoU8YLjEm1VWyrRus210YVtUHtMCgeKkI0I9oAUCrUoLgR-g4pQ1A1hQ9REe4qogQjC_Q8n6ynTOx-BmiS8U6xL5oXHEfXkPhY-iLZRifi8e2yWLwxfictXniZnh1Xdi6U3TgTZfc2XyeoKf1za_Vj_Lu4fvt6vqutJzzsWTE155i6lVTk1oSz71TxFOjWE2tskwBz3sDcxQkzk_MWMUbwZz01hhKT9DF3ncbw8vk0qj7NlnXdWZwYUq6ElIyLuBdkEjgUonqXRALTCmGHfjtP3ATpjjk32qCKZecEZKhqz1kY0gpOq-3se1N_KMx6F1bem5Lz23liS-z7VT3rnnj53oycD4DJlnT-WgG26Y3TghOCGGZ-7znNmkM8Z9Ocx5S7QL5upe9Cdr8jtni6ZEApoBBQM6d_gWrC644</recordid><startdate>20060421</startdate><enddate>20060421</enddate><creator>D'Angelo, Maximiliano A</creator><creator>Anderson, Daniel J</creator><creator>Richard, Erin</creator><creator>Hetzer, Martin W</creator><general>American Association for the Advancement of Science</general><general>The American Association for the Advancement of Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060421</creationdate><title>Nuclear Pores Form de Novo from Both Sides of the Nuclear Envelope</title><author>D'Angelo, Maximiliano A ; Anderson, Daniel J ; Richard, Erin ; Hetzer, Martin W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c555t-42fbf313f9db2b82f5fe92f3a94b3c9c490592004e3081b3c4ac95d74e8fcaa33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Active Transport, Cell Nucleus</topic><topic>Animals</topic><topic>beta Karyopherins - metabolism</topic><topic>beta Karyopherins - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell lines</topic><topic>Cell membranes. Ionic channels. Membrane pores</topic><topic>Cell structures and functions</topic><topic>Cell-Free System</topic><topic>Cytoplasm - metabolism</topic><topic>Cytosol</topic><topic>Egtazic Acid - analogs & derivatives</topic><topic>Egtazic Acid - pharmacology</topic><topic>Eukaryotes</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guanosine Triphosphate - metabolism</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted</topic><topic>Karyopherins</topic><topic>Lipids</topic><topic>Microscopy</topic><topic>Microscopy, Confocal</topic><topic>Molecular and cellular biology</topic><topic>Nuclear Envelope - metabolism</topic><topic>Nuclear Envelope - ultrastructure</topic><topic>Nuclear membrane</topic><topic>Nuclear pore</topic><topic>Nuclear Pore - metabolism</topic><topic>Nuclear Pore - ultrastructure</topic><topic>Nuclear pore complex proteins</topic><topic>Nuclear Pore Complex Proteins - metabolism</topic><topic>Proteins</topic><topic>ran GTP-Binding Protein - metabolism</topic><topic>Recombinant Proteins - metabolism</topic><topic>Signal detection</topic><topic>Spermatozoa</topic><topic>Wheat Germ Agglutinins - metabolism</topic><topic>Wheat Germ Agglutinins - pharmacology</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>D'Angelo, Maximiliano A</creatorcontrib><creatorcontrib>Anderson, Daniel J</creatorcontrib><creatorcontrib>Richard, Erin</creatorcontrib><creatorcontrib>Hetzer, Martin W</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>D'Angelo, Maximiliano A</au><au>Anderson, Daniel J</au><au>Richard, Erin</au><au>Hetzer, Martin W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear Pores Form de Novo from Both Sides of the Nuclear Envelope</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2006-04-21</date><risdate>2006</risdate><volume>312</volume><issue>5772</issue><spage>440</spage><epage>443</epage><pages>440-443</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Nuclear pore complexes are multiprotein channels that span the double lipid bilayer of the nuclear envelope. How new pores are inserted into the intact nuclear envelope of proliferating and differentiating eukaryotic cells is unknown. We found that the Nup107-160 complex was incorporated into assembly sites in the nuclear envelope from both the nucleoplasmic and the cytoplasmic sides. Nuclear pore insertion required the generation of Ran guanosine triphosphate in the nuclear and cytoplasmic compartments. Newly formed nuclear pore complexes did not contain structural components of preexisting pores, suggesting that they can form de novo.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>16627745</pmid><doi>10.1126/science.1124196</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2006-04, Vol.312 (5772), p.440-443 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67884570 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Active Transport, Cell Nucleus Animals beta Karyopherins - metabolism beta Karyopherins - pharmacology Biological and medical sciences Cell lines Cell membranes. Ionic channels. Membrane pores Cell structures and functions Cell-Free System Cytoplasm - metabolism Cytosol Egtazic Acid - analogs & derivatives Egtazic Acid - pharmacology Eukaryotes Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Guanosine Triphosphate - metabolism HeLa Cells Humans Image Processing, Computer-Assisted Karyopherins Lipids Microscopy Microscopy, Confocal Molecular and cellular biology Nuclear Envelope - metabolism Nuclear Envelope - ultrastructure Nuclear membrane Nuclear pore Nuclear Pore - metabolism Nuclear Pore - ultrastructure Nuclear pore complex proteins Nuclear Pore Complex Proteins - metabolism Proteins ran GTP-Binding Protein - metabolism Recombinant Proteins - metabolism Signal detection Spermatozoa Wheat Germ Agglutinins - metabolism Wheat Germ Agglutinins - pharmacology Xenopus |
| title | Nuclear Pores Form de Novo from Both Sides of the Nuclear Envelope |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T07%3A59%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nuclear%20Pores%20Form%20de%20Novo%20from%20Both%20Sides%20of%20the%20Nuclear%20Envelope&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=D'Angelo,%20Maximiliano%20A&rft.date=2006-04-21&rft.volume=312&rft.issue=5772&rft.spage=440&rft.epage=443&rft.pages=440-443&rft.issn=0036-8075&rft.eissn=1095-9203&rft.coden=SCIEAS&rft_id=info:doi/10.1126/science.1124196&rft_dat=%3Cjstor_proqu%3E3845890%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213585422&rft_id=info:pmid/16627745&rft_jstor_id=3845890&rfr_iscdi=true |