Probing the role of multicellular organization in three-dimensional microenvironments
Successful application of living cells in regenerative medicine requires an understanding of how tissue structure relates to organ function. There is growing evidence that presentation of extracellular cues in a three-dimensional (3D) context can fundamentally alter cellular responses. Thus, microen...
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| Veröffentlicht in: | Nature methods 2006-05, Vol.3 (5), p.369-375 |
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| creator | Bhatia, Sangeeta N Albrecht, Dirk R Underhill, Gregory H Wassermann, Travis B Sah, Robert L |
| description | Successful application of living cells in regenerative medicine requires an understanding of how tissue structure relates to organ function. There is growing evidence that presentation of extracellular cues in a three-dimensional (3D) context can fundamentally alter cellular responses. Thus, microenvironment studies that previously were limited to adherent two-dimensional (2D) cultures may not be appropriate for many cell types. Here we present a method for the rapid formation of reproducible, high-resolution 3D cellular structures within a photopolymerizable hydrogel using dielectrophoretic forces. We demonstrate the parallel formation of >20,000 cell clusters of precise size and shape within a thin 2-cm
2
hydrogel and the maintenance of high cell viability and differentiated cell markers over 2 weeks. By modulating cell-cell interactions in 3D clusters, we present the first evidence that microscale tissue organization regulates bovine articular chondrocyte biosynthesis. This platform permits investigation of tissue architecture in other multicellular processes, from embryogenesis to regeneration to tumorigenesis. |
| doi_str_mv | 10.1038/nmeth873 |
| format | Article |
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hydrogel and the maintenance of high cell viability and differentiated cell markers over 2 weeks. By modulating cell-cell interactions in 3D clusters, we present the first evidence that microscale tissue organization regulates bovine articular chondrocyte biosynthesis. This platform permits investigation of tissue architecture in other multicellular processes, from embryogenesis to regeneration to tumorigenesis.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/nmeth873</identifier><identifier>PMID: 16628207</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Animals ; Bioinformatics ; Biological Microscopy ; Biological Techniques ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biosensing Techniques - methods ; Biosynthesis ; Cartilage cells ; Cartilage, Articular - cytology ; Cartilage, Articular - pathology ; Cattle ; Cell culture ; Cell Culture Techniques - methods ; Cell Differentiation ; Cell interaction ; Cell Survival ; Cellular biology ; Chondrocytes - cytology ; Chondrocytes - pathology ; Dielectrics ; DNA probes ; Electromagnetic Fields ; Embryonic Development - physiology ; Embryonic growth stage ; Genetic aspects ; Hydrogels - chemistry ; Imaging, Three-Dimensional ; Life Sciences ; Methods ; Microenvironments ; Neoplasms - pathology ; Physiological aspects ; Proteomics ; Regeneration - physiology ; Time Factors ; Tissue Engineering ; Tissues ; Tumor Cells, Cultured - pathology</subject><ispartof>Nature methods, 2006-05, Vol.3 (5), p.369-375</ispartof><rights>Springer Nature America, Inc. 2006</rights><rights>COPYRIGHT 2006 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group May 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-4215850aec8f176c9b0d8b84c386d66ed12be068d0795241dc5cc7debf8d1b2e3</citedby><cites>FETCH-LOGICAL-c529t-4215850aec8f176c9b0d8b84c386d66ed12be068d0795241dc5cc7debf8d1b2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16628207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bhatia, Sangeeta N</creatorcontrib><creatorcontrib>Albrecht, Dirk R</creatorcontrib><creatorcontrib>Underhill, Gregory H</creatorcontrib><creatorcontrib>Wassermann, Travis B</creatorcontrib><creatorcontrib>Sah, Robert L</creatorcontrib><title>Probing the role of multicellular organization in three-dimensional microenvironments</title><title>Nature methods</title><addtitle>Nat Methods</addtitle><addtitle>Nat Methods</addtitle><description>Successful application of living cells in regenerative medicine requires an understanding of how tissue structure relates to organ function. There is growing evidence that presentation of extracellular cues in a three-dimensional (3D) context can fundamentally alter cellular responses. Thus, microenvironment studies that previously were limited to adherent two-dimensional (2D) cultures may not be appropriate for many cell types. Here we present a method for the rapid formation of reproducible, high-resolution 3D cellular structures within a photopolymerizable hydrogel using dielectrophoretic forces. We demonstrate the parallel formation of >20,000 cell clusters of precise size and shape within a thin 2-cm
2
hydrogel and the maintenance of high cell viability and differentiated cell markers over 2 weeks. By modulating cell-cell interactions in 3D clusters, we present the first evidence that microscale tissue organization regulates bovine articular chondrocyte biosynthesis. This platform permits investigation of tissue architecture in other multicellular processes, from embryogenesis to regeneration to tumorigenesis.</description><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biosensing Techniques - methods</subject><subject>Biosynthesis</subject><subject>Cartilage cells</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - pathology</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell interaction</subject><subject>Cell Survival</subject><subject>Cellular biology</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - pathology</subject><subject>Dielectrics</subject><subject>DNA probes</subject><subject>Electromagnetic Fields</subject><subject>Embryonic Development - 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Academic</collection><jtitle>Nature methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhatia, Sangeeta N</au><au>Albrecht, Dirk R</au><au>Underhill, Gregory H</au><au>Wassermann, Travis B</au><au>Sah, Robert L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Probing the role of multicellular organization in three-dimensional microenvironments</atitle><jtitle>Nature methods</jtitle><stitle>Nat Methods</stitle><addtitle>Nat Methods</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>3</volume><issue>5</issue><spage>369</spage><epage>375</epage><pages>369-375</pages><issn>1548-7091</issn><eissn>1548-7105</eissn><abstract>Successful application of living cells in regenerative medicine requires an understanding of how tissue structure relates to organ function. There is growing evidence that presentation of extracellular cues in a three-dimensional (3D) context can fundamentally alter cellular responses. Thus, microenvironment studies that previously were limited to adherent two-dimensional (2D) cultures may not be appropriate for many cell types. Here we present a method for the rapid formation of reproducible, high-resolution 3D cellular structures within a photopolymerizable hydrogel using dielectrophoretic forces. We demonstrate the parallel formation of >20,000 cell clusters of precise size and shape within a thin 2-cm
2
hydrogel and the maintenance of high cell viability and differentiated cell markers over 2 weeks. By modulating cell-cell interactions in 3D clusters, we present the first evidence that microscale tissue organization regulates bovine articular chondrocyte biosynthesis. This platform permits investigation of tissue architecture in other multicellular processes, from embryogenesis to regeneration to tumorigenesis.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>16628207</pmid><doi>10.1038/nmeth873</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biosensing Techniques - methods Biosynthesis Cartilage cells Cartilage, Articular - cytology Cartilage, Articular - pathology Cattle Cell culture Cell Culture Techniques - methods Cell Differentiation Cell interaction Cell Survival Cellular biology Chondrocytes - cytology Chondrocytes - pathology Dielectrics DNA probes Electromagnetic Fields Embryonic Development - physiology Embryonic growth stage Genetic aspects Hydrogels - chemistry Imaging, Three-Dimensional Life Sciences Methods Microenvironments Neoplasms - pathology Physiological aspects Proteomics Regeneration - physiology Time Factors Tissue Engineering Tissues Tumor Cells, Cultured - pathology |
| title | Probing the role of multicellular organization in three-dimensional microenvironments |
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