Characterisation of protein and antigen variability among Mycoplasma mycoides subsp. mycoides (LC) and Mycoplasma agalactiae field strains by SDS-PAGE and immunoblotting
Mycoplasma mycoides subsp. mycoides (LC) ( Mmm LC) and Mycoplasma agalactiae are the most important mycoplasma species involved in the contagious agalactia syndrome. A total of 25 field strains from Spain and the two type strains were analysed by SDS-PAGE and immunoblotting. Two polyclonal antisera...
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| Veröffentlicht in: | The veterinary journal (1997) 2006-05, Vol.171 (3), p.532-538 |
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| container_title | The veterinary journal (1997) |
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| creator | Fe, C. de la Assunção, P. Rosales, R.S. Antunes, T. Poveda, J.B. |
| description | Mycoplasma mycoides subsp.
mycoides (LC) (
Mmm LC) and
Mycoplasma agalactiae are the most important mycoplasma species involved in the contagious agalactia syndrome. A total of 25 field strains from Spain and the two type strains were analysed by SDS-PAGE and immunoblotting. Two polyclonal antisera (PAbs) raised against a pool of strains of each mycoplasma species were used. The results revealed a high degree of protein variability among the field strains. The type strain of
Mmm LC appeared to be representative of the field strains of this species, whereas this was not the case with the
M. agalactiae type strain. Whereas
M. agalactiae is known to possess a gene family regulating surface antigen diversity, there is a need to study the mechanisms used by
Mmm LC to generate antigenic variability in more detail. |
| doi_str_mv | 10.1016/j.tvjl.2005.02.015 |
| format | Article |
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mycoides (LC) (
Mmm LC) and
Mycoplasma agalactiae are the most important mycoplasma species involved in the contagious agalactia syndrome. A total of 25 field strains from Spain and the two type strains were analysed by SDS-PAGE and immunoblotting. Two polyclonal antisera (PAbs) raised against a pool of strains of each mycoplasma species were used. The results revealed a high degree of protein variability among the field strains. The type strain of
Mmm LC appeared to be representative of the field strains of this species, whereas this was not the case with the
M. agalactiae type strain. Whereas
M. agalactiae is known to possess a gene family regulating surface antigen diversity, there is a need to study the mechanisms used by
Mmm LC to generate antigenic variability in more detail.</description><identifier>ISSN: 1090-0233</identifier><identifier>EISSN: 1532-2971</identifier><identifier>DOI: 10.1016/j.tvjl.2005.02.015</identifier><identifier>PMID: 16624721</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>animal pathogenic bacteria ; Animals ; Antigenic Variation ; bacterial antigens ; bacterial proteins ; Contagious agalactia ; contagious agalactia syndrome ; Electrophoresis, Polyacrylamide Gel - veterinary ; Immunoblotting ; Immunoblotting - veterinary ; Mycoplasma agalactiae ; Mycoplasma agalactiae - classification ; Mycoplasma agalactiae - genetics ; Mycoplasma Infections - microbiology ; Mycoplasma Infections - veterinary ; Mycoplasma mycoides - classification ; Mycoplasma mycoides - genetics ; Mycoplasma mycoides subsp. mycoides ; Mycoplasma mycoides subsp. mycoides LC ; mycoplasmosis ; Phylogeny ; Pleuropneumonia, Contagious - microbiology ; polyacrylamide gel electrophoresis ; polyclonal antibodies ; SDS-PAGE ; Species Specificity ; strain differences ; strains ; surface antigens</subject><ispartof>The veterinary journal (1997), 2006-05, Vol.171 (3), p.532-538</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-d336e28fc15af01dc0dbcb09ee109bc78becea0bb454d6c609c5dc4b8f9b2be33</citedby><cites>FETCH-LOGICAL-c378t-d336e28fc15af01dc0dbcb09ee109bc78becea0bb454d6c609c5dc4b8f9b2be33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tvjl.2005.02.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16624721$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fe, C. de la</creatorcontrib><creatorcontrib>Assunção, P.</creatorcontrib><creatorcontrib>Rosales, R.S.</creatorcontrib><creatorcontrib>Antunes, T.</creatorcontrib><creatorcontrib>Poveda, J.B.</creatorcontrib><title>Characterisation of protein and antigen variability among Mycoplasma mycoides subsp. mycoides (LC) and Mycoplasma agalactiae field strains by SDS-PAGE and immunoblotting</title><title>The veterinary journal (1997)</title><addtitle>Vet J</addtitle><description>Mycoplasma mycoides subsp.
mycoides (LC) (
Mmm LC) and
Mycoplasma agalactiae are the most important mycoplasma species involved in the contagious agalactia syndrome. A total of 25 field strains from Spain and the two type strains were analysed by SDS-PAGE and immunoblotting. Two polyclonal antisera (PAbs) raised against a pool of strains of each mycoplasma species were used. The results revealed a high degree of protein variability among the field strains. The type strain of
Mmm LC appeared to be representative of the field strains of this species, whereas this was not the case with the
M. agalactiae type strain. Whereas
M. agalactiae is known to possess a gene family regulating surface antigen diversity, there is a need to study the mechanisms used by
Mmm LC to generate antigenic variability in more detail.</description><subject>animal pathogenic bacteria</subject><subject>Animals</subject><subject>Antigenic Variation</subject><subject>bacterial antigens</subject><subject>bacterial proteins</subject><subject>Contagious agalactia</subject><subject>contagious agalactia syndrome</subject><subject>Electrophoresis, Polyacrylamide Gel - veterinary</subject><subject>Immunoblotting</subject><subject>Immunoblotting - veterinary</subject><subject>Mycoplasma agalactiae</subject><subject>Mycoplasma agalactiae - classification</subject><subject>Mycoplasma agalactiae - genetics</subject><subject>Mycoplasma Infections - microbiology</subject><subject>Mycoplasma Infections - veterinary</subject><subject>Mycoplasma mycoides - classification</subject><subject>Mycoplasma mycoides - genetics</subject><subject>Mycoplasma mycoides subsp. mycoides</subject><subject>Mycoplasma mycoides subsp. mycoides LC</subject><subject>mycoplasmosis</subject><subject>Phylogeny</subject><subject>Pleuropneumonia, Contagious - microbiology</subject><subject>polyacrylamide gel electrophoresis</subject><subject>polyclonal antibodies</subject><subject>SDS-PAGE</subject><subject>Species Specificity</subject><subject>strain differences</subject><subject>strains</subject><subject>surface antigens</subject><issn>1090-0233</issn><issn>1532-2971</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV-L1DAUxYso7rr6BXzQPIk-tN4kbacFX5ZxXYURhXGfQ_7c1gxtM5ukA_OR_JamOwPrk4GQG_idk3BOlr2mUFCg9cddEQ-7oWAAVQGsAFo9yS5pxVnO2hV9mmZoIQfG-UX2IoQdALRlyZ5nF7SuWbli9DL7s_4tvdQRvQ0yWjcR15G9dxHtRORk0o62x4kcpLdS2cHGI5Gjm3ry_ajdfpBhlGRMozUYSJhV2BeP9_eb9YcHm39g2cshvWglks7iYEiIXtopEHUk28_b_Of17c2Dxo7jPDk1uBjt1L_MnnVyCPjqfF5ld19ufq2_5psft9_W15tc81UTc8N5jazpNK1kB9RoMEoraBFTGkqvGoUaJShVVqWpdQ2trowuVdO1iink_Cp7d_JNKdzPGKIYbdA4DHJCNwdRr5qGc7qA7ARq70Lw2Im9t6P0R0FBLAWJnVgKEktBAphIBSXRm7P7rEY0j5JzIwl4ewI66YTsUy3ibsuAcqDLqlgiPp0ITCkcLHoRtMVJo7EedRTG2f_94C9NOq-T</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Fe, C. de la</creator><creator>Assunção, P.</creator><creator>Rosales, R.S.</creator><creator>Antunes, T.</creator><creator>Poveda, J.B.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>Characterisation of protein and antigen variability among Mycoplasma mycoides subsp. mycoides (LC) and Mycoplasma agalactiae field strains by SDS-PAGE and immunoblotting</title><author>Fe, C. de la ; Assunção, P. ; Rosales, R.S. ; Antunes, T. ; Poveda, J.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-d336e28fc15af01dc0dbcb09ee109bc78becea0bb454d6c609c5dc4b8f9b2be33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>animal pathogenic bacteria</topic><topic>Animals</topic><topic>Antigenic Variation</topic><topic>bacterial antigens</topic><topic>bacterial proteins</topic><topic>Contagious agalactia</topic><topic>contagious agalactia syndrome</topic><topic>Electrophoresis, Polyacrylamide Gel - veterinary</topic><topic>Immunoblotting</topic><topic>Immunoblotting - veterinary</topic><topic>Mycoplasma agalactiae</topic><topic>Mycoplasma agalactiae - classification</topic><topic>Mycoplasma agalactiae - genetics</topic><topic>Mycoplasma Infections - microbiology</topic><topic>Mycoplasma Infections - veterinary</topic><topic>Mycoplasma mycoides - classification</topic><topic>Mycoplasma mycoides - genetics</topic><topic>Mycoplasma mycoides subsp. mycoides</topic><topic>Mycoplasma mycoides subsp. mycoides LC</topic><topic>mycoplasmosis</topic><topic>Phylogeny</topic><topic>Pleuropneumonia, Contagious - microbiology</topic><topic>polyacrylamide gel electrophoresis</topic><topic>polyclonal antibodies</topic><topic>SDS-PAGE</topic><topic>Species Specificity</topic><topic>strain differences</topic><topic>strains</topic><topic>surface antigens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fe, C. de la</creatorcontrib><creatorcontrib>Assunção, P.</creatorcontrib><creatorcontrib>Rosales, R.S.</creatorcontrib><creatorcontrib>Antunes, T.</creatorcontrib><creatorcontrib>Poveda, J.B.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The veterinary journal (1997)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fe, C. de la</au><au>Assunção, P.</au><au>Rosales, R.S.</au><au>Antunes, T.</au><au>Poveda, J.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of protein and antigen variability among Mycoplasma mycoides subsp. mycoides (LC) and Mycoplasma agalactiae field strains by SDS-PAGE and immunoblotting</atitle><jtitle>The veterinary journal (1997)</jtitle><addtitle>Vet J</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>171</volume><issue>3</issue><spage>532</spage><epage>538</epage><pages>532-538</pages><issn>1090-0233</issn><eissn>1532-2971</eissn><abstract>Mycoplasma mycoides subsp.
mycoides (LC) (
Mmm LC) and
Mycoplasma agalactiae are the most important mycoplasma species involved in the contagious agalactia syndrome. A total of 25 field strains from Spain and the two type strains were analysed by SDS-PAGE and immunoblotting. Two polyclonal antisera (PAbs) raised against a pool of strains of each mycoplasma species were used. The results revealed a high degree of protein variability among the field strains. The type strain of
Mmm LC appeared to be representative of the field strains of this species, whereas this was not the case with the
M. agalactiae type strain. Whereas
M. agalactiae is known to possess a gene family regulating surface antigen diversity, there is a need to study the mechanisms used by
Mmm LC to generate antigenic variability in more detail.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16624721</pmid><doi>10.1016/j.tvjl.2005.02.015</doi><tpages>7</tpages></addata></record> |
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| subjects | animal pathogenic bacteria Animals Antigenic Variation bacterial antigens bacterial proteins Contagious agalactia contagious agalactia syndrome Electrophoresis, Polyacrylamide Gel - veterinary Immunoblotting Immunoblotting - veterinary Mycoplasma agalactiae Mycoplasma agalactiae - classification Mycoplasma agalactiae - genetics Mycoplasma Infections - microbiology Mycoplasma Infections - veterinary Mycoplasma mycoides - classification Mycoplasma mycoides - genetics Mycoplasma mycoides subsp. mycoides Mycoplasma mycoides subsp. mycoides LC mycoplasmosis Phylogeny Pleuropneumonia, Contagious - microbiology polyacrylamide gel electrophoresis polyclonal antibodies SDS-PAGE Species Specificity strain differences strains surface antigens |
| title | Characterisation of protein and antigen variability among Mycoplasma mycoides subsp. mycoides (LC) and Mycoplasma agalactiae field strains by SDS-PAGE and immunoblotting |
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