Rapid determination of trace amounts of minoxidil in hamster skin follicles with various formulations using narrow-bore LC/EC
A sensitive liquid chromatographic method with electrochemistry (LC/EC) was developed for the determination of trace of minoxidil in hamster skin follicles after topical administration of the ear using various formulations. The minoxidil in the sebaceous glands of the hamster ear was isolated from t...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2005-07, Vol.38 (3), p.532-536 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Huang, Tiehua Garceau, Michelle E. Ramstad, Tore Stehle, Randall G. |
| description | A sensitive liquid chromatographic method with electrochemistry (LC/EC) was developed for the determination of trace of minoxidil in hamster skin follicles after topical administration of the ear using various formulations. The minoxidil in the sebaceous glands of the hamster ear was isolated from the skin and the follicles in different skin layers were treated with aqueous trichloroacetic acid followed by acetonitrile. The supernatant was directly injected into the LC/EC system and minoxidil was detected by oxidation at +800
mV versus Ag/AgCl using a glassy carbon electrode. The analytical recoveries were between 94.4 and103.1% and the linearity was excellent up to 250
μg/ml with a regression coefficient (
r
2) of 0.9988. The LC/EC and the widely used radiolabeled scintillation methods agree well and both show high sensitivities. The LC/EC method is rapid and cost-effective with a detection limit of only 1
ng/ml. |
| doi_str_mv | 10.1016/j.jpba.2005.01.032 |
| format | Article |
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mV versus Ag/AgCl using a glassy carbon electrode. The analytical recoveries were between 94.4 and103.1% and the linearity was excellent up to 250
μg/ml with a regression coefficient (
r
2) of 0.9988. The LC/EC and the widely used radiolabeled scintillation methods agree well and both show high sensitivities. The LC/EC method is rapid and cost-effective with a detection limit of only 1
ng/ml.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2005.01.032</identifier><identifier>PMID: 15925256</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Cricetinae ; Dermis - metabolism ; Dosage Forms ; Ear ; Electrochemical detection ; Electrochemistry - methods ; Follicles ; Formulation ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Hydrogen-Ion Concentration ; Medical sciences ; Microbore liquid chromatography ; Minoxidil ; Minoxidil - analysis ; Minoxidil - isolation & purification ; Minoxidil - pharmacokinetics ; Pharmacology. Drug treatments ; Polyethylene Glycols - chemistry ; Reproducibility of Results ; Sebaceous Glands - metabolism ; Solubility ; Solvents - chemistry ; Trichloroacetic Acid - chemistry</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2005-07, Vol.38 (3), p.532-536</ispartof><rights>2005</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-2fbcfec4706ae5a9cb209d8e0733b6e81ff238af1f0a292391ca5a7113754b683</citedby><cites>FETCH-LOGICAL-c384t-2fbcfec4706ae5a9cb209d8e0733b6e81ff238af1f0a292391ca5a7113754b683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2005.01.032$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16860845$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15925256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Tiehua</creatorcontrib><creatorcontrib>Garceau, Michelle E.</creatorcontrib><creatorcontrib>Ramstad, Tore</creatorcontrib><creatorcontrib>Stehle, Randall G.</creatorcontrib><title>Rapid determination of trace amounts of minoxidil in hamster skin follicles with various formulations using narrow-bore LC/EC</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A sensitive liquid chromatographic method with electrochemistry (LC/EC) was developed for the determination of trace of minoxidil in hamster skin follicles after topical administration of the ear using various formulations. The minoxidil in the sebaceous glands of the hamster ear was isolated from the skin and the follicles in different skin layers were treated with aqueous trichloroacetic acid followed by acetonitrile. The supernatant was directly injected into the LC/EC system and minoxidil was detected by oxidation at +800
mV versus Ag/AgCl using a glassy carbon electrode. The analytical recoveries were between 94.4 and103.1% and the linearity was excellent up to 250
μg/ml with a regression coefficient (
r
2) of 0.9988. The LC/EC and the widely used radiolabeled scintillation methods agree well and both show high sensitivities. The LC/EC method is rapid and cost-effective with a detection limit of only 1
ng/ml.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cricetinae</subject><subject>Dermis - metabolism</subject><subject>Dosage Forms</subject><subject>Ear</subject><subject>Electrochemical detection</subject><subject>Electrochemistry - methods</subject><subject>Follicles</subject><subject>Formulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Medical sciences</subject><subject>Microbore liquid chromatography</subject><subject>Minoxidil</subject><subject>Minoxidil - analysis</subject><subject>Minoxidil - isolation & purification</subject><subject>Minoxidil - pharmacokinetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Reproducibility of Results</subject><subject>Sebaceous Glands - metabolism</subject><subject>Solubility</subject><subject>Solvents - chemistry</subject><subject>Trichloroacetic Acid - chemistry</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE-L1DAYh4Mo7rj6BTxILu6t3SRt0hS8yLD-gQFBFLyFt-kbN2PajEm7qwe_uxlnYG-eQt48vx9vHkJeclZzxtX1vt4fBqgFY7JmvGaNeEQ2XHdNJVT77THZsK7hVce0vCDPct6zAvK-fUouuOyFFFJtyJ_PcPAjHXHBNPkZFh9nGh1dElikMMV1XvJxUB7jLz_6QP1Mb2HKJUDzj3JxMQRvA2Z675dbegfJxzWXcZrW8K8w0zX7-TudIaV4Xw0xId1tr2-2z8kTByHji_N5Sb6-u_my_VDtPr3_uH27q2yj26USbrAObdsxBSiht4Ng_aix_K8ZFGrunGg0OO4YiF40PbcgoeO86WQ7KN1ckqtT7yHFnyvmxUw-WwwBZiy7GtVpLXqpCihOoE0x54TOHJKfIP02nJmjdLM3R-nmKN0wbor0Enp1bl-HCceHyNlyAV6fAcgWgkswW58fOKUV060s3JsTh8XFncdksvU4Wxx9QruYMfr_7fEXozWiTA</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>Huang, Tiehua</creator><creator>Garceau, Michelle E.</creator><creator>Ramstad, Tore</creator><creator>Stehle, Randall G.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050701</creationdate><title>Rapid determination of trace amounts of minoxidil in hamster skin follicles with various formulations using narrow-bore LC/EC</title><author>Huang, Tiehua ; Garceau, Michelle E. ; Ramstad, Tore ; Stehle, Randall G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-2fbcfec4706ae5a9cb209d8e0733b6e81ff238af1f0a292391ca5a7113754b683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cricetinae</topic><topic>Dermis - metabolism</topic><topic>Dosage Forms</topic><topic>Ear</topic><topic>Electrochemical detection</topic><topic>Electrochemistry - methods</topic><topic>Follicles</topic><topic>Formulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Medical sciences</topic><topic>Microbore liquid chromatography</topic><topic>Minoxidil</topic><topic>Minoxidil - analysis</topic><topic>Minoxidil - isolation & purification</topic><topic>Minoxidil - pharmacokinetics</topic><topic>Pharmacology. Drug treatments</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Reproducibility of Results</topic><topic>Sebaceous Glands - metabolism</topic><topic>Solubility</topic><topic>Solvents - chemistry</topic><topic>Trichloroacetic Acid - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Tiehua</creatorcontrib><creatorcontrib>Garceau, Michelle E.</creatorcontrib><creatorcontrib>Ramstad, Tore</creatorcontrib><creatorcontrib>Stehle, Randall G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Tiehua</au><au>Garceau, Michelle E.</au><au>Ramstad, Tore</au><au>Stehle, Randall G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid determination of trace amounts of minoxidil in hamster skin follicles with various formulations using narrow-bore LC/EC</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2005-07-01</date><risdate>2005</risdate><volume>38</volume><issue>3</issue><spage>532</spage><epage>536</epage><pages>532-536</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A sensitive liquid chromatographic method with electrochemistry (LC/EC) was developed for the determination of trace of minoxidil in hamster skin follicles after topical administration of the ear using various formulations. The minoxidil in the sebaceous glands of the hamster ear was isolated from the skin and the follicles in different skin layers were treated with aqueous trichloroacetic acid followed by acetonitrile. The supernatant was directly injected into the LC/EC system and minoxidil was detected by oxidation at +800
mV versus Ag/AgCl using a glassy carbon electrode. The analytical recoveries were between 94.4 and103.1% and the linearity was excellent up to 250
μg/ml with a regression coefficient (
r
2) of 0.9988. The LC/EC and the widely used radiolabeled scintillation methods agree well and both show high sensitivities. The LC/EC method is rapid and cost-effective with a detection limit of only 1
ng/ml.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15925256</pmid><doi>10.1016/j.jpba.2005.01.032</doi><tpages>5</tpages></addata></record> |
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| subjects | Analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid - methods Cricetinae Dermis - metabolism Dosage Forms Ear Electrochemical detection Electrochemistry - methods Follicles Formulation Fundamental and applied biological sciences. Psychology General pharmacology Hydrogen-Ion Concentration Medical sciences Microbore liquid chromatography Minoxidil Minoxidil - analysis Minoxidil - isolation & purification Minoxidil - pharmacokinetics Pharmacology. Drug treatments Polyethylene Glycols - chemistry Reproducibility of Results Sebaceous Glands - metabolism Solubility Solvents - chemistry Trichloroacetic Acid - chemistry |
| title | Rapid determination of trace amounts of minoxidil in hamster skin follicles with various formulations using narrow-bore LC/EC |
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