Gene expression of Corynebacterium glutamicum in response to the conditions inducing glutamate overproduction
The ultimate aim is to elucidate the molecular mechanisms for glutamate overproduction by Corynebacterium glutamicum. Gene expression in response to the conditions inducing glutamate overproduction was investigated by using a DNA microarray technique. Most genes involved in the EMP pathway, the PPP,...
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| Veröffentlicht in: | Letters in applied microbiology 2006-05, Vol.42 (5), p.471-476 |
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| creator | Kataoka, M Hashimoto, K.-I Yoshida, M Nakamatsu, T Horinouchi, S Kawasaki, H |
| description | The ultimate aim is to elucidate the molecular mechanisms for glutamate overproduction by Corynebacterium glutamicum. Gene expression in response to the conditions inducing glutamate overproduction was investigated by using a DNA microarray technique. Most genes involved in the EMP pathway, the PPP, and the TCA cycle were downregulated, while five genes that were highly upregulated (NCgl0917, NCgl2944, NCgl2945, NCgl2946, and NCgl2975) were identified under all the three conditions for overproduction that are studied here. Gene products of NCgl2944, NCgl2945, and NCgl2946 were highly homologous to each other, did not resemble any other protein, and have remained uncharacterized thus far. The product of NCgl0917 showed a similarity to a few hypothetical and uncharacterized proteins. NCgl2975 was homologous to metal-binding proteins. The decrease in the activity of 2-oxoglutarate dehydrogenase complex, a key enzyme that is downregulated during glutamate overproduction, can be mainly attributed to the downregulation of odhA and sucB. Five highly upregulated genes were also identified. Although fermentative production of glutamate has been carried out for more than 45 years, information on the molecular mechanisms of glutamate overproduction is still limited. This study further elucidates these mechanisms. |
| doi_str_mv | 10.1111/j.1472-765X.2006.01905.x |
| format | Article |
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Gene expression in response to the conditions inducing glutamate overproduction was investigated by using a DNA microarray technique. Most genes involved in the EMP pathway, the PPP, and the TCA cycle were downregulated, while five genes that were highly upregulated (NCgl0917, NCgl2944, NCgl2945, NCgl2946, and NCgl2975) were identified under all the three conditions for overproduction that are studied here. Gene products of NCgl2944, NCgl2945, and NCgl2946 were highly homologous to each other, did not resemble any other protein, and have remained uncharacterized thus far. The product of NCgl0917 showed a similarity to a few hypothetical and uncharacterized proteins. NCgl2975 was homologous to metal-binding proteins. The decrease in the activity of 2-oxoglutarate dehydrogenase complex, a key enzyme that is downregulated during glutamate overproduction, can be mainly attributed to the downregulation of odhA and sucB. Five highly upregulated genes were also identified. Although fermentative production of glutamate has been carried out for more than 45 years, information on the molecular mechanisms of glutamate overproduction is still limited. This study further elucidates these mechanisms.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>EISSN: 1365-2673</identifier><identifier>DOI: 10.1111/j.1472-765X.2006.01905.x</identifier><identifier>PMID: 16620205</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>amino acid ; Biological and medical sciences ; Citric Acid Cycle - genetics ; Corynebacterium glutamicum ; Corynebacterium glutamicum - genetics ; Corynebacterium glutamicum - metabolism ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; glutamate overproduction ; Glutamic Acid - biosynthesis ; Glycolysis - genetics ; Microbiology ; Oligonucleotide Array Sequence Analysis ; Pentose Phosphate Pathway - genetics ; Sequence Homology ; transcriptome analysis</subject><ispartof>Letters in applied microbiology, 2006-05, Vol.42 (5), p.471-476</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5685-32ee264fa62358385a39f16b7d0ab9bd578cc1caf640aa6ceda8729b119bf5603</citedby><cites>FETCH-LOGICAL-c5685-32ee264fa62358385a39f16b7d0ab9bd578cc1caf640aa6ceda8729b119bf5603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1472-765X.2006.01905.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1472-765X.2006.01905.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17676085$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16620205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kataoka, M</creatorcontrib><creatorcontrib>Hashimoto, K.-I</creatorcontrib><creatorcontrib>Yoshida, M</creatorcontrib><creatorcontrib>Nakamatsu, T</creatorcontrib><creatorcontrib>Horinouchi, S</creatorcontrib><creatorcontrib>Kawasaki, H</creatorcontrib><title>Gene expression of Corynebacterium glutamicum in response to the conditions inducing glutamate overproduction</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>The ultimate aim is to elucidate the molecular mechanisms for glutamate overproduction by Corynebacterium glutamicum. Gene expression in response to the conditions inducing glutamate overproduction was investigated by using a DNA microarray technique. Most genes involved in the EMP pathway, the PPP, and the TCA cycle were downregulated, while five genes that were highly upregulated (NCgl0917, NCgl2944, NCgl2945, NCgl2946, and NCgl2975) were identified under all the three conditions for overproduction that are studied here. Gene products of NCgl2944, NCgl2945, and NCgl2946 were highly homologous to each other, did not resemble any other protein, and have remained uncharacterized thus far. The product of NCgl0917 showed a similarity to a few hypothetical and uncharacterized proteins. NCgl2975 was homologous to metal-binding proteins. The decrease in the activity of 2-oxoglutarate dehydrogenase complex, a key enzyme that is downregulated during glutamate overproduction, can be mainly attributed to the downregulation of odhA and sucB. Five highly upregulated genes were also identified. Although fermentative production of glutamate has been carried out for more than 45 years, information on the molecular mechanisms of glutamate overproduction is still limited. This study further elucidates these mechanisms.</description><subject>amino acid</subject><subject>Biological and medical sciences</subject><subject>Citric Acid Cycle - genetics</subject><subject>Corynebacterium glutamicum</subject><subject>Corynebacterium glutamicum - genetics</subject><subject>Corynebacterium glutamicum - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>glutamate overproduction</subject><subject>Glutamic Acid - biosynthesis</subject><subject>Glycolysis - genetics</subject><subject>Microbiology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Pentose Phosphate Pathway - genetics</subject><subject>Sequence Homology</subject><subject>transcriptome analysis</subject><issn>0266-8254</issn><issn>1472-765X</issn><issn>1365-2673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-P0zAQxSMEYsvCVwBf4JYwduI_OXBYVbAgFXGAlbhZjjMprpK42MnSfnscGrFH8MUjz-_Ns-ZlGaFQ0HTeHgpaSZZLwb8XDEAUQGvgxelRtvnbeJxtgAmRK8arq-xZjAcAUJTVT7MrKgQDBnyTDbc4IsHTMWCMzo_Ed2Trw3nExtgJg5sHsu_nyQzOptKNJIFHP0YkkyfTDyTWj62bkjSmbjtbN-5XhZmQ-HsMx-DT-4I8z550po_4Yr2vs7sP779tP-a7L7eftje73HKheF4yRCaqzghWclUqbsq6o6KRLZimbloulbXUmk5UYIyw2BolWd1QWjcdF1BeZ28uc5P1zxnjpAcXLfa9GdHPUQupZC14_U-QSgayggVUF9AGH2PATh-DG0w4awp6yUQf9LJ6vaxeL5noP5noU5K-XD3mZsD2QbiGkIDXK2CiNX0XzGhdfOCkkALUwr27cL9cj-f__oDe3XxeqqR_ddF3xmuzD8nj7isDWgIFoVQpyt9aWrOw</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Kataoka, M</creator><creator>Hashimoto, K.-I</creator><creator>Yoshida, M</creator><creator>Nakamatsu, T</creator><creator>Horinouchi, S</creator><creator>Kawasaki, H</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200605</creationdate><title>Gene expression of Corynebacterium glutamicum in response to the conditions inducing glutamate overproduction</title><author>Kataoka, M ; Hashimoto, K.-I ; Yoshida, M ; Nakamatsu, T ; Horinouchi, S ; Kawasaki, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5685-32ee264fa62358385a39f16b7d0ab9bd578cc1caf640aa6ceda8729b119bf5603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>amino acid</topic><topic>Biological and medical sciences</topic><topic>Citric Acid Cycle - genetics</topic><topic>Corynebacterium glutamicum</topic><topic>Corynebacterium glutamicum - genetics</topic><topic>Corynebacterium glutamicum - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial</topic><topic>glutamate overproduction</topic><topic>Glutamic Acid - biosynthesis</topic><topic>Glycolysis - genetics</topic><topic>Microbiology</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Pentose Phosphate Pathway - genetics</topic><topic>Sequence Homology</topic><topic>transcriptome analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kataoka, M</creatorcontrib><creatorcontrib>Hashimoto, K.-I</creatorcontrib><creatorcontrib>Yoshida, M</creatorcontrib><creatorcontrib>Nakamatsu, T</creatorcontrib><creatorcontrib>Horinouchi, S</creatorcontrib><creatorcontrib>Kawasaki, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kataoka, M</au><au>Hashimoto, K.-I</au><au>Yoshida, M</au><au>Nakamatsu, T</au><au>Horinouchi, S</au><au>Kawasaki, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression of Corynebacterium glutamicum in response to the conditions inducing glutamate overproduction</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2006-05</date><risdate>2006</risdate><volume>42</volume><issue>5</issue><spage>471</spage><epage>476</epage><pages>471-476</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><eissn>1365-2673</eissn><coden>LAMIE7</coden><abstract>The ultimate aim is to elucidate the molecular mechanisms for glutamate overproduction by Corynebacterium glutamicum. Gene expression in response to the conditions inducing glutamate overproduction was investigated by using a DNA microarray technique. Most genes involved in the EMP pathway, the PPP, and the TCA cycle were downregulated, while five genes that were highly upregulated (NCgl0917, NCgl2944, NCgl2945, NCgl2946, and NCgl2975) were identified under all the three conditions for overproduction that are studied here. Gene products of NCgl2944, NCgl2945, and NCgl2946 were highly homologous to each other, did not resemble any other protein, and have remained uncharacterized thus far. The product of NCgl0917 showed a similarity to a few hypothetical and uncharacterized proteins. NCgl2975 was homologous to metal-binding proteins. The decrease in the activity of 2-oxoglutarate dehydrogenase complex, a key enzyme that is downregulated during glutamate overproduction, can be mainly attributed to the downregulation of odhA and sucB. Five highly upregulated genes were also identified. Although fermentative production of glutamate has been carried out for more than 45 years, information on the molecular mechanisms of glutamate overproduction is still limited. This study further elucidates these mechanisms.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>16620205</pmid><doi>10.1111/j.1472-765X.2006.01905.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | amino acid Biological and medical sciences Citric Acid Cycle - genetics Corynebacterium glutamicum Corynebacterium glutamicum - genetics Corynebacterium glutamicum - metabolism Fundamental and applied biological sciences. Psychology gene expression Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Bacterial glutamate overproduction Glutamic Acid - biosynthesis Glycolysis - genetics Microbiology Oligonucleotide Array Sequence Analysis Pentose Phosphate Pathway - genetics Sequence Homology transcriptome analysis |
| title | Gene expression of Corynebacterium glutamicum in response to the conditions inducing glutamate overproduction |
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