Sir2 and the Acetyltransferase, Pat, Regulate the Archaeal Chromatin Protein, Alba
The DNA binding affinity of Alba, a chromatin protein of the archaeon Sulfolobus solfataricus P2, is regulated by acetylation of lysine 16. Here we identify an acetyltransferase that specifically acetylates Alba on this residue. The effect of acetylation is to lower the affinity of Alba for DNA. Rem...
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| Veröffentlicht in: | The Journal of biological chemistry 2005-06, Vol.280 (22), p.21122-21128 |
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| container_end_page | 21128 |
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| container_issue | 22 |
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| container_title | The Journal of biological chemistry |
| container_volume | 280 |
| creator | Marsh, Victoria L. Peak-Chew, Sew Yeu Bell, Stephen D. |
| description | The DNA binding affinity of Alba, a chromatin protein of the archaeon Sulfolobus solfataricus P2, is regulated by acetylation of lysine 16. Here we identify an acetyltransferase that specifically acetylates Alba on this residue. The effect of acetylation is to lower the affinity of Alba for DNA. Remarkably, the acetyltransferase is conserved not only in archaea but also in bacteria where it appears to play a role in metabolic regulation. Therefore, our data suggest that S. solfataricus has co-opted this bacterial regulatory system to generate a rudimentary form of chromatin regulation. |
| doi_str_mv | 10.1074/jbc.M501280200 |
| format | Article |
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Here we identify an acetyltransferase that specifically acetylates Alba on this residue. The effect of acetylation is to lower the affinity of Alba for DNA. Remarkably, the acetyltransferase is conserved not only in archaea but also in bacteria where it appears to play a role in metabolic regulation. 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Therefore, our data suggest that S. solfataricus has co-opted this bacterial regulatory system to generate a rudimentary form of chromatin regulation.</description><subject>Acetyltransferases - chemistry</subject><subject>Acetyltransferases - metabolism</subject><subject>Acetyltransferases - physiology</subject><subject>Amino Acid Motifs</subject><subject>Amino Acid Sequence</subject><subject>Archaea</subject><subject>Archaeal Proteins - chemistry</subject><subject>Archaeal Proteins - metabolism</subject><subject>Chromatin - chemistry</subject><subject>Chromatin - metabolism</subject><subject>Chromatin Immunoprecipitation</subject><subject>Cloning, Molecular</subject><subject>DNA - chemistry</subject><subject>DNA - metabolism</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Gene Expression Regulation, Archaeal</subject><subject>Lysine - chemistry</subject><subject>Mass Spectrometry</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Sirtuins - chemistry</subject><subject>Sirtuins - metabolism</subject><subject>Sulfolobus solfataricus</subject><subject>Sulfolobus solfataricus - metabolism</subject><subject>Temperature</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9rFDEUx4NY7LZ69ShzEE87a15mskmOy2Kr0GKpCt7Cm8ybTsr8qElW6X_fyCz0JAZCDvl8v-_xYewt8A1wVX-8b9zmWnIQmgvOX7AVcF2VlYSfL9mKcwGlEVKfsrMY73k-tYFX7BSkFjUIsWK333wQBU5tkXoqdo7S45ACTrGjgJHWxQ2mdXFLd4cBEy1QcD0SDsW-D_OIyU_FTZgT-Wld7IYGX7OTDodIb47vOftx8en7_nN59fXyy353Vbpaq1Q6CVVHokGEuhIGuew0yrqptqCkwjZ_aWi0UbzW3EC-ynApeWUUOSOq6px9WHofwvzrQDHZ0UdHw4ATzYdot0ors5X6v2CeV2dtkMHNArowxxiosw_BjxgeLXD7V7fNuu2z7hx4d2w-NCO1z_jRbwbeL0Dv7_o_PpBt_Ox6Gm1usEJYAQumF4yyr9-ego3O0-SozRGXbDv7f63wBFrulq8</recordid><startdate>20050603</startdate><enddate>20050603</enddate><creator>Marsh, Victoria L.</creator><creator>Peak-Chew, Sew Yeu</creator><creator>Bell, Stephen D.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20050603</creationdate><title>Sir2 and the Acetyltransferase, Pat, Regulate the Archaeal Chromatin Protein, Alba</title><author>Marsh, Victoria L. ; 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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Acetyltransferases - chemistry Acetyltransferases - metabolism Acetyltransferases - physiology Amino Acid Motifs Amino Acid Sequence Archaea Archaeal Proteins - chemistry Archaeal Proteins - metabolism Chromatin - chemistry Chromatin - metabolism Chromatin Immunoprecipitation Cloning, Molecular DNA - chemistry DNA - metabolism DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Gene Expression Regulation Gene Expression Regulation, Archaeal Lysine - chemistry Mass Spectrometry Molecular Sequence Data Open Reading Frames Sirtuins - chemistry Sirtuins - metabolism Sulfolobus solfataricus Sulfolobus solfataricus - metabolism Temperature |
| title | Sir2 and the Acetyltransferase, Pat, Regulate the Archaeal Chromatin Protein, Alba |
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