Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin
OBJECTIVE—We recently isolated vasohibin, a novel vascular endothelial growth factor (VEGF)-inducible endothelium-derived angiogenesis inhibitor. Our aim is to find DNA sequences homologous to vasohibin and determine their expression profile. METHODS AND RESULTS—By the search of DNA sequences in the...
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| Veröffentlicht in: | Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2006-05, Vol.26 (5), p.1051-1057 |
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| creator | Shibuya, Takumi Watanabe, Kazuhide Yamashita, Hiroshi Shimizu, Kazue Miyashita, Hiroki Abe, Mayumi Moriya, Takuya Ohta, Hideki Sonoda, Hikaru Shimosegawa, Tooru Tabayashi, Koichi Sato, Yasufumi |
| description | OBJECTIVE—We recently isolated vasohibin, a novel vascular endothelial growth factor (VEGF)-inducible endothelium-derived angiogenesis inhibitor. Our aim is to find DNA sequences homologous to vasohibin and determine their expression profile.
METHODS AND RESULTS—By the search of DNA sequences in the database, we found one homologous gene and designated it vasohibin-2. Overall amino acid sequence homology between the prototype vasohibin (vasohibin-1) and vasohibin-2 was >50%. Vasohibin-2 exhibited antiangiogenic activity. Vasohibin-2 expression in cultured endothelial cells was low and not inducible by the stimulation that induced vasohibin-1. However, the immunohistochemical analysis revealed that vasohibin-1 and -2 were diffusely expressed in endothelial cells in embryonic organs during mid-gestation. After that time point, vasohibin-1 and -2 became faint, but persisted to a certain extent in arterial endothelial cells from late gestation to neonate. Expression of vasohibin-1 and -2 could be augmented in vivo by local transfection with the VEGF gene in the embryonic brain or by cutaneous wounding in adult mice.
CONCLUSION—These results suggest that vasohibin-2, in combination with vasohibin-1, forms a novel family of angiogenesis inhibitors. |
| doi_str_mv | 10.1161/01.ATV.0000216747.66660.26 |
| format | Article |
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METHODS AND RESULTS—By the search of DNA sequences in the database, we found one homologous gene and designated it vasohibin-2. Overall amino acid sequence homology between the prototype vasohibin (vasohibin-1) and vasohibin-2 was >50%. Vasohibin-2 exhibited antiangiogenic activity. Vasohibin-2 expression in cultured endothelial cells was low and not inducible by the stimulation that induced vasohibin-1. However, the immunohistochemical analysis revealed that vasohibin-1 and -2 were diffusely expressed in endothelial cells in embryonic organs during mid-gestation. After that time point, vasohibin-1 and -2 became faint, but persisted to a certain extent in arterial endothelial cells from late gestation to neonate. Expression of vasohibin-1 and -2 could be augmented in vivo by local transfection with the VEGF gene in the embryonic brain or by cutaneous wounding in adult mice.
CONCLUSION—These results suggest that vasohibin-2, in combination with vasohibin-1, forms a novel family of angiogenesis inhibitors.</description><identifier>ISSN: 1079-5642</identifier><identifier>EISSN: 1524-4636</identifier><identifier>DOI: 10.1161/01.ATV.0000216747.66660.26</identifier><identifier>PMID: 16528006</identifier><identifier>CODEN: ATVBFA</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Angiogenesis Inhibitors - analysis ; Angiogenesis Inhibitors - genetics ; Angiogenesis Inhibitors - pharmacology ; Animals ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Cardiovascular system ; Cells, Cultured ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Endothelial Cells - chemistry ; Humans ; Immunohistochemistry ; Investigative techniques of hemodynamics ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Mice ; Neovascularization, Physiologic - drug effects ; Organ Culture Techniques ; Pharmacology. Drug treatments ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Vasodilator agents. Cerebral vasodilators</subject><ispartof>Arteriosclerosis, thrombosis, and vascular biology, 2006-05, Vol.26 (5), p.1051-1057</ispartof><rights>2006 American Heart Association, Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5447-e425ba4b9c6cb7256427caf7062a8ea4d25f1f5d08c66632f5a242b11821edf03</citedby><cites>FETCH-LOGICAL-c5447-e425ba4b9c6cb7256427caf7062a8ea4d25f1f5d08c66632f5a242b11821edf03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17713208$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16528006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shibuya, Takumi</creatorcontrib><creatorcontrib>Watanabe, Kazuhide</creatorcontrib><creatorcontrib>Yamashita, Hiroshi</creatorcontrib><creatorcontrib>Shimizu, Kazue</creatorcontrib><creatorcontrib>Miyashita, Hiroki</creatorcontrib><creatorcontrib>Abe, Mayumi</creatorcontrib><creatorcontrib>Moriya, Takuya</creatorcontrib><creatorcontrib>Ohta, Hideki</creatorcontrib><creatorcontrib>Sonoda, Hikaru</creatorcontrib><creatorcontrib>Shimosegawa, Tooru</creatorcontrib><creatorcontrib>Tabayashi, Koichi</creatorcontrib><creatorcontrib>Sato, Yasufumi</creatorcontrib><title>Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin</title><title>Arteriosclerosis, thrombosis, and vascular biology</title><addtitle>Arterioscler Thromb Vasc Biol</addtitle><description>OBJECTIVE—We recently isolated vasohibin, a novel vascular endothelial growth factor (VEGF)-inducible endothelium-derived angiogenesis inhibitor. Our aim is to find DNA sequences homologous to vasohibin and determine their expression profile.
METHODS AND RESULTS—By the search of DNA sequences in the database, we found one homologous gene and designated it vasohibin-2. Overall amino acid sequence homology between the prototype vasohibin (vasohibin-1) and vasohibin-2 was >50%. Vasohibin-2 exhibited antiangiogenic activity. Vasohibin-2 expression in cultured endothelial cells was low and not inducible by the stimulation that induced vasohibin-1. However, the immunohistochemical analysis revealed that vasohibin-1 and -2 were diffusely expressed in endothelial cells in embryonic organs during mid-gestation. After that time point, vasohibin-1 and -2 became faint, but persisted to a certain extent in arterial endothelial cells from late gestation to neonate. Expression of vasohibin-1 and -2 could be augmented in vivo by local transfection with the VEGF gene in the embryonic brain or by cutaneous wounding in adult mice.
CONCLUSION—These results suggest that vasohibin-2, in combination with vasohibin-1, forms a novel family of angiogenesis inhibitors.</description><subject>Angiogenesis Inhibitors - analysis</subject><subject>Angiogenesis Inhibitors - genetics</subject><subject>Angiogenesis Inhibitors - pharmacology</subject><subject>Animals</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology. Vascular system</subject><subject>Cardiovascular system</subject><subject>Cells, Cultured</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</subject><subject>Endothelial Cells - chemistry</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Investigative techniques of hemodynamics</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Organ Culture Techniques</subject><subject>Pharmacology. Drug treatments</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Vasodilator agents. Cerebral vasodilators</subject><issn>1079-5642</issn><issn>1524-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1v3CAQhq2qVfPR_oUKVWpvuDDmw-lttd0kK0XqJc0VYYzXtBhSsBu11_7xsNmVdiTEAM-8I-atqo-U1JQK-oXQenX_UJMSQIVkshYlSA3iVXVOOTDMRCNel5zIK8wFg7PqIuefhWcA5G11RgWHlhBxXv3f5uj17GJAOvRoPeqkzWyT-3e4jAN60DmOrnMBA9IZaXQbp-jjbrEvr5uba7wN_WJc5y3ahD7Oo_VumfC3IvPH9mgVdi7ubLDZZbQNe605ppPuu-rNoH2274_7ZfXjenO_vsV332-269UdNpwxiS0D3mnWXRlhOgn7f0mjB0kE6NZq1gMf6MB70poyjgYGroFBR2kL1PYDaS6rzwfdxxR_LzbPanLZWO91sHHJSshWSiFlAb8eQJNizskO6jG5Sae_ihK1t0ARqooF6mSBerFAgSjFH45dlm6y_an0OPMCfDoCOhvth6SDcfnESUkbIG3h2IF7ir44kn_55ckmNVrt53HfmjWCcAxFk_ByxGVR2TwDS8Cf-g</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Shibuya, Takumi</creator><creator>Watanabe, Kazuhide</creator><creator>Yamashita, Hiroshi</creator><creator>Shimizu, Kazue</creator><creator>Miyashita, Hiroki</creator><creator>Abe, Mayumi</creator><creator>Moriya, Takuya</creator><creator>Ohta, Hideki</creator><creator>Sonoda, Hikaru</creator><creator>Shimosegawa, Tooru</creator><creator>Tabayashi, Koichi</creator><creator>Sato, Yasufumi</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200605</creationdate><title>Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin</title><author>Shibuya, Takumi ; Watanabe, Kazuhide ; Yamashita, Hiroshi ; Shimizu, Kazue ; Miyashita, Hiroki ; Abe, Mayumi ; Moriya, Takuya ; Ohta, Hideki ; Sonoda, Hikaru ; Shimosegawa, Tooru ; Tabayashi, Koichi ; Sato, Yasufumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5447-e425ba4b9c6cb7256427caf7062a8ea4d25f1f5d08c66632f5a242b11821edf03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Angiogenesis Inhibitors - analysis</topic><topic>Angiogenesis Inhibitors - genetics</topic><topic>Angiogenesis Inhibitors - pharmacology</topic><topic>Animals</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology. Vascular system</topic><topic>Cardiovascular system</topic><topic>Cells, Cultured</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Endothelial Cells - chemistry</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Investigative techniques of hemodynamics</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Organ Culture Techniques</topic><topic>Pharmacology. Drug treatments</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Vasodilator agents. Cerebral vasodilators</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shibuya, Takumi</creatorcontrib><creatorcontrib>Watanabe, Kazuhide</creatorcontrib><creatorcontrib>Yamashita, Hiroshi</creatorcontrib><creatorcontrib>Shimizu, Kazue</creatorcontrib><creatorcontrib>Miyashita, Hiroki</creatorcontrib><creatorcontrib>Abe, Mayumi</creatorcontrib><creatorcontrib>Moriya, Takuya</creatorcontrib><creatorcontrib>Ohta, Hideki</creatorcontrib><creatorcontrib>Sonoda, Hikaru</creatorcontrib><creatorcontrib>Shimosegawa, Tooru</creatorcontrib><creatorcontrib>Tabayashi, Koichi</creatorcontrib><creatorcontrib>Sato, Yasufumi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shibuya, Takumi</au><au>Watanabe, Kazuhide</au><au>Yamashita, Hiroshi</au><au>Shimizu, Kazue</au><au>Miyashita, Hiroki</au><au>Abe, Mayumi</au><au>Moriya, Takuya</au><au>Ohta, Hideki</au><au>Sonoda, Hikaru</au><au>Shimosegawa, Tooru</au><au>Tabayashi, Koichi</au><au>Sato, Yasufumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin</atitle><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle><addtitle>Arterioscler Thromb Vasc Biol</addtitle><date>2006-05</date><risdate>2006</risdate><volume>26</volume><issue>5</issue><spage>1051</spage><epage>1057</epage><pages>1051-1057</pages><issn>1079-5642</issn><eissn>1524-4636</eissn><coden>ATVBFA</coden><abstract>OBJECTIVE—We recently isolated vasohibin, a novel vascular endothelial growth factor (VEGF)-inducible endothelium-derived angiogenesis inhibitor. Our aim is to find DNA sequences homologous to vasohibin and determine their expression profile.
METHODS AND RESULTS—By the search of DNA sequences in the database, we found one homologous gene and designated it vasohibin-2. Overall amino acid sequence homology between the prototype vasohibin (vasohibin-1) and vasohibin-2 was >50%. Vasohibin-2 exhibited antiangiogenic activity. Vasohibin-2 expression in cultured endothelial cells was low and not inducible by the stimulation that induced vasohibin-1. However, the immunohistochemical analysis revealed that vasohibin-1 and -2 were diffusely expressed in endothelial cells in embryonic organs during mid-gestation. After that time point, vasohibin-1 and -2 became faint, but persisted to a certain extent in arterial endothelial cells from late gestation to neonate. Expression of vasohibin-1 and -2 could be augmented in vivo by local transfection with the VEGF gene in the embryonic brain or by cutaneous wounding in adult mice.
CONCLUSION—These results suggest that vasohibin-2, in combination with vasohibin-1, forms a novel family of angiogenesis inhibitors.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>16528006</pmid><doi>10.1161/01.ATV.0000216747.66660.26</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Angiogenesis Inhibitors - analysis Angiogenesis Inhibitors - genetics Angiogenesis Inhibitors - pharmacology Animals Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Cardiovascular system Cells, Cultured Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Endothelial Cells - chemistry Humans Immunohistochemistry Investigative techniques of hemodynamics Investigative techniques, diagnostic techniques (general aspects) Medical sciences Mice Neovascularization, Physiologic - drug effects Organ Culture Techniques Pharmacology. Drug treatments Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Vasodilator agents. Cerebral vasodilators |
| title | Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin |
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