Heterogeneous resistance to vancomycin in Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening
The population analysis profile (PAP) method as well as analysis of autolytic activity and cellular ultrastructure by transmission electron microscopy (TEM) were used to characterise Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri clinical strains with reduced susc...
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| Veröffentlicht in: | International journal of antimicrobial agents 2006-04, Vol.27 (4), p.307-315 |
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| creator | Nunes, Ana Paula Ferreira Teixeira, Lúcia Martins Iorio, Natália Lopes Pontes Bastos, Carla Callegário Reis Fonseca, Leila de Sousa Souto-Padrón, Thaís dos Santos, Kátia Regina Netto |
| description | The population analysis profile (PAP) method as well as analysis of autolytic activity and cellular ultrastructure by transmission electron microscopy (TEM) were used to characterise
Staphylococcus epidermidis,
Staphylococcus haemolyticus and
Staphylococcus warneri clinical strains with reduced susceptibility to glycopeptides. All strains showed heterogeneous profiles to vancomycin and teicoplanin by the PAP method. Subpopulations that grew in the presence of high concentrations of each drug were selected from the PAP as derivative strains. Their glycopeptide minimal inhibitory concentrations (MICs) were determined and subsequently all parental and derivative strains were grown in one-half of the MIC of vancomycin or teicoplanin. An increase in cell wall thickness of all derivative strains was seen by TEM, with statistically significant values (
P
<
0.01) compared with their respective parental strains. In general, variable rates of autolysis among the strains were observed. Cell wall thickness is an important factor involved in glycopeptide resistance and, in association with PAP results, confirmed the Brazilian coagulase-negative staphylococci clinical isolates as being heteroresistant to glycopeptides. Detection of these heteroresistant organisms is important in order to achieve more judicious use of vancomycin and teicoplanin in hospitals. |
| doi_str_mv | 10.1016/j.ijantimicag.2005.11.013 |
| format | Article |
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Staphylococcus epidermidis,
Staphylococcus haemolyticus and
Staphylococcus warneri clinical strains with reduced susceptibility to glycopeptides. All strains showed heterogeneous profiles to vancomycin and teicoplanin by the PAP method. Subpopulations that grew in the presence of high concentrations of each drug were selected from the PAP as derivative strains. Their glycopeptide minimal inhibitory concentrations (MICs) were determined and subsequently all parental and derivative strains were grown in one-half of the MIC of vancomycin or teicoplanin. An increase in cell wall thickness of all derivative strains was seen by TEM, with statistically significant values (
P
<
0.01) compared with their respective parental strains. In general, variable rates of autolysis among the strains were observed. Cell wall thickness is an important factor involved in glycopeptide resistance and, in association with PAP results, confirmed the Brazilian coagulase-negative staphylococci clinical isolates as being heteroresistant to glycopeptides. Detection of these heteroresistant organisms is important in order to achieve more judicious use of vancomycin and teicoplanin in hospitals.</description><identifier>ISSN: 0924-8579</identifier><identifier>EISSN: 1872-7913</identifier><identifier>DOI: 10.1016/j.ijantimicag.2005.11.013</identifier><identifier>PMID: 16542825</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Bacteriolysis ; Biological and medical sciences ; Brazil ; Cell Wall - ultrastructure ; Colony Count, Microbial ; Heteroresistance ; Humans ; Medical sciences ; Microbial Sensitivity Tests ; Microscopy, Electron, Transmission ; Pharmacology. Drug treatments ; Population analysis profile ; Staphylococcal Infections - microbiology ; Staphylococci ; Staphylococcus - drug effects ; Staphylococcus - isolation & purification ; Staphylococcus - ultrastructure ; Staphylococcus epidermidis ; Staphylococcus epidermidis - drug effects ; Staphylococcus epidermidis - isolation & purification ; Staphylococcus epidermidis - ultrastructure ; Staphylococcus haemolyticus ; Staphylococcus haemolyticus - drug effects ; Staphylococcus haemolyticus - isolation & purification ; Staphylococcus warneri ; Teicoplanin ; Teicoplanin - pharmacology ; Transmission electron microscopy ; Vancomycin ; Vancomycin Resistance</subject><ispartof>International journal of antimicrobial agents, 2006-04, Vol.27 (4), p.307-315</ispartof><rights>2006 Elsevier B.V. and the International Society of Chemotherapy</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-8d6facc19a79edb698cfb3d28de7940e227b963ca6295480f89d833f4c94d4823</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0924857906000252$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17685250$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16542825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nunes, Ana Paula Ferreira</creatorcontrib><creatorcontrib>Teixeira, Lúcia Martins</creatorcontrib><creatorcontrib>Iorio, Natália Lopes Pontes</creatorcontrib><creatorcontrib>Bastos, Carla Callegário Reis</creatorcontrib><creatorcontrib>Fonseca, Leila de Sousa</creatorcontrib><creatorcontrib>Souto-Padrón, Thaís</creatorcontrib><creatorcontrib>dos Santos, Kátia Regina Netto</creatorcontrib><title>Heterogeneous resistance to vancomycin in Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening</title><title>International journal of antimicrobial agents</title><addtitle>Int J Antimicrob Agents</addtitle><description>The population analysis profile (PAP) method as well as analysis of autolytic activity and cellular ultrastructure by transmission electron microscopy (TEM) were used to characterise
Staphylococcus epidermidis,
Staphylococcus haemolyticus and
Staphylococcus warneri clinical strains with reduced susceptibility to glycopeptides. All strains showed heterogeneous profiles to vancomycin and teicoplanin by the PAP method. Subpopulations that grew in the presence of high concentrations of each drug were selected from the PAP as derivative strains. Their glycopeptide minimal inhibitory concentrations (MICs) were determined and subsequently all parental and derivative strains were grown in one-half of the MIC of vancomycin or teicoplanin. An increase in cell wall thickness of all derivative strains was seen by TEM, with statistically significant values (
P
<
0.01) compared with their respective parental strains. In general, variable rates of autolysis among the strains were observed. Cell wall thickness is an important factor involved in glycopeptide resistance and, in association with PAP results, confirmed the Brazilian coagulase-negative staphylococci clinical isolates as being heteroresistant to glycopeptides. Detection of these heteroresistant organisms is important in order to achieve more judicious use of vancomycin and teicoplanin in hospitals.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Bacteriolysis</subject><subject>Biological and medical sciences</subject><subject>Brazil</subject><subject>Cell Wall - ultrastructure</subject><subject>Colony Count, Microbial</subject><subject>Heteroresistance</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Microscopy, Electron, Transmission</subject><subject>Pharmacology. Drug treatments</subject><subject>Population analysis profile</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococci</subject><subject>Staphylococcus - drug effects</subject><subject>Staphylococcus - isolation & purification</subject><subject>Staphylococcus - ultrastructure</subject><subject>Staphylococcus epidermidis</subject><subject>Staphylococcus epidermidis - drug effects</subject><subject>Staphylococcus epidermidis - isolation & purification</subject><subject>Staphylococcus epidermidis - ultrastructure</subject><subject>Staphylococcus haemolyticus</subject><subject>Staphylococcus haemolyticus - drug effects</subject><subject>Staphylococcus haemolyticus - isolation & purification</subject><subject>Staphylococcus warneri</subject><subject>Teicoplanin</subject><subject>Teicoplanin - pharmacology</subject><subject>Transmission electron microscopy</subject><subject>Vancomycin</subject><subject>Vancomycin Resistance</subject><issn>0924-8579</issn><issn>1872-7913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks2OFCEQxztG446rr2DwoKedEegPwJuZuK7JJh7UM2GgeqbGbmiBWdNv66PIOJOs2YsmBIrUrz6g_lX1itEVo6x7u1_h3viMI1qzXXFK2xVjK8rqR9WCScGXQrH6cbWgijdL2Qp1UT1LaU8pa-umfVpdsK5tuOTtovp1Axli2IKHcEgkQsKUjbdAciB3xQjjbNGTsr5kM-3mIdhgbUFhQgdxRIfp6qFvZ2AMw5zxeDHePfT_NNFDRGIH9OUNA0k5GvTpHbE7E40tLWEyGYMnoSfbYbZhgimXgiQdkj2aGxwwz2SKoccBTlUsDEPJXba8Q_sdPPrt8-pJb4YEL87nZfXt-sPX9c3y9vPHT-v3t0vb1F1eStf1xlqmjFDgNp2Stt_UjksHQjUUOBcb1dXWdFy1jaS9VE7Wdd9Y1bhG8vqyenPKWzr6cYCU9Yjp2JD587O6E1JQpcQ_QSaY6FrJCqhOoI0hpQi9niKOJs6aUX3Ugd7rv3SgjzrQjOmigxL78lzksBnB3UeeB1-A12fApDKAPpZRY7rnRCdb3tLCrU8clL-7Q4g6WYSiD4cRbNYu4H-08xvLEOB7</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Nunes, Ana Paula Ferreira</creator><creator>Teixeira, Lúcia Martins</creator><creator>Iorio, Natália Lopes Pontes</creator><creator>Bastos, Carla Callegário Reis</creator><creator>Fonseca, Leila de Sousa</creator><creator>Souto-Padrón, Thaís</creator><creator>dos Santos, Kátia Regina Netto</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Heterogeneous resistance to vancomycin in Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening</title><author>Nunes, Ana Paula Ferreira ; Teixeira, Lúcia Martins ; Iorio, Natália Lopes Pontes ; Bastos, Carla Callegário Reis ; Fonseca, Leila de Sousa ; Souto-Padrón, Thaís ; dos Santos, Kátia Regina Netto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-8d6facc19a79edb698cfb3d28de7940e227b963ca6295480f89d833f4c94d4823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Bacteriolysis</topic><topic>Biological and medical sciences</topic><topic>Brazil</topic><topic>Cell Wall - ultrastructure</topic><topic>Colony Count, Microbial</topic><topic>Heteroresistance</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Microscopy, Electron, Transmission</topic><topic>Pharmacology. Drug treatments</topic><topic>Population analysis profile</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococci</topic><topic>Staphylococcus - drug effects</topic><topic>Staphylococcus - isolation & purification</topic><topic>Staphylococcus - ultrastructure</topic><topic>Staphylococcus epidermidis</topic><topic>Staphylococcus epidermidis - drug effects</topic><topic>Staphylococcus epidermidis - isolation & purification</topic><topic>Staphylococcus epidermidis - ultrastructure</topic><topic>Staphylococcus haemolyticus</topic><topic>Staphylococcus haemolyticus - drug effects</topic><topic>Staphylococcus haemolyticus - isolation & purification</topic><topic>Staphylococcus warneri</topic><topic>Teicoplanin</topic><topic>Teicoplanin - pharmacology</topic><topic>Transmission electron microscopy</topic><topic>Vancomycin</topic><topic>Vancomycin Resistance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nunes, Ana Paula Ferreira</creatorcontrib><creatorcontrib>Teixeira, Lúcia Martins</creatorcontrib><creatorcontrib>Iorio, Natália Lopes Pontes</creatorcontrib><creatorcontrib>Bastos, Carla Callegário Reis</creatorcontrib><creatorcontrib>Fonseca, Leila de Sousa</creatorcontrib><creatorcontrib>Souto-Padrón, Thaís</creatorcontrib><creatorcontrib>dos Santos, Kátia Regina Netto</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of antimicrobial agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nunes, Ana Paula Ferreira</au><au>Teixeira, Lúcia Martins</au><au>Iorio, Natália Lopes Pontes</au><au>Bastos, Carla Callegário Reis</au><au>Fonseca, Leila de Sousa</au><au>Souto-Padrón, Thaís</au><au>dos Santos, Kátia Regina Netto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterogeneous resistance to vancomycin in Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening</atitle><jtitle>International journal of antimicrobial agents</jtitle><addtitle>Int J Antimicrob Agents</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>27</volume><issue>4</issue><spage>307</spage><epage>315</epage><pages>307-315</pages><issn>0924-8579</issn><eissn>1872-7913</eissn><abstract>The population analysis profile (PAP) method as well as analysis of autolytic activity and cellular ultrastructure by transmission electron microscopy (TEM) were used to characterise
Staphylococcus epidermidis,
Staphylococcus haemolyticus and
Staphylococcus warneri clinical strains with reduced susceptibility to glycopeptides. All strains showed heterogeneous profiles to vancomycin and teicoplanin by the PAP method. Subpopulations that grew in the presence of high concentrations of each drug were selected from the PAP as derivative strains. Their glycopeptide minimal inhibitory concentrations (MICs) were determined and subsequently all parental and derivative strains were grown in one-half of the MIC of vancomycin or teicoplanin. An increase in cell wall thickness of all derivative strains was seen by TEM, with statistically significant values (
P
<
0.01) compared with their respective parental strains. In general, variable rates of autolysis among the strains were observed. Cell wall thickness is an important factor involved in glycopeptide resistance and, in association with PAP results, confirmed the Brazilian coagulase-negative staphylococci clinical isolates as being heteroresistant to glycopeptides. Detection of these heteroresistant organisms is important in order to achieve more judicious use of vancomycin and teicoplanin in hospitals.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>16542825</pmid><doi>10.1016/j.ijantimicag.2005.11.013</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0924-8579 |
| ispartof | International journal of antimicrobial agents, 2006-04, Vol.27 (4), p.307-315 |
| issn | 0924-8579 1872-7913 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anti-Bacterial Agents - pharmacology Antibiotics. Antiinfectious agents. Antiparasitic agents Bacteriolysis Biological and medical sciences Brazil Cell Wall - ultrastructure Colony Count, Microbial Heteroresistance Humans Medical sciences Microbial Sensitivity Tests Microscopy, Electron, Transmission Pharmacology. Drug treatments Population analysis profile Staphylococcal Infections - microbiology Staphylococci Staphylococcus - drug effects Staphylococcus - isolation & purification Staphylococcus - ultrastructure Staphylococcus epidermidis Staphylococcus epidermidis - drug effects Staphylococcus epidermidis - isolation & purification Staphylococcus epidermidis - ultrastructure Staphylococcus haemolyticus Staphylococcus haemolyticus - drug effects Staphylococcus haemolyticus - isolation & purification Staphylococcus warneri Teicoplanin Teicoplanin - pharmacology Transmission electron microscopy Vancomycin Vancomycin Resistance |
| title | Heterogeneous resistance to vancomycin in Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening |
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