Transcriptome comparison of murine wild-type and synaptophysin-deficient retina reveals complete identity

Loss of synaptophysin, one of the major synaptic vesicle membrane proteins, is surprisingly well tolerated in knockout mice. To test whether compensatory gene transcription accounts for the apparent lack of functional deficiencies, comparative transcriptome analyses were carried out. The retina was...

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Veröffentlicht in:Brain research 2006-04, Vol.1081 (1), p.53-58
Hauptverfasser: Bai, Lin, Spiwoks-Becker, Isabella, Leube, Rudolf E.
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creator Bai, Lin
Spiwoks-Becker, Isabella
Leube, Rudolf E.
description Loss of synaptophysin, one of the major synaptic vesicle membrane proteins, is surprisingly well tolerated in knockout mice. To test whether compensatory gene transcription accounts for the apparent lack of functional deficiencies, comparative transcriptome analyses were carried out. The retina was selected as the most suitable tissue since morphological alterations were observed in mutant photoreceptors, most notably a reduction of synaptic vesicles and concomitant increase in clathrin-coated vesicles. Labeled cRNA was prepared in triplicate from retinae of age- and sex-matched wild-type and mutant litter mates and hybridized to high-density microarray chips. Only three differentially expressed RNAs were identified in this way, one of which was synaptophysin. Further validation by quantitative RT-PCR could only corroborate the results for the latter. We therefore conclude that, despite the distinct morphological phenotype, no significant changes in gene expression are detectable in synaptophysin-deficient animals and that therefore compensatory mechanisms are either pre-existent and/or act at the posttranscriptional level.
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To test whether compensatory gene transcription accounts for the apparent lack of functional deficiencies, comparative transcriptome analyses were carried out. The retina was selected as the most suitable tissue since morphological alterations were observed in mutant photoreceptors, most notably a reduction of synaptic vesicles and concomitant increase in clathrin-coated vesicles. Labeled cRNA was prepared in triplicate from retinae of age- and sex-matched wild-type and mutant litter mates and hybridized to high-density microarray chips. Only three differentially expressed RNAs were identified in this way, one of which was synaptophysin. Further validation by quantitative RT-PCR could only corroborate the results for the latter. 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subjects Animals
Biological and medical sciences
Clathrin - metabolism
Eye and associated structures. Visual pathways and centers. Vision
Eye Proteins - genetics
Eye Proteins - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Glucan 1,3-beta-Glucosidase
High-density microarray
Knockout
Mice
Mice, Inbred C57BL
Mice, Knockout
Microarray Analysis - methods
Microscopy, Electron, Transmission - methods
Photoreceptor
Photoreceptor Cells - metabolism
Photoreceptor Cells - ultrastructure
Retina - metabolism
Retina - ultrastructure
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - biosynthesis
Synapse
Synaptic vesicle
Synaptic Vesicles - metabolism
Synaptic Vesicles - ultrastructure
Synaptophysin - deficiency
Vertebrates: nervous system and sense organs
title Transcriptome comparison of murine wild-type and synaptophysin-deficient retina reveals complete identity
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