Embryonic Synthesis of the Inner Limiting Membrane and Vitreous Body
The inner limiting membrane (ILM) and the vitreous body (VB) are major parts of the extracellular matrix of the eye. The present study was undertaken to investigate the synthesis and turnover of the ILM and VB in chick and human embryonic and postembryonic eye development. The abundance of ILM and V...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2005-06, Vol.46 (6), p.2202-2209 |
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| container_title | Investigative ophthalmology & visual science |
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| creator | Halfter, Willi Dong, Sucai Schurer, Barbara Ring, Colleen Cole, Gregory J Eller, Andrew |
| description | The inner limiting membrane (ILM) and the vitreous body (VB) are major parts of the extracellular matrix of the eye. The present study was undertaken to investigate the synthesis and turnover of the ILM and VB in chick and human embryonic and postembryonic eye development.
The abundance of ILM and VB proteins was determined by Western blot analysis using samples from chick and human VB of different ages. The mRNA expression of the ILM proteins in lens was determined by in situ hybridization and RT-PCR.
Based on the abundance of mRNA expression, the prominent sources of ILM and VB proteins in chick eyes are the lens and ciliary body. In chick, ILM and VB matrix proteins were most abundant in embryonic VB, and their concentration declined precipitously after hatching. Most ILM and VB proteins were no longer detectable in the adult VB. In humans, a similar developmentally regulated expression of ILM and VB proteins in VB was detected: The highest concentrations of ILM and VB proteins were detected in fetal VB, the lowest in the adult VB. The decline in ILM and VB protein synthesis occurred within the first 2 years of life.
The abundance of ILM and VB proteins in the embryonic VB, their sharp decline at postembryonic stages, and their very low abundance in the adult VB show that ILM and VB are assembled during embryogenesis and are maintained throughout life with minimum turnover. |
| doi_str_mv | 10.1167/iovs.04-1419 |
| format | Article |
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The abundance of ILM and VB proteins was determined by Western blot analysis using samples from chick and human VB of different ages. The mRNA expression of the ILM proteins in lens was determined by in situ hybridization and RT-PCR.
Based on the abundance of mRNA expression, the prominent sources of ILM and VB proteins in chick eyes are the lens and ciliary body. In chick, ILM and VB matrix proteins were most abundant in embryonic VB, and their concentration declined precipitously after hatching. Most ILM and VB proteins were no longer detectable in the adult VB. In humans, a similar developmentally regulated expression of ILM and VB proteins in VB was detected: The highest concentrations of ILM and VB proteins were detected in fetal VB, the lowest in the adult VB. The decline in ILM and VB protein synthesis occurred within the first 2 years of life.
The abundance of ILM and VB proteins in the embryonic VB, their sharp decline at postembryonic stages, and their very low abundance in the adult VB show that ILM and VB are assembled during embryogenesis and are maintained throughout life with minimum turnover.</description><identifier>ISSN: 0146-0404</identifier><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.04-1419</identifier><identifier>PMID: 15914642</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Adult ; Animals ; Basement Membrane - embryology ; Basement Membrane - metabolism ; Biological and medical sciences ; Blotting, Western ; Chick Embryo ; Chickens ; Embryonic Development - physiology ; Extracellular Matrix Proteins - biosynthesis ; Extracellular Matrix Proteins - genetics ; Eye and associated structures. Visual pathways and centers. Vision ; Eye Proteins - biosynthesis ; Eye Proteins - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - physiology ; Humans ; In Situ Hybridization ; Infant ; Infant, Newborn ; Retina - embryology ; Retina - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Vertebrates: nervous system and sense organs ; Vitreous Body - embryology ; Vitreous Body - metabolism</subject><ispartof>Investigative ophthalmology & visual science, 2005-06, Vol.46 (6), p.2202-2209</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-b452d535a527e90176905608d18343f76824097f4bed1d42015ed0489134a34e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16816172$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15914642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Halfter, Willi</creatorcontrib><creatorcontrib>Dong, Sucai</creatorcontrib><creatorcontrib>Schurer, Barbara</creatorcontrib><creatorcontrib>Ring, Colleen</creatorcontrib><creatorcontrib>Cole, Gregory J</creatorcontrib><creatorcontrib>Eller, Andrew</creatorcontrib><title>Embryonic Synthesis of the Inner Limiting Membrane and Vitreous Body</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>The inner limiting membrane (ILM) and the vitreous body (VB) are major parts of the extracellular matrix of the eye. The present study was undertaken to investigate the synthesis and turnover of the ILM and VB in chick and human embryonic and postembryonic eye development.
The abundance of ILM and VB proteins was determined by Western blot analysis using samples from chick and human VB of different ages. The mRNA expression of the ILM proteins in lens was determined by in situ hybridization and RT-PCR.
Based on the abundance of mRNA expression, the prominent sources of ILM and VB proteins in chick eyes are the lens and ciliary body. In chick, ILM and VB matrix proteins were most abundant in embryonic VB, and their concentration declined precipitously after hatching. Most ILM and VB proteins were no longer detectable in the adult VB. In humans, a similar developmentally regulated expression of ILM and VB proteins in VB was detected: The highest concentrations of ILM and VB proteins were detected in fetal VB, the lowest in the adult VB. The decline in ILM and VB protein synthesis occurred within the first 2 years of life.
The abundance of ILM and VB proteins in the embryonic VB, their sharp decline at postembryonic stages, and their very low abundance in the adult VB show that ILM and VB are assembled during embryogenesis and are maintained throughout life with minimum turnover.</description><subject>Adult</subject><subject>Animals</subject><subject>Basement Membrane - embryology</subject><subject>Basement Membrane - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Embryonic Development - physiology</subject><subject>Extracellular Matrix Proteins - biosynthesis</subject><subject>Extracellular Matrix Proteins - genetics</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Eye Proteins - biosynthesis</subject><subject>Eye Proteins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Retina - embryology</subject><subject>Retina - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Vitreous Body - embryology</subject><subject>Vitreous Body - metabolism</subject><issn>0146-0404</issn><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtP6zAQhS0Egl5gxxp5A6sbmLHHTrLkdQGpiAWPreUmDjXKA-yUqv_-uqJSV3Ok-XR09DF2gnCBqPNLP_zEC6AMCcsdNkGlRKbyQu6yCSDpDAjogP2J8RNAIArYZweoyvQiMWG3d90srIbeV_xl1Y9zF33kQ8NT4o997wKf-s6Pvv_gTy6htnfc9jV_92NwwyLy66FeHbG9xrbRHW_uIXv7d_d685BNn-8fb66mWUWKxmxGStRKKqtE7krAXJegNBQ1FpJkk-tCEJR5QzNXY00CULkaqChRkpXk5CE7_-39CsP3wsXRdD5Wrm3TqrTF6LzQilAm8O8vWIUhxuAa8xV8Z8PKIJi1NbO2ZoDM2lrCTze9i1nn6i280ZSAsw1gY2XbJmmofNxyukCNudgOnPuP-dIHZ2Jn2zbVolkul6SNNkKAkP8BHvKATg</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Halfter, Willi</creator><creator>Dong, Sucai</creator><creator>Schurer, Barbara</creator><creator>Ring, Colleen</creator><creator>Cole, Gregory J</creator><creator>Eller, Andrew</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Embryonic Synthesis of the Inner Limiting Membrane and Vitreous Body</title><author>Halfter, Willi ; Dong, Sucai ; Schurer, Barbara ; Ring, Colleen ; Cole, Gregory J ; Eller, Andrew</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-b452d535a527e90176905608d18343f76824097f4bed1d42015ed0489134a34e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Basement Membrane - embryology</topic><topic>Basement Membrane - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Chick Embryo</topic><topic>Chickens</topic><topic>Embryonic Development - physiology</topic><topic>Extracellular Matrix Proteins - biosynthesis</topic><topic>Extracellular Matrix Proteins - genetics</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Eye Proteins - biosynthesis</topic><topic>Eye Proteins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Retina - embryology</topic><topic>Retina - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Vitreous Body - embryology</topic><topic>Vitreous Body - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Halfter, Willi</creatorcontrib><creatorcontrib>Dong, Sucai</creatorcontrib><creatorcontrib>Schurer, Barbara</creatorcontrib><creatorcontrib>Ring, Colleen</creatorcontrib><creatorcontrib>Cole, Gregory J</creatorcontrib><creatorcontrib>Eller, Andrew</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Halfter, Willi</au><au>Dong, Sucai</au><au>Schurer, Barbara</au><au>Ring, Colleen</au><au>Cole, Gregory J</au><au>Eller, Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Embryonic Synthesis of the Inner Limiting Membrane and Vitreous Body</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>46</volume><issue>6</issue><spage>2202</spage><epage>2209</epage><pages>2202-2209</pages><issn>0146-0404</issn><issn>1552-5783</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>The inner limiting membrane (ILM) and the vitreous body (VB) are major parts of the extracellular matrix of the eye. The present study was undertaken to investigate the synthesis and turnover of the ILM and VB in chick and human embryonic and postembryonic eye development.
The abundance of ILM and VB proteins was determined by Western blot analysis using samples from chick and human VB of different ages. The mRNA expression of the ILM proteins in lens was determined by in situ hybridization and RT-PCR.
Based on the abundance of mRNA expression, the prominent sources of ILM and VB proteins in chick eyes are the lens and ciliary body. In chick, ILM and VB matrix proteins were most abundant in embryonic VB, and their concentration declined precipitously after hatching. Most ILM and VB proteins were no longer detectable in the adult VB. In humans, a similar developmentally regulated expression of ILM and VB proteins in VB was detected: The highest concentrations of ILM and VB proteins were detected in fetal VB, the lowest in the adult VB. The decline in ILM and VB protein synthesis occurred within the first 2 years of life.
The abundance of ILM and VB proteins in the embryonic VB, their sharp decline at postembryonic stages, and their very low abundance in the adult VB show that ILM and VB are assembled during embryogenesis and are maintained throughout life with minimum turnover.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>15914642</pmid><doi>10.1167/iovs.04-1419</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Animals Basement Membrane - embryology Basement Membrane - metabolism Biological and medical sciences Blotting, Western Chick Embryo Chickens Embryonic Development - physiology Extracellular Matrix Proteins - biosynthesis Extracellular Matrix Proteins - genetics Eye and associated structures. Visual pathways and centers. Vision Eye Proteins - biosynthesis Eye Proteins - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - physiology Humans In Situ Hybridization Infant Infant, Newborn Retina - embryology Retina - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Vertebrates: nervous system and sense organs Vitreous Body - embryology Vitreous Body - metabolism |
| title | Embryonic Synthesis of the Inner Limiting Membrane and Vitreous Body |
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