Fluorescent Toolbox for Assessing Protein Location and Function
Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection i...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2006-04, Vol.312 (5771), p.217-224 |
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| creator | Giepmans, Ben N. G Adams, Stephen R Ellisman, Mark H Tsien, Roger Y |
| description | Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy. Small organic fluorescent dyes, nanocrystals ("quantum dots"), autofluorescent proteins, small genetic encoded tags that can be complexed with fluorochromes, and combinations of these probes are highlighted. |
| doi_str_mv | 10.1126/science.1124618 |
| format | Article |
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| ispartof | Science (American Association for the Advancement of Science), 2006-04, Vol.312 (5771), p.217-224 |
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| source | MEDLINE; Science Magazine; JSTOR Archive Collection A-Z Listing |
| subjects | Antibodies Biochemistry Cells Diffusion Dyes Enzymes - metabolism Fluorescence Fluorescence Resonance Energy Transfer Fluorescent Antibody Technique Fluorescent Dyes - chemistry Genetic Techniques Imaging Immunohistochemistry Luminescent Proteins - chemistry Luminescent Proteins - genetics Medical genetics Microscopy Microscopy, Electron Molecular Probe Techniques Protein Conformation Protein Transport Proteins Proteins - analysis Proteins - chemistry Proteins - metabolism Proteins - physiology Quantum Dots Receptors Reviews Wavelengths |
| title | Fluorescent Toolbox for Assessing Protein Location and Function |
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