Design and evaluation of an oligonucleotide-microarray for the detection of different species of the genus Kitasatospora

An oligonucleotide-microarray method was developed for the detection of Kitasatospora species in soil samples. The 16S–23S rDNA internal transcribed spacer (ITS) sequence of these antibiotics-producing actinomycetes was applied to design short oligonucleotide probes. Two different 26-mers were synth...

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Veröffentlicht in:	Journal of microbiological methods 2006-05, Vol.65 (2), p.226-236
Hauptverfasser:	Günther, Sebastian, Groth, Ingrid, Grabley, Susanne, Munder, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	16S–23S rDNA Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences DNA, Bacterial - analysis DNA, Bacterial - isolation & purification DNA, Ribosomal Spacer - analysis DNA, Ribosomal Spacer - genetics Fundamental and applied biological sciences. Psychology Genetics Internal transcribed spacer Kitasatospora Microbiology Oligonucleotide Array Sequence Analysis - methods Oligonucleotide microarray Oligonucleotide Probes - genetics Polymerase Chain Reaction RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics Soil Microbiology Soil samples Species Specificity Streptomyces Streptomycetaceae - classification Streptomycetaceae - growth & development Streptomycetaceae - isolation & purification
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