Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria

A method for determination of plasmid copy number (PCN) in bacteria by real-time quantitative polymerase chain reaction (QPCR) was developed as an alternative to current PCN assays. Conventional methods for PCN estimation are generally not of high throughput, laborious, have low reproducibility, req...

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Veröffentlicht in:	Journal of microbiological methods 2006-05, Vol.65 (2), p.258-267
Hauptverfasser:	Lee, Chai Lian, Ow, Dave Siak Wei, Oh, Steve Kah Weng
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Biological and medical sciences Culture Media DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Escherichia coli Escherichia coli - genetics Fermentation Fundamental and applied biological sciences. Psychology Gene Dosage - genetics Genetics Microbiology Plasmid copy number Plasmids - genetics Polymerase Chain Reaction - methods pUC origin of replication Real-time PCR Reproducibility of Results Sensitivity and Specificity SYBR Green
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