A new murine tumor model for studying HLA-A2-restricted anti-tumor immunity
HLA-A2/K b transgenic mice have been powerful tools for studying HLA-A2-restricted anti-tumor immunity. Two tumor lines were established from an aged HLA-A2/K b transgenic mouse that developed spontaneous tumors in the right limb and lung. Histopathologic analysis of the tumor was consistent with an...
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| Veröffentlicht in: | Cancer letters 2005-06, Vol.224 (1), p.153-166 |
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| creator | Kaplan, Barbara L.F. Moore, Tamson V. Schreiber, Karin Callender, Glenda G. Schreiber, Hans Nishimura, Michael I. |
| description | HLA-A2/K
b transgenic mice have been powerful tools for studying HLA-A2-restricted anti-tumor immunity. Two tumor lines were established from an aged HLA-A2/K
b transgenic mouse that developed spontaneous tumors in the right limb and lung. Histopathologic analysis of the tumor was consistent with an osteosarcoma that had metastasized to the lung. The cells from the primary tumor and the lung metastasis were adapted to culture and are designated Ag201P and Ag201M, respectively. Both Ag201P and Ag201M induced tumors in mice, indicating that the established cell lines are tumorigenic. Both tumor lines expressed HLA-A2/K
b as assessed by RT-PCR and immunofluorescence analysis. Furthermore, the HLA-A2/K
b molecules were functional on both tumor lines as demonstrated by their ability to present exogenously loaded HLA-A2-restricted peptides to human HLA-A2-restricted T cells. More importantly, endogenously expressed HLA-A2-restricted epitopes were processed and presented in the context of HLA-A2/K
b in Ag201P and Ag201M cells to human HLA-A2-restricted T cells. Thus, Ag201P and Ag201M are two new murine tumor lines that express functional HLA-A2/K
b, and represent potentially invaluable tools to study HLA-A2-restricted anti-tumor immunity in mice. |
| doi_str_mv | 10.1016/j.canlet.2004.11.035 |
| format | Article |
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b transgenic mice have been powerful tools for studying HLA-A2-restricted anti-tumor immunity. Two tumor lines were established from an aged HLA-A2/K
b transgenic mouse that developed spontaneous tumors in the right limb and lung. Histopathologic analysis of the tumor was consistent with an osteosarcoma that had metastasized to the lung. The cells from the primary tumor and the lung metastasis were adapted to culture and are designated Ag201P and Ag201M, respectively. Both Ag201P and Ag201M induced tumors in mice, indicating that the established cell lines are tumorigenic. Both tumor lines expressed HLA-A2/K
b as assessed by RT-PCR and immunofluorescence analysis. Furthermore, the HLA-A2/K
b molecules were functional on both tumor lines as demonstrated by their ability to present exogenously loaded HLA-A2-restricted peptides to human HLA-A2-restricted T cells. More importantly, endogenously expressed HLA-A2-restricted epitopes were processed and presented in the context of HLA-A2/K
b in Ag201P and Ag201M cells to human HLA-A2-restricted T cells. Thus, Ag201P and Ag201M are two new murine tumor lines that express functional HLA-A2/K
b, and represent potentially invaluable tools to study HLA-A2-restricted anti-tumor immunity in mice.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2004.11.035</identifier><identifier>PMID: 15911111</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Anti-tumor immunity ; Antibody Formation ; Bone cancer ; Cell culture ; Cytomegalovirus ; Cytotoxicity ; Disease Models, Animal ; Experiments ; Female ; HLA-A2 Antigen - immunology ; HLA-A2/K b ; Lung Neoplasms - immunology ; Lung Neoplasms - secondary ; Lung Neoplasms - veterinary ; Lymphocytes ; Male ; MHC class I ; Mice ; Mice, Inbred C57BL ; Mice, Nude ; Mice, Transgenic ; Osteosarcoma - immunology ; Osteosarcoma - veterinary ; Peptides ; Rodents ; Studies ; T cells ; Transgenic animals ; Tumor Cells, Cultured - immunology ; Tumor model ; Tumors</subject><ispartof>Cancer letters, 2005-06, Vol.224 (1), p.153-166</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>Copyright Elsevier Limited Jun 16, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-502cadd96532ad32c989509aeba4210d45816392462b478d750a8a6185a202653</citedby><cites>FETCH-LOGICAL-c334t-502cadd96532ad32c989509aeba4210d45816392462b478d750a8a6185a202653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304383504008894$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15911111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaplan, Barbara L.F.</creatorcontrib><creatorcontrib>Moore, Tamson V.</creatorcontrib><creatorcontrib>Schreiber, Karin</creatorcontrib><creatorcontrib>Callender, Glenda G.</creatorcontrib><creatorcontrib>Schreiber, Hans</creatorcontrib><creatorcontrib>Nishimura, Michael I.</creatorcontrib><title>A new murine tumor model for studying HLA-A2-restricted anti-tumor immunity</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>HLA-A2/K
b transgenic mice have been powerful tools for studying HLA-A2-restricted anti-tumor immunity. Two tumor lines were established from an aged HLA-A2/K
b transgenic mouse that developed spontaneous tumors in the right limb and lung. Histopathologic analysis of the tumor was consistent with an osteosarcoma that had metastasized to the lung. The cells from the primary tumor and the lung metastasis were adapted to culture and are designated Ag201P and Ag201M, respectively. Both Ag201P and Ag201M induced tumors in mice, indicating that the established cell lines are tumorigenic. Both tumor lines expressed HLA-A2/K
b as assessed by RT-PCR and immunofluorescence analysis. Furthermore, the HLA-A2/K
b molecules were functional on both tumor lines as demonstrated by their ability to present exogenously loaded HLA-A2-restricted peptides to human HLA-A2-restricted T cells. More importantly, endogenously expressed HLA-A2-restricted epitopes were processed and presented in the context of HLA-A2/K
b in Ag201P and Ag201M cells to human HLA-A2-restricted T cells. Thus, Ag201P and Ag201M are two new murine tumor lines that express functional HLA-A2/K
b, and represent potentially invaluable tools to study HLA-A2-restricted anti-tumor immunity in mice.</description><subject>Animals</subject><subject>Anti-tumor immunity</subject><subject>Antibody Formation</subject><subject>Bone cancer</subject><subject>Cell culture</subject><subject>Cytomegalovirus</subject><subject>Cytotoxicity</subject><subject>Disease Models, Animal</subject><subject>Experiments</subject><subject>Female</subject><subject>HLA-A2 Antigen - immunology</subject><subject>HLA-A2/K b</subject><subject>Lung Neoplasms - immunology</subject><subject>Lung Neoplasms - secondary</subject><subject>Lung Neoplasms - veterinary</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>MHC class I</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Nude</subject><subject>Mice, Transgenic</subject><subject>Osteosarcoma - immunology</subject><subject>Osteosarcoma - veterinary</subject><subject>Peptides</subject><subject>Rodents</subject><subject>Studies</subject><subject>T cells</subject><subject>Transgenic animals</subject><subject>Tumor Cells, Cultured - immunology</subject><subject>Tumor model</subject><subject>Tumors</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpSbZp_kEphkJudkafli6BJTRNyUIu6VloJW3RYsuJJKfsv68WLxRyaHUZHZ53ZqQHoc8YOgxYXO87a-LgS0cAWIdxB5S_Qysse9L2SsJ7tAIKrKWS8nP0Mec9AHDW8zN0jrnCx7NCD-sm-t_NOKcQfVPmcUrNODk_NLt6y2V2hxB_NfebdbsmbfK5pGCLd42JJbQLH8ZxjqEcPqEPOzNkf3mqF-jn3ben2_t28_j9x-1601pKWWk5EGucU4JTYhwlVknFQRm_NYxgcIxLLKgiTJAt66XrORhpBJbcECA1dYGulr7PaXqZ60p6DNn6YTDRT3PWopcCVE_-C2LFqAQQFfz6BtxPc4r1ERpz4LQHIWml2ELZNOWc_E4_pzCadNAY9NGJ3uvFiT460Rjr6qTGvpyaz9vRu7-hk4QK3CyAr5_2GnzS2QYfrXcheVu0m8K_J_wBuf-b5A</recordid><startdate>20050616</startdate><enddate>20050616</enddate><creator>Kaplan, Barbara L.F.</creator><creator>Moore, Tamson V.</creator><creator>Schreiber, Karin</creator><creator>Callender, Glenda G.</creator><creator>Schreiber, Hans</creator><creator>Nishimura, Michael I.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050616</creationdate><title>A new murine tumor model for studying HLA-A2-restricted anti-tumor immunity</title><author>Kaplan, Barbara L.F. ; Moore, Tamson V. ; Schreiber, Karin ; Callender, Glenda G. ; Schreiber, Hans ; Nishimura, Michael I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-502cadd96532ad32c989509aeba4210d45816392462b478d750a8a6185a202653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Anti-tumor immunity</topic><topic>Antibody Formation</topic><topic>Bone cancer</topic><topic>Cell culture</topic><topic>Cytomegalovirus</topic><topic>Cytotoxicity</topic><topic>Disease Models, Animal</topic><topic>Experiments</topic><topic>Female</topic><topic>HLA-A2 Antigen - immunology</topic><topic>HLA-A2/K b</topic><topic>Lung Neoplasms - immunology</topic><topic>Lung Neoplasms - secondary</topic><topic>Lung Neoplasms - veterinary</topic><topic>Lymphocytes</topic><topic>Male</topic><topic>MHC class I</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Nude</topic><topic>Mice, Transgenic</topic><topic>Osteosarcoma - immunology</topic><topic>Osteosarcoma - veterinary</topic><topic>Peptides</topic><topic>Rodents</topic><topic>Studies</topic><topic>T cells</topic><topic>Transgenic animals</topic><topic>Tumor Cells, Cultured - immunology</topic><topic>Tumor model</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaplan, Barbara L.F.</creatorcontrib><creatorcontrib>Moore, Tamson V.</creatorcontrib><creatorcontrib>Schreiber, Karin</creatorcontrib><creatorcontrib>Callender, Glenda G.</creatorcontrib><creatorcontrib>Schreiber, Hans</creatorcontrib><creatorcontrib>Nishimura, Michael I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaplan, Barbara L.F.</au><au>Moore, Tamson V.</au><au>Schreiber, Karin</au><au>Callender, Glenda G.</au><au>Schreiber, Hans</au><au>Nishimura, Michael I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new murine tumor model for studying HLA-A2-restricted anti-tumor immunity</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2005-06-16</date><risdate>2005</risdate><volume>224</volume><issue>1</issue><spage>153</spage><epage>166</epage><pages>153-166</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>HLA-A2/K
b transgenic mice have been powerful tools for studying HLA-A2-restricted anti-tumor immunity. Two tumor lines were established from an aged HLA-A2/K
b transgenic mouse that developed spontaneous tumors in the right limb and lung. Histopathologic analysis of the tumor was consistent with an osteosarcoma that had metastasized to the lung. The cells from the primary tumor and the lung metastasis were adapted to culture and are designated Ag201P and Ag201M, respectively. Both Ag201P and Ag201M induced tumors in mice, indicating that the established cell lines are tumorigenic. Both tumor lines expressed HLA-A2/K
b as assessed by RT-PCR and immunofluorescence analysis. Furthermore, the HLA-A2/K
b molecules were functional on both tumor lines as demonstrated by their ability to present exogenously loaded HLA-A2-restricted peptides to human HLA-A2-restricted T cells. More importantly, endogenously expressed HLA-A2-restricted epitopes were processed and presented in the context of HLA-A2/K
b in Ag201P and Ag201M cells to human HLA-A2-restricted T cells. Thus, Ag201P and Ag201M are two new murine tumor lines that express functional HLA-A2/K
b, and represent potentially invaluable tools to study HLA-A2-restricted anti-tumor immunity in mice.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>15911111</pmid><doi>10.1016/j.canlet.2004.11.035</doi><tpages>14</tpages></addata></record> |
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| ispartof | Cancer letters, 2005-06, Vol.224 (1), p.153-166 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Anti-tumor immunity Antibody Formation Bone cancer Cell culture Cytomegalovirus Cytotoxicity Disease Models, Animal Experiments Female HLA-A2 Antigen - immunology HLA-A2/K b Lung Neoplasms - immunology Lung Neoplasms - secondary Lung Neoplasms - veterinary Lymphocytes Male MHC class I Mice Mice, Inbred C57BL Mice, Nude Mice, Transgenic Osteosarcoma - immunology Osteosarcoma - veterinary Peptides Rodents Studies T cells Transgenic animals Tumor Cells, Cultured - immunology Tumor model Tumors |
| title | A new murine tumor model for studying HLA-A2-restricted anti-tumor immunity |
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