Western blot immunoassay as a confirmatory test for the presence of anti-Mycoplasma hyopneumoniae antibodies in swine serum
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA. A Western blot immunoassay (WBI) was developed as a confirmatory test for 2 commercial Mycoplasma hyopneumoniae (Mhyo) ELISAs. The WBI detected at least 5 antig...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2006-03, Vol.18 (2), p.198-201 |
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| creator | Ameri, M Zhou, E.M Hsu, W.H |
| description | Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
A Western blot immunoassay (WBI) was developed as a confirmatory test for 2 commercial Mycoplasma hyopneumoniae (Mhyo) ELISAs. The WBI detected at least 5 antigen bands (150, 130, 74, 70, and 30 kDa) from Mhyo whole membrane proteins that were not present in the antigens prepared from M. hyorhinis and M. hyosynoviae. Among discrepant sera from vaccinated pigs (n = 17) and field samples (n = 91) assayed by WBI: 1) 2 of the ELISA-positive samples reacted with all 5 antigens bands; 2) all blocking ELISA-positive samples (n = 53) bound 150-, 130-, 74-, and 70-kD antigen bands; and 3) all indirect ELISA-positive samples (n = 55) bound 150-, 130-, and 30-kD antigens. We conclude that the WBI targeting the top 4 antigen bands is a useful confirmatory test for samples initially screened using the commercial Mhyo ELISAs. |
| doi_str_mv | 10.1177/104063870601800210 |
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A Western blot immunoassay (WBI) was developed as a confirmatory test for 2 commercial Mycoplasma hyopneumoniae (Mhyo) ELISAs. The WBI detected at least 5 antigen bands (150, 130, 74, 70, and 30 kDa) from Mhyo whole membrane proteins that were not present in the antigens prepared from M. hyorhinis and M. hyosynoviae. Among discrepant sera from vaccinated pigs (n = 17) and field samples (n = 91) assayed by WBI: 1) 2 of the ELISA-positive samples reacted with all 5 antigens bands; 2) all blocking ELISA-positive samples (n = 53) bound 150-, 130-, 74-, and 70-kD antigen bands; and 3) all indirect ELISA-positive samples (n = 55) bound 150-, 130-, and 30-kD antigens. We conclude that the WBI targeting the top 4 antigen bands is a useful confirmatory test for samples initially screened using the commercial Mhyo ELISAs.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063870601800210</identifier><identifier>PMID: 16617702</identifier><language>eng</language><publisher>Los Angeles, CA: J Vet Diagn Invest</publisher><subject>Animals ; Antibodies, Bacterial - blood ; antibody detection ; antigen detection ; antiserum ; Blotting, Western - methods ; Blotting, Western - veterinary ; diagnostic techniques ; Mycoplasma hyopneumoniae ; Mycoplasma hyopneumoniae - immunology ; Mycoplasma hyopneumoniae - isolation & purification ; mycoplasmosis ; Pneumonia of Swine, Mycoplasmal - diagnosis ; Pneumonia of Swine, Mycoplasmal - microbiology ; respiratory tract diseases ; Respiratory Tract Infections - diagnosis ; Respiratory Tract Infections - microbiology ; Respiratory Tract Infections - veterinary ; serodiagnosis ; strain differences ; strains ; Swine ; swine diseases ; Western blotting</subject><ispartof>Journal of veterinary diagnostic investigation, 2006-03, Vol.18 (2), p.198-201</ispartof><rights>2006 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-b038c1366d1fcbcf8e088014239daf348d88bbc6916b643a6b471cd9d1ce56a43</citedby><cites>FETCH-LOGICAL-c440t-b038c1366d1fcbcf8e088014239daf348d88bbc6916b643a6b471cd9d1ce56a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/104063870601800210$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/104063870601800210$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21799,27903,27904,43600,43601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16617702$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ameri, M</creatorcontrib><creatorcontrib>Zhou, E.M</creatorcontrib><creatorcontrib>Hsu, W.H</creatorcontrib><title>Western blot immunoassay as a confirmatory test for the presence of anti-Mycoplasma hyopneumoniae antibodies in swine serum</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
A Western blot immunoassay (WBI) was developed as a confirmatory test for 2 commercial Mycoplasma hyopneumoniae (Mhyo) ELISAs. The WBI detected at least 5 antigen bands (150, 130, 74, 70, and 30 kDa) from Mhyo whole membrane proteins that were not present in the antigens prepared from M. hyorhinis and M. hyosynoviae. Among discrepant sera from vaccinated pigs (n = 17) and field samples (n = 91) assayed by WBI: 1) 2 of the ELISA-positive samples reacted with all 5 antigens bands; 2) all blocking ELISA-positive samples (n = 53) bound 150-, 130-, 74-, and 70-kD antigen bands; and 3) all indirect ELISA-positive samples (n = 55) bound 150-, 130-, and 30-kD antigens. We conclude that the WBI targeting the top 4 antigen bands is a useful confirmatory test for samples initially screened using the commercial Mhyo ELISAs.</description><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>antibody detection</subject><subject>antigen detection</subject><subject>antiserum</subject><subject>Blotting, Western - methods</subject><subject>Blotting, Western - veterinary</subject><subject>diagnostic techniques</subject><subject>Mycoplasma hyopneumoniae</subject><subject>Mycoplasma hyopneumoniae - immunology</subject><subject>Mycoplasma hyopneumoniae - isolation & purification</subject><subject>mycoplasmosis</subject><subject>Pneumonia of Swine, Mycoplasmal - diagnosis</subject><subject>Pneumonia of Swine, Mycoplasmal - microbiology</subject><subject>respiratory tract diseases</subject><subject>Respiratory Tract Infections - diagnosis</subject><subject>Respiratory Tract Infections - microbiology</subject><subject>Respiratory Tract Infections - veterinary</subject><subject>serodiagnosis</subject><subject>strain differences</subject><subject>strains</subject><subject>Swine</subject><subject>swine diseases</subject><subject>Western blotting</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhiNERT_gD3AAn7iFzsRexzmiqnxIrTiUiqPlOJNdrxI72EmrqH8el12JAxInj-Rn3pl5iuItwkfEur5EECC5qkECKoAK4UVxho3gpWi4fJnrDJTPxGlxntIeYFNtanxVnKKUOQCqs-LpJ6WZomftEGbmxnHxwaRkVmYSM8wG37s4mjnElc0ZZX2IbN4RmyIl8pZY6JnxsytvVxumwaTRsN0aJk_LGLwz9Oe3DZ2jxJxn6dF5YoniMr4uTnozJHpzfC-K-8_XP66-ljffv3y7-nRTWiFgLlvgyiKXssPetrZXBEoBioo3nem5UJ1SbWtlg7KVghvZihpt13RoaSON4BfFh0PuFMOvJR-hR5csDYPxFJakZa02TTaTweoA2hhSitTrKbrRxFUj6Gfl-l_luendMX1pR-r-thwdZ-DyACSzJb0PS_T52v9HHhfeue3u0UXS2eow5AGV3j90DpWuNDYqg-8PYG-CNtvokr6_qwA5IDRSyIb_BqZlohU</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Ameri, M</creator><creator>Zhou, E.M</creator><creator>Hsu, W.H</creator><general>J Vet Diagn Invest</general><general>SAGE Publications</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>Western blot immunoassay as a confirmatory test for the presence of anti-Mycoplasma hyopneumoniae antibodies in swine serum</title><author>Ameri, M ; Zhou, E.M ; Hsu, W.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-b038c1366d1fcbcf8e088014239daf348d88bbc6916b643a6b471cd9d1ce56a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>antibody detection</topic><topic>antigen detection</topic><topic>antiserum</topic><topic>Blotting, Western - methods</topic><topic>Blotting, Western - veterinary</topic><topic>diagnostic techniques</topic><topic>Mycoplasma hyopneumoniae</topic><topic>Mycoplasma hyopneumoniae - immunology</topic><topic>Mycoplasma hyopneumoniae - isolation & purification</topic><topic>mycoplasmosis</topic><topic>Pneumonia of Swine, Mycoplasmal - diagnosis</topic><topic>Pneumonia of Swine, Mycoplasmal - microbiology</topic><topic>respiratory tract diseases</topic><topic>Respiratory Tract Infections - diagnosis</topic><topic>Respiratory Tract Infections - microbiology</topic><topic>Respiratory Tract Infections - veterinary</topic><topic>serodiagnosis</topic><topic>strain differences</topic><topic>strains</topic><topic>Swine</topic><topic>swine diseases</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ameri, M</creatorcontrib><creatorcontrib>Zhou, E.M</creatorcontrib><creatorcontrib>Hsu, W.H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ameri, M</au><au>Zhou, E.M</au><au>Hsu, W.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Western blot immunoassay as a confirmatory test for the presence of anti-Mycoplasma hyopneumoniae antibodies in swine serum</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>18</volume><issue>2</issue><spage>198</spage><epage>201</epage><pages>198-201</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
A Western blot immunoassay (WBI) was developed as a confirmatory test for 2 commercial Mycoplasma hyopneumoniae (Mhyo) ELISAs. The WBI detected at least 5 antigen bands (150, 130, 74, 70, and 30 kDa) from Mhyo whole membrane proteins that were not present in the antigens prepared from M. hyorhinis and M. hyosynoviae. Among discrepant sera from vaccinated pigs (n = 17) and field samples (n = 91) assayed by WBI: 1) 2 of the ELISA-positive samples reacted with all 5 antigens bands; 2) all blocking ELISA-positive samples (n = 53) bound 150-, 130-, 74-, and 70-kD antigen bands; and 3) all indirect ELISA-positive samples (n = 55) bound 150-, 130-, and 30-kD antigens. We conclude that the WBI targeting the top 4 antigen bands is a useful confirmatory test for samples initially screened using the commercial Mhyo ELISAs.</abstract><cop>Los Angeles, CA</cop><pub>J Vet Diagn Invest</pub><pmid>16617702</pmid><doi>10.1177/104063870601800210</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies, Bacterial - blood antibody detection antigen detection antiserum Blotting, Western - methods Blotting, Western - veterinary diagnostic techniques Mycoplasma hyopneumoniae Mycoplasma hyopneumoniae - immunology Mycoplasma hyopneumoniae - isolation & purification mycoplasmosis Pneumonia of Swine, Mycoplasmal - diagnosis Pneumonia of Swine, Mycoplasmal - microbiology respiratory tract diseases Respiratory Tract Infections - diagnosis Respiratory Tract Infections - microbiology Respiratory Tract Infections - veterinary serodiagnosis strain differences strains Swine swine diseases Western blotting |
| title | Western blot immunoassay as a confirmatory test for the presence of anti-Mycoplasma hyopneumoniae antibodies in swine serum |
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