Boophilus microplus Infection by Beauveria amorpha and Beauveria bassiana: SEM Analysis and Regulation of Subtilisin-like Proteases and Chitinases
Beauveria bassiana is a well-known broad-range arthropod pathogen which has been used in biological control of several pest insects and ticks such as Boophilus microplus. Beauveria amorpha has both endophytic and entomopathogenic characteristics, but its capacity for biological control has still not...
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| Veröffentlicht in: | Current microbiology 2005-05, Vol.50 (5), p.257-261 |
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| container_title | Current microbiology |
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| creator | Campos, R.A Arruda, W Boldo, J.T Silva, M.V. da Barros, N.M. de Azevedo, J.L. de Schrank, A Vainstein, M.H |
| description | Beauveria bassiana is a well-known broad-range arthropod pathogen which has been used in biological control of several pest insects and ticks such as Boophilus microplus. Beauveria amorpha has both endophytic and entomopathogenic characteristics, but its capacity for biological control has still not been studied. During the processes of host infection, B. bassiana and B. amorpha produce several hydrolytic extracellular enzymes, including proteases and chitinases, which probably degrade the host cuticle and are suggested to be pathogenicity determinants. To access the role of these enzymes during infection in the tick B. microplus, we analyzed their secretion during fungus growth in single and combined carbon sources, compared to complex substrates such as chitin and B. microplus cuticle. Chitin and tick cuticle-induced chitinase in both fungus and protease was induced only by tick cuticle. SEM analysis of B. amorpha and B. bassiana infecting B. microplus showed apressorium formation during penetration on cattle tick cuticle. |
| doi_str_mv | 10.1007/s00284-004-4460-y |
| format | Article |
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Beauveria amorpha has both endophytic and entomopathogenic characteristics, but its capacity for biological control has still not been studied. During the processes of host infection, B. bassiana and B. amorpha produce several hydrolytic extracellular enzymes, including proteases and chitinases, which probably degrade the host cuticle and are suggested to be pathogenicity determinants. To access the role of these enzymes during infection in the tick B. microplus, we analyzed their secretion during fungus growth in single and combined carbon sources, compared to complex substrates such as chitin and B. microplus cuticle. Chitin and tick cuticle-induced chitinase in both fungus and protease was induced only by tick cuticle. SEM analysis of B. amorpha and B. bassiana infecting B. microplus showed apressorium formation during penetration on cattle tick cuticle.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-004-4460-y</identifier><identifier>PMID: 15886912</identifier><language>eng</language><publisher>United States</publisher><subject>Amorpha ; animal pathogenic fungi ; Animals ; Arthropoda ; Ascomycota - enzymology ; Ascomycota - isolation & purification ; Ascomycota - ultrastructure ; Beauveria ; Beauveria amorpha ; Beauveria bassiana ; biological control ; biological control agents ; Boophilus microplus ; cattle diseases ; Chitin - metabolism ; chitinase ; Chitinases - metabolism ; Ixodidae ; Peptide Hydrolases - metabolism ; Pest Control ; proteinases ; scanning electron microscopes ; subtilisin ; tick infestations ; ticks ; Ticks - microbiology ; Ticks - ultrastructure</subject><ispartof>Current microbiology, 2005-05, Vol.50 (5), p.257-261</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15886912$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Campos, R.A</creatorcontrib><creatorcontrib>Arruda, W</creatorcontrib><creatorcontrib>Boldo, J.T</creatorcontrib><creatorcontrib>Silva, M.V. da</creatorcontrib><creatorcontrib>Barros, N.M. de</creatorcontrib><creatorcontrib>Azevedo, J.L. de</creatorcontrib><creatorcontrib>Schrank, A</creatorcontrib><creatorcontrib>Vainstein, M.H</creatorcontrib><title>Boophilus microplus Infection by Beauveria amorpha and Beauveria bassiana: SEM Analysis and Regulation of Subtilisin-like Proteases and Chitinases</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>Beauveria bassiana is a well-known broad-range arthropod pathogen which has been used in biological control of several pest insects and ticks such as Boophilus microplus. Beauveria amorpha has both endophytic and entomopathogenic characteristics, but its capacity for biological control has still not been studied. During the processes of host infection, B. bassiana and B. amorpha produce several hydrolytic extracellular enzymes, including proteases and chitinases, which probably degrade the host cuticle and are suggested to be pathogenicity determinants. To access the role of these enzymes during infection in the tick B. microplus, we analyzed their secretion during fungus growth in single and combined carbon sources, compared to complex substrates such as chitin and B. microplus cuticle. Chitin and tick cuticle-induced chitinase in both fungus and protease was induced only by tick cuticle. SEM analysis of B. amorpha and B. bassiana infecting B. microplus showed apressorium formation during penetration on cattle tick cuticle.</description><subject>Amorpha</subject><subject>animal pathogenic fungi</subject><subject>Animals</subject><subject>Arthropoda</subject><subject>Ascomycota - enzymology</subject><subject>Ascomycota - isolation & purification</subject><subject>Ascomycota - ultrastructure</subject><subject>Beauveria</subject><subject>Beauveria amorpha</subject><subject>Beauveria bassiana</subject><subject>biological control</subject><subject>biological control agents</subject><subject>Boophilus microplus</subject><subject>cattle diseases</subject><subject>Chitin - metabolism</subject><subject>chitinase</subject><subject>Chitinases - metabolism</subject><subject>Ixodidae</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Pest Control</subject><subject>proteinases</subject><subject>scanning electron microscopes</subject><subject>subtilisin</subject><subject>tick infestations</subject><subject>ticks</subject><subject>Ticks - microbiology</subject><subject>Ticks - ultrastructure</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M1O3DAQB3ALUcGW9gF6aXPi5nb8Ea_dG6xoiwQCdcs5mjgT1pDEIU6Q9jX6xASWSr1xmv_M_DSHYeyTgK8CYPktAUirOYDmWhvg2z22EFpJDs6JfbYApRW3JheH7H1KdwBCOhAH7FDk1hon5IL9PY2x34RmSlkb_BD753Te1eTHELus3GanhNMjDQEzbOPQb-baVf9NS0wpYIffs_XZZXbSYbNNIb2g33Q7NfhyKNbZeirH0IQUOt6Ee8quhzgSJtrZ1SaMoXtuP7B3NTaJPr7WI3bz4-zP6he_uPp5vjq54LU0euSIVHuQwtS-LLWuq8qjxEop52mpyCg_C6EFCAeOTOksKZdrX0nhDYmlOmLHu7v9EB8mSmPRhuSpabCjOKXCLG1ucy3fhBKsUlLZGX5-hVPZUlX0Q2hx2Bb_3j2DLztQYyzwdgipuFlLEAoEzPs5PAHZdI6R</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Campos, R.A</creator><creator>Arruda, W</creator><creator>Boldo, J.T</creator><creator>Silva, M.V. da</creator><creator>Barros, N.M. de</creator><creator>Azevedo, J.L. de</creator><creator>Schrank, A</creator><creator>Vainstein, M.H</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7SS</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Boophilus microplus Infection by Beauveria amorpha and Beauveria bassiana: SEM Analysis and Regulation of Subtilisin-like Proteases and Chitinases</title><author>Campos, R.A ; 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Beauveria amorpha has both endophytic and entomopathogenic characteristics, but its capacity for biological control has still not been studied. During the processes of host infection, B. bassiana and B. amorpha produce several hydrolytic extracellular enzymes, including proteases and chitinases, which probably degrade the host cuticle and are suggested to be pathogenicity determinants. To access the role of these enzymes during infection in the tick B. microplus, we analyzed their secretion during fungus growth in single and combined carbon sources, compared to complex substrates such as chitin and B. microplus cuticle. Chitin and tick cuticle-induced chitinase in both fungus and protease was induced only by tick cuticle. SEM analysis of B. amorpha and B. bassiana infecting B. microplus showed apressorium formation during penetration on cattle tick cuticle.</abstract><cop>United States</cop><pmid>15886912</pmid><doi>10.1007/s00284-004-4460-y</doi><tpages>5</tpages></addata></record> |
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| ispartof | Current microbiology, 2005-05, Vol.50 (5), p.257-261 |
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| subjects | Amorpha animal pathogenic fungi Animals Arthropoda Ascomycota - enzymology Ascomycota - isolation & purification Ascomycota - ultrastructure Beauveria Beauveria amorpha Beauveria bassiana biological control biological control agents Boophilus microplus cattle diseases Chitin - metabolism chitinase Chitinases - metabolism Ixodidae Peptide Hydrolases - metabolism Pest Control proteinases scanning electron microscopes subtilisin tick infestations ticks Ticks - microbiology Ticks - ultrastructure |
| title | Boophilus microplus Infection by Beauveria amorpha and Beauveria bassiana: SEM Analysis and Regulation of Subtilisin-like Proteases and Chitinases |
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