Fluorescence-Detection Size-Exclusion Chromatography for Precrystallization Screening of Integral Membrane Proteins

Formation of well-ordered crystals of membrane proteins is a bottleneck for structure determination by X-ray crystallography. Nevertheless, one can increase the probability of successful crystallization by precrystallization screening, a process by which one analyzes the monodispersity and stability...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Structure (London) 2006-04, Vol.14 (4), p.673-681
Hauptverfasser:	Kawate, Toshimitsu, Gouaux, Eric
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins - chemistry Cell Line Chromatography, Gel - methods Crystallography, X-Ray Detergents - pharmacology Fluorescent Dyes - pharmacology Genetic Vectors Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - metabolism Humans Membrane Proteins - chemistry Neurotransmitter Agents - metabolism Normal Distribution Plasmids - metabolism Proteins - chemistry Receptors, Purinergic P2 - chemistry Receptors, Purinergic P2X Time Factors
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!