Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2
BMP signaling plays pleiotropic roles in various tissues. Transgenic mouse lines that overexpress BMP signaling in a tissue‐specific manner would be beneficial; however, production of each tissue‐specific transgenic mouse line is labor‐intensive. Here, using a Cre‐loxP system, we generated a conditi...
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| Veröffentlicht in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2006-04, Vol.44 (4), p.159-167 |
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| container_title | Genesis (New York, N.Y. : 2000) |
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| creator | Fukuda, Tomokazu Scott, Gregory Komatsu, Yoshihiro Araya, Runa Kawano, Masako Ray, Manas K. Yamada, Masahisa Mishina, Yuji |
| description | BMP signaling plays pleiotropic roles in various tissues. Transgenic mouse lines that overexpress BMP signaling in a tissue‐specific manner would be beneficial; however, production of each tissue‐specific transgenic mouse line is labor‐intensive. Here, using a Cre‐loxP system, we generated a conditionally overexpressing mouse line for BMP signaling through the type I receptor ALK2 (alternatively known as AVCRI, ActRI, or ActRIA). By mating this line with Cre‐expression mouse lines, Cre‐mediated recombination removes an intervening floxed lacZ expression cassette and thereby permits the expression of a constitutively active form of Alk2 (caAlk2) driven by a ubiquitous promoter, CAG. Tissue specificity of Cre recombination was monitored by a bicistronically expressed EGFP following Alk2 cDNA. Increased BMP signaling was confirmed by ectopic phosphorylation of SMAD1/5/8 in the areas where Cre recombination had occurred. The conditional overexpression system described here provides versatility in investigating gene functions in a tissue‐specific manner without having to generate independent tissue‐specific transgenic lines. genesis 44:159–167, 2006. Published 2006 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/dvg.20201 |
| format | Article |
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Transgenic mouse lines that overexpress BMP signaling in a tissue‐specific manner would be beneficial; however, production of each tissue‐specific transgenic mouse line is labor‐intensive. Here, using a Cre‐loxP system, we generated a conditionally overexpressing mouse line for BMP signaling through the type I receptor ALK2 (alternatively known as AVCRI, ActRI, or ActRIA). By mating this line with Cre‐expression mouse lines, Cre‐mediated recombination removes an intervening floxed lacZ expression cassette and thereby permits the expression of a constitutively active form of Alk2 (caAlk2) driven by a ubiquitous promoter, CAG. Tissue specificity of Cre recombination was monitored by a bicistronically expressed EGFP following Alk2 cDNA. Increased BMP signaling was confirmed by ectopic phosphorylation of SMAD1/5/8 in the areas where Cre recombination had occurred. The conditional overexpression system described here provides versatility in investigating gene functions in a tissue‐specific manner without having to generate independent tissue‐specific transgenic lines. genesis 44:159–167, 2006. 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Transgenic mouse lines that overexpress BMP signaling in a tissue‐specific manner would be beneficial; however, production of each tissue‐specific transgenic mouse line is labor‐intensive. Here, using a Cre‐loxP system, we generated a conditionally overexpressing mouse line for BMP signaling through the type I receptor ALK2 (alternatively known as AVCRI, ActRI, or ActRIA). By mating this line with Cre‐expression mouse lines, Cre‐mediated recombination removes an intervening floxed lacZ expression cassette and thereby permits the expression of a constitutively active form of Alk2 (caAlk2) driven by a ubiquitous promoter, CAG. Tissue specificity of Cre recombination was monitored by a bicistronically expressed EGFP following Alk2 cDNA. Increased BMP signaling was confirmed by ectopic phosphorylation of SMAD1/5/8 in the areas where Cre recombination had occurred. The conditional overexpression system described here provides versatility in investigating gene functions in a tissue‐specific manner without having to generate independent tissue‐specific transgenic lines. genesis 44:159–167, 2006. Published 2006 Wiley‐Liss, Inc.</description><subject>Activin Receptors, Type I - genetics</subject><subject>Activin Receptors, Type I - metabolism</subject><subject>Adenoviridae - genetics</subject><subject>Animals</subject><subject>bone morphogenetic protein</subject><subject>Bone Morphogenetic Protein Receptors - metabolism</subject><subject>constitutively active</subject><subject>Cre-loxP system</subject><subject>Crosses, Genetic</subject><subject>Female</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Integrases - genetics</subject><subject>Integrases - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Models, Genetic</subject><subject>Promoter Regions, Genetic</subject><subject>Recombination, Genetic</subject><subject>Signal Transduction</subject><subject>Smad</subject><subject>Transgenes</subject><subject>transgenic mouse</subject><issn>1526-954X</issn><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9PGzEQxS1UBDT00C9Q-YSExILHu_53JEADIi2VWigSB8vxehOXzW6wN4R8e0wT6An19EYzv3nSewh9BnIIhNCj8nF8SAklsIF2gFGeKS5vP7zOrLjdRh9j_EMIYZLSLbQNnJOCgdxBdwPXuGA63za4rbDB03YeHV74boJt25T-5WLqeomN7fyj6VyJox-nlW_GuJuEdj6eJHW4_-0HDs66WdeGA3w8vKS7aLMydXSf1tpD11_Pfp2cZ8OrwcXJ8TCzuaKQCVGplIJYxhV1Mh-pkaJCyspSLkVecA5EMF6yopIsbRhjtqRlqUACAcHyHtpb-c5C-zB3sdNTH62ra9O4lEZzIRlTUv0XBAEFVTkkcH8F2tDGGFylZ8FPTVhqIPqlcp0q138rT-yXtel8NHXlP3LdcQKOVsDC1275vpM-vRm8WmarDx879_T2YcJ9ypILpn9_H2joK_ipFNWQPwNTvZcT</recordid><startdate>200604</startdate><enddate>200604</enddate><creator>Fukuda, Tomokazu</creator><creator>Scott, Gregory</creator><creator>Komatsu, Yoshihiro</creator><creator>Araya, Runa</creator><creator>Kawano, Masako</creator><creator>Ray, Manas K.</creator><creator>Yamada, Masahisa</creator><creator>Mishina, Yuji</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200604</creationdate><title>Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2</title><author>Fukuda, Tomokazu ; 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Transgenic mouse lines that overexpress BMP signaling in a tissue‐specific manner would be beneficial; however, production of each tissue‐specific transgenic mouse line is labor‐intensive. Here, using a Cre‐loxP system, we generated a conditionally overexpressing mouse line for BMP signaling through the type I receptor ALK2 (alternatively known as AVCRI, ActRI, or ActRIA). By mating this line with Cre‐expression mouse lines, Cre‐mediated recombination removes an intervening floxed lacZ expression cassette and thereby permits the expression of a constitutively active form of Alk2 (caAlk2) driven by a ubiquitous promoter, CAG. Tissue specificity of Cre recombination was monitored by a bicistronically expressed EGFP following Alk2 cDNA. Increased BMP signaling was confirmed by ectopic phosphorylation of SMAD1/5/8 in the areas where Cre recombination had occurred. 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| subjects | Activin Receptors, Type I - genetics Activin Receptors, Type I - metabolism Adenoviridae - genetics Animals bone morphogenetic protein Bone Morphogenetic Protein Receptors - metabolism constitutively active Cre-loxP system Crosses, Genetic Female Gene Expression Regulation, Developmental Green Fluorescent Proteins - metabolism Integrases - genetics Integrases - metabolism Male Mice Mice, Transgenic Models, Genetic Promoter Regions, Genetic Recombination, Genetic Signal Transduction Smad Transgenes transgenic mouse |
| title | Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2 |
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