Androgenic and antiandrogenic effects and expression of androgen receptor in mouse embryonic stem cells
To investigate the effects of androgen and antiandrogen and the expression of androgen receptor on mouse embryonic stem cells (ESCs) and the inner cell mass. Controlled laboratory study. Academic university hospital. Blastocysts from mice developed at the Institute for Cancer Research and 129/Sv mic...
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| Veröffentlicht in: | Fertility and sterility 2006-04, Vol.85, p.1195-1203 |
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| container_title | Fertility and sterility |
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| creator | Chang, Chih-Yang Hsuuw, Yan-Der Huang, Fu-Jen Shyr, Chih-Rong Chang, Shiuh-Young Huang, Chiung-Kuei Kang, Hong-Yo Huang, Ko-En |
| description | To investigate the effects of androgen and antiandrogen and the expression of androgen receptor on mouse embryonic stem cells (ESCs) and the inner cell mass.
Controlled laboratory study.
Academic university hospital.
Blastocysts from mice developed at the Institute for Cancer Research and 129/Sv mice embryonic stem cell line.
Cultured mouse ESCs were exposed to testosterone (T), dihydrotestosterone (DHT), or the antiandrogen nilutamide.
Immunohistochemistry for androgen receptor (AR), quantitative real-time polymerase chain reaction analysis, cell colorimetric assays, and Western blot analysis.
Androgen receptor messenger RNA (mRNA) was first detected both in the inner cell mass from blastocysts and in undifferentiated ESCs. It increased stage-dependently during ESC differentiation. Although both T and DHT had marginal effects on AR mRNA expression level and cell growth in vitro, the nonsteroidal antiandrogen nilutamide significantly stimulated ESC growth and induced Akt expression. The enhancing effects of nilutamide on mouse ESCs indicated that the Akt pathway may be involved in nilutamide-promoted ESC growth.
These findings provide the first evidence of the existence of AR in ESCs. During differentiation, the expression level of AR was increased in a stage-dependent but not a ligand-dependent manner. Nilutamide promoted cell growth and increased Akt expression in ESCs. |
| doi_str_mv | 10.1016/j.fertnstert.2005.11.031 |
| format | Article |
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Controlled laboratory study.
Academic university hospital.
Blastocysts from mice developed at the Institute for Cancer Research and 129/Sv mice embryonic stem cell line.
Cultured mouse ESCs were exposed to testosterone (T), dihydrotestosterone (DHT), or the antiandrogen nilutamide.
Immunohistochemistry for androgen receptor (AR), quantitative real-time polymerase chain reaction analysis, cell colorimetric assays, and Western blot analysis.
Androgen receptor messenger RNA (mRNA) was first detected both in the inner cell mass from blastocysts and in undifferentiated ESCs. It increased stage-dependently during ESC differentiation. Although both T and DHT had marginal effects on AR mRNA expression level and cell growth in vitro, the nonsteroidal antiandrogen nilutamide significantly stimulated ESC growth and induced Akt expression. The enhancing effects of nilutamide on mouse ESCs indicated that the Akt pathway may be involved in nilutamide-promoted ESC growth.
These findings provide the first evidence of the existence of AR in ESCs. During differentiation, the expression level of AR was increased in a stage-dependent but not a ligand-dependent manner. Nilutamide promoted cell growth and increased Akt expression in ESCs.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2005.11.031</identifier><identifier>PMID: 16616092</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Androgen Antagonists - pharmacology ; Androgen receptor ; Androgens - administration & dosage ; Animals ; Biological and medical sciences ; Blastocyst - cytology ; Blastocyst - drug effects ; Blastocyst - metabolism ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; dihydrotestosterone ; Dihydrotestosterone - pharmacology ; Gene Expression Regulation, Developmental - drug effects ; Gene Expression Regulation, Developmental - physiology ; Gynecology. Andrology. Obstetrics ; Imidazolidines - pharmacology ; Medical sciences ; Mice ; Mice, Inbred ICR ; mouse embryonic stem cell ; nilutamide ; proliferation ; Receptors, Adrenergic - drug effects ; Receptors, Adrenergic - metabolism ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - metabolism ; testosterone ; Testosterone - pharmacology</subject><ispartof>Fertility and sterility, 2006-04, Vol.85, p.1195-1203</ispartof><rights>2006 American Society for Reproductive Medicine</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-8117ca65f8b6ee422e5cb4cfde8af5c5f7c5a0d249f8d8059f2061ffce47cf7a3</citedby><cites>FETCH-LOGICAL-c518t-8117ca65f8b6ee422e5cb4cfde8af5c5f7c5a0d249f8d8059f2061ffce47cf7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fertnstert.2005.11.031$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17678768$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16616092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Chih-Yang</creatorcontrib><creatorcontrib>Hsuuw, Yan-Der</creatorcontrib><creatorcontrib>Huang, Fu-Jen</creatorcontrib><creatorcontrib>Shyr, Chih-Rong</creatorcontrib><creatorcontrib>Chang, Shiuh-Young</creatorcontrib><creatorcontrib>Huang, Chiung-Kuei</creatorcontrib><creatorcontrib>Kang, Hong-Yo</creatorcontrib><creatorcontrib>Huang, Ko-En</creatorcontrib><title>Androgenic and antiandrogenic effects and expression of androgen receptor in mouse embryonic stem cells</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>To investigate the effects of androgen and antiandrogen and the expression of androgen receptor on mouse embryonic stem cells (ESCs) and the inner cell mass.
Controlled laboratory study.
Academic university hospital.
Blastocysts from mice developed at the Institute for Cancer Research and 129/Sv mice embryonic stem cell line.
Cultured mouse ESCs were exposed to testosterone (T), dihydrotestosterone (DHT), or the antiandrogen nilutamide.
Immunohistochemistry for androgen receptor (AR), quantitative real-time polymerase chain reaction analysis, cell colorimetric assays, and Western blot analysis.
Androgen receptor messenger RNA (mRNA) was first detected both in the inner cell mass from blastocysts and in undifferentiated ESCs. It increased stage-dependently during ESC differentiation. Although both T and DHT had marginal effects on AR mRNA expression level and cell growth in vitro, the nonsteroidal antiandrogen nilutamide significantly stimulated ESC growth and induced Akt expression. The enhancing effects of nilutamide on mouse ESCs indicated that the Akt pathway may be involved in nilutamide-promoted ESC growth.
These findings provide the first evidence of the existence of AR in ESCs. During differentiation, the expression level of AR was increased in a stage-dependent but not a ligand-dependent manner. Nilutamide promoted cell growth and increased Akt expression in ESCs.</description><subject>Androgen Antagonists - pharmacology</subject><subject>Androgen receptor</subject><subject>Androgens - administration & dosage</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>dihydrotestosterone</subject><subject>Dihydrotestosterone - pharmacology</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Imidazolidines - pharmacology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>mouse embryonic stem cell</subject><subject>nilutamide</subject><subject>proliferation</subject><subject>Receptors, Adrenergic - drug effects</subject><subject>Receptors, Adrenergic - metabolism</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><subject>testosterone</subject><subject>Testosterone - pharmacology</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFPHCEYhkmjqavtX2i41NuMfOzAsEdrtJqYeLFnwn7zYdjswBZmG_33su42e_QAJPC88PIwxkG0IEBfrVpPeYplqnMrhVAtQCvm8IXNQCndKK3mJ2wmBKhGSCPP2HkpKyGEhl5-ZWegNWixkDP2ch2HnF4oBuQuDnVMwR23yHvCqXwc0esmUykhRZ48_w_xTEibKWUeIh_TthCncZnf0i5eC44cab0u39ipd-tC3w_rBftzd_t8c988Pv1-uLl-bFCBmRoD0KPTypulJuqkJIXLDv1AxnmFyveonBhkt_BmMEItvKx_8h6p69H3bn7BLvf3bnL6u6Uy2TGUXQMXqZazujeqg25RQbMHMadSMnm7yWF0-c2CsDvJdmWPku1OsgWwVXKN_ji8sV2ONByDB6sV-HkAXEG39tlFDOXI9bVFr03lfu05qkb-Bcq2YKCINIRqdbJDCp-3eQdRyqNb</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Chang, Chih-Yang</creator><creator>Hsuuw, Yan-Der</creator><creator>Huang, Fu-Jen</creator><creator>Shyr, Chih-Rong</creator><creator>Chang, Shiuh-Young</creator><creator>Huang, Chiung-Kuei</creator><creator>Kang, Hong-Yo</creator><creator>Huang, Ko-En</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Androgenic and antiandrogenic effects and expression of androgen receptor in mouse embryonic stem cells</title><author>Chang, Chih-Yang ; Hsuuw, Yan-Der ; Huang, Fu-Jen ; Shyr, Chih-Rong ; Chang, Shiuh-Young ; Huang, Chiung-Kuei ; Kang, Hong-Yo ; Huang, Ko-En</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-8117ca65f8b6ee422e5cb4cfde8af5c5f7c5a0d249f8d8059f2061ffce47cf7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Androgen Antagonists - pharmacology</topic><topic>Androgen receptor</topic><topic>Androgens - administration & dosage</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>dihydrotestosterone</topic><topic>Dihydrotestosterone - pharmacology</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Imidazolidines - pharmacology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>mouse embryonic stem cell</topic><topic>nilutamide</topic><topic>proliferation</topic><topic>Receptors, Adrenergic - drug effects</topic><topic>Receptors, Adrenergic - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><topic>testosterone</topic><topic>Testosterone - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Chih-Yang</creatorcontrib><creatorcontrib>Hsuuw, Yan-Der</creatorcontrib><creatorcontrib>Huang, Fu-Jen</creatorcontrib><creatorcontrib>Shyr, Chih-Rong</creatorcontrib><creatorcontrib>Chang, Shiuh-Young</creatorcontrib><creatorcontrib>Huang, Chiung-Kuei</creatorcontrib><creatorcontrib>Kang, Hong-Yo</creatorcontrib><creatorcontrib>Huang, Ko-En</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Chih-Yang</au><au>Hsuuw, Yan-Der</au><au>Huang, Fu-Jen</au><au>Shyr, Chih-Rong</au><au>Chang, Shiuh-Young</au><au>Huang, Chiung-Kuei</au><au>Kang, Hong-Yo</au><au>Huang, Ko-En</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Androgenic and antiandrogenic effects and expression of androgen receptor in mouse embryonic stem cells</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>85</volume><spage>1195</spage><epage>1203</epage><pages>1195-1203</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>To investigate the effects of androgen and antiandrogen and the expression of androgen receptor on mouse embryonic stem cells (ESCs) and the inner cell mass.
Controlled laboratory study.
Academic university hospital.
Blastocysts from mice developed at the Institute for Cancer Research and 129/Sv mice embryonic stem cell line.
Cultured mouse ESCs were exposed to testosterone (T), dihydrotestosterone (DHT), or the antiandrogen nilutamide.
Immunohistochemistry for androgen receptor (AR), quantitative real-time polymerase chain reaction analysis, cell colorimetric assays, and Western blot analysis.
Androgen receptor messenger RNA (mRNA) was first detected both in the inner cell mass from blastocysts and in undifferentiated ESCs. It increased stage-dependently during ESC differentiation. Although both T and DHT had marginal effects on AR mRNA expression level and cell growth in vitro, the nonsteroidal antiandrogen nilutamide significantly stimulated ESC growth and induced Akt expression. The enhancing effects of nilutamide on mouse ESCs indicated that the Akt pathway may be involved in nilutamide-promoted ESC growth.
These findings provide the first evidence of the existence of AR in ESCs. During differentiation, the expression level of AR was increased in a stage-dependent but not a ligand-dependent manner. Nilutamide promoted cell growth and increased Akt expression in ESCs.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16616092</pmid><doi>10.1016/j.fertnstert.2005.11.031</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Fertility and sterility, 2006-04, Vol.85, p.1195-1203 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Androgen Antagonists - pharmacology Androgen receptor Androgens - administration & dosage Animals Biological and medical sciences Blastocyst - cytology Blastocyst - drug effects Blastocyst - metabolism Cell Differentiation - drug effects Cell Proliferation - drug effects Cells, Cultured dihydrotestosterone Dihydrotestosterone - pharmacology Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Developmental - physiology Gynecology. Andrology. Obstetrics Imidazolidines - pharmacology Medical sciences Mice Mice, Inbred ICR mouse embryonic stem cell nilutamide proliferation Receptors, Adrenergic - drug effects Receptors, Adrenergic - metabolism Stem Cells - cytology Stem Cells - drug effects Stem Cells - metabolism testosterone Testosterone - pharmacology |
| title | Androgenic and antiandrogenic effects and expression of androgen receptor in mouse embryonic stem cells |
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