Quantitative Analysis of Human Telomerase Reverse Transcriptase in Pancreatic Cancer
Although telomerase activity is a promising diagnostic marker, clinical introduction of this marker for cancer diagnosis is still problematic due to the lack of means of evaluating sample quality. Human telomerase reverse transcriptase (hTERT), one of the subunits of telomerase, is also a promising...
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| Veröffentlicht in: | Clinical cancer research 2006-04, Vol.12 (7), p.2066-2069 |
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| creator | OHUCHIDA, Kenoki MIZUMOTO, Kazuhiro YAMADA, Daisuke YAMAGUCHI, Hiroshi KONOMI, Hiroyuki NAGAI, Eishi YAMAGUCHI, Koji TSUNEYOSHI, Masazumi TANAKA, Masao |
| description | Although telomerase activity is a promising diagnostic marker, clinical introduction of this marker for cancer diagnosis is
still problematic due to the lack of means of evaluating sample quality. Human telomerase reverse transcriptase (hTERT), one
of the subunits of telomerase, is also a promising diagnostic marker. In the present study, we did large-scale analysis of
88 pancreatic juice samples to determine the feasibility of quantitative analysis of hTERT mRNA for diagnosis of pancreatic
cancer. We found significant differences in hTERT expression among carcinoma-derived, intraductal papillary mucinous neoplasm
(IPMN)–derived, and chronic pancreatitis–derived juice samples. Results showed that quantitative analyses of hTERT mRNAs are
more useful in discriminating carcinoma from IPMN than from chronic pancreatitis. When the specificity was set at 100%, the
sensitivity for differentiation between carcinoma and IPMN was 43.5%, whereas the sensitivity of cytologic examination was
22.0%. There were significant differences in hTERT expression among carcinoma cells, IPMN cells, and normal ductal cells isolated
from pancreatic tissues by microdissection. Lymphocytes and hyperplastic epithelial cells isolated from tissues with the histologic
appearance of pancreatitis showed various expression levels of hTERT. Our results suggest that quantitative analysis of hTERT
mRNA in pancreatic juice is advantageous over cytologic analysis for differentiation between carcinoma and IPMN but probably
not for differentiation between carcinoma and chronic pancreatitis. |
| doi_str_mv | 10.1158/1078-0432.CCR-05-1821 |
| format | Article |
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still problematic due to the lack of means of evaluating sample quality. Human telomerase reverse transcriptase (hTERT), one
of the subunits of telomerase, is also a promising diagnostic marker. In the present study, we did large-scale analysis of
88 pancreatic juice samples to determine the feasibility of quantitative analysis of hTERT mRNA for diagnosis of pancreatic
cancer. We found significant differences in hTERT expression among carcinoma-derived, intraductal papillary mucinous neoplasm
(IPMN)–derived, and chronic pancreatitis–derived juice samples. Results showed that quantitative analyses of hTERT mRNAs are
more useful in discriminating carcinoma from IPMN than from chronic pancreatitis. When the specificity was set at 100%, the
sensitivity for differentiation between carcinoma and IPMN was 43.5%, whereas the sensitivity of cytologic examination was
22.0%. There were significant differences in hTERT expression among carcinoma cells, IPMN cells, and normal ductal cells isolated
from pancreatic tissues by microdissection. Lymphocytes and hyperplastic epithelial cells isolated from tissues with the histologic
appearance of pancreatitis showed various expression levels of hTERT. Our results suggest that quantitative analysis of hTERT
mRNA in pancreatic juice is advantageous over cytologic analysis for differentiation between carcinoma and IPMN but probably
not for differentiation between carcinoma and chronic pancreatitis.</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>DOI: 10.1158/1078-0432.CCR-05-1821</identifier><identifier>PMID: 16609017</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Antineoplastic agents ; Biological and medical sciences ; chronic pancreatitis ; DNA-Binding Proteins - analysis ; DNA-Binding Proteins - genetics ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene Expression Profiling ; Gene Expression Regulation, Enzymologic - genetics ; Gene Expression Regulation, Neoplastic - genetics ; hTERT ; Humans ; IPMN ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Medical sciences ; pancreatic cancer ; pancreatic juice ; Pancreatic Neoplasms - enzymology ; Pancreatic Neoplasms - genetics ; Pharmacology. Drug treatments ; RNA, Messenger - genetics ; Telomerase - analysis ; Telomerase - genetics ; Tumors</subject><ispartof>Clinical cancer research, 2006-04, Vol.12 (7), p.2066-2069</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-960d2bb9ef64764f9d6776acea398558810da56e08eceff8e1deb06b572c28463</citedby><cites>FETCH-LOGICAL-c467t-960d2bb9ef64764f9d6776acea398558810da56e08eceff8e1deb06b572c28463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3354,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17697469$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16609017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OHUCHIDA, Kenoki</creatorcontrib><creatorcontrib>MIZUMOTO, Kazuhiro</creatorcontrib><creatorcontrib>YAMADA, Daisuke</creatorcontrib><creatorcontrib>YAMAGUCHI, Hiroshi</creatorcontrib><creatorcontrib>KONOMI, Hiroyuki</creatorcontrib><creatorcontrib>NAGAI, Eishi</creatorcontrib><creatorcontrib>YAMAGUCHI, Koji</creatorcontrib><creatorcontrib>TSUNEYOSHI, Masazumi</creatorcontrib><creatorcontrib>TANAKA, Masao</creatorcontrib><title>Quantitative Analysis of Human Telomerase Reverse Transcriptase in Pancreatic Cancer</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>Although telomerase activity is a promising diagnostic marker, clinical introduction of this marker for cancer diagnosis is
still problematic due to the lack of means of evaluating sample quality. Human telomerase reverse transcriptase (hTERT), one
of the subunits of telomerase, is also a promising diagnostic marker. In the present study, we did large-scale analysis of
88 pancreatic juice samples to determine the feasibility of quantitative analysis of hTERT mRNA for diagnosis of pancreatic
cancer. We found significant differences in hTERT expression among carcinoma-derived, intraductal papillary mucinous neoplasm
(IPMN)–derived, and chronic pancreatitis–derived juice samples. Results showed that quantitative analyses of hTERT mRNAs are
more useful in discriminating carcinoma from IPMN than from chronic pancreatitis. When the specificity was set at 100%, the
sensitivity for differentiation between carcinoma and IPMN was 43.5%, whereas the sensitivity of cytologic examination was
22.0%. There were significant differences in hTERT expression among carcinoma cells, IPMN cells, and normal ductal cells isolated
from pancreatic tissues by microdissection. Lymphocytes and hyperplastic epithelial cells isolated from tissues with the histologic
appearance of pancreatitis showed various expression levels of hTERT. Our results suggest that quantitative analysis of hTERT
mRNA in pancreatic juice is advantageous over cytologic analysis for differentiation between carcinoma and IPMN but probably
not for differentiation between carcinoma and chronic pancreatitis.</description><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>chronic pancreatitis</subject><subject>DNA-Binding Proteins - analysis</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Enzymologic - genetics</subject><subject>Gene Expression Regulation, Neoplastic - genetics</subject><subject>hTERT</subject><subject>Humans</subject><subject>IPMN</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Medical sciences</subject><subject>pancreatic cancer</subject><subject>pancreatic juice</subject><subject>Pancreatic Neoplasms - enzymology</subject><subject>Pancreatic Neoplasms - genetics</subject><subject>Pharmacology. Drug treatments</subject><subject>RNA, Messenger - genetics</subject><subject>Telomerase - analysis</subject><subject>Telomerase - genetics</subject><subject>Tumors</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkElP5DAQRi00iP0ngHIZxCXgSrzliCI2CYlFzdlynMq0R1kaOwHx73HoRhw51afSq0WPkGOg5wBcXQCVKqUsz87L8jmlPAWVwRbZA85lmmeC_4n5m9kl-yH8pxQYULZDdkEIWlCQe2TxNJl-dKMZ3Rsml71pP4ILydAkt1Nn-mSB7dChNwGTZ3xDH-vCmz5Y71bj3HV98mh66zFusEkZI_pDst2YNuDRph6Ql-urRXmb3j_c3JWX96llQo5pIWidVVWBjWBSsKaohZTCWDR5oThXCmhtuECq0GLTKIQaKyoqLjObKSbyA3K63rvyw-uEYdSdCxbb1vQ4TEELqXhesPxXECTkNDqJIF-D1g8heGz0yrvO-A8NVM_e9exUz0519K4p17P3OHeyOTBVHdY_UxvREfi7AUywpm2iQ-vCDydFIZkoIne25pbu3_LdedT2S6nHgMbbpYZMS53FZ_NP8DeZiQ</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>OHUCHIDA, Kenoki</creator><creator>MIZUMOTO, Kazuhiro</creator><creator>YAMADA, Daisuke</creator><creator>YAMAGUCHI, Hiroshi</creator><creator>KONOMI, Hiroyuki</creator><creator>NAGAI, Eishi</creator><creator>YAMAGUCHI, Koji</creator><creator>TSUNEYOSHI, Masazumi</creator><creator>TANAKA, Masao</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Quantitative Analysis of Human Telomerase Reverse Transcriptase in Pancreatic Cancer</title><author>OHUCHIDA, Kenoki ; MIZUMOTO, Kazuhiro ; YAMADA, Daisuke ; YAMAGUCHI, Hiroshi ; KONOMI, Hiroyuki ; NAGAI, Eishi ; YAMAGUCHI, Koji ; TSUNEYOSHI, Masazumi ; TANAKA, Masao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-960d2bb9ef64764f9d6776acea398558810da56e08eceff8e1deb06b572c28463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>chronic pancreatitis</topic><topic>DNA-Binding Proteins - analysis</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Enzymologic - genetics</topic><topic>Gene Expression Regulation, Neoplastic - genetics</topic><topic>hTERT</topic><topic>Humans</topic><topic>IPMN</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Medical sciences</topic><topic>pancreatic cancer</topic><topic>pancreatic juice</topic><topic>Pancreatic Neoplasms - enzymology</topic><topic>Pancreatic Neoplasms - genetics</topic><topic>Pharmacology. Drug treatments</topic><topic>RNA, Messenger - genetics</topic><topic>Telomerase - analysis</topic><topic>Telomerase - genetics</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OHUCHIDA, Kenoki</creatorcontrib><creatorcontrib>MIZUMOTO, Kazuhiro</creatorcontrib><creatorcontrib>YAMADA, Daisuke</creatorcontrib><creatorcontrib>YAMAGUCHI, Hiroshi</creatorcontrib><creatorcontrib>KONOMI, Hiroyuki</creatorcontrib><creatorcontrib>NAGAI, Eishi</creatorcontrib><creatorcontrib>YAMAGUCHI, Koji</creatorcontrib><creatorcontrib>TSUNEYOSHI, Masazumi</creatorcontrib><creatorcontrib>TANAKA, Masao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OHUCHIDA, Kenoki</au><au>MIZUMOTO, Kazuhiro</au><au>YAMADA, Daisuke</au><au>YAMAGUCHI, Hiroshi</au><au>KONOMI, Hiroyuki</au><au>NAGAI, Eishi</au><au>YAMAGUCHI, Koji</au><au>TSUNEYOSHI, Masazumi</au><au>TANAKA, Masao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative Analysis of Human Telomerase Reverse Transcriptase in Pancreatic Cancer</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>12</volume><issue>7</issue><spage>2066</spage><epage>2069</epage><pages>2066-2069</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><abstract>Although telomerase activity is a promising diagnostic marker, clinical introduction of this marker for cancer diagnosis is
still problematic due to the lack of means of evaluating sample quality. Human telomerase reverse transcriptase (hTERT), one
of the subunits of telomerase, is also a promising diagnostic marker. In the present study, we did large-scale analysis of
88 pancreatic juice samples to determine the feasibility of quantitative analysis of hTERT mRNA for diagnosis of pancreatic
cancer. We found significant differences in hTERT expression among carcinoma-derived, intraductal papillary mucinous neoplasm
(IPMN)–derived, and chronic pancreatitis–derived juice samples. Results showed that quantitative analyses of hTERT mRNAs are
more useful in discriminating carcinoma from IPMN than from chronic pancreatitis. When the specificity was set at 100%, the
sensitivity for differentiation between carcinoma and IPMN was 43.5%, whereas the sensitivity of cytologic examination was
22.0%. There were significant differences in hTERT expression among carcinoma cells, IPMN cells, and normal ductal cells isolated
from pancreatic tissues by microdissection. Lymphocytes and hyperplastic epithelial cells isolated from tissues with the histologic
appearance of pancreatitis showed various expression levels of hTERT. Our results suggest that quantitative analysis of hTERT
mRNA in pancreatic juice is advantageous over cytologic analysis for differentiation between carcinoma and IPMN but probably
not for differentiation between carcinoma and chronic pancreatitis.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>16609017</pmid><doi>10.1158/1078-0432.CCR-05-1821</doi><tpages>4</tpages></addata></record> |
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| ispartof | Clinical cancer research, 2006-04, Vol.12 (7), p.2066-2069 |
| issn | 1078-0432 1557-3265 |
| language | eng |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Antineoplastic agents Biological and medical sciences chronic pancreatitis DNA-Binding Proteins - analysis DNA-Binding Proteins - genetics Gastroenterology. Liver. Pancreas. Abdomen Gene Expression Profiling Gene Expression Regulation, Enzymologic - genetics Gene Expression Regulation, Neoplastic - genetics hTERT Humans IPMN Liver. Biliary tract. Portal circulation. Exocrine pancreas Medical sciences pancreatic cancer pancreatic juice Pancreatic Neoplasms - enzymology Pancreatic Neoplasms - genetics Pharmacology. Drug treatments RNA, Messenger - genetics Telomerase - analysis Telomerase - genetics Tumors |
| title | Quantitative Analysis of Human Telomerase Reverse Transcriptase in Pancreatic Cancer |
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