Influence of epidermal growth factor and insulin-like growth factor 1 on nuclear maturation and fertilization of buffalo cumulus oocyte complexes in serum free media and their subsequent development in vitro

The in vitro maturation, fertilization and development of Indian water buffalo ( Bubalus sp.) cumulus oocyte complexes (COCs) to blastocysts were studied during culture, either in serum free tissue culture medium 199 (TCM 199) or Waymouth MB (WM). Based on different supplements added to these media,...

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Veröffentlicht in:	Animal reproduction science 2005-07, Vol.87 (3), p.229-239
Hauptverfasser:	Purohit, G.N., Brady, M.S., Sharma, S.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals blastocyst Blastomeres - cytology Blastomeres - physiology Buffalo buffaloes Buffaloes - embryology Buffaloes - physiology culture media Culture Media, Serum-Free cumulus oophorus EGF, IGF-1 embryo culture Embryo Culture Techniques - methods Embryo Culture Techniques - veterinary embryogenesis epidermal growth factor Epidermal Growth Factor - pharmacology estradiol Estradiol - physiology Female females Fertilization in Vitro - veterinary Follicle Stimulating Hormone - physiology follicle-stimulating hormone Hormones In vitro culture in vitro fertilization insulin-like growth factor I Insulin-Like Growth Factor I - pharmacology Linear Models luteinizing hormone Luteinizing Hormone - physiology Male Media oocytes Oocytes - drug effects Oocytes - growth & development Oocytes - physiology Pregnancy
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