Fluorescence In Situ Hybridization for the Detection of t(X;18)(p11.2;q11.2) in a Synovial Sarcoma Tissue Microarray Using a Breakapart-Style Probe
Synovial sarcomas (SSs) account for 5% of soft tissue tumors and carry a balanced translocation t(X;18)(p11.2;q11.2), detectable in over 90% of cases. This translocation brings together portions of two genesSYT and SSX. Detecting interruption of the SYT gene on chromosome 18 would be useful as a dia...
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| Veröffentlicht in: | Diagnostic molecular pathology 2005-06, Vol.14 (2), p.77-82 |
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| creator | Terry, Jefferson Barry, Todd S Horsman, Douglas E Hsu, Forrest D Gown, Allen M Huntsman, David G Nielsen, Torsten O |
| description | Synovial sarcomas (SSs) account for 5% of soft tissue tumors and carry a balanced translocation t(X;18)(p11.2;q11.2), detectable in over 90% of cases. This translocation brings together portions of two genesSYT and SSX. Detecting interruption of the SYT gene on chromosome 18 would be useful as a diagnostic tool. We describe a scoring method to detect disruption of SYT with breakapart probe fluorescence in situ hybridization (FISH) and the application of this method for identification of SS within a sarcoma tissue microarray. After optimization, SYT disruption was identified in 22 of 23 (96%) of known SS tumor samples but was not in 23 of 23 (100%) of non-SS sarcoma samples. Ten of 11 (91%) blinded test SS tumor samples were also correctly identified. For comparison, commercially available FISH and chromogenic in situ hybridization (CISH) probes were tested. The commercial FISH probes identified SYT disruption in 81% of the SS tumor samples but in none of the non-SS samples. The CISH probes produced signals too weak to interpret. The use of breakapart FISH probes is a relatively quick procedure for detection of synovial sarcoma translocations and can be applied to archival specimens in tissue microarrays. |
| doi_str_mv | 10.1097/01.pas.0000155021.80213.c9 |
| format | Article |
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This translocation brings together portions of two genesSYT and SSX. Detecting interruption of the SYT gene on chromosome 18 would be useful as a diagnostic tool. We describe a scoring method to detect disruption of SYT with breakapart probe fluorescence in situ hybridization (FISH) and the application of this method for identification of SS within a sarcoma tissue microarray. After optimization, SYT disruption was identified in 22 of 23 (96%) of known SS tumor samples but was not in 23 of 23 (100%) of non-SS sarcoma samples. Ten of 11 (91%) blinded test SS tumor samples were also correctly identified. For comparison, commercially available FISH and chromogenic in situ hybridization (CISH) probes were tested. The commercial FISH probes identified SYT disruption in 81% of the SS tumor samples but in none of the non-SS samples. The CISH probes produced signals too weak to interpret. 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This translocation brings together portions of two genesSYT and SSX. Detecting interruption of the SYT gene on chromosome 18 would be useful as a diagnostic tool. We describe a scoring method to detect disruption of SYT with breakapart probe fluorescence in situ hybridization (FISH) and the application of this method for identification of SS within a sarcoma tissue microarray. After optimization, SYT disruption was identified in 22 of 23 (96%) of known SS tumor samples but was not in 23 of 23 (100%) of non-SS sarcoma samples. Ten of 11 (91%) blinded test SS tumor samples were also correctly identified. For comparison, commercially available FISH and chromogenic in situ hybridization (CISH) probes were tested. The commercial FISH probes identified SYT disruption in 81% of the SS tumor samples but in none of the non-SS samples. The CISH probes produced signals too weak to interpret. The use of breakapart FISH probes is a relatively quick procedure for detection of synovial sarcoma translocations and can be applied to archival specimens in tissue microarrays.</description><subject>Chromosomes, Human, Pair 11 - genetics</subject><subject>Chromosomes, Human, Pair 18 - genetics</subject><subject>DNA, Complementary - chemistry</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Nucleic Acid Probes</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Repressor Proteins - genetics</subject><subject>Sarcoma, Synovial - diagnosis</subject><subject>Sarcoma, Synovial - genetics</subject><subject>Sequence Deletion</subject><subject>Soft Tissue Neoplasms - diagnosis</subject><subject>Soft Tissue Neoplasms - genetics</subject><subject>Tissue Array Analysis</subject><subject>Translocation, Genetic</subject><issn>1052-9551</issn><issn>1533-4066</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1u1DAQgCMEoqXwCsjigNpDgieOvQ49lUJppSKQtpW4WRPHYU2zcWo7VOE1eGGc7kr1Yfyjb2Y0_rLsHdACaL36QKEYMRQ0LeCcllDIFFih62fZIXDG8ooK8TydKS_zmnM4yF6F8DvxrJL8ZXYAvKZc1PQw-3fRT86boM2gDbkayNrGiVzOjbet_YvRuoF0zpO4MeSziUY_vriOxOOfpyBPjkeAojy9X-IJsQNBsp4H98diT9botdsiubEhTIZ8s9o79B5nchvs8Cuhn7zBOxzRx3wd596QH9415nX2osM-mDf7_Si7vfhyc36ZX3__enV-dp1rJsoqZwabUiJoITToUneYpipRgOCyAdaVnLeVXEktKSs1iFZLXcnGcNFhW1WaHWXvd3VH7-4nE6La2vQRfY-DcVNQYiU51LJO4McdmAYIwZtOjd5u0c8KqFqUKAoqKVFPStSjEqWX5Lf7LlOzNe1T6t5BAqod8OD6aHy466cH49XGYB83S0moBRV5SSmnIl3zpUvF_gM_WpeI</recordid><startdate>200506</startdate><enddate>200506</enddate><creator>Terry, Jefferson</creator><creator>Barry, Todd S</creator><creator>Horsman, Douglas E</creator><creator>Hsu, Forrest D</creator><creator>Gown, Allen M</creator><creator>Huntsman, David G</creator><creator>Nielsen, Torsten O</creator><general>Lippincott Williams & Wilkins, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200506</creationdate><title>Fluorescence In Situ Hybridization for the Detection of t(X;18)(p11.2;q11.2) in a Synovial Sarcoma Tissue Microarray Using a Breakapart-Style Probe</title><author>Terry, Jefferson ; Barry, Todd S ; Horsman, Douglas E ; Hsu, Forrest D ; Gown, Allen M ; Huntsman, David G ; Nielsen, Torsten O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3624-3eab28a1c66c1c2cfa5902a61658b13f255d4878c8032c16dc8c48be56fad44c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Chromosomes, Human, Pair 11 - genetics</topic><topic>Chromosomes, Human, Pair 18 - genetics</topic><topic>DNA, Complementary - chemistry</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Nucleic Acid Probes</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Repressor Proteins - genetics</topic><topic>Sarcoma, Synovial - diagnosis</topic><topic>Sarcoma, Synovial - genetics</topic><topic>Sequence Deletion</topic><topic>Soft Tissue Neoplasms - diagnosis</topic><topic>Soft Tissue Neoplasms - genetics</topic><topic>Tissue Array Analysis</topic><topic>Translocation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Terry, Jefferson</creatorcontrib><creatorcontrib>Barry, Todd S</creatorcontrib><creatorcontrib>Horsman, Douglas E</creatorcontrib><creatorcontrib>Hsu, Forrest D</creatorcontrib><creatorcontrib>Gown, Allen M</creatorcontrib><creatorcontrib>Huntsman, David G</creatorcontrib><creatorcontrib>Nielsen, Torsten O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic molecular pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Terry, Jefferson</au><au>Barry, Todd S</au><au>Horsman, Douglas E</au><au>Hsu, Forrest D</au><au>Gown, Allen M</au><au>Huntsman, David G</au><au>Nielsen, Torsten O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescence In Situ Hybridization for the Detection of t(X;18)(p11.2;q11.2) in a Synovial Sarcoma Tissue Microarray Using a Breakapart-Style Probe</atitle><jtitle>Diagnostic molecular pathology</jtitle><addtitle>Diagn Mol Pathol</addtitle><date>2005-06</date><risdate>2005</risdate><volume>14</volume><issue>2</issue><spage>77</spage><epage>82</epage><pages>77-82</pages><issn>1052-9551</issn><eissn>1533-4066</eissn><abstract>Synovial sarcomas (SSs) account for 5% of soft tissue tumors and carry a balanced translocation t(X;18)(p11.2;q11.2), detectable in over 90% of cases. This translocation brings together portions of two genesSYT and SSX. Detecting interruption of the SYT gene on chromosome 18 would be useful as a diagnostic tool. We describe a scoring method to detect disruption of SYT with breakapart probe fluorescence in situ hybridization (FISH) and the application of this method for identification of SS within a sarcoma tissue microarray. After optimization, SYT disruption was identified in 22 of 23 (96%) of known SS tumor samples but was not in 23 of 23 (100%) of non-SS sarcoma samples. Ten of 11 (91%) blinded test SS tumor samples were also correctly identified. For comparison, commercially available FISH and chromogenic in situ hybridization (CISH) probes were tested. The commercial FISH probes identified SYT disruption in 81% of the SS tumor samples but in none of the non-SS samples. The CISH probes produced signals too weak to interpret. 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| ispartof | Diagnostic molecular pathology, 2005-06, Vol.14 (2), p.77-82 |
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| subjects | Chromosomes, Human, Pair 11 - genetics Chromosomes, Human, Pair 18 - genetics DNA, Complementary - chemistry Humans In Situ Hybridization, Fluorescence - methods Nucleic Acid Probes Proto-Oncogene Proteins - genetics Repressor Proteins - genetics Sarcoma, Synovial - diagnosis Sarcoma, Synovial - genetics Sequence Deletion Soft Tissue Neoplasms - diagnosis Soft Tissue Neoplasms - genetics Tissue Array Analysis Translocation, Genetic |
| title | Fluorescence In Situ Hybridization for the Detection of t(X;18)(p11.2;q11.2) in a Synovial Sarcoma Tissue Microarray Using a Breakapart-Style Probe |
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