A rapid isocratic high-performance liquid chromatography method for determination of cholesterol and 1,2-dioleoyl- sn-glycero-3-phosphocholine in liposome-based drug formulations
A high-performance liquid chromatography (HPLC) method for the determination of cholesterol and 1,2-dioleoyl- sn-glycero-3-phosphocholine (DOPC) in liposome-based drug formulations has been developed. Liposome formulations of anticancer agents (viz., paclitaxel, docetaxel, 7-ethyl-10-hydroxycamptoth...
Gespeichert in:
| Veröffentlicht in: | Journal of Chromatography A 2005-05, Vol.1073 (1), p.347-353 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 353 |
|---|---|
| container_issue | 1 |
| container_start_page | 347 |
| container_title | Journal of Chromatography A |
| container_volume | 1073 |
| creator | Singh, Ramsharan Ajagbe, Monsurat Bhamidipati, Shastri Ahmad, Zafeer Ahmad, Imran |
| description | A high-performance liquid chromatography (HPLC) method for the determination of cholesterol and 1,2-dioleoyl-
sn-glycero-3-phosphocholine (DOPC) in liposome-based drug formulations has been developed. Liposome formulations of anticancer agents (viz., paclitaxel, docetaxel, 7-ethyl-10-hydroxycamptothecin (SN38), doxorubicin, mitoxantrone and an antisense oligodeoxyribonucleotide, etc.) were prepared. These formulations contain DOPC, cholesterol and other lipids, such as tetramyristoyl cardiolipin or 1,3-bis(1,2-bis-tetradecyloxy-propyl-3-dimethylethoxyammonium bromide)propan-2-ol [(
R)-PCL-2] in product-specific ratios. A simple HPLC method that uses isocratic elution and UV detection has been developed for simultaneous quantification of cholesterol and DOPC components of the liposome formulations. The chromatographic separation of these components is achieved using a C
8 analytical column with 50
mM ammonium phosphate buffer (pH 2.7)–methanol (15:85, v/v) as mobile phase. Both cholesterol and DOPC peaks are well resolved and free of interference from other excipients or degraded impurities in the formulation. The method has been found to be linear (
r
>
0.999) over a wide concentration range of both analytes. This method offers the advantage of simultaneous quantitation of cholesterol and DOPC in various liposome-based formulations without any preprocessing of the sample, and has quantitation limits of 0.5 and 10
μg/mL for cholesterol and DOPC, respectively. |
| doi_str_mv | 10.1016/j.chroma.2004.12.036 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67851244</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021967304023040</els_id><sourcerecordid>67851244</sourcerecordid><originalsourceid>FETCH-LOGICAL-c390t-df5a927634387597fc237a905f97f8355772d4a58c015d0761a1311979fbdb473</originalsourceid><addsrcrecordid>eNp9kctu1DAUhr0A0VL6Bgh5A6s6-BLHyQapqoBWqsSGri2P7Uw8cuLUTpDmtXhCzjQjdcfCsnX8_ef2I_SR0YpR1nw9VHbIaTQVp7SuGK-oaN6gS0o5I12jxAV6X8qBUqao4u_QBZMd7WRNL9HfW5zNHBwOJdlslmDxEPYDmX3uUx7NZD2O4XkFYiuxpD0IhiMe_TIkh4HCzi8-j2ECeZpw6gFN0RcIpojN5DC74cQFiKVjJLhMZB-PFn6JIPOQCpyTIkwehwnKzamk0ZOdKd5hl9f9qcq4xpf85QN625tY_PX5vkJPP77_vrsnj79-PtzdPhIrOroQ10vTcdWIWrRKdqq3XCjTUdnDuxVSKsVdbWRrKZOOqoYZJhjrVNfv3K5W4gp92fLOOT2vMI4eQ7E-RjP5tBbdqFYyXtcA1htocyol-17POYwmHzWj-uSPPuhtefrkj2Zcgz8g-3TOv-5G715FZ3MA-HwGTLEm9hncCOWVgwZaKiVw3zbOwzb-BJ91scGDcy5kbxftUvh_J_8Anwy1tA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67851244</pqid></control><display><type>article</type><title>A rapid isocratic high-performance liquid chromatography method for determination of cholesterol and 1,2-dioleoyl- sn-glycero-3-phosphocholine in liposome-based drug formulations</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Singh, Ramsharan ; Ajagbe, Monsurat ; Bhamidipati, Shastri ; Ahmad, Zafeer ; Ahmad, Imran</creator><creatorcontrib>Singh, Ramsharan ; Ajagbe, Monsurat ; Bhamidipati, Shastri ; Ahmad, Zafeer ; Ahmad, Imran</creatorcontrib><description>A high-performance liquid chromatography (HPLC) method for the determination of cholesterol and 1,2-dioleoyl-
sn-glycero-3-phosphocholine (DOPC) in liposome-based drug formulations has been developed. Liposome formulations of anticancer agents (viz., paclitaxel, docetaxel, 7-ethyl-10-hydroxycamptothecin (SN38), doxorubicin, mitoxantrone and an antisense oligodeoxyribonucleotide, etc.) were prepared. These formulations contain DOPC, cholesterol and other lipids, such as tetramyristoyl cardiolipin or 1,3-bis(1,2-bis-tetradecyloxy-propyl-3-dimethylethoxyammonium bromide)propan-2-ol [(
R)-PCL-2] in product-specific ratios. A simple HPLC method that uses isocratic elution and UV detection has been developed for simultaneous quantification of cholesterol and DOPC components of the liposome formulations. The chromatographic separation of these components is achieved using a C
8 analytical column with 50
mM ammonium phosphate buffer (pH 2.7)–methanol (15:85, v/v) as mobile phase. Both cholesterol and DOPC peaks are well resolved and free of interference from other excipients or degraded impurities in the formulation. The method has been found to be linear (
r
>
0.999) over a wide concentration range of both analytes. This method offers the advantage of simultaneous quantitation of cholesterol and DOPC in various liposome-based formulations without any preprocessing of the sample, and has quantitation limits of 0.5 and 10
μg/mL for cholesterol and DOPC, respectively.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2004.12.036</identifier><identifier>PMID: 15909540</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>1,2-Dioleoyl- sn-glycero-3-phosphocholine ; Analysis ; Anticancer drugs ; Antioxidants ; Biological and medical sciences ; Cholesterol ; Cholesterol - analysis ; Chromatography, High Pressure Liquid - methods ; General pharmacology ; Lipids ; Liposomes ; Medical sciences ; Pharmaceutical Preparations - chemistry ; Pharmacology. Drug treatments ; Phosphatidylcholines - analysis ; Phospholipids ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrophotometry, Ultraviolet ; Tocopherols</subject><ispartof>Journal of Chromatography A, 2005-05, Vol.1073 (1), p.347-353</ispartof><rights>2004</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-df5a927634387597fc237a905f97f8355772d4a58c015d0761a1311979fbdb473</citedby><cites>FETCH-LOGICAL-c390t-df5a927634387597fc237a905f97f8355772d4a58c015d0761a1311979fbdb473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chroma.2004.12.036$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16788055$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15909540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, Ramsharan</creatorcontrib><creatorcontrib>Ajagbe, Monsurat</creatorcontrib><creatorcontrib>Bhamidipati, Shastri</creatorcontrib><creatorcontrib>Ahmad, Zafeer</creatorcontrib><creatorcontrib>Ahmad, Imran</creatorcontrib><title>A rapid isocratic high-performance liquid chromatography method for determination of cholesterol and 1,2-dioleoyl- sn-glycero-3-phosphocholine in liposome-based drug formulations</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>A high-performance liquid chromatography (HPLC) method for the determination of cholesterol and 1,2-dioleoyl-
sn-glycero-3-phosphocholine (DOPC) in liposome-based drug formulations has been developed. Liposome formulations of anticancer agents (viz., paclitaxel, docetaxel, 7-ethyl-10-hydroxycamptothecin (SN38), doxorubicin, mitoxantrone and an antisense oligodeoxyribonucleotide, etc.) were prepared. These formulations contain DOPC, cholesterol and other lipids, such as tetramyristoyl cardiolipin or 1,3-bis(1,2-bis-tetradecyloxy-propyl-3-dimethylethoxyammonium bromide)propan-2-ol [(
R)-PCL-2] in product-specific ratios. A simple HPLC method that uses isocratic elution and UV detection has been developed for simultaneous quantification of cholesterol and DOPC components of the liposome formulations. The chromatographic separation of these components is achieved using a C
8 analytical column with 50
mM ammonium phosphate buffer (pH 2.7)–methanol (15:85, v/v) as mobile phase. Both cholesterol and DOPC peaks are well resolved and free of interference from other excipients or degraded impurities in the formulation. The method has been found to be linear (
r
>
0.999) over a wide concentration range of both analytes. This method offers the advantage of simultaneous quantitation of cholesterol and DOPC in various liposome-based formulations without any preprocessing of the sample, and has quantitation limits of 0.5 and 10
μg/mL for cholesterol and DOPC, respectively.</description><subject>1,2-Dioleoyl- sn-glycero-3-phosphocholine</subject><subject>Analysis</subject><subject>Anticancer drugs</subject><subject>Antioxidants</subject><subject>Biological and medical sciences</subject><subject>Cholesterol</subject><subject>Cholesterol - analysis</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>General pharmacology</subject><subject>Lipids</subject><subject>Liposomes</subject><subject>Medical sciences</subject><subject>Pharmaceutical Preparations - chemistry</subject><subject>Pharmacology. Drug treatments</subject><subject>Phosphatidylcholines - analysis</subject><subject>Phospholipids</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Tocopherols</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhr0A0VL6Bgh5A6s6-BLHyQapqoBWqsSGri2P7Uw8cuLUTpDmtXhCzjQjdcfCsnX8_ef2I_SR0YpR1nw9VHbIaTQVp7SuGK-oaN6gS0o5I12jxAV6X8qBUqao4u_QBZMd7WRNL9HfW5zNHBwOJdlslmDxEPYDmX3uUx7NZD2O4XkFYiuxpD0IhiMe_TIkh4HCzi8-j2ECeZpw6gFN0RcIpojN5DC74cQFiKVjJLhMZB-PFn6JIPOQCpyTIkwehwnKzamk0ZOdKd5hl9f9qcq4xpf85QN625tY_PX5vkJPP77_vrsnj79-PtzdPhIrOroQ10vTcdWIWrRKdqq3XCjTUdnDuxVSKsVdbWRrKZOOqoYZJhjrVNfv3K5W4gp92fLOOT2vMI4eQ7E-RjP5tBbdqFYyXtcA1htocyol-17POYwmHzWj-uSPPuhtefrkj2Zcgz8g-3TOv-5G715FZ3MA-HwGTLEm9hncCOWVgwZaKiVw3zbOwzb-BJ91scGDcy5kbxftUvh_J_8Anwy1tA</recordid><startdate>20050506</startdate><enddate>20050506</enddate><creator>Singh, Ramsharan</creator><creator>Ajagbe, Monsurat</creator><creator>Bhamidipati, Shastri</creator><creator>Ahmad, Zafeer</creator><creator>Ahmad, Imran</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050506</creationdate><title>A rapid isocratic high-performance liquid chromatography method for determination of cholesterol and 1,2-dioleoyl- sn-glycero-3-phosphocholine in liposome-based drug formulations</title><author>Singh, Ramsharan ; Ajagbe, Monsurat ; Bhamidipati, Shastri ; Ahmad, Zafeer ; Ahmad, Imran</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-df5a927634387597fc237a905f97f8355772d4a58c015d0761a1311979fbdb473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>1,2-Dioleoyl- sn-glycero-3-phosphocholine</topic><topic>Analysis</topic><topic>Anticancer drugs</topic><topic>Antioxidants</topic><topic>Biological and medical sciences</topic><topic>Cholesterol</topic><topic>Cholesterol - analysis</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>General pharmacology</topic><topic>Lipids</topic><topic>Liposomes</topic><topic>Medical sciences</topic><topic>Pharmaceutical Preparations - chemistry</topic><topic>Pharmacology. Drug treatments</topic><topic>Phosphatidylcholines - analysis</topic><topic>Phospholipids</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Tocopherols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Ramsharan</creatorcontrib><creatorcontrib>Ajagbe, Monsurat</creatorcontrib><creatorcontrib>Bhamidipati, Shastri</creatorcontrib><creatorcontrib>Ahmad, Zafeer</creatorcontrib><creatorcontrib>Ahmad, Imran</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Ramsharan</au><au>Ajagbe, Monsurat</au><au>Bhamidipati, Shastri</au><au>Ahmad, Zafeer</au><au>Ahmad, Imran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid isocratic high-performance liquid chromatography method for determination of cholesterol and 1,2-dioleoyl- sn-glycero-3-phosphocholine in liposome-based drug formulations</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2005-05-06</date><risdate>2005</risdate><volume>1073</volume><issue>1</issue><spage>347</spage><epage>353</epage><pages>347-353</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A high-performance liquid chromatography (HPLC) method for the determination of cholesterol and 1,2-dioleoyl-
sn-glycero-3-phosphocholine (DOPC) in liposome-based drug formulations has been developed. Liposome formulations of anticancer agents (viz., paclitaxel, docetaxel, 7-ethyl-10-hydroxycamptothecin (SN38), doxorubicin, mitoxantrone and an antisense oligodeoxyribonucleotide, etc.) were prepared. These formulations contain DOPC, cholesterol and other lipids, such as tetramyristoyl cardiolipin or 1,3-bis(1,2-bis-tetradecyloxy-propyl-3-dimethylethoxyammonium bromide)propan-2-ol [(
R)-PCL-2] in product-specific ratios. A simple HPLC method that uses isocratic elution and UV detection has been developed for simultaneous quantification of cholesterol and DOPC components of the liposome formulations. The chromatographic separation of these components is achieved using a C
8 analytical column with 50
mM ammonium phosphate buffer (pH 2.7)–methanol (15:85, v/v) as mobile phase. Both cholesterol and DOPC peaks are well resolved and free of interference from other excipients or degraded impurities in the formulation. The method has been found to be linear (
r
>
0.999) over a wide concentration range of both analytes. This method offers the advantage of simultaneous quantitation of cholesterol and DOPC in various liposome-based formulations without any preprocessing of the sample, and has quantitation limits of 0.5 and 10
μg/mL for cholesterol and DOPC, respectively.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15909540</pmid><doi>10.1016/j.chroma.2004.12.036</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9673 |
| ispartof | Journal of Chromatography A, 2005-05, Vol.1073 (1), p.347-353 |
| issn | 0021-9673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67851244 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 1,2-Dioleoyl- sn-glycero-3-phosphocholine Analysis Anticancer drugs Antioxidants Biological and medical sciences Cholesterol Cholesterol - analysis Chromatography, High Pressure Liquid - methods General pharmacology Lipids Liposomes Medical sciences Pharmaceutical Preparations - chemistry Pharmacology. Drug treatments Phosphatidylcholines - analysis Phospholipids Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet Tocopherols |
| title | A rapid isocratic high-performance liquid chromatography method for determination of cholesterol and 1,2-dioleoyl- sn-glycero-3-phosphocholine in liposome-based drug formulations |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T10%3A13%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20rapid%20isocratic%20high-performance%20liquid%20chromatography%20method%20for%20determination%20of%20cholesterol%20and%201,2-dioleoyl-%20sn-glycero-3-phosphocholine%20in%20liposome-based%20drug%20formulations&rft.jtitle=Journal%20of%20Chromatography%20A&rft.au=Singh,%20Ramsharan&rft.date=2005-05-06&rft.volume=1073&rft.issue=1&rft.spage=347&rft.epage=353&rft.pages=347-353&rft.issn=0021-9673&rft.coden=JOCRAM&rft_id=info:doi/10.1016/j.chroma.2004.12.036&rft_dat=%3Cproquest_cross%3E67851244%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67851244&rft_id=info:pmid/15909540&rft_els_id=S0021967304023040&rfr_iscdi=true |