Movement of potexviruses requires species-specific interactions among the cognate triple gene block proteins, as revealed by a trans-complementation assay based on the bamboo mosaic virus satellite RNA-mediated expression system

The intra- and intercellular transport of potexviruses require interactions among viral RNA, coat protein and elements of the triple gene block proteins (TGBps). In this study, the requirement of bamboo mosaic virus (BaMV) TGBps for movement functions and the compatibilities with those of two potexv...

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Veröffentlicht in:Journal of general virology 2006-05, Vol.87 (5), p.1357-1367
Hauptverfasser: Lin, M.K, Hu, C.C, Lin, N.S, Chang, B.Y, Hsu, Y.H
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container_title Journal of general virology
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creator Lin, M.K
Hu, C.C
Lin, N.S
Chang, B.Y
Hsu, Y.H
description The intra- and intercellular transport of potexviruses require interactions among viral RNA, coat protein and elements of the triple gene block proteins (TGBps). In this study, the requirement of bamboo mosaic virus (BaMV) TGBps for movement functions and the compatibilities with those of two potexviruses, Potato virus X (PVX) and Foxtail mosaic virus (FoMV), were examined using a satellite RNA-mediated trans-complementation assay system. Single or multiple TGBps of BaMV, PVX and FoMV were expressed from BaMV satellite RNA (satBaMV RNA) vectors to complement the functions of green fluorescent protein-tagged, movement-defective BaMV with mutation(s) in the matching gene(s). It was found that individual BaMV TGBps expressed from the satellite vector could function normally in trans, whereas bi-gene BaMV TGBp constructs in which the expression of TGBp3 might be impaired and individual TGBp genes from PVX or FoMV could not complement the movement functions of the defective helper viruses. Furthermore, alterations of the ratio among TGBps by ectopic expression of individual components of TGBps from satBaMV RNA vectors did not affect the cell-to-cell movement capabilities of wild-type BaMV significantly. The results indicate that species-specific interactions among movement proteins are obligatory for the cell-to-cell movement of BaMV and possibly other potexviruses.
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In this study, the requirement of bamboo mosaic virus (BaMV) TGBps for movement functions and the compatibilities with those of two potexviruses, Potato virus X (PVX) and Foxtail mosaic virus (FoMV), were examined using a satellite RNA-mediated trans-complementation assay system. Single or multiple TGBps of BaMV, PVX and FoMV were expressed from BaMV satellite RNA (satBaMV RNA) vectors to complement the functions of green fluorescent protein-tagged, movement-defective BaMV with mutation(s) in the matching gene(s). It was found that individual BaMV TGBps expressed from the satellite vector could function normally in trans, whereas bi-gene BaMV TGBp constructs in which the expression of TGBp3 might be impaired and individual TGBp genes from PVX or FoMV could not complement the movement functions of the defective helper viruses. Furthermore, alterations of the ratio among TGBps by ectopic expression of individual components of TGBps from satBaMV RNA vectors did not affect the cell-to-cell movement capabilities of wild-type BaMV significantly. The results indicate that species-specific interactions among movement proteins are obligatory for the cell-to-cell movement of BaMV and possibly other potexviruses.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.81625-0</identifier><identifier>PMID: 16603539</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Bamboo mosaic virus ; Biological and medical sciences ; cell to cell movement ; Chenopodium quinoa ; Foxtail mosaic virus ; Fundamental and applied biological sciences. 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In this study, the requirement of bamboo mosaic virus (BaMV) TGBps for movement functions and the compatibilities with those of two potexviruses, Potato virus X (PVX) and Foxtail mosaic virus (FoMV), were examined using a satellite RNA-mediated trans-complementation assay system. Single or multiple TGBps of BaMV, PVX and FoMV were expressed from BaMV satellite RNA (satBaMV RNA) vectors to complement the functions of green fluorescent protein-tagged, movement-defective BaMV with mutation(s) in the matching gene(s). It was found that individual BaMV TGBps expressed from the satellite vector could function normally in trans, whereas bi-gene BaMV TGBp constructs in which the expression of TGBp3 might be impaired and individual TGBp genes from PVX or FoMV could not complement the movement functions of the defective helper viruses. 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Psychology</topic><topic>genetic complementation</topic><topic>Genetic Techniques</topic><topic>genetic vectors</topic><topic>host-pathogen relationships</topic><topic>intercellular movement</topic><topic>Locomotion</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>movement proteins</topic><topic>mutants</topic><topic>Mutation</topic><topic>Nicotiana benthamiana</topic><topic>pathogenesis</topic><topic>Plants - virology</topic><topic>plasmodesmata</topic><topic>Potato virus X</topic><topic>Potexvirus - genetics</topic><topic>Potexvirus - metabolism</topic><topic>RNA, Satellite</topic><topic>RNA, Viral - genetics</topic><topic>satellite RNA</topic><topic>Species Specificity</topic><topic>triple gene block proteins</topic><topic>viral proteins</topic><topic>Viral Proteins - metabolism</topic><topic>Viral Proteins - physiology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, M.K</creatorcontrib><creatorcontrib>Hu, C.C</creatorcontrib><creatorcontrib>Lin, N.S</creatorcontrib><creatorcontrib>Chang, B.Y</creatorcontrib><creatorcontrib>Hsu, Y.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, M.K</au><au>Hu, C.C</au><au>Lin, N.S</au><au>Chang, B.Y</au><au>Hsu, Y.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Movement of potexviruses requires species-specific interactions among the cognate triple gene block proteins, as revealed by a trans-complementation assay based on the bamboo mosaic virus satellite RNA-mediated expression system</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>87</volume><issue>5</issue><spage>1357</spage><epage>1367</epage><pages>1357-1367</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>The intra- and intercellular transport of potexviruses require interactions among viral RNA, coat protein and elements of the triple gene block proteins (TGBps). In this study, the requirement of bamboo mosaic virus (BaMV) TGBps for movement functions and the compatibilities with those of two potexviruses, Potato virus X (PVX) and Foxtail mosaic virus (FoMV), were examined using a satellite RNA-mediated trans-complementation assay system. Single or multiple TGBps of BaMV, PVX and FoMV were expressed from BaMV satellite RNA (satBaMV RNA) vectors to complement the functions of green fluorescent protein-tagged, movement-defective BaMV with mutation(s) in the matching gene(s). It was found that individual BaMV TGBps expressed from the satellite vector could function normally in trans, whereas bi-gene BaMV TGBp constructs in which the expression of TGBp3 might be impaired and individual TGBp genes from PVX or FoMV could not complement the movement functions of the defective helper viruses. Furthermore, alterations of the ratio among TGBps by ectopic expression of individual components of TGBps from satBaMV RNA vectors did not affect the cell-to-cell movement capabilities of wild-type BaMV significantly. The results indicate that species-specific interactions among movement proteins are obligatory for the cell-to-cell movement of BaMV and possibly other potexviruses.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>16603539</pmid><doi>10.1099/vir.0.81625-0</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Bamboo mosaic virus
Biological and medical sciences
cell to cell movement
Chenopodium quinoa
Foxtail mosaic virus
Fundamental and applied biological sciences. Psychology
genetic complementation
Genetic Techniques
genetic vectors
host-pathogen relationships
intercellular movement
Locomotion
Microbiology
Miscellaneous
movement proteins
mutants
Mutation
Nicotiana benthamiana
pathogenesis
Plants - virology
plasmodesmata
Potato virus X
Potexvirus - genetics
Potexvirus - metabolism
RNA, Satellite
RNA, Viral - genetics
satellite RNA
Species Specificity
triple gene block proteins
viral proteins
Viral Proteins - metabolism
Viral Proteins - physiology
Virology
title Movement of potexviruses requires species-specific interactions among the cognate triple gene block proteins, as revealed by a trans-complementation assay based on the bamboo mosaic virus satellite RNA-mediated expression system
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