Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa
[Display omitted] An arabinogalactan was isolated from a hot water extract of freeze-dried cells of the green microalga, Chlorella pyrenoidosa. This hot water extract is a proprietary immunomodulator, with the trademark Respondin™ (ONC-107). The arabinogalactan was recovered from the ethanol-soluble...
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| Veröffentlicht in: | Carbohydrate research 2005-06, Vol.340 (8), p.1489-1498 |
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| container_title | Carbohydrate research |
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| creator | Suárez, Erick Reyes Kralovec, Jaroslav A. Noseda, Miguel D. Ewart, H. Stephen Barrow, Colin J. Lumsden, Michael D. Grindley, T. Bruce |
| description | [Display omitted]
An arabinogalactan was isolated from a hot water extract of freeze-dried cells of the green microalga,
Chlorella pyrenoidosa. This hot water extract is a proprietary immunomodulator, with the trademark Respondin™ (ONC-107). The arabinogalactan was recovered from the ethanol-soluble fraction of the supernatant resulting from a process that involved controlled ethanol precipitation followed by size exclusion chromatography on Sephadex G-100, then Cetavlon precipitation. Sugar analyses, GC–MS data for (
S)-2-octyl glycosides, and 1D and 2D NMR experiments established unambiguously that the repeating unit was →2)-α-
l-Ara
f-(1→3)-[α-
l-Ara
f-(1→4)]-β-
d-Gal
p-(1→. This structure does not fit into any of the known classes of arabinogalactans. SEC/MALS experiments gave a molecular mass for the arabinogalactan isolated as 47
±
4
kDa but the original structure was probably larger. |
| doi_str_mv | 10.1016/j.carres.2005.04.003 |
| format | Article |
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An arabinogalactan was isolated from a hot water extract of freeze-dried cells of the green microalga,
Chlorella pyrenoidosa. This hot water extract is a proprietary immunomodulator, with the trademark Respondin™ (ONC-107). The arabinogalactan was recovered from the ethanol-soluble fraction of the supernatant resulting from a process that involved controlled ethanol precipitation followed by size exclusion chromatography on Sephadex G-100, then Cetavlon precipitation. Sugar analyses, GC–MS data for (
S)-2-octyl glycosides, and 1D and 2D NMR experiments established unambiguously that the repeating unit was →2)-α-
l-Ara
f-(1→3)-[α-
l-Ara
f-(1→4)]-β-
d-Gal
p-(1→. This structure does not fit into any of the known classes of arabinogalactans. SEC/MALS experiments gave a molecular mass for the arabinogalactan isolated as 47
±
4
kDa but the original structure was probably larger.</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>DOI: 10.1016/j.carres.2005.04.003</identifier><identifier>PMID: 15882847</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Arabinogalactans ; Carbohydrate Sequence ; Cell Extracts - chemistry ; Cell Extracts - immunology ; Cell Line ; Chlorella - chemistry ; Chlorella - immunology ; Chlorella pyrenoidosa ; Chromatography, Gel ; Dextrans ; Galactans - chemistry ; Galactans - isolation & purification ; Immunomodulators ; Magnetic Resonance Spectroscopy ; Mice ; Molecular Sequence Data ; Molecular Weight ; Nitric Oxide - metabolism ; NMR spectroscopy ; Polysaccharide structure determination</subject><ispartof>Carbohydrate research, 2005-06, Vol.340 (8), p.1489-1498</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-6458abba4e74cb4a52e3a0c4768beb1859bc1808c25072f4d113b225ff1eea4f3</citedby><cites>FETCH-LOGICAL-c360t-6458abba4e74cb4a52e3a0c4768beb1859bc1808c25072f4d113b225ff1eea4f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008621505001655$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15882847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suárez, Erick Reyes</creatorcontrib><creatorcontrib>Kralovec, Jaroslav A.</creatorcontrib><creatorcontrib>Noseda, Miguel D.</creatorcontrib><creatorcontrib>Ewart, H. Stephen</creatorcontrib><creatorcontrib>Barrow, Colin J.</creatorcontrib><creatorcontrib>Lumsden, Michael D.</creatorcontrib><creatorcontrib>Grindley, T. Bruce</creatorcontrib><title>Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>[Display omitted]
An arabinogalactan was isolated from a hot water extract of freeze-dried cells of the green microalga,
Chlorella pyrenoidosa. This hot water extract is a proprietary immunomodulator, with the trademark Respondin™ (ONC-107). The arabinogalactan was recovered from the ethanol-soluble fraction of the supernatant resulting from a process that involved controlled ethanol precipitation followed by size exclusion chromatography on Sephadex G-100, then Cetavlon precipitation. Sugar analyses, GC–MS data for (
S)-2-octyl glycosides, and 1D and 2D NMR experiments established unambiguously that the repeating unit was →2)-α-
l-Ara
f-(1→3)-[α-
l-Ara
f-(1→4)]-β-
d-Gal
p-(1→. This structure does not fit into any of the known classes of arabinogalactans. SEC/MALS experiments gave a molecular mass for the arabinogalactan isolated as 47
±
4
kDa but the original structure was probably larger.</description><subject>Animals</subject><subject>Arabinogalactans</subject><subject>Carbohydrate Sequence</subject><subject>Cell Extracts - chemistry</subject><subject>Cell Extracts - immunology</subject><subject>Cell Line</subject><subject>Chlorella - chemistry</subject><subject>Chlorella - immunology</subject><subject>Chlorella pyrenoidosa</subject><subject>Chromatography, Gel</subject><subject>Dextrans</subject><subject>Galactans - chemistry</subject><subject>Galactans - isolation & purification</subject><subject>Immunomodulators</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Nitric Oxide - metabolism</subject><subject>NMR spectroscopy</subject><subject>Polysaccharide structure determination</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uc1u1DAQthCIbgtvgJBPnEiwHcdxL0hoxU-lSlxA4mZNnAn1KrEX26m6vAivi9OsxI3TaGa-nxl9hLzirOaMq3eH2kKMmGrBWFszWTPWPCE7rrumkkL9eEp2jDFdKcHbC3KZ0qG0THXqObngrdZCy25H_tykMEF2wb-l9g4i2IzR_X6cUPADTTkuNi8RJjpg2c3Ob8swUqCLd78WpPl0xMdBhN758BOmogOejjHMRYW6eV58SNnNSzEL8UTxIa9eK2l_N4WI0wT0eIrogxtCghfk2QhTwpfnekW-f_r4bf-luv36-Wb_4bayjWK5UrLV0PcgsZO2l9AKbIBZ2SndY891e91brpm2omWdGOXAedML0Y4jRwQ5NlfkzaZ7jKF8krKZXbLrNR7DkozqtFTXqilAuQFtDClFHM0xuhniyXBm1kDMwWyBmDUQw6QpgRTa67P-0s84_COdEyiA9xsAy5f3DqNJ1qG3OLiINpshuP87_AXVtKOq</recordid><startdate>20050613</startdate><enddate>20050613</enddate><creator>Suárez, Erick Reyes</creator><creator>Kralovec, Jaroslav A.</creator><creator>Noseda, Miguel D.</creator><creator>Ewart, H. Stephen</creator><creator>Barrow, Colin J.</creator><creator>Lumsden, Michael D.</creator><creator>Grindley, T. Bruce</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050613</creationdate><title>Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa</title><author>Suárez, Erick Reyes ; Kralovec, Jaroslav A. ; Noseda, Miguel D. ; Ewart, H. Stephen ; Barrow, Colin J. ; Lumsden, Michael D. ; Grindley, T. Bruce</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-6458abba4e74cb4a52e3a0c4768beb1859bc1808c25072f4d113b225ff1eea4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Arabinogalactans</topic><topic>Carbohydrate Sequence</topic><topic>Cell Extracts - chemistry</topic><topic>Cell Extracts - immunology</topic><topic>Cell Line</topic><topic>Chlorella - chemistry</topic><topic>Chlorella - immunology</topic><topic>Chlorella pyrenoidosa</topic><topic>Chromatography, Gel</topic><topic>Dextrans</topic><topic>Galactans - chemistry</topic><topic>Galactans - isolation & purification</topic><topic>Immunomodulators</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Nitric Oxide - metabolism</topic><topic>NMR spectroscopy</topic><topic>Polysaccharide structure determination</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suárez, Erick Reyes</creatorcontrib><creatorcontrib>Kralovec, Jaroslav A.</creatorcontrib><creatorcontrib>Noseda, Miguel D.</creatorcontrib><creatorcontrib>Ewart, H. Stephen</creatorcontrib><creatorcontrib>Barrow, Colin J.</creatorcontrib><creatorcontrib>Lumsden, Michael D.</creatorcontrib><creatorcontrib>Grindley, T. Bruce</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suárez, Erick Reyes</au><au>Kralovec, Jaroslav A.</au><au>Noseda, Miguel D.</au><au>Ewart, H. Stephen</au><au>Barrow, Colin J.</au><au>Lumsden, Michael D.</au><au>Grindley, T. Bruce</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>2005-06-13</date><risdate>2005</risdate><volume>340</volume><issue>8</issue><spage>1489</spage><epage>1498</epage><pages>1489-1498</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><abstract>[Display omitted]
An arabinogalactan was isolated from a hot water extract of freeze-dried cells of the green microalga,
Chlorella pyrenoidosa. This hot water extract is a proprietary immunomodulator, with the trademark Respondin™ (ONC-107). The arabinogalactan was recovered from the ethanol-soluble fraction of the supernatant resulting from a process that involved controlled ethanol precipitation followed by size exclusion chromatography on Sephadex G-100, then Cetavlon precipitation. Sugar analyses, GC–MS data for (
S)-2-octyl glycosides, and 1D and 2D NMR experiments established unambiguously that the repeating unit was →2)-α-
l-Ara
f-(1→3)-[α-
l-Ara
f-(1→4)]-β-
d-Gal
p-(1→. This structure does not fit into any of the known classes of arabinogalactans. SEC/MALS experiments gave a molecular mass for the arabinogalactan isolated as 47
±
4
kDa but the original structure was probably larger.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>15882847</pmid><doi>10.1016/j.carres.2005.04.003</doi><tpages>10</tpages></addata></record> |
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| ispartof | Carbohydrate research, 2005-06, Vol.340 (8), p.1489-1498 |
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| subjects | Animals Arabinogalactans Carbohydrate Sequence Cell Extracts - chemistry Cell Extracts - immunology Cell Line Chlorella - chemistry Chlorella - immunology Chlorella pyrenoidosa Chromatography, Gel Dextrans Galactans - chemistry Galactans - isolation & purification Immunomodulators Magnetic Resonance Spectroscopy Mice Molecular Sequence Data Molecular Weight Nitric Oxide - metabolism NMR spectroscopy Polysaccharide structure determination |
| title | Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa |
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