Determination and assay validation of pinosylvin in rat serum: application to drug metabolism and pharmacokinetics
A method of analysis of pinosylvin in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism and determine its concentration in natural products. A novel and simple high-performance liquid chromatographic method was developed for simultaneous determination of pinosyl...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2005-06, Vol.38 (1), p.148-154 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 154 |
|---|---|
| container_issue | 1 |
| container_start_page | 148 |
| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 38 |
| creator | Roupe, Kathryn Halls, Steven Davies, Neal M. |
| description | A method of analysis of pinosylvin in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism and determine its concentration in natural products. A novel and simple high-performance liquid chromatographic method was developed for simultaneous determination of pinosylvin and products of its metabolism in rat serum and liver microsomes. Serum, or microsomes (0.1
mL) were precipitated with acetonitrile after addition of the internal standard, 7-ethoxycoumarin. Separation was achieved on an amylose tris 3,5 dimethylphenylcarbamate column (150
mm
×
4.6
mm, ID, 5
μm) with UV detection at 308
nm. The calibration curves were linear ranging from 0.5 to 100
μg/mL. The mean extraction efficiency was >99%. Precision of the assay (coefficient of variation) was |
| doi_str_mv | 10.1016/j.jpba.2004.12.015 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67846486</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708504006880</els_id><sourcerecordid>67846486</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-3a5ab74ce7feec1f0cf6224f430074c4608d15ada822f252c1be8b17b0ce98e83</originalsourceid><addsrcrecordid>eNp9kEGL1TAQx4Mo7tvVL-BBetFb6yRN0zzxIqurwoIXBW9hmk40z7apSfrgfXv77IO9CQMDw2_-M_wYe8Gh4sDVm0N1mDusBICsuKiAN4_Yjuu2LoWSPx6zHbQ1L1vQzRW7TukAAA3fy6fsijd7aFVd71j8QJni6CfMPkwFTn2BKeGpOOLg-20YXDH7KaTTcPRTsVbEXCSKy_i2wHkevN24HIo-Lj-LkTJ2YfBp_Jc3_8I4og2__UTZ2_SMPXE4JHp-6Tfs-93Hb7efy_uvn77cvr8vba1lLmtssGulpdYRWe7AOiWEdLIGWMdSge55gz1qIZxohOUd6Y63HVjaa9L1DXu95c4x_FkoZTP6ZGkYcKKwJKNaLZXUagXFBtoYUorkzBz9iPFkOJizaXMwZ9PmbNpwYVbT69LLS_rSjdQ_rFzUrsCrC4DJ4uAiTtanB05pAL0_X3-3cbS6OHqKJllPk6XeR7LZ9MH_74-_tZ2fHw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67846486</pqid></control><display><type>article</type><title>Determination and assay validation of pinosylvin in rat serum: application to drug metabolism and pharmacokinetics</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Roupe, Kathryn ; Halls, Steven ; Davies, Neal M.</creator><creatorcontrib>Roupe, Kathryn ; Halls, Steven ; Davies, Neal M.</creatorcontrib><description>A method of analysis of pinosylvin in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism and determine its concentration in natural products. A novel and simple high-performance liquid chromatographic method was developed for simultaneous determination of pinosylvin and products of its metabolism in rat serum and liver microsomes. Serum, or microsomes (0.1
mL) were precipitated with acetonitrile after addition of the internal standard, 7-ethoxycoumarin. Separation was achieved on an amylose tris 3,5 dimethylphenylcarbamate column (150
mm
×
4.6
mm, ID, 5
μm) with UV detection at 308
nm. The calibration curves were linear ranging from 0.5 to 100
μg/mL. The mean extraction efficiency was >99%. Precision of the assay (coefficient of variation) was <10%, including the limit of quantitation (0.5
μg/mL). Bias of the assay was lower than 15%. The limit of detection was 100
ng/mL for a 0.1
mL sample. The assay was successfully applied to both the in vitro and in vivo metabolic kinetic study of pinosylvin. Three metabolites of pinosylvin, two oxidative and one glucuronidated, have been identified. The two oxidative metabolites of pinosylvin have been identified as E- and Z
-resveratrol.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2004.12.015</identifier><identifier>PMID: 15907633</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Kinetics ; Male ; Medical sciences ; Microsomes, Liver - metabolism ; Pharmacology. Drug treatments ; Pinosylvin ; Rats ; Rats, Sprague-Dawley ; Reference Standards ; Reproducibility of Results ; Reversed-phase HPLC ; Sensitivity and Specificity ; Spectrometry, Mass, Electrospray Ionization - methods ; Spectrophotometry, Ultraviolet ; Stilbenes - blood ; Stilbenes - pharmacokinetics ; UV-detection</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2005-06, Vol.38 (1), p.148-154</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-3a5ab74ce7feec1f0cf6224f430074c4608d15ada822f252c1be8b17b0ce98e83</citedby><cites>FETCH-LOGICAL-c384t-3a5ab74ce7feec1f0cf6224f430074c4608d15ada822f252c1be8b17b0ce98e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2004.12.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16800896$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15907633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roupe, Kathryn</creatorcontrib><creatorcontrib>Halls, Steven</creatorcontrib><creatorcontrib>Davies, Neal M.</creatorcontrib><title>Determination and assay validation of pinosylvin in rat serum: application to drug metabolism and pharmacokinetics</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A method of analysis of pinosylvin in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism and determine its concentration in natural products. A novel and simple high-performance liquid chromatographic method was developed for simultaneous determination of pinosylvin and products of its metabolism in rat serum and liver microsomes. Serum, or microsomes (0.1
mL) were precipitated with acetonitrile after addition of the internal standard, 7-ethoxycoumarin. Separation was achieved on an amylose tris 3,5 dimethylphenylcarbamate column (150
mm
×
4.6
mm, ID, 5
μm) with UV detection at 308
nm. The calibration curves were linear ranging from 0.5 to 100
μg/mL. The mean extraction efficiency was >99%. Precision of the assay (coefficient of variation) was <10%, including the limit of quantitation (0.5
μg/mL). Bias of the assay was lower than 15%. The limit of detection was 100
ng/mL for a 0.1
mL sample. The assay was successfully applied to both the in vitro and in vivo metabolic kinetic study of pinosylvin. Three metabolites of pinosylvin, two oxidative and one glucuronidated, have been identified. The two oxidative metabolites of pinosylvin have been identified as E- and Z
-resveratrol.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Kinetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microsomes, Liver - metabolism</subject><subject>Pharmacology. Drug treatments</subject><subject>Pinosylvin</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Reversed-phase HPLC</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Stilbenes - blood</subject><subject>Stilbenes - pharmacokinetics</subject><subject>UV-detection</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEGL1TAQx4Mo7tvVL-BBetFb6yRN0zzxIqurwoIXBW9hmk40z7apSfrgfXv77IO9CQMDw2_-M_wYe8Gh4sDVm0N1mDusBICsuKiAN4_Yjuu2LoWSPx6zHbQ1L1vQzRW7TukAAA3fy6fsijd7aFVd71j8QJni6CfMPkwFTn2BKeGpOOLg-20YXDH7KaTTcPRTsVbEXCSKy_i2wHkevN24HIo-Lj-LkTJ2YfBp_Jc3_8I4og2__UTZ2_SMPXE4JHp-6Tfs-93Hb7efy_uvn77cvr8vba1lLmtssGulpdYRWe7AOiWEdLIGWMdSge55gz1qIZxohOUd6Y63HVjaa9L1DXu95c4x_FkoZTP6ZGkYcKKwJKNaLZXUagXFBtoYUorkzBz9iPFkOJizaXMwZ9PmbNpwYVbT69LLS_rSjdQ_rFzUrsCrC4DJ4uAiTtanB05pAL0_X3-3cbS6OHqKJllPk6XeR7LZ9MH_74-_tZ2fHw</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Roupe, Kathryn</creator><creator>Halls, Steven</creator><creator>Davies, Neal M.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Determination and assay validation of pinosylvin in rat serum: application to drug metabolism and pharmacokinetics</title><author>Roupe, Kathryn ; Halls, Steven ; Davies, Neal M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-3a5ab74ce7feec1f0cf6224f430074c4608d15ada822f252c1be8b17b0ce98e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Kinetics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microsomes, Liver - metabolism</topic><topic>Pharmacology. Drug treatments</topic><topic>Pinosylvin</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Reversed-phase HPLC</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Stilbenes - blood</topic><topic>Stilbenes - pharmacokinetics</topic><topic>UV-detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roupe, Kathryn</creatorcontrib><creatorcontrib>Halls, Steven</creatorcontrib><creatorcontrib>Davies, Neal M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roupe, Kathryn</au><au>Halls, Steven</au><au>Davies, Neal M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination and assay validation of pinosylvin in rat serum: application to drug metabolism and pharmacokinetics</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>38</volume><issue>1</issue><spage>148</spage><epage>154</epage><pages>148-154</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A method of analysis of pinosylvin in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism and determine its concentration in natural products. A novel and simple high-performance liquid chromatographic method was developed for simultaneous determination of pinosylvin and products of its metabolism in rat serum and liver microsomes. Serum, or microsomes (0.1
mL) were precipitated with acetonitrile after addition of the internal standard, 7-ethoxycoumarin. Separation was achieved on an amylose tris 3,5 dimethylphenylcarbamate column (150
mm
×
4.6
mm, ID, 5
μm) with UV detection at 308
nm. The calibration curves were linear ranging from 0.5 to 100
μg/mL. The mean extraction efficiency was >99%. Precision of the assay (coefficient of variation) was <10%, including the limit of quantitation (0.5
μg/mL). Bias of the assay was lower than 15%. The limit of detection was 100
ng/mL for a 0.1
mL sample. The assay was successfully applied to both the in vitro and in vivo metabolic kinetic study of pinosylvin. Three metabolites of pinosylvin, two oxidative and one glucuronidated, have been identified. The two oxidative metabolites of pinosylvin have been identified as E- and Z
-resveratrol.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15907633</pmid><doi>10.1016/j.jpba.2004.12.015</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2005-06, Vol.38 (1), p.148-154 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67846486 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid - methods Fundamental and applied biological sciences. Psychology General pharmacology Kinetics Male Medical sciences Microsomes, Liver - metabolism Pharmacology. Drug treatments Pinosylvin Rats Rats, Sprague-Dawley Reference Standards Reproducibility of Results Reversed-phase HPLC Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods Spectrophotometry, Ultraviolet Stilbenes - blood Stilbenes - pharmacokinetics UV-detection |
| title | Determination and assay validation of pinosylvin in rat serum: application to drug metabolism and pharmacokinetics |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T18%3A07%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20and%20assay%20validation%20of%20pinosylvin%20in%20rat%20serum:%20application%20to%20drug%20metabolism%20and%20pharmacokinetics&rft.jtitle=Journal%20of%20pharmaceutical%20and%20biomedical%20analysis&rft.au=Roupe,%20Kathryn&rft.date=2005-06-01&rft.volume=38&rft.issue=1&rft.spage=148&rft.epage=154&rft.pages=148-154&rft.issn=0731-7085&rft.eissn=1873-264X&rft.coden=JPBADA&rft_id=info:doi/10.1016/j.jpba.2004.12.015&rft_dat=%3Cproquest_cross%3E67846486%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67846486&rft_id=info:pmid/15907633&rft_els_id=S0731708504006880&rfr_iscdi=true |