Separation of thiamin and its phosphate esters by capillary zone electrophoresis and its application to the analysis of water-soluble vitamins
A capillary zone electrophoresis method for the separation and determination of thiamin and its phosphate esters (free thiamin, thiamin monophosphate, and thiamin pyrophosphate) was developed and optimized. The efficiency achieved with boric acid–sodium tetraborate decahydrate buffer (pH 8.24; 65–8...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2005-06, Vol.38 (1), p.66-71 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Shabangi, Masangu Sutton, Jeffrey A. |
| description | A capillary zone electrophoresis method for the separation and determination of thiamin and its phosphate esters (free thiamin, thiamin monophosphate, and thiamin pyrophosphate) was developed and optimized. The efficiency achieved with boric acid–sodium tetraborate decahydrate buffer (pH 8.24; 65–8
mM) at an applied potential of 30
kV gave the detection limit (S/N
=
3) and the limit of quantitative measurement (S/N
=
10) of thiamin and its phosphate esters ranging from 10
−4 to 6
×
10
−4
mM and from 6
×
10
−4 to 1.2
×
10
−3
mM, respectively. The effects of pH on separation and migration times of thiamin and its phosphate esters are described. The method was validated and applied to the quantitative determination of thiamin in commercial tablets containing both a massive and a normal dose of thiamin. |
| doi_str_mv | 10.1016/j.jpba.2004.11.061 |
| format | Article |
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mM) at an applied potential of 30
kV gave the detection limit (S/N
=
3) and the limit of quantitative measurement (S/N
=
10) of thiamin and its phosphate esters ranging from 10
−4 to 6
×
10
−4
mM and from 6
×
10
−4 to 1.2
×
10
−3
mM, respectively. The effects of pH on separation and migration times of thiamin and its phosphate esters are described. The method was validated and applied to the quantitative determination of thiamin in commercial tablets containing both a massive and a normal dose of thiamin.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2004.11.061</identifier><identifier>PMID: 15907621</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Capillary zone electrophoresis ; Electrophoresis, Capillary - methods ; Esters ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Hydrogen-Ion Concentration ; Medical sciences ; Pharmacology. Drug treatments ; Phosphates ; Sensitivity and Specificity ; Separation ; Solubility ; Thiamin esters ; Thiamine - chemistry ; Thiamine - isolation & purification ; Vitamins - analysis ; Water</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2005-06, Vol.38 (1), p.66-71</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-6d7258454c9b465f392b59a3258b01cfb8095c6dec630562d25e0362f76f87543</citedby><cites>FETCH-LOGICAL-c384t-6d7258454c9b465f392b59a3258b01cfb8095c6dec630562d25e0362f76f87543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2004.11.061$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16800884$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15907621$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shabangi, Masangu</creatorcontrib><creatorcontrib>Sutton, Jeffrey A.</creatorcontrib><title>Separation of thiamin and its phosphate esters by capillary zone electrophoresis and its application to the analysis of water-soluble vitamins</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A capillary zone electrophoresis method for the separation and determination of thiamin and its phosphate esters (free thiamin, thiamin monophosphate, and thiamin pyrophosphate) was developed and optimized. The efficiency achieved with boric acid–sodium tetraborate decahydrate buffer (pH 8.24; 65–8
mM) at an applied potential of 30
kV gave the detection limit (S/N
=
3) and the limit of quantitative measurement (S/N
=
10) of thiamin and its phosphate esters ranging from 10
−4 to 6
×
10
−4
mM and from 6
×
10
−4 to 1.2
×
10
−3
mM, respectively. The effects of pH on separation and migration times of thiamin and its phosphate esters are described. The method was validated and applied to the quantitative determination of thiamin in commercial tablets containing both a massive and a normal dose of thiamin.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Capillary zone electrophoresis</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Esters</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Phosphates</subject><subject>Sensitivity and Specificity</subject><subject>Separation</subject><subject>Solubility</subject><subject>Thiamin esters</subject><subject>Thiamine - chemistry</subject><subject>Thiamine - isolation & purification</subject><subject>Vitamins - analysis</subject><subject>Water</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9q3DAQxkVpaLZpX6CHokt7szOSLVmGXkroPwj00BZ6E7I8ZrVoLVfSJmweIs9cmV2aW08CzW--mW8-Qt4wqBkweb2rd8tgag7Q1ozVINkzsmGqayou29_PyQa6hlUdKHFJXqa0AwDB-vYFuWSih05ytiGPP3Ax0WQXZhommrfO7N1MzTxSlxNdtiEtW5ORYsoYEx2O1JrFeW_ikT6EuRQ82hxDISMml_61mmXxzp6UcyjKWErGH1emTLovorFKwR8Gj_TO5XVuekUuJuMTvj6_V-TX508_b75Wt9-_fLv5eFvZRrW5kmPHhWpFa_uhlWJqej6I3jTlcwBmp0FBL6wc0coGhOQjFwiN5FMnJ9WJtrki70-6Swx_DsWb3rtksdiaMRySll1R5yAKyE-gjSGliJNeotsX85qBXlPQO72moNcUNGO6pFCa3p7VD8Mex6eW89kL8O4MmGSNn6KZrUtPnFQASq1rfjhxWG5x5zDqZB3OFkcXy9X1GNz_9vgL1lOoHA</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Shabangi, Masangu</creator><creator>Sutton, Jeffrey A.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Separation of thiamin and its phosphate esters by capillary zone electrophoresis and its application to the analysis of water-soluble vitamins</title><author>Shabangi, Masangu ; Sutton, Jeffrey A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-6d7258454c9b465f392b59a3258b01cfb8095c6dec630562d25e0362f76f87543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Capillary zone electrophoresis</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Esters</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Phosphates</topic><topic>Sensitivity and Specificity</topic><topic>Separation</topic><topic>Solubility</topic><topic>Thiamin esters</topic><topic>Thiamine - chemistry</topic><topic>Thiamine - isolation & purification</topic><topic>Vitamins - analysis</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shabangi, Masangu</creatorcontrib><creatorcontrib>Sutton, Jeffrey A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shabangi, Masangu</au><au>Sutton, Jeffrey A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of thiamin and its phosphate esters by capillary zone electrophoresis and its application to the analysis of water-soluble vitamins</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>38</volume><issue>1</issue><spage>66</spage><epage>71</epage><pages>66-71</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A capillary zone electrophoresis method for the separation and determination of thiamin and its phosphate esters (free thiamin, thiamin monophosphate, and thiamin pyrophosphate) was developed and optimized. The efficiency achieved with boric acid–sodium tetraborate decahydrate buffer (pH 8.24; 65–8
mM) at an applied potential of 30
kV gave the detection limit (S/N
=
3) and the limit of quantitative measurement (S/N
=
10) of thiamin and its phosphate esters ranging from 10
−4 to 6
×
10
−4
mM and from 6
×
10
−4 to 1.2
×
10
−3
mM, respectively. The effects of pH on separation and migration times of thiamin and its phosphate esters are described. The method was validated and applied to the quantitative determination of thiamin in commercial tablets containing both a massive and a normal dose of thiamin.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15907621</pmid><doi>10.1016/j.jpba.2004.11.061</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2005-06, Vol.38 (1), p.66-71 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67845205 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Capillary zone electrophoresis Electrophoresis, Capillary - methods Esters Fundamental and applied biological sciences. Psychology General pharmacology Hydrogen-Ion Concentration Medical sciences Pharmacology. Drug treatments Phosphates Sensitivity and Specificity Separation Solubility Thiamin esters Thiamine - chemistry Thiamine - isolation & purification Vitamins - analysis Water |
| title | Separation of thiamin and its phosphate esters by capillary zone electrophoresis and its application to the analysis of water-soluble vitamins |
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