Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain
Objectives: High-level penicillin resistance in Streptococcus pneumoniae requires extensive re-modulation of the penicillin-binding proteins (PBPs), and murM gene function is also required for the expression of resistance. In this work, we determined whether specific changes in PBPs were associated...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2006-05, Vol.57 (5), p.983-986 |
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| creator | del Campo, Rosa Cafini, Fabio Morosini, María Isabel Fenoll, Asunción Liñares, Josefina Alou, Luis Sevillano, David Cantón, Rafael Prieto, José Baquero, Fernando |
| description | Objectives: High-level penicillin resistance in Streptococcus pneumoniae requires extensive re-modulation of the penicillin-binding proteins (PBPs), and murM gene function is also required for the expression of resistance. In this work, we determined whether specific changes in PBPs were associated with specific MurM variants. Methods: Two collections of highly penicillin-resistant (MIC 2–8 mg/L) isolates, including 10 early (1997–1998) and 23 contemporary (2002–2004) isolates, were studied. Results: Most of the isolates belonged to clones Spain6B-2 (13 strains), Spain23F-1 (10 isolates) and Spain14-5 (20 isolates). Different protein variants of MurM (MA, MB5, MB6, MB9 and MB10), PBP1A (A–C), PBP2B (A–D) and PBP2X (A–C) were recognized, including two murM alleles not previously described. Particular [MurM-PBP1A-2B-2X] allelic combinations were predominant among the different clones, including [MA-B-B-B] for old (MIC 2 mg/L) and [MB10-C-A-B] for recent (MIC 4–8 mg/L) Spain6B-2 isolates, [MA-A-C-A] for Spain23F-1 and [MB5-A-A-A] in Spain14-5 isolates. Conclusions: Although S. pneumoniae has a basic recombinational population structure, our results indicate remarkable conservation of PBPs and MurM protein types within each clone. This suggests that particular PBPs–MurM combinations tend to be preserved and may have an independent evolutionary history in particular clones. |
| doi_str_mv | 10.1093/jac/dkl083 |
| format | Article |
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In this work, we determined whether specific changes in PBPs were associated with specific MurM variants. Methods: Two collections of highly penicillin-resistant (MIC 2–8 mg/L) isolates, including 10 early (1997–1998) and 23 contemporary (2002–2004) isolates, were studied. Results: Most of the isolates belonged to clones Spain6B-2 (13 strains), Spain23F-1 (10 isolates) and Spain14-5 (20 isolates). Different protein variants of MurM (MA, MB5, MB6, MB9 and MB10), PBP1A (A–C), PBP2B (A–D) and PBP2X (A–C) were recognized, including two murM alleles not previously described. Particular [MurM-PBP1A-2B-2X] allelic combinations were predominant among the different clones, including [MA-B-B-B] for old (MIC 2 mg/L) and [MB10-C-A-B] for recent (MIC 4–8 mg/L) Spain6B-2 isolates, [MA-A-C-A] for Spain23F-1 and [MB5-A-A-A] in Spain14-5 isolates. Conclusions: Although S. pneumoniae has a basic recombinational population structure, our results indicate remarkable conservation of PBPs and MurM protein types within each clone. This suggests that particular PBPs–MurM combinations tend to be preserved and may have an independent evolutionary history in particular clones.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkl083</identifier><identifier>PMID: 16533824</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Alleles ; Anti-Bacterial Agents - pharmacology ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Bacterial Proteins - genetics ; Biological and medical sciences ; clones ; Genetic Variation ; Humans ; Medical sciences ; Microbial Sensitivity Tests ; mutations ; Penicillin Resistance - genetics ; penicillin-binding proteins ; Penicillin-Binding Proteins - genetics ; Penicillins - pharmacology ; Peptide Synthases - genetics ; Pharmacology. Drug treatments ; resistance ; Serotyping ; Spain ; Species Specificity ; Streptococcus pneumoniae ; Streptococcus pneumoniae - genetics ; Streptococcus pneumoniae - isolation & purification</subject><ispartof>Journal of antimicrobial chemotherapy, 2006-05, Vol.57 (5), p.983-986</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) May 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-ab0fad1c26ffff84dec84653573050be5e84c2ae4fdccad962bcb1b649efb1a53</citedby><cites>FETCH-LOGICAL-c447t-ab0fad1c26ffff84dec84653573050be5e84c2ae4fdccad962bcb1b649efb1a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17768447$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16533824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>del Campo, Rosa</creatorcontrib><creatorcontrib>Cafini, Fabio</creatorcontrib><creatorcontrib>Morosini, María Isabel</creatorcontrib><creatorcontrib>Fenoll, Asunción</creatorcontrib><creatorcontrib>Liñares, Josefina</creatorcontrib><creatorcontrib>Alou, Luis</creatorcontrib><creatorcontrib>Sevillano, David</creatorcontrib><creatorcontrib>Cantón, Rafael</creatorcontrib><creatorcontrib>Prieto, José</creatorcontrib><creatorcontrib>Baquero, Fernando</creatorcontrib><creatorcontrib>Spanish Pneumococcal Network (G3/103)</creatorcontrib><title>Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J. Antimicrob. Chemother</addtitle><description>Objectives: High-level penicillin resistance in Streptococcus pneumoniae requires extensive re-modulation of the penicillin-binding proteins (PBPs), and murM gene function is also required for the expression of resistance. In this work, we determined whether specific changes in PBPs were associated with specific MurM variants. Methods: Two collections of highly penicillin-resistant (MIC 2–8 mg/L) isolates, including 10 early (1997–1998) and 23 contemporary (2002–2004) isolates, were studied. Results: Most of the isolates belonged to clones Spain6B-2 (13 strains), Spain23F-1 (10 isolates) and Spain14-5 (20 isolates). Different protein variants of MurM (MA, MB5, MB6, MB9 and MB10), PBP1A (A–C), PBP2B (A–D) and PBP2X (A–C) were recognized, including two murM alleles not previously described. Particular [MurM-PBP1A-2B-2X] allelic combinations were predominant among the different clones, including [MA-B-B-B] for old (MIC 2 mg/L) and [MB10-C-A-B] for recent (MIC 4–8 mg/L) Spain6B-2 isolates, [MA-A-C-A] for Spain23F-1 and [MB5-A-A-A] in Spain14-5 isolates. Conclusions: Although S. pneumoniae has a basic recombinational population structure, our results indicate remarkable conservation of PBPs and MurM protein types within each clone. This suggests that particular PBPs–MurM combinations tend to be preserved and may have an independent evolutionary history in particular clones.</description><subject>Alleles</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>clones</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>mutations</subject><subject>Penicillin Resistance - genetics</subject><subject>penicillin-binding proteins</subject><subject>Penicillin-Binding Proteins - genetics</subject><subject>Penicillins - pharmacology</subject><subject>Peptide Synthases - genetics</subject><subject>Pharmacology. Drug treatments</subject><subject>resistance</subject><subject>Serotyping</subject><subject>Spain</subject><subject>Species Specificity</subject><subject>Streptococcus pneumoniae</subject><subject>Streptococcus pneumoniae - genetics</subject><subject>Streptococcus pneumoniae - isolation & purification</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFr1TAYhoso7mx64w-QILgLoS5p06S9nAfdkW062IThTfiafnU5S5OatMP9Dv-wOTsHB96YmwTy5P148mbZK0bfM9qUR2vQR92tpXX5JFswLmhe0IY9zRa0pFUueVXuZfsxrimlohL182yPiaos64Ivst9LP7TGwWS8i8T35OLDRSTgOnI-h3MyBj-hceQOggE3RZLOCMHePyDauwmH0QcI9-TG_LjJLd6hJSM6o421xuUBo4lTekoup4Dj5LXXeo5kdDgP3hlAYqK3MOFD9uUIxr3InvVgI77c7QfZt08fr5ar_Ozryefl8VmuOZdTDi3toWO6EH1aNe9Q1zyJVTJp0xYrrLkuAHnfaQ1dI4pWt6wVvMG-ZVCVB9nhNjdZ_pwxTmowUaO14NDPUQlZc84K-V-QScaaoigT-OYfcO3n4JKEKpgUUgqxSXu3hXTwMQbs1RjMkL5QMao2hapUqNoWmuDXu8S5HbB7RHcNJuDtDoCowfYBnDbxkUszk8Vmar7lUh346-89hNvkWcpKra6_q-svJ6erq1OmRPkHv1280w</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>del Campo, Rosa</creator><creator>Cafini, Fabio</creator><creator>Morosini, María Isabel</creator><creator>Fenoll, Asunción</creator><creator>Liñares, Josefina</creator><creator>Alou, Luis</creator><creator>Sevillano, David</creator><creator>Cantón, Rafael</creator><creator>Prieto, José</creator><creator>Baquero, Fernando</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain</title><author>del Campo, Rosa ; Cafini, Fabio ; Morosini, María Isabel ; Fenoll, Asunción ; Liñares, Josefina ; Alou, Luis ; Sevillano, David ; Cantón, Rafael ; Prieto, José ; Baquero, Fernando</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-ab0fad1c26ffff84dec84653573050be5e84c2ae4fdccad962bcb1b649efb1a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Alleles</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Bacterial Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>clones</topic><topic>Genetic Variation</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>mutations</topic><topic>Penicillin Resistance - genetics</topic><topic>penicillin-binding proteins</topic><topic>Penicillin-Binding Proteins - genetics</topic><topic>Penicillins - pharmacology</topic><topic>Peptide Synthases - genetics</topic><topic>Pharmacology. Drug treatments</topic><topic>resistance</topic><topic>Serotyping</topic><topic>Spain</topic><topic>Species Specificity</topic><topic>Streptococcus pneumoniae</topic><topic>Streptococcus pneumoniae - genetics</topic><topic>Streptococcus pneumoniae - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>del Campo, Rosa</creatorcontrib><creatorcontrib>Cafini, Fabio</creatorcontrib><creatorcontrib>Morosini, María Isabel</creatorcontrib><creatorcontrib>Fenoll, Asunción</creatorcontrib><creatorcontrib>Liñares, Josefina</creatorcontrib><creatorcontrib>Alou, Luis</creatorcontrib><creatorcontrib>Sevillano, David</creatorcontrib><creatorcontrib>Cantón, Rafael</creatorcontrib><creatorcontrib>Prieto, José</creatorcontrib><creatorcontrib>Baquero, Fernando</creatorcontrib><creatorcontrib>Spanish Pneumococcal Network (G3/103)</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>del Campo, Rosa</au><au>Cafini, Fabio</au><au>Morosini, María Isabel</au><au>Fenoll, Asunción</au><au>Liñares, Josefina</au><au>Alou, Luis</au><au>Sevillano, David</au><au>Cantón, Rafael</au><au>Prieto, José</au><au>Baquero, Fernando</au><aucorp>Spanish Pneumococcal Network (G3/103)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J. Antimicrob. Chemother</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>57</volume><issue>5</issue><spage>983</spage><epage>986</epage><pages>983-986</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Objectives: High-level penicillin resistance in Streptococcus pneumoniae requires extensive re-modulation of the penicillin-binding proteins (PBPs), and murM gene function is also required for the expression of resistance. In this work, we determined whether specific changes in PBPs were associated with specific MurM variants. Methods: Two collections of highly penicillin-resistant (MIC 2–8 mg/L) isolates, including 10 early (1997–1998) and 23 contemporary (2002–2004) isolates, were studied. Results: Most of the isolates belonged to clones Spain6B-2 (13 strains), Spain23F-1 (10 isolates) and Spain14-5 (20 isolates). Different protein variants of MurM (MA, MB5, MB6, MB9 and MB10), PBP1A (A–C), PBP2B (A–D) and PBP2X (A–C) were recognized, including two murM alleles not previously described. Particular [MurM-PBP1A-2B-2X] allelic combinations were predominant among the different clones, including [MA-B-B-B] for old (MIC 2 mg/L) and [MB10-C-A-B] for recent (MIC 4–8 mg/L) Spain6B-2 isolates, [MA-A-C-A] for Spain23F-1 and [MB5-A-A-A] in Spain14-5 isolates. Conclusions: Although S. pneumoniae has a basic recombinational population structure, our results indicate remarkable conservation of PBPs and MurM protein types within each clone. This suggests that particular PBPs–MurM combinations tend to be preserved and may have an independent evolutionary history in particular clones.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>16533824</pmid><doi>10.1093/jac/dkl083</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alleles Anti-Bacterial Agents - pharmacology Antibiotics. Antiinfectious agents. Antiparasitic agents Bacterial Proteins - genetics Biological and medical sciences clones Genetic Variation Humans Medical sciences Microbial Sensitivity Tests mutations Penicillin Resistance - genetics penicillin-binding proteins Penicillin-Binding Proteins - genetics Penicillins - pharmacology Peptide Synthases - genetics Pharmacology. Drug treatments resistance Serotyping Spain Species Specificity Streptococcus pneumoniae Streptococcus pneumoniae - genetics Streptococcus pneumoniae - isolation & purification |
| title | Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain |
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