Aminopeptidase N Gene Expression and Abundance in Caprine Mammary Gland is Influenced by Circulating Plasma Peptide
This study examined the localization and the effect of circulating peptides on the expression of aminopeptidase N (EC 3.4.11.2) in caprine mammary gland. Four lactating goats in mid to late lactation were used in a crossover design and were subjected to 2 dietary treatments. Abomasal infusion of cas...
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| Veröffentlicht in: | Journal of dairy science 2005-06, Vol.88 (6), p.2055-2064 |
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| creator | Mabjeesh, S. J Gal-Garber, O Milgram, J Feuermann, Y Cohen-Zinder, M Shamay, A |
| description | This study examined the localization and the effect of circulating peptides on the expression of aminopeptidase N (EC 3.4.11.2) in caprine mammary gland. Four lactating goats in mid to late lactation were used in a crossover design and were subjected to 2 dietary treatments. Abomasal infusion of casein hydrolysate was used to increase the concentration of peptide-bound amino acid in the circulation. Samples of mammary gland tissue from each goat were taken by biopsy at the end of each treatment period to measure gene and protein expression of aminopeptidase N in the tissue. There were no measurable effects on feed intake and milk production for any of the treatments. Western blot analysis showed that aminopeptidase N is located on the basolateral side of parenchymal cells and not on the apical membranes. Abomasal infusion of casein hydrolysate caused a marked change in the profile of arterial blood free amino acids and peptide-bound amino acids smaller than 1500Da. Abundance of aminopeptidase N mRNA and protein increased by 51 and 58%, respectively, in casein hydrolysate-infused goats compared with the control treatment. It was concluded that aminopeptidase N is one candidate actively involved in the mammary gland to support protein synthesis and milk production. In accordance with the nutritional conditions in the current experiment, it is suggested that aminopeptidase N expression is partly controlled by the metabolic requirements of the gland and postabsorptive forms of amino acids in the circulation. |
| doi_str_mv | 10.3168/jds.S0022-0302(05)72882-4 |
| format | Article |
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J ; Gal-Garber, O ; Milgram, J ; Feuermann, Y ; Cohen-Zinder, M ; Shamay, A</creator><creatorcontrib>Mabjeesh, S. J ; Gal-Garber, O ; Milgram, J ; Feuermann, Y ; Cohen-Zinder, M ; Shamay, A</creatorcontrib><description>This study examined the localization and the effect of circulating peptides on the expression of aminopeptidase N (EC 3.4.11.2) in caprine mammary gland. Four lactating goats in mid to late lactation were used in a crossover design and were subjected to 2 dietary treatments. Abomasal infusion of casein hydrolysate was used to increase the concentration of peptide-bound amino acid in the circulation. Samples of mammary gland tissue from each goat were taken by biopsy at the end of each treatment period to measure gene and protein expression of aminopeptidase N in the tissue. There were no measurable effects on feed intake and milk production for any of the treatments. Western blot analysis showed that aminopeptidase N is located on the basolateral side of parenchymal cells and not on the apical membranes. Abomasal infusion of casein hydrolysate caused a marked change in the profile of arterial blood free amino acids and peptide-bound amino acids smaller than 1500Da. Abundance of aminopeptidase N mRNA and protein increased by 51 and 58%, respectively, in casein hydrolysate-infused goats compared with the control treatment. It was concluded that aminopeptidase N is one candidate actively involved in the mammary gland to support protein synthesis and milk production. In accordance with the nutritional conditions in the current experiment, it is suggested that aminopeptidase N expression is partly controlled by the metabolic requirements of the gland and postabsorptive forms of amino acids in the circulation.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.S0022-0302(05)72882-4</identifier><identifier>PMID: 15905436</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>Savoy, IL: Elsevier Inc</publisher><subject>Amino Acids - blood ; aminopeptidase N ; Animal productions ; Animals ; Biological and medical sciences ; blood composition ; Blotting, Western ; Caseins - administration & dosage ; CD13 Antigens - analysis ; CD13 Antigens - genetics ; CD13 Antigens - physiology ; Diet ; Eating ; Female ; Food industries ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; gene expression regulation ; goats ; Goats - blood ; Goats - metabolism ; Lactation ; mammary gland ; Mammary Glands, Animal - enzymology ; membrane alanyl aminopeptidase ; Milk and cheese industries. Ice creams ; milk synthesis ; peptide ; Peptides - blood ; Protein Biosynthesis ; Terrestrial animal productions ; Vertebrates</subject><ispartof>Journal of dairy science, 2005-06, Vol.88 (6), p.2055-2064</ispartof><rights>2005 American Dairy Science Association</rights><rights>2005 INIST-CNRS</rights><rights>Copyright American Dairy Science Association Jun 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-9a52f7d81c55ff0480ae5b0443a42d8bdd3bd78e8aa3863432741fc17a81c613</citedby><cites>FETCH-LOGICAL-c569t-9a52f7d81c55ff0480ae5b0443a42d8bdd3bd78e8aa3863432741fc17a81c613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3168/jds.S0022-0302(05)72882-4$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16771816$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15905436$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mabjeesh, S. J</creatorcontrib><creatorcontrib>Gal-Garber, O</creatorcontrib><creatorcontrib>Milgram, J</creatorcontrib><creatorcontrib>Feuermann, Y</creatorcontrib><creatorcontrib>Cohen-Zinder, M</creatorcontrib><creatorcontrib>Shamay, A</creatorcontrib><title>Aminopeptidase N Gene Expression and Abundance in Caprine Mammary Gland is Influenced by Circulating Plasma Peptide</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>This study examined the localization and the effect of circulating peptides on the expression of aminopeptidase N (EC 3.4.11.2) in caprine mammary gland. Four lactating goats in mid to late lactation were used in a crossover design and were subjected to 2 dietary treatments. Abomasal infusion of casein hydrolysate was used to increase the concentration of peptide-bound amino acid in the circulation. Samples of mammary gland tissue from each goat were taken by biopsy at the end of each treatment period to measure gene and protein expression of aminopeptidase N in the tissue. There were no measurable effects on feed intake and milk production for any of the treatments. Western blot analysis showed that aminopeptidase N is located on the basolateral side of parenchymal cells and not on the apical membranes. Abomasal infusion of casein hydrolysate caused a marked change in the profile of arterial blood free amino acids and peptide-bound amino acids smaller than 1500Da. Abundance of aminopeptidase N mRNA and protein increased by 51 and 58%, respectively, in casein hydrolysate-infused goats compared with the control treatment. It was concluded that aminopeptidase N is one candidate actively involved in the mammary gland to support protein synthesis and milk production. In accordance with the nutritional conditions in the current experiment, it is suggested that aminopeptidase N expression is partly controlled by the metabolic requirements of the gland and postabsorptive forms of amino acids in the circulation.</description><subject>Amino Acids - blood</subject><subject>aminopeptidase N</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>blood composition</subject><subject>Blotting, Western</subject><subject>Caseins - administration & dosage</subject><subject>CD13 Antigens - analysis</subject><subject>CD13 Antigens - genetics</subject><subject>CD13 Antigens - physiology</subject><subject>Diet</subject><subject>Eating</subject><subject>Female</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>gene expression regulation</subject><subject>goats</subject><subject>Goats - blood</subject><subject>Goats - metabolism</subject><subject>Lactation</subject><subject>mammary gland</subject><subject>Mammary Glands, Animal - enzymology</subject><subject>membrane alanyl aminopeptidase</subject><subject>Milk and cheese industries. Ice creams</subject><subject>milk synthesis</subject><subject>peptide</subject><subject>Peptides - blood</subject><subject>Protein Biosynthesis</subject><subject>Terrestrial animal productions</subject><subject>Vertebrates</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkk1v1DAQhiMEotvCXwCDBIJDij8T-7iKylKpQKWWs-XEztarxEntDbT_nsnuipW4wCVWpGfe8czjLHtD8Dkjhfy0sen8BmNKc8ww_YDFx5JKSXP-JFsQQUXOiJJPs8Uf5CQ7TWkDv4Ri8Tw7IUJhwVmxyNKy92EY3bj11iSHvqGVCw5dPIzRpeSHgEywaFlPwZrQOOQDqswYPTBfTd-b-IhW3Yz4hC5D200OKIvqR1T52Eyd2fqwRtedSb1B17s27kX2rDVdci8P51l2-_nitvqSX31fXVbLq7wRhdrmygjallaSRoi2xVxi40SNOWeGUytra1ltS-mkMUwWjDNactI2pDRQUhB2lr3fx45xuJ9c2urep8Z1cF03TEkXpeSMC_FPkChFlRIYwLd_gZthigFmAEZIKnE5p6k91MQhpehaDeuaF6UJ1rM-Dfr0Tp-e3Wgs9E6f5lD76tBgqntnj5UHXwC8OwAmNaZrI0jx6cgVZUkkKY4j3fn13S8fnYb9dx3Ekrm9lLrQ8BTm277eg60ZtFlHCPtxQzFhGCtVwAeIak84UPXTu6hT43eWIbbZajv4_5jsN343z2I</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Mabjeesh, S. 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J ; Gal-Garber, O ; Milgram, J ; Feuermann, Y ; Cohen-Zinder, M ; Shamay, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-9a52f7d81c55ff0480ae5b0443a42d8bdd3bd78e8aa3863432741fc17a81c613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acids - blood</topic><topic>aminopeptidase N</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>blood composition</topic><topic>Blotting, Western</topic><topic>Caseins - administration & dosage</topic><topic>CD13 Antigens - analysis</topic><topic>CD13 Antigens - genetics</topic><topic>CD13 Antigens - physiology</topic><topic>Diet</topic><topic>Eating</topic><topic>Female</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>gene expression regulation</topic><topic>goats</topic><topic>Goats - blood</topic><topic>Goats - metabolism</topic><topic>Lactation</topic><topic>mammary gland</topic><topic>Mammary Glands, Animal - enzymology</topic><topic>membrane alanyl aminopeptidase</topic><topic>Milk and cheese industries. Ice creams</topic><topic>milk synthesis</topic><topic>peptide</topic><topic>Peptides - blood</topic><topic>Protein Biosynthesis</topic><topic>Terrestrial animal productions</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mabjeesh, S. J</creatorcontrib><creatorcontrib>Gal-Garber, O</creatorcontrib><creatorcontrib>Milgram, J</creatorcontrib><creatorcontrib>Feuermann, Y</creatorcontrib><creatorcontrib>Cohen-Zinder, M</creatorcontrib><creatorcontrib>Shamay, A</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Engineering Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>SIRS Editorial</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mabjeesh, S. J</au><au>Gal-Garber, O</au><au>Milgram, J</au><au>Feuermann, Y</au><au>Cohen-Zinder, M</au><au>Shamay, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aminopeptidase N Gene Expression and Abundance in Caprine Mammary Gland is Influenced by Circulating Plasma Peptide</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>88</volume><issue>6</issue><spage>2055</spage><epage>2064</epage><pages>2055-2064</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><coden>JDSCAE</coden><abstract>This study examined the localization and the effect of circulating peptides on the expression of aminopeptidase N (EC 3.4.11.2) in caprine mammary gland. Four lactating goats in mid to late lactation were used in a crossover design and were subjected to 2 dietary treatments. Abomasal infusion of casein hydrolysate was used to increase the concentration of peptide-bound amino acid in the circulation. Samples of mammary gland tissue from each goat were taken by biopsy at the end of each treatment period to measure gene and protein expression of aminopeptidase N in the tissue. There were no measurable effects on feed intake and milk production for any of the treatments. Western blot analysis showed that aminopeptidase N is located on the basolateral side of parenchymal cells and not on the apical membranes. Abomasal infusion of casein hydrolysate caused a marked change in the profile of arterial blood free amino acids and peptide-bound amino acids smaller than 1500Da. Abundance of aminopeptidase N mRNA and protein increased by 51 and 58%, respectively, in casein hydrolysate-infused goats compared with the control treatment. It was concluded that aminopeptidase N is one candidate actively involved in the mammary gland to support protein synthesis and milk production. In accordance with the nutritional conditions in the current experiment, it is suggested that aminopeptidase N expression is partly controlled by the metabolic requirements of the gland and postabsorptive forms of amino acids in the circulation.</abstract><cop>Savoy, IL</cop><pub>Elsevier Inc</pub><pmid>15905436</pmid><doi>10.3168/jds.S0022-0302(05)72882-4</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of dairy science, 2005-06, Vol.88 (6), p.2055-2064 |
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| subjects | Amino Acids - blood aminopeptidase N Animal productions Animals Biological and medical sciences blood composition Blotting, Western Caseins - administration & dosage CD13 Antigens - analysis CD13 Antigens - genetics CD13 Antigens - physiology Diet Eating Female Food industries Fundamental and applied biological sciences. Psychology Gene Expression gene expression regulation goats Goats - blood Goats - metabolism Lactation mammary gland Mammary Glands, Animal - enzymology membrane alanyl aminopeptidase Milk and cheese industries. Ice creams milk synthesis peptide Peptides - blood Protein Biosynthesis Terrestrial animal productions Vertebrates |
| title | Aminopeptidase N Gene Expression and Abundance in Caprine Mammary Gland is Influenced by Circulating Plasma Peptide |
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