Development and evaluation of a colorimetric membrane-array method for the detection of circulating tumor cells in the peripheral blood of Taiwanese patients with colorectal cancer
Early detection is the hallmark of successful cancer treatment. Evidence is accumulating that primary cancers begin shedding neoplastic cells in the circulation at an early stage. To date, a high-sensitivity and high-throughput method for the detection of circulating tumor cells (CTCs) is deficient....
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| Veröffentlicht in: | International journal of molecular medicine 2006-05, Vol.17 (5), p.737-747 |
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| creator | Wang, Jaw-Yuan Yeh, Ching-Sheng Chen, Yi-Fang Wu, Chan-Hang Hsieh, Jan-Sing Huang, Tsung-Jan Huang, Sung-Yu Lin, Shiu-Ru |
| description | Early detection is the hallmark of successful cancer treatment. Evidence
is accumulating that primary cancers begin shedding neoplastic cells in the circulation
at an early stage. To date, a high-sensitivity and high-throughput method for
the detection of circulating tumor cells (CTCs) is deficient. In this study, we
have developed a high-sensitivity colorimetric membrane-array method to detect
CTCs in the peripheral blood of colorectal cancer (CRC) patients as a potential
diagnostic tool. Previously, we identified a set of 18 oligonucleotide clones,
significantly overexpressed in CRC, which were synthesized and applied to a nylon
membrane. Digoxigenin (DIG)-labeled cDNA were amplified by reverse transcriptase-polymerase
chain reaction (RT-PCR) from the peripheral blood of 88 Taiwanese CRC patients
and 50 healthy subjects, and were then hybridized to the membrane-array. Hybridization
signals were detected by color development. Meanwhile, blood samples were analyzed
by real-time quantitative PCR (Q-PCR). Subsequently, both methods were compared
regarding their correlation, sensitivity and specificity in the detection of CTCs
by statistics. The results of membrane-arrays were demonstrated to be closely
related to that of Q-PCR (P |
| doi_str_mv | 10.3892/ijmm.17.5.737 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67839902</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67839902</sourcerecordid><originalsourceid>FETCH-LOGICAL-c366t-caefa07bded499ef54a31b44e09a0f1597397f650ffa82da93ce7c9e341b66953</originalsourceid><addsrcrecordid>eNpFkc1u3CAURlHVqEnTLrutWHVTeQIGzLCskv5JkbpJpO4QxpcOkW1cwInyXn3A3OlMlBUgzj364CPkA2cbsTXtRbybpg3XG7XRQr8iZ1wb3rRS_n6Ne850I7TqTsnbUu4Ya5U02zfklHfKdK1SZ-TfFdzDmJYJ5krdPFC4d-PqakwzTYE66tOYcpyg5ujpBFOf3QyNy9k94rHu0kBDyrTugA5QwT9P-pj9OqJo_kPrOiHiYRwLjfN_doEclx1kN9J-TCjBkRsXH1Be8BbnMFChD7HuDhHQjKx3s4f8jpwENxZ4f1zPye23rzeXP5rrX99_Xn65brzoutp4B8Ex3Q8wSGMgKOkE76UEZhwLXBktjA6dYiG4bTs4Izxob0BI3nedUeKcfDp4l5z-rlCqnWLZPwNTprXYTm-FMaxFsDmAPqdSMgS74J-5_Gg5s_ua7L4my7VVFmtC_uNRvPYTDC_0sRcEPh-AsmApcUjlhXkulWuFLi21eAIHWqHc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67839902</pqid></control><display><type>article</type><title>Development and evaluation of a colorimetric membrane-array method for the detection of circulating tumor cells in the peripheral blood of Taiwanese patients with colorectal cancer</title><source>Spandidos Publications Journals</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Wang, Jaw-Yuan ; Yeh, Ching-Sheng ; Chen, Yi-Fang ; Wu, Chan-Hang ; Hsieh, Jan-Sing ; Huang, Tsung-Jan ; Huang, Sung-Yu ; Lin, Shiu-Ru</creator><creatorcontrib>Wang, Jaw-Yuan ; Yeh, Ching-Sheng ; Chen, Yi-Fang ; Wu, Chan-Hang ; Hsieh, Jan-Sing ; Huang, Tsung-Jan ; Huang, Sung-Yu ; Lin, Shiu-Ru</creatorcontrib><description>Early detection is the hallmark of successful cancer treatment. Evidence
is accumulating that primary cancers begin shedding neoplastic cells in the circulation
at an early stage. To date, a high-sensitivity and high-throughput method for
the detection of circulating tumor cells (CTCs) is deficient. In this study, we
have developed a high-sensitivity colorimetric membrane-array method to detect
CTCs in the peripheral blood of colorectal cancer (CRC) patients as a potential
diagnostic tool. Previously, we identified a set of 18 oligonucleotide clones,
significantly overexpressed in CRC, which were synthesized and applied to a nylon
membrane. Digoxigenin (DIG)-labeled cDNA were amplified by reverse transcriptase-polymerase
chain reaction (RT-PCR) from the peripheral blood of 88 Taiwanese CRC patients
and 50 healthy subjects, and were then hybridized to the membrane-array. Hybridization
signals were detected by color development. Meanwhile, blood samples were analyzed
by real-time quantitative PCR (Q-PCR). Subsequently, both methods were compared
regarding their correlation, sensitivity and specificity in the detection of CTCs
by statistics. The results of membrane-arrays were demonstrated to be closely
related to that of Q-PCR (P<0.001). The sensitivity and specificity of membrane-arrays
for the detection of CTCs were 94.3% (95% CI, 86.4-102.2%) and 94% (95% CI, 85.9-102.1%),
respectively. Moreover, the accuracy of membrane-arrays is higher than that of
any one gene by Q-PCR. The detection rate of membrane-arrays was significantly
associated with the depth of tumor invasion (P=0.002), the presence of lymph node
metastasis (P=0.016), and TNM stage (P=0.005). The preliminary results indicated
that the accuracy of membrane-arrays was sufficient to distinguish Taiwanese CRC
patients from normal individuals with the advantages of time-saving, cost-effectiveness
and high-throughput. Thus, the constructed colorimetric membrane-array could be
a promising approach for the future detection of CTCs.</description><identifier>ISSN: 1107-3756</identifier><identifier>EISSN: 1791-244X</identifier><identifier>DOI: 10.3892/ijmm.17.5.737</identifier><identifier>PMID: 16596255</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Colorectal Neoplasms - blood ; Colorectal Neoplasms - diagnosis ; Colorectal Neoplasms - genetics ; Digoxigenin - chemistry ; Female ; Gene Expression Profiling - instrumentation ; Gene Expression Profiling - methods ; Gene Expression Regulation, Neoplastic - genetics ; Humans ; Linear Models ; Male ; Middle Aged ; Neoplastic Cells, Circulating - metabolism ; Neoplastic Cells, Circulating - pathology ; Oligonucleotide Array Sequence Analysis - instrumentation ; Oligonucleotide Array Sequence Analysis - methods ; Reproducibility of Results ; Sensitivity and Specificity ; Taiwan</subject><ispartof>International journal of molecular medicine, 2006-05, Vol.17 (5), p.737-747</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-caefa07bded499ef54a31b44e09a0f1597397f650ffa82da93ce7c9e341b66953</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,5556,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16596255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jaw-Yuan</creatorcontrib><creatorcontrib>Yeh, Ching-Sheng</creatorcontrib><creatorcontrib>Chen, Yi-Fang</creatorcontrib><creatorcontrib>Wu, Chan-Hang</creatorcontrib><creatorcontrib>Hsieh, Jan-Sing</creatorcontrib><creatorcontrib>Huang, Tsung-Jan</creatorcontrib><creatorcontrib>Huang, Sung-Yu</creatorcontrib><creatorcontrib>Lin, Shiu-Ru</creatorcontrib><title>Development and evaluation of a colorimetric membrane-array method for the detection of circulating tumor cells in the peripheral blood of Taiwanese patients with colorectal cancer</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Early detection is the hallmark of successful cancer treatment. Evidence
is accumulating that primary cancers begin shedding neoplastic cells in the circulation
at an early stage. To date, a high-sensitivity and high-throughput method for
the detection of circulating tumor cells (CTCs) is deficient. In this study, we
have developed a high-sensitivity colorimetric membrane-array method to detect
CTCs in the peripheral blood of colorectal cancer (CRC) patients as a potential
diagnostic tool. Previously, we identified a set of 18 oligonucleotide clones,
significantly overexpressed in CRC, which were synthesized and applied to a nylon
membrane. Digoxigenin (DIG)-labeled cDNA were amplified by reverse transcriptase-polymerase
chain reaction (RT-PCR) from the peripheral blood of 88 Taiwanese CRC patients
and 50 healthy subjects, and were then hybridized to the membrane-array. Hybridization
signals were detected by color development. Meanwhile, blood samples were analyzed
by real-time quantitative PCR (Q-PCR). Subsequently, both methods were compared
regarding their correlation, sensitivity and specificity in the detection of CTCs
by statistics. The results of membrane-arrays were demonstrated to be closely
related to that of Q-PCR (P<0.001). The sensitivity and specificity of membrane-arrays
for the detection of CTCs were 94.3% (95% CI, 86.4-102.2%) and 94% (95% CI, 85.9-102.1%),
respectively. Moreover, the accuracy of membrane-arrays is higher than that of
any one gene by Q-PCR. The detection rate of membrane-arrays was significantly
associated with the depth of tumor invasion (P=0.002), the presence of lymph node
metastasis (P=0.016), and TNM stage (P=0.005). The preliminary results indicated
that the accuracy of membrane-arrays was sufficient to distinguish Taiwanese CRC
patients from normal individuals with the advantages of time-saving, cost-effectiveness
and high-throughput. Thus, the constructed colorimetric membrane-array could be
a promising approach for the future detection of CTCs.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Colorectal Neoplasms - blood</subject><subject>Colorectal Neoplasms - diagnosis</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Digoxigenin - chemistry</subject><subject>Female</subject><subject>Gene Expression Profiling - instrumentation</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation, Neoplastic - genetics</subject><subject>Humans</subject><subject>Linear Models</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplastic Cells, Circulating - metabolism</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Oligonucleotide Array Sequence Analysis - instrumentation</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Taiwan</subject><issn>1107-3756</issn><issn>1791-244X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1u3CAURlHVqEnTLrutWHVTeQIGzLCskv5JkbpJpO4QxpcOkW1cwInyXn3A3OlMlBUgzj364CPkA2cbsTXtRbybpg3XG7XRQr8iZ1wb3rRS_n6Ne850I7TqTsnbUu4Ya5U02zfklHfKdK1SZ-TfFdzDmJYJ5krdPFC4d-PqakwzTYE66tOYcpyg5ujpBFOf3QyNy9k94rHu0kBDyrTugA5QwT9P-pj9OqJo_kPrOiHiYRwLjfN_doEclx1kN9J-TCjBkRsXH1Be8BbnMFChD7HuDhHQjKx3s4f8jpwENxZ4f1zPye23rzeXP5rrX99_Xn65brzoutp4B8Ex3Q8wSGMgKOkE76UEZhwLXBktjA6dYiG4bTs4Izxob0BI3nedUeKcfDp4l5z-rlCqnWLZPwNTprXYTm-FMaxFsDmAPqdSMgS74J-5_Gg5s_ua7L4my7VVFmtC_uNRvPYTDC_0sRcEPh-AsmApcUjlhXkulWuFLi21eAIHWqHc</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Wang, Jaw-Yuan</creator><creator>Yeh, Ching-Sheng</creator><creator>Chen, Yi-Fang</creator><creator>Wu, Chan-Hang</creator><creator>Hsieh, Jan-Sing</creator><creator>Huang, Tsung-Jan</creator><creator>Huang, Sung-Yu</creator><creator>Lin, Shiu-Ru</creator><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>Development and evaluation of a colorimetric membrane-array method for the detection of circulating tumor cells in the peripheral blood of Taiwanese patients with colorectal cancer</title><author>Wang, Jaw-Yuan ; Yeh, Ching-Sheng ; Chen, Yi-Fang ; Wu, Chan-Hang ; Hsieh, Jan-Sing ; Huang, Tsung-Jan ; Huang, Sung-Yu ; Lin, Shiu-Ru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-caefa07bded499ef54a31b44e09a0f1597397f650ffa82da93ce7c9e341b66953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Colorectal Neoplasms - blood</topic><topic>Colorectal Neoplasms - diagnosis</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Digoxigenin - chemistry</topic><topic>Female</topic><topic>Gene Expression Profiling - instrumentation</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation, Neoplastic - genetics</topic><topic>Humans</topic><topic>Linear Models</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplastic Cells, Circulating - metabolism</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Oligonucleotide Array Sequence Analysis - instrumentation</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Taiwan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jaw-Yuan</creatorcontrib><creatorcontrib>Yeh, Ching-Sheng</creatorcontrib><creatorcontrib>Chen, Yi-Fang</creatorcontrib><creatorcontrib>Wu, Chan-Hang</creatorcontrib><creatorcontrib>Hsieh, Jan-Sing</creatorcontrib><creatorcontrib>Huang, Tsung-Jan</creatorcontrib><creatorcontrib>Huang, Sung-Yu</creatorcontrib><creatorcontrib>Lin, Shiu-Ru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jaw-Yuan</au><au>Yeh, Ching-Sheng</au><au>Chen, Yi-Fang</au><au>Wu, Chan-Hang</au><au>Hsieh, Jan-Sing</au><au>Huang, Tsung-Jan</au><au>Huang, Sung-Yu</au><au>Lin, Shiu-Ru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and evaluation of a colorimetric membrane-array method for the detection of circulating tumor cells in the peripheral blood of Taiwanese patients with colorectal cancer</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>17</volume><issue>5</issue><spage>737</spage><epage>747</epage><pages>737-747</pages><issn>1107-3756</issn><eissn>1791-244X</eissn><abstract>Early detection is the hallmark of successful cancer treatment. Evidence
is accumulating that primary cancers begin shedding neoplastic cells in the circulation
at an early stage. To date, a high-sensitivity and high-throughput method for
the detection of circulating tumor cells (CTCs) is deficient. In this study, we
have developed a high-sensitivity colorimetric membrane-array method to detect
CTCs in the peripheral blood of colorectal cancer (CRC) patients as a potential
diagnostic tool. Previously, we identified a set of 18 oligonucleotide clones,
significantly overexpressed in CRC, which were synthesized and applied to a nylon
membrane. Digoxigenin (DIG)-labeled cDNA were amplified by reverse transcriptase-polymerase
chain reaction (RT-PCR) from the peripheral blood of 88 Taiwanese CRC patients
and 50 healthy subjects, and were then hybridized to the membrane-array. Hybridization
signals were detected by color development. Meanwhile, blood samples were analyzed
by real-time quantitative PCR (Q-PCR). Subsequently, both methods were compared
regarding their correlation, sensitivity and specificity in the detection of CTCs
by statistics. The results of membrane-arrays were demonstrated to be closely
related to that of Q-PCR (P<0.001). The sensitivity and specificity of membrane-arrays
for the detection of CTCs were 94.3% (95% CI, 86.4-102.2%) and 94% (95% CI, 85.9-102.1%),
respectively. Moreover, the accuracy of membrane-arrays is higher than that of
any one gene by Q-PCR. The detection rate of membrane-arrays was significantly
associated with the depth of tumor invasion (P=0.002), the presence of lymph node
metastasis (P=0.016), and TNM stage (P=0.005). The preliminary results indicated
that the accuracy of membrane-arrays was sufficient to distinguish Taiwanese CRC
patients from normal individuals with the advantages of time-saving, cost-effectiveness
and high-throughput. Thus, the constructed colorimetric membrane-array could be
a promising approach for the future detection of CTCs.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>16596255</pmid><doi>10.3892/ijmm.17.5.737</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1107-3756 |
| ispartof | International journal of molecular medicine, 2006-05, Vol.17 (5), p.737-747 |
| issn | 1107-3756 1791-244X |
| language | eng |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adult Aged Aged, 80 and over Colorectal Neoplasms - blood Colorectal Neoplasms - diagnosis Colorectal Neoplasms - genetics Digoxigenin - chemistry Female Gene Expression Profiling - instrumentation Gene Expression Profiling - methods Gene Expression Regulation, Neoplastic - genetics Humans Linear Models Male Middle Aged Neoplastic Cells, Circulating - metabolism Neoplastic Cells, Circulating - pathology Oligonucleotide Array Sequence Analysis - instrumentation Oligonucleotide Array Sequence Analysis - methods Reproducibility of Results Sensitivity and Specificity Taiwan |
| title | Development and evaluation of a colorimetric membrane-array method for the detection of circulating tumor cells in the peripheral blood of Taiwanese patients with colorectal cancer |
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