Cloning and characterization of a carboxylesterase from Bacillus coagulans 81-11

A genomic library of Bacillus coagulans strain 81-11 was screened in Escherichia coli JM83 for lipolytic activity by using tributyrin agar plates. A 2.4 kb DNA fragment was subcloned from a lipolytic-positive clone and completely sequenced. Nucleotide sequence analysis predicted a 723 bp open readin...

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Veröffentlicht in:	Current microbiology 2005-04, Vol.50 (4), p.196-201
Hauptverfasser:	Mnisi, Stephens M, Louw, Maureen E, Theron, Jacques
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Bacillus - enzymology Bacillus - genetics Bacillus coagulans Bacteria Bacteriology Carboxylesterase - chemistry Carboxylesterase - genetics Carboxylesterase - metabolism Cloning Cloning, Molecular DNA, Bacterial - analysis DNA, Bacterial - genetics E coli Enzyme Stability Enzymes Escherichia coli Esters Genome, Bacterial Geobacillus Microbiology Molecular Sequence Data
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